BBa_K1020012
type: ComponentDefinition
persistent identity: BBa K1020012
display id: BBa_K1020012
version: 1
was generated by: iGEM to SBOL conversion
description: Alkane Selector for intracellular alkane production
created: 2013-09-09T11:00:00Z
modified: 2015-05-08T01:08:43Z
owned by: james
owned by: myers
discontinued: false
dominant: false
experience: experience/Works
group u list: _1327_
m user id: 0
owner id: 16881
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
star: false
creator: Yue Liu
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_K1020003
component: BBa_K1020010
sequence annotation: BBa_K1020010
sequence annotation: BBa_K1020003
sequence: https://synbiohub.org/public/igem/BBa_K1020012_sequence/1

BBa_K1020003
type: Component
persistent identity: component2373802
display id: component2373802
version: 1
top level: BBa_K1020012
definition: BBa_K1020003
access: public

BBa_K1020010
type: Component
persistent identity: component2373789
display id: component2373789
version: 1
top level: BBa_K1020012
definition: PalkM-tetA
access: public

BBa_K1020010
type: SequenceAnnotation
persistent identity: annotation2373789
display id: annotation2373789
version: 1
top level: BBa_K1020012
location: https://synbiohub.org/public/igem/BBa_K1020012/annotation2373789/range2373789/1
component: BBa_K1020010

https://synbiohub.org/public/igem/BBa_K1020012/annotation2373789/range2373789/1
type: Range
persistent identity: range2373789
display id: range2373789
version: 1
top level: BBa_K1020012
direction: direction/reverse
start: 1
end: 1529
orientation: inline

BBa_K1020003
type: SequenceAnnotation
persistent identity: annotation2373802
display id: annotation2373802
version: 1
top level: BBa_K1020012
location: https://synbiohub.org/public/igem/BBa_K1020012/annotation2373802/range2373802/1
component: BBa_K1020003

https://synbiohub.org/public/igem/BBa_K1020012/annotation2373802/range2373802/1
type: Range
persistent identity: range2373802
display id: range2373802
version: 1
top level: BBa_K1020012
direction: direction/reverse
start: 1538
end: 2662
orientation: inline

BBa_K1020003
type: ComponentDefinition
persistent identity: BBa K1020003
display id: BBa_K1020003
version: 1
was generated by: iGEM to SBOL conversion
description: AlkR -PSB1C3-Low constitutive
created: 2013-08-17T11:00:00Z
modified: 2015-05-08T01:08:43Z
mutable provenance: Acinetobacter baylyi ADP1
owned by: james
owned by: myers
mutable description: AlkR is a transcriptional regulator belonging to AraC/Xyls family, which could detect a broad range of alkanes and alkenes with carbon chain length from C7 to C36. It is said that AlkR is the only bioreporter that is able to detect alkane with carbon chain length greater than C18. The mechanism is that alkanes are presumably recognized by AlkR regulatory protein and their interaction triggered a conformation change of AlkR dimers which isomerizes the promoter-RNAP complex and lead to the activation of alkM gene. AlkR regulatory protein includes C-terminal DNA-binding domain(CTD) for promoter binding and N-terminal domain(NTD) for inducer recognition. Here we use a constitutive promoter for AlkR gene and in case of interference, we choose a long and strong terminator.
discontinued: false
dominant: false
experience: experience/Works
group u list: _1327_
m user id: 0
owner id: 16881
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: We use a constitutive promoter for AlkR gene and we choose a long and strong terminator to prevent interference.
star: false
creator: Yue Liu
type: DnaRegion
role: SO:0000804
role: partType/Reporter
component: BBa_B0015
component: BBa_K1020002
component: BBa_B0034
component: BBa_J23103
sequence annotation: BBa_J23103
sequence annotation: BBa_B0034
sequence annotation: BBa_K1020002
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_K1020003_sequence/1

BBa_B0015
type: Component
persistent identity: component2333548
display id: component2333548
version: 1
top level: BBa_K1020003
definition: BBa_B0015
access: public

BBa_K1020002
type: Component
persistent identity: component2333541
display id: component2333541
version: 1
top level: BBa_K1020003
definition: BBa_K1020002
access: public

BBa_B0034
type: Component
persistent identity: component2333539
display id: component2333539
version: 1
top level: BBa_K1020003
definition: BBa_B0034
access: public

BBa_J23103
type: Component
persistent identity: component2333537
display id: component2333537
version: 1
top level: BBa_K1020003
definition: BBa_J23103
access: public

BBa_J23103
type: SequenceAnnotation
persistent identity: annotation2333537
display id: annotation2333537
version: 1
top level: BBa_K1020003
location: https://synbiohub.org/public/igem/BBa_K1020003/annotation2333537/range2333537/1
component: BBa_J23103

https://synbiohub.org/public/igem/BBa_K1020003/annotation2333537/range2333537/1
type: Range
persistent identity: range2333537
display id: range2333537
version: 1
top level: BBa_K1020003
direction: direction/reverse
start: 1
end: 35
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2333539
display id: annotation2333539
version: 1
top level: BBa_K1020003
location: https://synbiohub.org/public/igem/BBa_K1020003/annotation2333539/range2333539/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1020003/annotation2333539/range2333539/1
type: Range
persistent identity: range2333539
display id: range2333539
version: 1
top level: BBa_K1020003
direction: direction/reverse
start: 44
end: 55
orientation: inline

BBa_K1020002
type: SequenceAnnotation
persistent identity: annotation2333541
display id: annotation2333541
version: 1
top level: BBa_K1020003
location: https://synbiohub.org/public/igem/BBa_K1020003/annotation2333541/range2333541/1
component: BBa_K1020002

https://synbiohub.org/public/igem/BBa_K1020003/annotation2333541/range2333541/1
type: Range
persistent identity: range2333541
display id: range2333541
version: 1
top level: BBa_K1020003
direction: direction/reverse
start: 62
end: 988
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2333548
display id: annotation2333548
version: 1
top level: BBa_K1020003
location: https://synbiohub.org/public/igem/BBa_K1020003/annotation2333548/range2333548/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K1020003/annotation2333548/range2333548/1
type: Range
persistent identity: range2333548
display id: range2333548
version: 1
top level: BBa_K1020003
direction: direction/reverse
start: 997
end: 1125
orientation: inline

PalkM-tetA
type: ComponentDefinition
persistent identity: BBa K1020010
display id: BBa_K1020010
version: 1
was generated by: iGEM to SBOL conversion
description: TetA gene under the control of PalkM
created: 2013-09-09T11:00:00Z
modified: 2015-05-08T01:08:43Z
owned by: james
owned by: myers
mutable description: The parts including two core enzymes to produce alkane.
discontinued: false
dominant: false
experience: experience/Works
group u list: _1327_
m user id: 0
owner id: 16881
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
star: false
creator: Yue Liu
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: PalkM promoter
sequence annotation: tetA
sequence annotation: terminator
sequence: https://synbiohub.org/public/igem/BBa_K1020010_sequence/1

PalkM promoter
type: SequenceAnnotation
persistent identity: annotation2353542
display id: annotation2353542
version: 1
top level: PalkM-tetA
location: https://synbiohub.org/public/igem/BBa_K1020010/annotation2353542/range2353542/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_K1020010/annotation2353542/range2353542/1
type: Range
persistent identity: range2353542
display id: range2353542
version: 1
top level: PalkM-tetA
direction: direction/forward
start: 1
end: 178
orientation: inline

tetA
type: SequenceAnnotation
persistent identity: annotation2353543
display id: annotation2353543
version: 1
top level: PalkM-tetA
location: https://synbiohub.org/public/igem/BBa_K1020010/annotation2353543/range2353543/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_K1020010/annotation2353543/range2353543/1
type: Range
persistent identity: range2353543
display id: range2353543
version: 1
top level: PalkM-tetA
direction: direction/forward
start: 203
end: 1394
orientation: inline

terminator
type: SequenceAnnotation
persistent identity: annotation2353556
display id: annotation2353556
version: 1
top level: PalkM-tetA
location: https://synbiohub.org/public/igem/BBa_K1020010/annotation2353556/range2353556/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_K1020010/annotation2353556/range2353556/1
type: Range
persistent identity: range2353556
display id: range2353556
version: 1
top level: PalkM-tetA
direction: direction/forward
start: 1400
end: 1529
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

BBa_B0015
type: ComponentDefinition
persistent identity: BBa B0015
display id: BBa_B0015
version: 1
was generated by: iGEM to SBOL conversion
description: double terminator (B0010-B0012)
created: 2003-07-16T11:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Double terminator consisting of BBa_B0010 and BBa_B0012
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Reshma Shetty
type: DnaRegion
role: partType/Terminator
role: SO:0000141
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_B0015_sequence/1

BBa_B0012
type: Component
persistent identity: component1916612
display id: component1916612
version: 1
top level: BBa_B0015
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1916610
display id: component1916610
version: 1
top level: BBa_B0015
definition: BBa_B0010
access: public

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1916610
display id: annotation1916610
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
type: Range
persistent identity: range1916610
display id: range1916610
version: 1
top level: BBa_B0015
direction: direction/forward
start: 1
end: 80
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1916612
display id: annotation1916612
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
type: Range
persistent identity: range1916612
display id: range1916612
version: 1
top level: BBa_B0015
direction: direction/forward
start: 89
end: 129
orientation: inline

BBa_K1020002
type: ComponentDefinition
persistent identity: BBa K1020002
display id: BBa_K1020002
version: 1
was generated by: iGEM to SBOL conversion
description: alkane regulatory protein ALKR
created: 2013-08-17T11:00:00Z
modified: 2015-05-08T01:08:43Z
mutable provenance: Acinetobacter baylyi ADP1
owned by: james
owned by: myers
mutable description: AlkR is a transcriptional regulator belonging to AraC/Xyls family, which could detect a broad range of alkanes and alkenes with carbon chain length from C7 to C36. It is said that AlkR is the only bioreporter that is able to detect alkane with carbon chain length greater than C18.
discontinued: false
dominant: false
experience: experience/Works
group u list: _1327_
m user id: 0
owner id: 16881
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
star: false
creator: Yue Liu
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: AlkR
sequence: https://synbiohub.org/public/igem/BBa_K1020002_sequence/1

AlkR
type: SequenceAnnotation
persistent identity: annotation2331899
display id: annotation2331899
version: 1
top level: BBa_K1020002
location: https://synbiohub.org/public/igem/BBa_K1020002/annotation2331899/range2331899/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_K1020002/annotation2331899/range2331899/1
type: Range
persistent identity: range2331899
display id: range2331899
version: 1
top level: BBa_K1020002
direction: direction/forward
start: 1
end: 927
orientation: inline

BBa_J23103
type: ComponentDefinition
persistent identity: BBa J23103
display id: BBa_J23103
version: 1
was generated by: iGEM to SBOL conversion
description: constitutive promoter family member
created: 2006-08-03T11:00:00Z
modified: 2015-08-31T04:08:40Z
mutable provenance: isolated from library of promoters
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: replace later
discontinued: false
dominant: true
experience: experience/Works
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: true
mutable notes: N/A
star: true
creator: John Anderson
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J23103_sequence/1

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: SO:0000553
role: feature/polya

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

https://synbiohub.org/public/igem/BBa_K1020012_sequence/1
type: Sequence
persistent identity: BBa K1020012 sequence
display id: BBa_K1020012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: agcgcgttaaaaattcttatttgagcaagagtatagaatgaggttgtagcttatacatgtgattttaggacattgacatggttgaaaatgccaaagactttgacataggttttttggacgataacgactgtttttaatatataagatggatgattatgagcagtatgaatagaggataactaggaaagaggagaaatactagatgaaatctaacaatgcgctcatcgtcatcctcggcaccgtcaccctggatgctgtaggcataggcttggttatgccggtactgccgggcctcttgcgggatatcgtccattccgacagcatcgccagtcactatggcgtgctgctagcgctatatgcgttgatgcaatttctatgcgcacccgttctcggagcactgtccgaccgctttggccgccgcccagtcctgctcgcttcgctacttggagccactatcgactacgcgatcatggcgaccacacccgtcctgtggatcctctacgccggacgcatcgtggccggcatcaccggcgccacaggtgcggttgctggcgcctatatcgccgacatcaccgatggggaagatcgggctcgccacttcgggctcatgagcgcttgtttcggcgtgggtatggtggcaggccccgtggccgggggactgttgggcgccatctccttgcatgcaccattccttgcggcggcggtgctcaacggcctcaacctactactgggctgcttcctaatgcaggagtcgcataagggagagcgtcgaccgatgcccttgagagccttcaacccagtcagctccttccggtgggcgcggggcatgactatcgtcgccgcacttatgactgtcttctttatcatgcaactcgtaggacaggtgccggcagcgctctgggtcattttcggcgaggaccgctttcgctggagcgcgacgatgatcggcctgtcgcttgcggtattcggaatcttgcacgccctcgctcaagccttcgtcactggtcccgccaccaaacgtttcggcgagaagcaggccattatcgccggcatggcggccgacgcgctgggctacgtcttgctggcgttcgcgacgcgaggctggatggccttccccattatgattcttctcgcttccggcggcatcgggatgcccgcgttgcaggccatgctgtccaggcaggtagatgacgaccatcagggacagcttcaaggatcgctcgcggctcttaccagcctaacttcgatcactggaccgctgatcgtcacggcgatttatgccgcctcggcgagcacatggaacgggttggcatggattgtaggcgccgccctataccttgtctgcctccccgcgttgcgtcgcggtgcatggagccgggccacctcgacctaaactagaccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatattactagagctgatagctagctcagtcctagggattatgctagctactagagaaagaggagaaatactagatggatgcacttagtaaaatttttgatgatattcatttaaataaatctgaatatctctatgtaaaaactcaaggagaatgggcatttcatatgcttgcccaagatgctttgattgctcacattattttaatgggttctgctcatttcacattacaggatggtacgacacaaaccgcttattcgggagatattgtgcttattccttctgggcaagctcattttgtctcaaatgatgctcagaacaaactaattgattgttccaacattcagtctttttttgatggacaccgcaatgatgccattgagttaggcacaacatcttcggatcatggtttaatttttactgtgcggagccatatcgatatgcatatgggtagccctttactcaatgctttaccatca…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1020003_sequence/1
type: Sequence
persistent identity: BBa K1020003 sequence
display id: BBa_K1020003_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ctgatagctagctcagtcctagggattatgctagctactagagaaagaggagaaatactagatggatgcacttagtaaaatttttgatgatattcatttaaataaatctgaatatctctatgtaaaaactcaaggagaatgggcatttcatatgcttgcccaagatgctttgattgctcacattattttaatgggttctgctcatttcacattacaggatggtacgacacaaaccgcttattcgggagatattgtgcttattccttctgggcaagctcattttgtctcaaatgatgctcagaacaaactaattgattgttccaacattcagtctttttttgatggacaccgcaatgatgccattgagttaggcacaacatcttcggatcatggtttaatttttactgtgcggagccatatcgatatgcatatgggtagccctttactcaatgctttaccatcactgatacatattcatcacgcaatgagctcaactggcccagagtggttacgcgtcggtttatattttgtggcacttgaaacccagcgtattcaaccaggacgagataaaatatttgaccacttgatgagcatcctatttatagaatgtgtccgcgatcatattgcccagcttgatgaccccaaaagctggttaactgcactcatgcatcccgaattatctaatgcgctcgcagctattcatgcttaccccgaaaaaccttggactgtagagagtttggcagatcaatgttgcatgtcacggtctaaatttgccacactttttcaaagtattgttcatgagacccctctcgcttatcttcaacaacatcgccttcgtctggccgtacagttattaaaaaccagtcagttaaatattcagcaaattgccaataaagtcggatactcctcagaaacagcttttagtcaggcatttaaacgtcaatttgaacaatctcctaaacactatcgccagcaatcatgataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1020010_sequence/1
type: Sequence
persistent identity: BBa K1020010 sequence
display id: BBa_K1020010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: agcgcgttaaaaattcttatttgagcaagagtatagaatgaggttgtagcttatacatgtgattttaggacattgacatggttgaaaatgccaaagactttgacataggttttttggacgataacgactgtttttaatatataagatggatgattatgagcagtatgaatagaggataactaggaaagaggagaaatactagatgaaatctaacaatgcgctcatcgtcatcctcggcaccgtcaccctggatgctgtaggcataggcttggttatgccggtactgccgggcctcttgcgggatatcgtccattccgacagcatcgccagtcactatggcgtgctgctagcgctatatgcgttgatgcaatttctatgcgcacccgttctcggagcactgtccgaccgctttggccgccgcccagtcctgctcgcttcgctacttggagccactatcgactacgcgatcatggcgaccacacccgtcctgtggatcctctacgccggacgcatcgtggccggcatcaccggcgccacaggtgcggttgctggcgcctatatcgccgacatcaccgatggggaagatcgggctcgccacttcgggctcatgagcgcttgtttcggcgtgggtatggtggcaggccccgtggccgggggactgttgggcgccatctccttgcatgcaccattccttgcggcggcggtgctcaacggcctcaacctactactgggctgcttcctaatgcaggagtcgcataagggagagcgtcgaccgatgcccttgagagccttcaacccagtcagctccttccggtgggcgcggggcatgactatcgtcgccgcacttatgactgtcttctttatcatgcaactcgtaggacaggtgccggcagcgctctgggtcattttcggcgaggaccgctttcgctggagcgcgacgatgatcggcctgtcgcttgcggtattcggaatcttgcacgccctcgctcaagccttcgtcactggtcccgccaccaaacgtttcggcgagaagcaggccattatcgccggcatggcggccgacgcgctgggctacgtcttgctggcgttcgcgacgcgaggctggatggccttccccattatgattcttctcgcttccggcggcatcgggatgcccgcgttgcaggccatgctgtccaggcaggtagatgacgaccatcagggacagcttcaaggatcgctcgcggctcttaccagcctaacttcgatcactggaccgctgatcgtcacggcgatttatgccgcctcggcgagcacatggaacgggttggcatggattgtaggcgccgccctataccttgtctgcctccccgcgttgcgtcgcggtgcatggagccgggccacctcgacctaaactagaccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatat
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0015_sequence/1
type: Sequence
persistent identity: BBa B0015 sequence
display id: BBa_B0015_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1020002_sequence/1
type: Sequence
persistent identity: BBa K1020002 sequence
display id: BBa_K1020002_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggatgcacttagtaaaatttttgatgatattcatttaaataaatctgaatatctctatgtaaaaactcaaggagaatgggcatttcatatgcttgcccaagatgctttgattgctcacattattttaatgggttctgctcatttcacattacaggatggtacgacacaaaccgcttattcgggagatattgtgcttattccttctgggcaagctcattttgtctcaaatgatgctcagaacaaactaattgattgttccaacattcagtctttttttgatggacaccgcaatgatgccattgagttaggcacaacatcttcggatcatggtttaatttttactgtgcggagccatatcgatatgcatatgggtagccctttactcaatgctttaccatcactgatacatattcatcacgcaatgagctcaactggcccagagtggttacgcgtcggtttatattttgtggcacttgaaacccagcgtattcaaccaggacgagataaaatatttgaccacttgatgagcatcctatttatagaatgtgtccgcgatcatattgcccagcttgatgaccccaaaagctggttaactgcactcatgcatcccgaattatctaatgcgctcgcagctattcatgcttaccccgaaaaaccttggactgtagagagtttggcagatcaatgttgcatgtcacggtctaaatttgccacactttttcaaagtattgttcatgagacccctctcgcttatcttcaacaacatcgccttcgtctggccgtacagttattaaaaaccagtcagttaaatattcagcaaattgccaataaagtcggatactcctcagaaacagcttttagtcaggcatttaaacgtcaatttgaacaatctcctaaacactatcgccagcaatcatgataataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J23103_sequence/1
type: Sequence
persistent identity: BBa J23103 sequence
display id: BBa_J23103_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ctgatagctagctcagtcctagggattatgctagc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: Chris J. Myers
creator: James Alastair McLaughlin
ended at time: 2017-03-06T15:00:00.000Z