BBa_K102031
1
BBa_K102031
TA1 gate in output test harness
2008-10-27T12:00:00Z
2015-05-08T01:08:43Z
Synthetic
The output test harness provides a means to visibly detect when a gate is activated. It consists of a Lac promoter (which is induced by IPTG throughout the test to act as a constitutive promoter) followed by the gate then an RBS and LacZα. In order to test activity the activation of the gate by its correct input is compared to that from a ???null??? input using a standard LacZ assay.
false
false
_181_
0
2647
9
It's complicated
false
See Team wiki (2008 Alberta_NINT) Project page for description of algorithm
false
Wayne Materi
component1991948
1
BBa_B0034
component1991946
1
BBa_K102901
component1991952
1
BBa_I732006
component1991938
1
BBa_R0010
annotation1991952
1
BBa_I732006
range1991952
1
300
533
annotation1991948
1
BBa_B0034
range1991948
1
282
293
annotation1991938
1
BBa_R0010
range1991938
1
1
200
annotation1991946
1
BBa_K102901
range1991946
1
209
273
BBa_K102901
1
BBa_K102901
TA1 (THRU gate)
2008-10-23T11:00:00Z
2015-05-08T01:08:46Z
(Larson et al, 2008)
TA1 is based on the canonical his terminator (Larson et al, 2008) with all U's at the 3' tail.
false
false
_181_
0
2647
9
Not in stock
true
Gate is flanked by BamHI and NcoI to facilitate simple replacement in output test harness. Because gates are fairly long duplex DNA strands, a single strand can be ordered and the dsDNA created by PCR using Term+ (5'cgatcagaattcgcggcc) and Term- (5'GGACATCTGCAGCGGCCG) primers.
false
Wayne Materi
annotation1986806
1
his term
range1986806
1
19
58
BBa_I732006
1
lacZ-alpha
lacZ alpha fragment
2007-07-06T11:00:00Z
2015-08-31T04:07:56Z
PCR amplified from BBa_J33202 without the RBS.
Released HQ 2013
In strains with lacZ-omega (lacZ N-terminal deletion mutant) like DH5alpha, DH10B and Top10, lacZ-alpha restores the beta-galactosidase activity.
false
true
_156_
0
1557
9
In stock
false
None
true
Zhan Jian
annotation1936906
1
STOP
range1936906
1
229
234
annotation1936905
1
START
range1936905
1
1
3
annotation1936904
1
lacZ
range1936904
1
1
234
BBa_R0010
1
LacI
promoter (lacI regulated)
2003-01-31T12:00:00Z
2015-05-08T01:14:14Z
The Plac insert was PCR'd from the MG1655 strain of E.coli K12.
Released HQ 2013
Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) <p> The pLac regulatory region is a 243 base-pair sequence with standard BioBrick prefix and suffix sections on its ends. It contains two protein binding sites: CAP, which is generally present in E.coli and is assocciated with cell health and availability of glucose., and LacI, the Lac inhibitor <bb_part>BBa_C0010</bb_part> which binds in an dimerized cooperative manner to inhibit the transcription of the protein that follows. In the presence of lactose or IPTG, an analog of lactose, LacI is unable to correctly bind and inhibit transcription. This allows <bb_part>BBa_R0010</bb_part> to be used as a inverter or as a detector of lactose or IPTG.
false
true
_1_
0
24
7
In stock
false
<P> <P><P> LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and this regulator. This part is incompatible with environments containing lactose or lactose analogs.
true
annotation1961223
1
CAP binding site
range1961223
1
89
126
annotation1961225
1
-10
range1961225
1
161
166
annotation1961224
1
-35
range1961224
1
137
142
annotation1961222
1
BBa_R0010
range1961222
1
1
200
annotation1961221
1
end of LacI coding region (inactive)
range1961221
1
1
88
annotation1961227
1
start
range1961227
1
173
173
annotation1961226
1
LacI binding site
range1961226
1
166
200
BBa_B0034
1
BBa_B0034
RBS (Elowitz 1999) -- defines RBS efficiency
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
RBS based on Elowitz repressilator.
false
true
_1_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.
Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation23325
1
conserved
range23325
1
5
8
BBa_K102031_sequence
1
caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaatagatcattgggatccgaagcccccggaagatgcatcttccgggggctttttttttccatggttactagagaaagaggagaaatactagatgaccatgattacggattcactggccgtcgttttacaacgtcgtgactgggaaaaccctggcgttacccaacttaatcgccttgcagcacatccccctttcgccagctggcgtaatagcgaagaggcccgcaccgatcgcccttcccaacagttgcgcagcctgaatggcgaatggcgctttgcctggtttccggcaccagaagcggtgccggaaagctggctggagtaataa
BBa_R0010_sequence
1
caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacaca
BBa_B0034_sequence
1
aaagaggagaaa
BBa_K102901_sequence
1
aatagatcattgggatccgaagcccccggaagatgcatcttccgggggctttttttttccatggt
BBa_I732006_sequence
1
atgaccatgattacggattcactggccgtcgttttacaacgtcgtgactgggaaaaccctggcgttacccaacttaatcgccttgcagcacatccccctttcgccagctggcgtaatagcgaagaggcccgcaccgatcgcccttcccaacagttgcgcagcctgaatggcgaatggcgctttgcctggtttccggcaccagaagcggtgccggaaagctggctggagtaataa
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z