BBa_K1021001 1 BBa_K1021001 nptII (neomycin phosphotransferase) 2013-08-29T11:00:00Z 2015-05-08T01:08:43Z pNG, acquired from the lab of B. G. Turgeon and described in "ChLae1 and ChVel1 Regulate T-toxin Production, Virulence, Oxidative Stress Response, and Development of the Maize Pathogen Cochliobolus heterostrophus" (Wu et al. 2012). The nptII gene encodes neomycin phosphotransferase, which confers resistance to geneticin, kanamycin, and related antibiotics. false false _1328_ 0 13516 9 In stock true An illegal PstI site was removed through site-directed mutagenesis. false Swati Sureka annotation2333716 1 nptII range2333716 1 1 795 BBa_K1021008 1 BBa_K1021008 TtrpC 2013-09-12T11:00:00Z 2015-05-08T01:08:43Z Fungal transformation vector pUCATPH (http://www.snapgene.com/resources/plasmid_files/yeast_plasmids/pUCATPH/), described in http://www.pnas.org/content/91/26/12649.full.pdf. This is a fungal terminator from Aspergillus nidulans' tryptophan biosynthesis pathway. false false _1328_ 0 13516 9 In stock false The terminator is best used in conjunction with PtrpC (BBa_K1021007). false Swati Sureka annotation2340038 1 TtrpC range2340038 1 1 568 BBa_K1021007 1 BBa_K1021007 PtrpC 2013-09-12T11:00:00Z 2015-05-08T01:08:43Z Fungal transformation vector pUCATPH (http://www.snapgene.com/resources/plasmid_files/yeast_plasmids/pUCATPH/), described in http://www.pnas.org/content/91/26/12649.full.pdf. This is a strong constitutive promoter from Aspergillus niger's tryptophan biosynthesis pathways. false false _1328_ 0 13516 9 In stock true There was an internal SpeI site near the 3'end that was used in the suffix. false Swati Sureka annotation2340027 1 PtrpC range2340027 1 5 365 BBa_K1021015 1 BBa_K1021015 PtrpC+nptII 2013-09-12T11:00:00Z 2015-05-08T01:08:44Z These originate from the pUCATPH and pNG transformation vectors. The geneticin (G418) resistance gene sits downstream of the constitutive trpC promoter from Aspergillus nidulans. This part can be used as a selectable marker for fungal transformation. false false _1328_ 0 13516 9 It's complicated false Fungi do not use RBS; therefore, this is a complete translational unit. false Swati Sureka component2340341 1 BBa_K1021001 component2340339 1 BBa_K1021007 annotation2340341 1 BBa_K1021001 range2340341 1 376 1176 annotation2340339 1 BBa_K1021007 range2340339 1 1 369 BBa_K1021029 1 BBa_K1021029 PtrpC+nptII+TtrpC 2013-09-12T11:00:00Z 2015-05-08T01:08:44Z PtrpC and TtrpC, both from A. nidulans, came from the pUCATPH transformation vector. The nptII gene from E. coli K12 was taken from the pNG transformation vector. This is the complete generator for constitutive expression of the neomycin phosphotransferase gene in fungi. false false _1328_ 0 13516 9 In stock false The terminator is not strictly necessary in fungi, but was added to improve BBa_K1021015. false Swati Sureka component2340540 1 BBa_K1021008 component2340538 1 BBa_K1021015 annotation2340540 1 BBa_K1021008 range2340540 1 1185 1752 annotation2340538 1 BBa_K1021015 range2340538 1 1 1176 BBa_K1021029_sequence 1 tcgacagaagatgatattgaaggagcactttttgggcttggctggagctagtggaggtcaacaatgaatgcctattttggtttagtcgtccaggcggtgagcacaaaatttgtgtcgtttgacaagatggttcatttaggcaactggtcagatcagccccacttgtagcagtagcggcggcgctcgaagtgtgactcttattagcagacaggaacgaggacattattatcatctgctgcttggtgcacgataacttggtgcgtttgtcaagcaaggtaagtgaacgacccggtcataccttcttaagttcgcccttcctccctttatttcagattcaatctgacttacctattctacccaagcatcgattactagatgattgaacaagatggattgcacgcaggttctccggccgcttgggtggagaggctattcggctatgactgggcacaacagacaatcggctgctctgatgccgccgtgttccggctgtcagcgcaggggcgcccggttctttttgtcaagaccgacctgtccggtgccctgaatgaactccaggacgaggcagcgcggctatcgtggctggccacgacgggcgttccttgcgcagctgtgctcgacgttgtcactgaagcgggaagggactggctgctattgggcgaagtgccggggcaggatctcctgtcatctcaccttgctcctgccgagaaagtatccatcatggctgatgcaatgcggcggctgcatacgcttgatccggctacctgcccattcgaccaccaagcgaaacatcgcatcgagcgagcacgtactcggatggaagccggtcttgtcgatcaggatgatctggacgaagagcatcaggggctcgcgccagccgaactgttcgccaggctcaaggcgcgcatgcccgacggcgaggatctcgtcgtgacccatggcgatgcctgcttgccgaatatcatggtggaaaatggccgcttttctggattcatcgactgtggccggctgggtgtggcggaccgctatcaggacatagcgttggctacccgtgatattgctgaagagcttggcggcgaatgggctgaccgcttcctcgtgctttacggtatcgccgctcccgattcgcagcgcatcgccttctatcgccttcttgacgagttcttctgaggatcctactagagtgatttaatagctccatgtcaacaagaataaaacgcgtttcgggtttacctcttccagatacagctcatctgcaatgcattaatgcattggacctcgcaaccctagtacgcccttcaggctccggcgaagcagaagaatagcttagcagagtctattttcattttcgggagacgagatcaagcagatcaacggtcgtcaagagacctacgagactgaggaatccgctcttggctccacgcgactatatatttgtctctaattgtactttgacatgctcctcttctttactctgatagcttgactatgaaaattccgtcaccagcccctgggttcgcaaagataattgcactgtttcttccttgaactctcaagcctacaggacacacattcatcgtaggtataaacctcgaaaatcattcctactaagatgggtatacaatagtaaccatggttgcctagtgaatgctccgtaacacccaatacgccggccgaaacttttttacaactctcctatgagtcgtttacccagaatgcacaggtacacttgtttagaggtaatccttcttt BBa_K1021008_sequence 1 tgatttaatagctccatgtcaacaagaataaaacgcgtttcgggtttacctcttccagatacagctcatctgcaatgcattaatgcattggacctcgcaaccctagtacgcccttcaggctccggcgaagcagaagaatagcttagcagagtctattttcattttcgggagacgagatcaagcagatcaacggtcgtcaagagacctacgagactgaggaatccgctcttggctccacgcgactatatatttgtctctaattgtactttgacatgctcctcttctttactctgatagcttgactatgaaaattccgtcaccagcccctgggttcgcaaagataattgcactgtttcttccttgaactctcaagcctacaggacacacattcatcgtaggtataaacctcgaaaatcattcctactaagatgggtatacaatagtaaccatggttgcctagtgaatgctccgtaacacccaatacgccggccgaaacttttttacaactctcctatgagtcgtttacccagaatgcacaggtacacttgtttagaggtaatccttcttt BBa_K1021015_sequence 1 tcgacagaagatgatattgaaggagcactttttgggcttggctggagctagtggaggtcaacaatgaatgcctattttggtttagtcgtccaggcggtgagcacaaaatttgtgtcgtttgacaagatggttcatttaggcaactggtcagatcagccccacttgtagcagtagcggcggcgctcgaagtgtgactcttattagcagacaggaacgaggacattattatcatctgctgcttggtgcacgataacttggtgcgtttgtcaagcaaggtaagtgaacgacccggtcataccttcttaagttcgcccttcctccctttatttcagattcaatctgacttacctattctacccaagcatcgattactagatgattgaacaagatggattgcacgcaggttctccggccgcttgggtggagaggctattcggctatgactgggcacaacagacaatcggctgctctgatgccgccgtgttccggctgtcagcgcaggggcgcccggttctttttgtcaagaccgacctgtccggtgccctgaatgaactccaggacgaggcagcgcggctatcgtggctggccacgacgggcgttccttgcgcagctgtgctcgacgttgtcactgaagcgggaagggactggctgctattgggcgaagtgccggggcaggatctcctgtcatctcaccttgctcctgccgagaaagtatccatcatggctgatgcaatgcggcggctgcatacgcttgatccggctacctgcccattcgaccaccaagcgaaacatcgcatcgagcgagcacgtactcggatggaagccggtcttgtcgatcaggatgatctggacgaagagcatcaggggctcgcgccagccgaactgttcgccaggctcaaggcgcgcatgcccgacggcgaggatctcgtcgtgacccatggcgatgcctgcttgccgaatatcatggtggaaaatggccgcttttctggattcatcgactgtggccggctgggtgtggcggaccgctatcaggacatagcgttggctacccgtgatattgctgaagagcttggcggcgaatgggctgaccgcttcctcgtgctttacggtatcgccgctcccgattcgcagcgcatcgccttctatcgccttcttgacgagttcttctgaggatcc BBa_K1021001_sequence 1 atgattgaacaagatggattgcacgcaggttctccggccgcttgggtggagaggctattcggctatgactgggcacaacagacaatcggctgctctgatgccgccgtgttccggctgtcagcgcaggggcgcccggttctttttgtcaagaccgacctgtccggtgccctgaatgaactccaggacgaggcagcgcggctatcgtggctggccacgacgggcgttccttgcgcagctgtgctcgacgttgtcactgaagcgggaagggactggctgctattgggcgaagtgccggggcaggatctcctgtcatctcaccttgctcctgccgagaaagtatccatcatggctgatgcaatgcggcggctgcatacgcttgatccggctacctgcccattcgaccaccaagcgaaacatcgcatcgagcgagcacgtactcggatggaagccggtcttgtcgatcaggatgatctggacgaagagcatcaggggctcgcgccagccgaactgttcgccaggctcaaggcgcgcatgcccgacggcgaggatctcgtcgtgacccatggcgatgcctgcttgccgaatatcatggtggaaaatggccgcttttctggattcatcgactgtggccggctgggtgtggcggaccgctatcaggacatagcgttggctacccgtgatattgctgaagagcttggcggcgaatgggctgaccgcttcctcgtgctttacggtatcgccgctcccgattcgcagcgcatcgccttctatcgccttcttgacgagttcttctgaggatcc BBa_K1021007_sequence 1 tcgacagaagatgatattgaaggagcactttttgggcttggctggagctagtggaggtcaacaatgaatgcctattttggtttagtcgtccaggcggtgagcacaaaatttgtgtcgtttgacaagatggttcatttaggcaactggtcagatcagccccacttgtagcagtagcggcggcgctcgaagtgtgactcttattagcagacaggaacgaggacattattatcatctgctgcttggtgcacgataacttggtgcgtttgtcaagcaaggtaagtgaacgacccggtcataccttcttaagttcgcccttcctccctttatttcagattcaatctgacttacctattctacccaagcatcgat igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z