BBa_K102132
1
BBa_K102132
TA13In anti-sense input in test harness
2008-10-27T12:00:00Z
2015-05-08T01:08:44Z
Synthetic
The input test harness provides a means to input one or more anti-sense RNA input signals to a T/AasL circuit. The test harness consists of an Arabinose cassette (AraC- PBad) which produces the anti-sense RNA input transcripts. Each input signal is flanked by a pair of hammerhead ribozymes to cleave the actual anti-sense signal from the rest of the transcript so that it may freely interact with (disrupt) its target gate.
false
false
_181_
0
2647
9
It's complicated
false
See Team wiki (2008 Alberta_NINT) Project page for description of algorithm
false
Wayne Materi
component1992248
1
BBa_I0500
component1992252
1
BBa_K102961
annotation1992252
1
BBa_K102961
range1992252
1
1219
1389
annotation1992248
1
BBa_I0500
range1992248
1
1
1210
BBa_I0500
1
pBad/araC
Inducible pBad/araC promoter
2003-12-05T12:00:00Z
2015-08-31T04:07:29Z
-- No description --
false
true
_1_
0
24
7
In stock
false
true
Sri Kosuri
annotation1498842
1
PC
range1498842
1
1043
1114
annotation1498841
1
Pbad
range1498841
1
1080
1210
annotation1498843
1
AraC
range1498843
1
1
1079
BBa_K102961
1
BBa_K102961
TA13In anti-sense input to BBa_K102911
2008-10-25T11:00:00Z
2015-05-08T01:08:46Z
synthetic
This provides the anti-sense input to disrupt (and thereby activate) the TA13 gate (BBa_K102911). The actual transcript includes two flanking hammerhead ribozymes (Taira et al, 1991) which self-cleave to release the anti-sense RNA.
false
false
_181_
0
2647
9
Not in stock
true
See Team wiki (2008 Alberta_NINT) Project page for description of algorithm
false
Wayne Materi
annotation1990333
1
hammerhead ribozyme 1
range1990333
1
14
75
annotation1990335
1
hammerhead ribozyme 2
range1990335
1
111
171
annotation1990334
1
TA13In anti-sense RNA
range1990334
1
77
106
BBa_K102961_sequence
1
ggagggatcattgtcgagctctgatgagtccgtgaggacgaaacggtagacagtaccgtcagctcgacaagctttcgaaccaatgcgacgcgacgaaggtggcgtaactactcgagatccgtcgaccagtcatgcctggtcctgatgagtccgtgaggacgaaacggatca
BBa_I0500_sequence
1
ttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaccccgtattggcaaatattgacggccagttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgaccgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcaaacaaattctcgtccctgatttttcaccaccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataaccgttggcctcaatcggcgttaaacccgccaccagatgggcattaaacgagtatcccggcagcaggggatcattttgcgcttcagccatacttttcatactcccgccattcagagaagaaaccaattgtccatattgcatcagacattgccgtcactgcgtcttttactggctcttctcgctaaccaaaccggtaaccccgcttattaaaagcattctgtaacaaagcgggaccaaagccatgacaaaaacgcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccatacccgtttttttgggctagc
BBa_K102132_sequence
1
ttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaccccgtattggcaaatattgacggccagttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgaccgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcaaacaaattctcgtccctgatttttcaccaccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataaccgttggcctcaatcggcgttaaacccgccaccagatgggcattaaacgagtatcccggcagcaggggatcattttgcgcttcagccatacttttcatactcccgccattcagagaagaaaccaattgtccatattgcatcagacattgccgtcactgcgtcttttactggctcttctcgctaaccaaaccggtaaccccgcttattaaaagcattctgtaacaaagcgggaccaaagccatgacaaaaacgcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccatacccgtttttttgggctagctactagagggagggatcattgtcgagctctgatgagtccgtgaggacgaaacggtagacagtaccgtcagctcgacaagctttcgaaccaatgcgacgcgacgaaggtggcgtaactactcgagatccgtcgaccagtcatgcctggtcctgatgagtccgtgaggacgaaacggatca
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z