LOCUS BBa_K1022100 3183 bp DNA linear 22-Nov-2024 DEFINITION pBAD:AIP receiver:GFP:TT ACCESSION BBa_K1022100 VERSION BBa_K1022100.1 FEATURES Location/Qualifiers promoter 1..131 /label=promoter misc_feature 40..57 /label=AraI1 misc_feature 61..78 /label=AraI2 promoter 1..130 /label=BBa_K206000 RBS 139..150 /label=B0034-sequence RBS RBS 1411..1422 /label=B0034-sequence RBS misc_feature 1405..1410 /label=BamHI site CDS 157..1404 /label=agrC coding sequence CDS 1429..2058 /label=agrA coding sequence misc_feature 139..2058 /label=BBa_I746100 stem_loop 2078..2121 /label=stem_loop misc_feature 2067..2146 /label=BBa_B0010 terminator 2067..2146 /label=BBa_B0010 stem_loop 2162..2181 /label=T7 TE misc_feature 2188..2188 /label=stop polyA_site 2182..2195 /label=polya misc_feature 2155..2195 /label=BBa_B0012 terminator 2155..2195 /label=BBa_B0012 promoter 2204..2299 /label=BBa_I746104 misc_feature 139..2299 /label=BBa_I746103 RBS 2308..2320 /label=RBS-3\Weak RBS 2314..2317 /label=RBS misc_feature 2308..2320 /label=BBa_B0032 RBS 2308..2320 /label=BBa_B0032 CDS 2327..3046 /label=GFP protein CDS 2327..3046 /label=BBa_E0040 stem_loop 3066..3109 /label=stem_loop misc_feature 3055..3134 /label=BBa_B0010 terminator 3055..3134 /label=BBa_B0010 stem_loop 3150..3169 /label=T7 TE misc_feature 3176..3176 /label=stop polyA_site 3170..3183 /label=polya misc_feature 3143..3183 /label=BBa_B0012 terminator 3143..3183 /label=BBa_B0012 misc_feature 2308..3183 /label=BBa_E0240 ORIGIN 1 acattgatta tttgcacggc gtcacacttt gctatgccat agcaagatag tccataagat 61 tagcggatcc tacctgacgc tttttatcgc aactctctac tgtttctcca taccgttttt 121 ttgggctagc tactagagaa agaggagaaa tactagatga ttctgatgtt caccattcca 181 gcaattatta gcggtattaa gtacagcaag ctggattatt tcttcatcat cgtaatctct 241 accctgtccc tgttcctgtt caaaatgttc gattccgcga gcctgattat cctgacttcc 301 ttcatcatca tcatgtattt cgtaaaaatc aaatggtata gcatcctgct gatcatgacc 361 tcccagatta tcctgtactg tgccaactac atgtatatcg ttatctacgc gtacatcacg 421 aaaatctccg attctatctt cgttatcttt ccgtctttct tcgtggtgta cgttacgatc 481 tctattctgt tttcctatat tatcaatcgc gttctgaaaa agatctctac tccgtacctg 541 attctgaaca aaggttttct gatcgtcatc tccactatcc tgctgctgac cttctctctg 601 ttcttctttt actctcagat caactccgat gaggcaaaag tcatccgtca gtacagcttc 661 atctttatcg gtattactat cttcctgagc attctgactt tcgttatcag ccagttcctg 721 ctgaaagaaa tgaagtacaa gcgcaaccaa gaagaaatcg agacttacta cgagtacacc 781 ctgaagattg aagcgatcaa caacgaaatg cgtaaatttc gtcacgacta cgttaacatt 841 ctgaccaccc tgagcgaata catccgtgaa gacgacatgc ctggcctgcg tgattatttc 901 aacaaaaaca ttgtgccgat gaaagataac ctgcaaatga acgctattaa gctgaacggt 961 atcgaaaacc tgaaagtacg tgagatcaag ggtctgatta ccgctaaaat cctgcgtgcg 1021 caggaaatga acatcccaat tagcatcgaa atcccagacg aagtttctag cattaacctg 1081 aacatgattg acctgtctcg tagcatcggc atcatcctgg acaacgctat cgaagcttcc 1141 actgaaatcg acgacccgat tattcgcgtt gcttttatcg agagcgaaaa ctccgtgacc 1201 tttatcgtga tgaacaaatg cgcggacgat atcccgcgta ttcacgaact gttccaggaa 1261 tctttcagca ccaagggtga aggtcgcggc ctgggcctgt ccaccctgaa agagatcgcg 1321 gataacgccg acaacgttct gctggacact attatcgaaa acggtttctt cattcagaaa 1381 gtcgaaatca tcaataacta ataaggatcc aaagaggaga aatactagat ggaaatcgca 1441 ctggctacgg acaatccata tgaggttctg gaacaggcta aaaacatgaa cgacatcggc 1501 tgctatttcc tggatattca gctgagcacc gacatcaacg gtatcaaact gggctctgaa 1561 atccgtaaac atgatccggt tggtaacatt attttcgtga cttctcattc tgagctgacc 1621 tatctgacgt tcgtatacaa ggttgcagcc atggatttca tctttaaaga cgatccggca 1681 gagctgcgta cgcgcatcat cgactgtctg gaaactgcgc acacgcgcct gcaactgctg 1741 tccaaagata actccgtgga aaccattgaa ctgaaacgtg gcagcaacag cgtttacgtt 1801 cagtacgatg atatcatgtt cttcgaaagc tccactaagt cccaccgtct gatcgcgcat 1861 ctggataatc gtcagatcga gttctatggc aacctgaaag agctgagcca gctggacgat 1921 cgcttcttcc gttgtcataa ctccttcgtt gttaaccgtc acaacatcga atccatcgac 1981 tccaaagagc gtattgtata cttcaagaac aaagaacact gttatgcgtc tgtacgcaat 2041 gtcaagaaaa tctaataata ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa 2101 agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctctact agagtcacac 2161 tggctcacct tcgggtgggc ctttctgcgt ttatatacta gagattaaat acaaattaca 2221 tttaacagtt aagtatttat ttcctacagt taggcaatat aatgataaaa gattgtacta 2281 aatcgtataa tgacagtgat actagagtca cacaggaaag tactagatgc gtaaaggaga 2341 agaacttttc actggagttg tcccaattct tgttgaatta gatggtgatg ttaatgggca 2401 caaattttct gtcagtggag agggtgaagg tgatgcaaca tacggaaaac ttacccttaa 2461 atttatttgc actactggaa aactacctgt tccatggcca acacttgtca ctactttcgg 2521 ttatggtgtt caatgctttg cgagataccc agatcatatg aaacagcatg actttttcaa 2581 gagtgccatg cccgaaggtt atgtacagga aagaactata tttttcaaag atgacgggaa 2641 ctacaagaca cgtgctgaag tcaagtttga aggtgatacc cttgttaata gaatcgagtt 2701 aaaaggtatt gattttaaag aagatggaaa cattcttgga cacaaattgg aatacaacta 2761 taactcacac aatgtataca tcatggcaga caaacaaaag aatggaatca aagttaactt 2821 caaaattaga cacaacattg aagatggaag cgttcaacta gcagaccatt atcaacaaaa 2881 tactccaatt ggcgatggcc ctgtcctttt accagacaac cattacctgt ccacacaatc 2941 tgccctttcg aaagatccca acgaaaagag agaccacatg gtccttcttg agtttgtaac 3001 agctgctggg attacacatg gcatggatga actatacaaa taataatact agagccaggc 3061 atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt 3121 cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct ttctgcgttt 3181 ata //