##gff-version 3
##sequence-region BBa_K1022100 1 3183
BBa_K1022100	.	engineered_region	139	2299	.	+	0	ID=BBa_I746103;Name=BBa_I746103
BBa_K1022100	.	engineered_region	139	2058	.	+	0	ID=BBa_I746100;Name=BBa_I746100;Parent=BBa_I746103
BBa_K1022100	.	ribosome_entry_site	139	150	.	+	0	ID=annotation1951374;Name=B0034-sequence RBS;Parent=BBa_I746100
BBa_K1022100	.	CDS	157	1404	.	+	0	ID=annotation1951377;Name=agrC coding sequence;Parent=BBa_I746100
BBa_K1022100	.	ribosome_entry_site	1411	1422	.	+	0	ID=annotation1951375;Name=B0034-sequence RBS;Parent=BBa_I746100
BBa_K1022100	.	CDS	1429	2058	.	+	0	ID=annotation1951378;Name=agrA coding sequence;Parent=BBa_I746100
BBa_K1022100	.	sequence_feature	1405	1410	.	+	0	ID=annotation1951376;Name=BamHI site;Parent=BBa_I746100
BBa_K1022100	.	promoter	2204	2299	.	+	0	ID=BBa_I746104;Name=BBa_I746104;Parent=BBa_I746103
BBa_K1022100	.	terminator	2067	2146	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_I746103
BBa_K1022100	.	engineered_region	2067	2146	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1022100	.	stem_loop	2078	2121	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1022100	.	terminator	2155	2195	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_I746103
BBa_K1022100	.	stop_codon	2188	2188	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1022100	.	polyA_site	2182	2195	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1022100	.	stem_loop	2162	2181	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1022100	.	engineered_region	2155	2195	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1022100	.	promoter	1	130	.	+	0	ID=BBa_K206000;Name=BBa_K206000
BBa_K1022100	.	promoter	1	131	.	+	0	ID=annotation2049252;Name=promoter;Parent=BBa_K206000
BBa_K1022100	.	operator	61	78	.	+	0	ID=annotation2049254;Name=AraI2;Parent=BBa_K206000
BBa_K1022100	.	operator	40	57	.	+	0	ID=annotation2049253;Name=AraI1;Parent=BBa_K206000
BBa_K1022100	.	engineered_region	2308	3183	.	+	0	ID=BBa_E0240;Name=BBa_E0240
BBa_K1022100	.	terminator	3143	3183	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_E0240
BBa_K1022100	.	stop_codon	3176	3176	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1022100	.	polyA_site	3170	3183	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1022100	.	stem_loop	3150	3169	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1022100	.	engineered_region	3143	3183	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1022100	.	terminator	3055	3134	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_E0240
BBa_K1022100	.	engineered_region	3055	3134	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1022100	.	stem_loop	3066	3109	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1022100	.	ribosome_entry_site	2308	2320	.	+	0	ID=BBa_B0032;Name=BBa_B0032;Parent=BBa_E0240
BBa_K1022100	.	ribosome_entry_site	2308	2320	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_K1022100	.	engineered_region	2308	2320	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_K1022100	.	ribosome_entry_site	2314	2317	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_K1022100	.	CDS	2327	3046	.	+	0	ID=BBa_E0040;Name=BBa_E0040;Parent=BBa_E0240
BBa_K1022100	.	CDS	2327	3046	.	+	0	ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040
>BBa_K1022100
acattgattatttgcacggcgtcacactttgctatgccatagcaagatagtccataagattagcggatcctacctgacg
ctttttatcgcaactctctactgtttctccataccgtttttttgggctagctactagagaaagaggagaaatactagat
gattctgatgttcaccattccagcaattattagcggtattaagtacagcaagctggattatttcttcatcatcgtaatc
tctaccctgtccctgttcctgttcaaaatgttcgattccgcgagcctgattatcctgacttccttcatcatcatcatgt
atttcgtaaaaatcaaatggtatagcatcctgctgatcatgacctcccagattatcctgtactgtgccaactacatgta
tatcgttatctacgcgtacatcacgaaaatctccgattctatcttcgttatctttccgtctttcttcgtggtgtacgtt
acgatctctattctgttttcctatattatcaatcgcgttctgaaaaagatctctactccgtacctgattctgaacaaag
gttttctgatcgtcatctccactatcctgctgctgaccttctctctgttcttcttttactctcagatcaactccgatga
ggcaaaagtcatccgtcagtacagcttcatctttatcggtattactatcttcctgagcattctgactttcgttatcagc
cagttcctgctgaaagaaatgaagtacaagcgcaaccaagaagaaatcgagacttactacgagtacaccctgaagattg
aagcgatcaacaacgaaatgcgtaaatttcgtcacgactacgttaacattctgaccaccctgagcgaatacatccgtga
agacgacatgcctggcctgcgtgattatttcaacaaaaacattgtgccgatgaaagataacctgcaaatgaacgctatt
aagctgaacggtatcgaaaacctgaaagtacgtgagatcaagggtctgattaccgctaaaatcctgcgtgcgcaggaaa
tgaacatcccaattagcatcgaaatcccagacgaagtttctagcattaacctgaacatgattgacctgtctcgtagcat
cggcatcatcctggacaacgctatcgaagcttccactgaaatcgacgacccgattattcgcgttgcttttatcgagagc
gaaaactccgtgacctttatcgtgatgaacaaatgcgcggacgatatcccgcgtattcacgaactgttccaggaatctt
tcagcaccaagggtgaaggtcgcggcctgggcctgtccaccctgaaagagatcgcggataacgccgacaacgttctgct
ggacactattatcgaaaacggtttcttcattcagaaagtcgaaatcatcaataactaataaggatccaaagaggagaaa
tactagatggaaatcgcactggctacggacaatccatatgaggttctggaacaggctaaaaacatgaacgacatcggct
gctatttcctggatattcagctgagcaccgacatcaacggtatcaaactgggctctgaaatccgtaaacatgatccggt
tggtaacattattttcgtgacttctcattctgagctgacctatctgacgttcgtatacaaggttgcagccatggatttc
atctttaaagacgatccggcagagctgcgtacgcgcatcatcgactgtctggaaactgcgcacacgcgcctgcaactgc
tgtccaaagataactccgtggaaaccattgaactgaaacgtggcagcaacagcgtttacgttcagtacgatgatatcat
gttcttcgaaagctccactaagtcccaccgtctgatcgcgcatctggataatcgtcagatcgagttctatggcaacctg
aaagagctgagccagctggacgatcgcttcttccgttgtcataactccttcgttgttaaccgtcacaacatcgaatcca
tcgactccaaagagcgtattgtatacttcaagaacaaagaacactgttatgcgtctgtacgcaatgtcaagaaaatcta
ataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtc
ggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagattaaatac
aaattacatttaacagttaagtatttatttcctacagttaggcaatataatgataaaagattgtactaaatcgtataat
gacagtgatactagagtcacacaggaaagtactagatgcgtaaaggagaagaacttttcactggagttgtcccaattct
tgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaa
cttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtg
ttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtaca
ggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgtt
aatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataact
cacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaaga
tggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccat
tacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacag
ctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctc
agtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttc
gggtgggcctttctgcgtttata