BBa_K1022100
type: ComponentDefinition
persistent identity: BBa K1022100
display id: BBa_K1022100
version: 1
was generated by: iGEM to SBOL conversion
description: pBAD:AIP receiver:GFP:TT
created: 2013-08-19T11:00:00Z
modified: 2015-05-08T01:08:44Z
mutable provenance: E.coli
owned by: james
owned by: myers
mutable description: This plasmid contains code for pBAD promoter, AIP receiver Agr A and Agr C, GFP along with double terminator.
discontinued: false
dominant: false
experience: experience/Works
group u list: _1329_
m user id: 0
owner id: 15691
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: Sequence was cloned using BBa_E0240 and BBa_I746103 from iGEM parts registry.
star: false
creator: Maithili Krishnan
type: DnaRegion
role: SO:0000804
role: partType/Reporter
component: BBa_E0240
component: BBa_I746103
component: BBa_K206000
sequence annotation: BBa_K206000
sequence annotation: BBa_I746103
sequence annotation: BBa_E0240
sequence: https://synbiohub.org/public/igem/BBa_K1022100_sequence/1

BBa_E0240
type: Component
persistent identity: component2332910
display id: component2332910
version: 1
top level: BBa_K1022100
definition: GFP report
access: public

BBa_I746103
type: Component
persistent identity: component2332899
display id: component2332899
version: 1
top level: BBa_K1022100
definition: BBa_I746103
access: public

BBa_K206000
type: Component
persistent identity: component2332885
display id: component2332885
version: 1
top level: BBa_K1022100
definition: pBAD
access: public

BBa_K206000
type: SequenceAnnotation
persistent identity: annotation2332885
display id: annotation2332885
version: 1
top level: BBa_K1022100
location: https://synbiohub.org/public/igem/BBa_K1022100/annotation2332885/range2332885/1
component: BBa_K206000

https://synbiohub.org/public/igem/BBa_K1022100/annotation2332885/range2332885/1
type: Range
persistent identity: range2332885
display id: range2332885
version: 1
top level: BBa_K1022100
direction: direction/reverse
start: 1
end: 130
orientation: inline

BBa_I746103
type: SequenceAnnotation
persistent identity: annotation2332899
display id: annotation2332899
version: 1
top level: BBa_K1022100
location: https://synbiohub.org/public/igem/BBa_K1022100/annotation2332899/range2332899/1
component: BBa_I746103

https://synbiohub.org/public/igem/BBa_K1022100/annotation2332899/range2332899/1
type: Range
persistent identity: range2332899
display id: range2332899
version: 1
top level: BBa_K1022100
direction: direction/reverse
start: 139
end: 2299
orientation: inline

BBa_E0240
type: SequenceAnnotation
persistent identity: annotation2332910
display id: annotation2332910
version: 1
top level: BBa_K1022100
location: https://synbiohub.org/public/igem/BBa_K1022100/annotation2332910/range2332910/1
component: BBa_E0240

https://synbiohub.org/public/igem/BBa_K1022100/annotation2332910/range2332910/1
type: Range
persistent identity: range2332910
display id: range2332910
version: 1
top level: BBa_K1022100
direction: direction/reverse
start: 2308
end: 3183
orientation: inline

BBa_I746103
type: ComponentDefinition
persistent identity: BBa I746103
display id: BBa_I746103
version: 1
was generated by: iGEM to SBOL conversion
description: I746101 (AIP sensor infrastructure - agrC + agrA with RBSes + Terminator) + I746104 (Inducible P2)
created: 2007-10-22T11:00:00Z
modified: 2015-08-31T04:08:04Z
mutable provenance: The original sequences for agrC and agrA were taken from the S. aureus Sequencing Project (results for strain NCTC8325) at Oklahoma University (http://www.genome.ou.edu/staph.html). The sequences were then codon-optimised for E. coli using GeneDesigner from DNA2.0 (http://www.dna20.com), and then synthesised by that company.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: This part is the AIP sensor infrastructure ([http://partsregistry.org/wiki/index.php/Part:BBa_I746101 I746101]) from the agr system with an AIP-inducible promoter P2 ([http://partsregistry.org/wiki/index.php/Part:BBa_I746104 I746104]) downstream. A constitutive/inducible promoter may be added upstream of I746101, which will cause production of the components of the signal transduction pathway (AgrC and AgrA); when phosphorylated AgrA is present, the AIP-sensitive promoter (P2) is activated and PoPS is output. In an application, this PoPS output could feed into a downstream device. The original part was made in order to transfer the S. aureus oligopeptide-based quorum sensing system into a BioBrick-compatible signalling mechanism. In the natural system, the signalling oligopeptide (termed AIP) is made from AgrD by the membrane-located enzyme AgrB. It is then detected by the membrane-located AgrC, which phosphorylates AgrA which then has DNA-binding activity and upregulates transcription of the promoters termed P2 and P3 in the agr locus. There are four known variants of AIP with different molecular structures and cross-inhibitory activity; this BioBrick generates group I AIP. The agr P2 operon is an autocatalytic sensory transduction system in Staphylococcus aureus. P2 promoter regulates the synthesis of AgrB (a transmembrane protein) and AgrD (precursor of AIP autoinducing peptide). AIP binds to AgrC, a membrane binding receptor and it phosphorylates AgrA. The phosphorylated AgrA increases the activity of agr P2 promoter about 50-fold.
discontinued: false
dominant: false
experience: experience/None
group u list: _116_
m user id: 0
owner id: 2127
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: N/A
star: false
creator: Lovelace Soirez
type: DnaRegion
role: SO:0000804
role: partType/Signalling
component: BBa_B0010
component: BBa_I746100
component: BBa_I746104
component: BBa_B0012
sequence annotation: BBa_I746100
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_I746104
sequence: https://synbiohub.org/public/igem/BBa_I746103_sequence/1

BBa_B0010
type: Component
persistent identity: component1952641
display id: component1952641
version: 1
top level: BBa_I746103
definition: BBa_B0010
access: public

BBa_I746100
type: Component
persistent identity: component1952640
display id: component1952640
version: 1
top level: BBa_I746103
definition: BBa_I746100
access: public

BBa_I746104
type: Component
persistent identity: component1952647
display id: component1952647
version: 1
top level: BBa_I746103
definition: BBa_I746104
access: public

BBa_B0012
type: Component
persistent identity: component1952643
display id: component1952643
version: 1
top level: BBa_I746103
definition: BBa_B0012
access: public

BBa_I746100
type: SequenceAnnotation
persistent identity: annotation1952640
display id: annotation1952640
version: 1
top level: BBa_I746103
location: https://synbiohub.org/public/igem/BBa_I746103/annotation1952640/range1952640/1
component: BBa_I746100

https://synbiohub.org/public/igem/BBa_I746103/annotation1952640/range1952640/1
type: Range
persistent identity: range1952640
display id: range1952640
version: 1
top level: BBa_I746103
direction: direction/reverse
start: 1
end: 1920
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1952641
display id: annotation1952641
version: 1
top level: BBa_I746103
location: https://synbiohub.org/public/igem/BBa_I746103/annotation1952641/range1952641/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I746103/annotation1952641/range1952641/1
type: Range
persistent identity: range1952641
display id: range1952641
version: 1
top level: BBa_I746103
direction: direction/forward
start: 1929
end: 2008
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1952643
display id: annotation1952643
version: 1
top level: BBa_I746103
location: https://synbiohub.org/public/igem/BBa_I746103/annotation1952643/range1952643/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I746103/annotation1952643/range1952643/1
type: Range
persistent identity: range1952643
display id: range1952643
version: 1
top level: BBa_I746103
direction: direction/forward
start: 2017
end: 2057
orientation: inline

BBa_I746104
type: SequenceAnnotation
persistent identity: annotation1952647
display id: annotation1952647
version: 1
top level: BBa_I746103
location: https://synbiohub.org/public/igem/BBa_I746103/annotation1952647/range1952647/1
component: BBa_I746104

https://synbiohub.org/public/igem/BBa_I746103/annotation1952647/range1952647/1
type: Range
persistent identity: range1952647
display id: range1952647
version: 1
top level: BBa_I746103
direction: direction/reverse
start: 2066
end: 2161
orientation: inline

GFP report
type: ComponentDefinition
persistent identity: BBa E0240
display id: BBa_E0240
version: 1
was generated by: iGEM to SBOL conversion
description: GFP generator
created: 2004-10-17T11:00:00Z
modified: 2015-08-31T04:07:26Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: B0032.E0040.B0015
discontinued: false
dominant: true
experience: experience/Works
group u list: _11_1_
m user id: 0
owner id: 61
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Jennifer Braff
type: DnaRegion
role: SO:0000804
role: partType/Reporter
component: BBa_B0032
component: BBa_B0010
component: BBa_E0040
component: BBa_B0012
sequence annotation: BBa_B0032
sequence annotation: BBa_E0040
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_E0240_sequence/1

BBa_B0032
type: Component
persistent identity: component1249213
display id: component1249213
version: 1
top level: GFP report
definition: BBa_B0032
access: public

BBa_B0010
type: Component
persistent identity: component1249221
display id: component1249221
version: 1
top level: GFP report
definition: BBa_B0010
access: public

BBa_E0040
type: Component
persistent identity: component1249216
display id: component1249216
version: 1
top level: GFP report
definition: GFP
access: public

BBa_B0012
type: Component
persistent identity: component1249231
display id: component1249231
version: 1
top level: GFP report
definition: BBa_B0012
access: public

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation1249213
display id: annotation1249213
version: 1
top level: GFP report
location: https://synbiohub.org/public/igem/BBa_E0240/annotation1249213/range1249213/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_E0240/annotation1249213/range1249213/1
type: Range
persistent identity: range1249213
display id: range1249213
version: 1
top level: GFP report
direction: direction/forward
start: 1
end: 13
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation1249216
display id: annotation1249216
version: 1
top level: GFP report
location: https://synbiohub.org/public/igem/BBa_E0240/annotation1249216/range1249216/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_E0240/annotation1249216/range1249216/1
type: Range
persistent identity: range1249216
display id: range1249216
version: 1
top level: GFP report
direction: direction/forward
start: 20
end: 739
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1249221
display id: annotation1249221
version: 1
top level: GFP report
location: https://synbiohub.org/public/igem/BBa_E0240/annotation1249221/range1249221/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_E0240/annotation1249221/range1249221/1
type: Range
persistent identity: range1249221
display id: range1249221
version: 1
top level: GFP report
direction: direction/forward
start: 748
end: 827
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1249231
display id: annotation1249231
version: 1
top level: GFP report
location: https://synbiohub.org/public/igem/BBa_E0240/annotation1249231/range1249231/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_E0240/annotation1249231/range1249231/1
type: Range
persistent identity: range1249231
display id: range1249231
version: 1
top level: GFP report
direction: direction/forward
start: 836
end: 876
orientation: inline

pBAD
type: ComponentDefinition
persistent identity: BBa K206000
display id: BBa_K206000
version: 1
was generated by: iGEM to SBOL conversion
description: pBAD strong
created: 2009-10-13T11:00:00Z
modified: 2015-05-08T01:11:23Z
mutable provenance: The sequence was obtained by applying all mutations that upregulated AraC binding and subsequent promoter activity listed in reference [1].
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: pBAD is an E.coli promoter that is induced by L-arabinose. K206000 is a mutagenized pBAD promoter that is responsive to lower concentrations of arabinose than wild type (<partinfo>I13453</partinfo>) and, additionally, exhibits a higher maximum of protein expression as measured by coupling to a fluorescent reporter.
discontinued: false
dominant: false
experience: experience/Works
group u list: _307_
m user id: 0
owner id: 4172
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable notes: There were no special design considerations.
star: false
creator: Amelia Hardjasa
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: promoter
sequence annotation: AraI1
sequence annotation: AraI2
sequence: https://synbiohub.org/public/igem/BBa_K206000_sequence/1

promoter
type: SequenceAnnotation
persistent identity: annotation2049252
display id: annotation2049252
version: 1
top level: pBAD
location: https://synbiohub.org/public/igem/BBa_K206000/annotation2049252/range2049252/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_K206000/annotation2049252/range2049252/1
type: Range
persistent identity: range2049252
display id: range2049252
version: 1
top level: pBAD
direction: direction/forward
start: 1
end: 131
orientation: inline

AraI1
type: SequenceAnnotation
persistent identity: annotation2049253
display id: annotation2049253
version: 1
top level: pBAD
location: https://synbiohub.org/public/igem/BBa_K206000/annotation2049253/range2049253/1
role: SO:0000057
role: feature/operator

https://synbiohub.org/public/igem/BBa_K206000/annotation2049253/range2049253/1
type: Range
persistent identity: range2049253
display id: range2049253
version: 1
top level: pBAD
direction: direction/forward
start: 40
end: 57
orientation: inline

AraI2
type: SequenceAnnotation
persistent identity: annotation2049254
display id: annotation2049254
version: 1
top level: pBAD
location: https://synbiohub.org/public/igem/BBa_K206000/annotation2049254/range2049254/1
role: SO:0000057
role: feature/operator

https://synbiohub.org/public/igem/BBa_K206000/annotation2049254/range2049254/1
type: Range
persistent identity: range2049254
display id: range2049254
version: 1
top level: pBAD
direction: direction/forward
start: 61
end: 78
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0032
type: ComponentDefinition
persistent identity: BBa B0032
display id: BBa_B0032
version: 1
was generated by: iGEM to SBOL conversion
description: RBS.3 (medium) -- derivative of BBa_0030
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Weak1 RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0030</bb_part>, <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0033</bb_part>.
discontinued: false
dominant: true
experience: experience/Works
group u list: _41_44_48_46_1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("RBS-2" in figure 4-14 of thesis). Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: partType/RBS
role: SO:0000139
sequence annotation: RBS-3\Weak
sequence annotation: BBa_B0032
sequence annotation: RBS
sequence: https://synbiohub.org/public/igem/BBa_B0032_sequence/1

RBS-3\Weak
type: SequenceAnnotation
persistent identity: annotation1709
display id: annotation1709
version: 1
top level: BBa_B0032
location: https://synbiohub.org/public/igem/BBa_B0032/annotation1709/range1709/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_B0032/annotation1709/range1709/1
type: Range
persistent identity: range1709
display id: range1709
version: 1
top level: BBa_B0032
direction: direction/forward
start: 1
end: 13
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation7027
display id: annotation7027
version: 1
top level: BBa_B0032
location: https://synbiohub.org/public/igem/BBa_B0032/annotation7027/range7027/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0032/annotation7027/range7027/1
type: Range
persistent identity: range7027
display id: range7027
version: 1
top level: BBa_B0032
direction: direction/forward
start: 1
end: 13
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation1710
display id: annotation1710
version: 1
top level: BBa_B0032
location: https://synbiohub.org/public/igem/BBa_B0032/annotation1710/range1710/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_B0032/annotation1710/range1710/1
type: Range
persistent identity: range1710
display id: range1710
version: 1
top level: BBa_B0032
direction: direction/forward
start: 7
end: 10
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

GFP
type: ComponentDefinition
persistent identity: BBa E0040
display id: BBa_E0040
version: 1
was generated by: iGEM to SBOL conversion
description: green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (SwissProt: P42212
created: 2004-09-29T11:00:00Z
modified: 2016-01-26T02:09:38Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: GFP (mut3b) [note that this part does not have a barcode]
discontinued: false
dominant: true
experience: experience/Works
group u list: _11_1_
m user id: 4206
owner id: 61
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: jcbraff
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: GFP protein
sequence: https://synbiohub.org/public/igem/BBa_E0040_sequence/1

GFP protein
type: SequenceAnnotation
persistent identity: annotation1934520
display id: annotation1934520
version: 1
top level: GFP
location: https://synbiohub.org/public/igem/BBa_E0040/annotation1934520/range1934520/1
role: SO:0000316
role: feature/protein

https://synbiohub.org/public/igem/BBa_E0040/annotation1934520/range1934520/1
type: Range
persistent identity: range1934520
display id: range1934520
version: 1
top level: GFP
direction: direction/forward
start: 1
end: 720
orientation: inline

BBa_I746100
type: ComponentDefinition
persistent identity: BBa I746100
display id: BBa_I746100
version: 1
was generated by: iGEM to SBOL conversion
description: AIP sensor infrastructure (agrC + agrA from S. aureus with RBSes)
created: 2007-10-19T11:00:00Z
modified: 2015-08-31T04:08:04Z
mutable provenance: The original sequences for agrC and agrA were taken from the ''S. aureus'' Sequencing Project (results for strain NCTC8325) at Oklahoma University (http://www.genome.ou.edu/staph.html). The sequences were then codon-optimised for ''E. coli'' using GeneDesigner from DNA2.0 (http://www.dna20.com), and then synthesised by that company.
owned by: james
owned by: myers
mutable description: This part was made in order to transfer the S. aureus oligopeptide-based quorum sensing system into a BioBrick-compatible signalling mechanism. In the natural system, the signalling oligopeptide (termed AIP) is made from AgrD by the membrane-located enzyme AgrB. It is then detected by the membrane-located AgrC, which phosphorylates AgrA which then has DNA-binding activity and upregulates transcription of the promoters termed P2 and P3 in the agr locus. There are four known variants of AIP with different molecular structures and cross-inhibitory activity; this BioBrick generates group I AIP. This part contains the coding regions for group I agrC and agrA (in that order) from ''S. aureus'' strain NCTC8325, prefixed by the B0034 RBS and separated by a BamHI site.
discontinued: false
dominant: false
group u list: _116_
m user id: 0
owner id: 1851
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: N/A
star: false
creator: David Wyatt
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence annotation: B0034-sequence RBS
sequence annotation: agrC coding sequence
sequence annotation: BamHI site
sequence annotation: agrA coding sequence
sequence: https://synbiohub.org/public/igem/BBa_I746100_sequence/1

B0034-sequence RBS
type: SequenceAnnotation
persistent identity: annotation1951374
display id: annotation1951374
version: 1
top level: BBa_I746100
location: https://synbiohub.org/public/igem/BBa_I746100/annotation1951374/range1951374/1
role: SO:0000139
role: feature/rbs

https://synbiohub.org/public/igem/BBa_I746100/annotation1951374/range1951374/1
type: Range
persistent identity: range1951374
display id: range1951374
version: 1
top level: BBa_I746100
direction: direction/forward
start: 1
end: 12
orientation: inline

agrC coding sequence
type: SequenceAnnotation
persistent identity: annotation1951377
display id: annotation1951377
version: 1
top level: BBa_I746100
location: https://synbiohub.org/public/igem/BBa_I746100/annotation1951377/range1951377/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_I746100/annotation1951377/range1951377/1
type: Range
persistent identity: range1951377
display id: range1951377
version: 1
top level: BBa_I746100
direction: direction/forward
start: 19
end: 1266
orientation: inline

BamHI site
type: SequenceAnnotation
persistent identity: annotation1951376
display id: annotation1951376
version: 1
top level: BBa_I746100
location: https://synbiohub.org/public/igem/BBa_I746100/annotation1951376/range1951376/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_I746100/annotation1951376/range1951376/1
type: Range
persistent identity: range1951376
display id: range1951376
version: 1
top level: BBa_I746100
direction: direction/forward
start: 1267
end: 1272
orientation: inline

B0034-sequence RBS
type: SequenceAnnotation
persistent identity: annotation1951375
display id: annotation1951375
version: 1
top level: BBa_I746100
location: https://synbiohub.org/public/igem/BBa_I746100/annotation1951375/range1951375/1
role: SO:0000139
role: feature/rbs

https://synbiohub.org/public/igem/BBa_I746100/annotation1951375/range1951375/1
type: Range
persistent identity: range1951375
display id: range1951375
version: 1
top level: BBa_I746100
direction: direction/forward
start: 1273
end: 1284
orientation: inline

agrA coding sequence
type: SequenceAnnotation
persistent identity: annotation1951378
display id: annotation1951378
version: 1
top level: BBa_I746100
location: https://synbiohub.org/public/igem/BBa_I746100/annotation1951378/range1951378/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_I746100/annotation1951378/range1951378/1
type: Range
persistent identity: range1951378
display id: range1951378
version: 1
top level: BBa_I746100
direction: direction/forward
start: 1291
end: 1920
orientation: inline

BBa_I746104
type: ComponentDefinition
persistent identity: BBa I746104
display id: BBa_I746104
version: 1
was generated by: iGEM to SBOL conversion
description: P2 promoter in agr operon from S. aureus
created: 2007-08-30T11:00:00Z
modified: 2015-08-31T04:08:04Z
mutable provenance: The section of the sequence constituting the P2 promoter is subject to rather more debate than the sequences of the coding regions. [http://jb.asm.org/cgi/content/full/186/22/7549 Koenig, Robbin L., Ray, Jessica L., Maleki, Soheila J., Smeltzer, Mark S., Hurlburt, Barry K. Staphylococcus aureus AgrA Binding to the RNAIII-agr Regulatory Region J. Bacteriol. 2004 186: 7549-7555] shows the areas of the sequence to which phosphorylated AgrA binds while L Rao, R K Karls, and M J Betley: In vitro transcription of pathogenesis-related genes by purified RNA polymerase from Staphylococcus aureus. Bacteriol. 1995 May; 177(10): 2609???2614. shows the transcription start sites; we took the overall extent of the promoter region from that labelled in Morfeldt, E., K. Tegmark, and S. Arvidson. 1996. Transcriptional control of the agr-dependent virulence gene regulator, RNAIII, in Staphylococcus aureus. Mol. Microbiol. 21:1227???1237. (not available online).
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: The agr P2 operon is an autocatalytic sensory transduction system in Staphylococcus aureus. P2 promoter regulates the synthesis of AgrB (a transmembrane protein) and AgrD (precursor of AIP autoinducing peptide). AIP binds to AgrC, a membrane binding receptor and it phosphorylates AgrA. The phosphorylated AgrA increases the expression level from P2 promoter.
discontinued: false
dominant: false
experience: experience/None
group u list: _116_
m user id: 0
owner id: 2121
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Codon usage has been optimised for E. coli (which also seems to give good results for B. subtilis
star: true
creator: Zhizhen Zhao
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I746104_sequence/1

https://synbiohub.org/public/igem/BBa_K1022100_sequence/1
type: Sequence
persistent identity: BBa K1022100 sequence
display id: BBa_K1022100_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: acattgattatttgcacggcgtcacactttgctatgccatagcaagatagtccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccataccgtttttttgggctagctactagagaaagaggagaaatactagatgattctgatgttcaccattccagcaattattagcggtattaagtacagcaagctggattatttcttcatcatcgtaatctctaccctgtccctgttcctgttcaaaatgttcgattccgcgagcctgattatcctgacttccttcatcatcatcatgtatttcgtaaaaatcaaatggtatagcatcctgctgatcatgacctcccagattatcctgtactgtgccaactacatgtatatcgttatctacgcgtacatcacgaaaatctccgattctatcttcgttatctttccgtctttcttcgtggtgtacgttacgatctctattctgttttcctatattatcaatcgcgttctgaaaaagatctctactccgtacctgattctgaacaaaggttttctgatcgtcatctccactatcctgctgctgaccttctctctgttcttcttttactctcagatcaactccgatgaggcaaaagtcatccgtcagtacagcttcatctttatcggtattactatcttcctgagcattctgactttcgttatcagccagttcctgctgaaagaaatgaagtacaagcgcaaccaagaagaaatcgagacttactacgagtacaccctgaagattgaagcgatcaacaacgaaatgcgtaaatttcgtcacgactacgttaacattctgaccaccctgagcgaatacatccgtgaagacgacatgcctggcctgcgtgattatttcaacaaaaacattgtgccgatgaaagataacctgcaaatgaacgctattaagctgaacggtatcgaaaacctgaaagtacgtgagatcaagggtctgattaccgctaaaatcctgcgtgcgcaggaaatgaacatcccaattagcatcgaaatcccagacgaagtttctagcattaacctgaacatgattgacctgtctcgtagcatcggcatcatcctggacaacgctatcgaagcttccactgaaatcgacgacccgattattcgcgttgcttttatcgagagcgaaaactccgtgacctttatcgtgatgaacaaatgcgcggacgatatcccgcgtattcacgaactgttccaggaatctttcagcaccaagggtgaaggtcgcggcctgggcctgtccaccctgaaagagatcgcggataacgccgacaacgttctgctggacactattatcgaaaacggtttcttcattcagaaagtcgaaatcatcaataactaataaggatccaaagaggagaaatactagatggaaatcgcactggctacggacaatccatatgaggttctggaacaggctaaaaacatgaacgacatcggctgctatttcctggatattcagctgagcaccgacatcaacggtatcaaactgggctctgaaatccgtaaacatgatccggttggtaacattattttcgtgacttctcattctgagctgacctatctgacgttcgtatacaaggttgcagccatggatttcatctttaaagacgatccggcagagctgcgtacgcgcatcatcgactgtctggaaactgcgcacacgcgcctgcaactgctgtccaaagataactccgtggaaaccattgaactgaaacgtggcagcaacagcgtttacgttcagtacgatgatatcatgttcttcgaaagctccactaagtcccaccgtctgatcgcgcatctggataatcgtcagatcgagttctatggcaacctgaaagagctgagccagctggacgatcgcttcttccgttgtcataactccttcgttgttaaccgtcacaacatcgaatccatcgactccaaagagcgtattgtata…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I746103_sequence/1
type: Sequence
persistent identity: BBa I746103 sequence
display id: BBa_I746103_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatgattctgatgttcaccattccagcaattattagcggtattaagtacagcaagctggattatttcttcatcatcgtaatctctaccctgtccctgttcctgttcaaaatgttcgattccgcgagcctgattatcctgacttccttcatcatcatcatgtatttcgtaaaaatcaaatggtatagcatcctgctgatcatgacctcccagattatcctgtactgtgccaactacatgtatatcgttatctacgcgtacatcacgaaaatctccgattctatcttcgttatctttccgtctttcttcgtggtgtacgttacgatctctattctgttttcctatattatcaatcgcgttctgaaaaagatctctactccgtacctgattctgaacaaaggttttctgatcgtcatctccactatcctgctgctgaccttctctctgttcttcttttactctcagatcaactccgatgaggcaaaagtcatccgtcagtacagcttcatctttatcggtattactatcttcctgagcattctgactttcgttatcagccagttcctgctgaaagaaatgaagtacaagcgcaaccaagaagaaatcgagacttactacgagtacaccctgaagattgaagcgatcaacaacgaaatgcgtaaatttcgtcacgactacgttaacattctgaccaccctgagcgaatacatccgtgaagacgacatgcctggcctgcgtgattatttcaacaaaaacattgtgccgatgaaagataacctgcaaatgaacgctattaagctgaacggtatcgaaaacctgaaagtacgtgagatcaagggtctgattaccgctaaaatcctgcgtgcgcaggaaatgaacatcccaattagcatcgaaatcccagacgaagtttctagcattaacctgaacatgattgacctgtctcgtagcatcggcatcatcctggacaacgctatcgaagcttccactgaaatcgacgacccgattattcgcgttgcttttatcgagagcgaaaactccgtgacctttatcgtgatgaacaaatgcgcggacgatatcccgcgtattcacgaactgttccaggaatctttcagcaccaagggtgaaggtcgcggcctgggcctgtccaccctgaaagagatcgcggataacgccgacaacgttctgctggacactattatcgaaaacggtttcttcattcagaaagtcgaaatcatcaataactaataaggatccaaagaggagaaatactagatggaaatcgcactggctacggacaatccatatgaggttctggaacaggctaaaaacatgaacgacatcggctgctatttcctggatattcagctgagcaccgacatcaacggtatcaaactgggctctgaaatccgtaaacatgatccggttggtaacattattttcgtgacttctcattctgagctgacctatctgacgttcgtatacaaggttgcagccatggatttcatctttaaagacgatccggcagagctgcgtacgcgcatcatcgactgtctggaaactgcgcacacgcgcctgcaactgctgtccaaagataactccgtggaaaccattgaactgaaacgtggcagcaacagcgtttacgttcagtacgatgatatcatgttcttcgaaagctccactaagtcccaccgtctgatcgcgcatctggataatcgtcagatcgagttctatggcaacctgaaagagctgagccagctggacgatcgcttcttccgttgtcataactccttcgttgttaaccgtcacaacatcgaatccatcgactccaaagagcgtattgtatacttcaagaacaaagaacactgttatgcgtctgtacgcaatgtcaagaaaatctaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtga…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E0240_sequence/1
type: Sequence
persistent identity: BBa E0240 sequence
display id: BBa_E0240_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacacaggaaagtactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K206000_sequence/1
type: Sequence
persistent identity: BBa K206000 sequence
display id: BBa_K206000_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: acattgattatttgcacggcgtcacactttgctatgccatagcaagatagtccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccataccgtttttttgggctagc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0032_sequence/1
type: Sequence
persistent identity: BBa B0032 sequence
display id: BBa_B0032_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacacaggaaag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E0040_sequence/1
type: Sequence
persistent identity: BBa E0040 sequence
display id: BBa_E0040_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: myers
owned by: james
elements: atgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I746100_sequence/1
type: Sequence
persistent identity: BBa I746100 sequence
display id: BBa_I746100_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatgattctgatgttcaccattccagcaattattagcggtattaagtacagcaagctggattatttcttcatcatcgtaatctctaccctgtccctgttcctgttcaaaatgttcgattccgcgagcctgattatcctgacttccttcatcatcatcatgtatttcgtaaaaatcaaatggtatagcatcctgctgatcatgacctcccagattatcctgtactgtgccaactacatgtatatcgttatctacgcgtacatcacgaaaatctccgattctatcttcgttatctttccgtctttcttcgtggtgtacgttacgatctctattctgttttcctatattatcaatcgcgttctgaaaaagatctctactccgtacctgattctgaacaaaggttttctgatcgtcatctccactatcctgctgctgaccttctctctgttcttcttttactctcagatcaactccgatgaggcaaaagtcatccgtcagtacagcttcatctttatcggtattactatcttcctgagcattctgactttcgttatcagccagttcctgctgaaagaaatgaagtacaagcgcaaccaagaagaaatcgagacttactacgagtacaccctgaagattgaagcgatcaacaacgaaatgcgtaaatttcgtcacgactacgttaacattctgaccaccctgagcgaatacatccgtgaagacgacatgcctggcctgcgtgattatttcaacaaaaacattgtgccgatgaaagataacctgcaaatgaacgctattaagctgaacggtatcgaaaacctgaaagtacgtgagatcaagggtctgattaccgctaaaatcctgcgtgcgcaggaaatgaacatcccaattagcatcgaaatcccagacgaagtttctagcattaacctgaacatgattgacctgtctcgtagcatcggcatcatcctggacaacgctatcgaagcttccactgaaatcgacgacccgattattcgcgttgcttttatcgagagcgaaaactccgtgacctttatcgtgatgaacaaatgcgcggacgatatcccgcgtattcacgaactgttccaggaatctttcagcaccaagggtgaaggtcgcggcctgggcctgtccaccctgaaagagatcgcggataacgccgacaacgttctgctggacactattatcgaaaacggtttcttcattcagaaagtcgaaatcatcaataactaataaggatccaaagaggagaaatactagatggaaatcgcactggctacggacaatccatatgaggttctggaacaggctaaaaacatgaacgacatcggctgctatttcctggatattcagctgagcaccgacatcaacggtatcaaactgggctctgaaatccgtaaacatgatccggttggtaacattattttcgtgacttctcattctgagctgacctatctgacgttcgtatacaaggttgcagccatggatttcatctttaaagacgatccggcagagctgcgtacgcgcatcatcgactgtctggaaactgcgcacacgcgcctgcaactgctgtccaaagataactccgtggaaaccattgaactgaaacgtggcagcaacagcgtttacgttcagtacgatgatatcatgttcttcgaaagctccactaagtcccaccgtctgatcgcgcatctggataatcgtcagatcgagttctatggcaacctgaaagagctgagccagctggacgatcgcttcttccgttgtcataactccttcgttgttaaccgtcacaacatcgaatccatcgactccaaagagcgtattgtatacttcaagaacaaagaacactgttatgcgtctgtacgcaatgtcaagaaaatctaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I746104_sequence/1
type: Sequence
persistent identity: BBa I746104 sequence
display id: BBa_I746104_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: attaaatacaaattacatttaacagttaagtatttatttcctacagttaggcaatataatgataaaagattgtactaaatcgtataatgacagtga
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: Chris J. Myers
creator: James Alastair McLaughlin
ended at time: 2017-03-06T15:00:00.000Z