LOCUS       BBa_K1022104            1275 bp    DNA     linear       05-Nov-2024
DEFINITION  pT7:  RBS: His6- SUMO: MaximinH5: RBS TetR :TT
ACCESSION   BBa_K1022104
VERSION     BBa_K1022104.1  
FEATURES             Location/Qualifiers
     promoter        1..28
                     /label=T7
     RBS             29..42
                     /label=34
     misc_feature    43..363
                     /label=His6:SUMO
     misc_feature    364..427
                     /label=MaximinH5
     misc_feature    1..427
                     /label=BBa_K1022101
     misc_feature    440..443
                     /label=conserved
     RBS             436..447
                     /label=BBa_B0034
     CDS             457..1073
                     /label=tetR
     misc_feature    1074..1107
                     /label=SsrA
     misc_feature    1114..1138
                     /label=Help:Barcodes
     CDS             454..1113
                     /label=BBa_C0040
     stem_loop       1158..1201
                     /label=stem_loop
     misc_feature    1147..1226
                     /label=BBa_B0010
     terminator      1147..1226
                     /label=BBa_B0010
     stem_loop       1242..1261
                     /label=T7 TE
     misc_feature    1268..1268
                     /label=stop
     polyA_site      1262..1275
                     /label=polya
     misc_feature    1235..1275
                     /label=BBa_B0012
     terminator      1235..1275
                     /label=BBa_B0012
     misc_feature    436..1275
                     /label=BBa_P0440
ORIGIN
        1 taatacgact cactataggg agagaaagag gagaaatact agatggctag catgcatcat
       61 catcatcatc attcggactc agaagtcaat caagaagcta agccagaggt caagccagaa
      121 gtcaagcctg agactcacat caatttaaag gtgtccgatg gatcttcaga gatcttcttc
      181 aagatcaaaa agaccactcc tttaagaagg ctgatggaag cgttcgctaa acgtcagggt
      241 aaggaaatgg actccttaag attcttgtac gacggtatta ggattcaagc tgatcagacc
      301 cctgaagatt tggacatgga tgataacgat attattgagg ctcacagaga acagaccggt
      361 ggtatcctgg gtccggttct gggtctggtt tctgacaccc tggacgacgt tctgggtatc
      421 ctgtaattac tagagaaaga ggagaaatac tagatgtcca gattagataa aagtaaagtg
      481 attaacagcg cattagagct gcttaatgag gtcggaatcg aaggtttaac aacccgtaaa
      541 ctcgcccaga agctaggtgt agagcagcct acattgtatt ggcatgtaaa aaataagcgg
      601 gctttgctcg acgccttagc cattgagatg ttagataggc accatactca cttttgccct
      661 ttagaagggg aaagctggca agatttttta cgtaataacg ctaaaagttt tagatgtgct
      721 ttactaagtc atcgcgatgg agcaaaagta catttaggta cacggcctac agaaaaacag
      781 tatgaaactc tcgaaaatca attagccttt ttatgccaac aaggtttttc actagagaat
      841 gcattatatg cactcagcgc tgtggggcat tttactttag gttgcgtatt ggaagatcaa
      901 gagcatcaag tcgctaaaga agaaagggaa acacctacta ctgatagtat gccgccatta
      961 ttacgacaag ctatcgaatt atttgatcac caaggtgcag agccagcctt cttattcggc
     1021 cttgaattga tcatatgcgg attagaaaaa caacttaaat gtgaaagtgg gtccgctgca
     1081 aacgacgaaa actacgcttt agtagcttaa taacactgat agtgctagtg tagatcacta
     1141 ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta
     1201 tctgttgttt gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc
     1261 ctttctgcgt ttata
//