LOCUS       BBa_K1022105            1260 bp    DNA     linear       22-Nov-2024
DEFINITION  pT7: RBS: His6- SUMO: Signiferin: RBS TetR :TT
ACCESSION   BBa_K1022105
VERSION     BBa_K1022105.1  
FEATURES             Location/Qualifiers
     promoter        1..28
                     /label=T7
     RBS             29..42
                     /label=34
     misc_feature    43..363
                     /label=His6:SUMO
     misc_feature    364..412
                     /label=Signiferin
     misc_feature    1..412
                     /label=BBa_K1022102
     misc_feature    425..428
                     /label=conserved
     RBS             421..432
                     /label=BBa_B0034
     CDS             442..1058
                     /label=tetR
     misc_feature    1059..1092
                     /label=SsrA
     misc_feature    1099..1123
                     /label=Help:Barcodes
     CDS             439..1098
                     /label=BBa_C0040
     stem_loop       1143..1186
                     /label=stem_loop
     misc_feature    1132..1211
                     /label=BBa_B0010
     terminator      1132..1211
                     /label=BBa_B0010
     stem_loop       1227..1246
                     /label=T7 TE
     misc_feature    1253..1253
                     /label=stop
     polyA_site      1247..1260
                     /label=polya
     misc_feature    1220..1260
                     /label=BBa_B0012
     terminator      1220..1260
                     /label=BBa_B0012
     misc_feature    421..1260
                     /label=BBa_P0440
ORIGIN
        1 taatacgact cactataggg agagaaagag gagaaatact agatggctag catgcatcat
       61 catcatcatc attcggactc agaagtcaat caagaagcta agccagaggt caagccagaa
      121 gtcaagcctg agactcacat caatttaaag gtgtccgatg gatcttcaga gatcttcttc
      181 aagatcaaaa agaccactcc tttaagaagg ctgatggaag cgttcgctaa acgtcagggt
      241 aaggaaatgg actccttaag attcttgtac gacggtatta ggattcaagc tgatcagacc
      301 cctgaagatt tggacatgga tgataacgat attattgagg ctcacagaga acagaccggt
      361 ggtatcatcg gtcacctgat caaaaccgcg ctgggtatgc tgggtctgta attactagag
      421 aaagaggaga aatactagat gtccagatta gataaaagta aagtgattaa cagcgcatta
      481 gagctgctta atgaggtcgg aatcgaaggt ttaacaaccc gtaaactcgc ccagaagcta
      541 ggtgtagagc agcctacatt gtattggcat gtaaaaaata agcgggcttt gctcgacgcc
      601 ttagccattg agatgttaga taggcaccat actcactttt gccctttaga aggggaaagc
      661 tggcaagatt ttttacgtaa taacgctaaa agttttagat gtgctttact aagtcatcgc
      721 gatggagcaa aagtacattt aggtacacgg cctacagaaa aacagtatga aactctcgaa
      781 aatcaattag cctttttatg ccaacaaggt ttttcactag agaatgcatt atatgcactc
      841 agcgctgtgg ggcattttac tttaggttgc gtattggaag atcaagagca tcaagtcgct
      901 aaagaagaaa gggaaacacc tactactgat agtatgccgc cattattacg acaagctatc
      961 gaattatttg atcaccaagg tgcagagcca gccttcttat tcggccttga attgatcata
     1021 tgcggattag aaaaacaact taaatgtgaa agtgggtccg ctgcaaacga cgaaaactac
     1081 gctttagtag cttaataaca ctgatagtgc tagtgtagat cactactaga gccaggcatc
     1141 aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg
     1201 tgaacgctct ctactagagt cacactggct caccttcggg tgggcctttc tgcgtttata
//