LOCUS BBa_K1022105 1260 bp DNA linear 05-Nov-2024 DEFINITION pT7: RBS: His6- SUMO: Signiferin: RBS TetR :TT ACCESSION BBa_K1022105 VERSION BBa_K1022105.1 FEATURES Location/Qualifiers promoter 1..28 /label=T7 RBS 29..42 /label=34 misc_feature 43..363 /label=His6:SUMO misc_feature 364..412 /label=Signiferin misc_feature 1..412 /label=BBa_K1022102 misc_feature 425..428 /label=conserved RBS 421..432 /label=BBa_B0034 CDS 442..1058 /label=tetR misc_feature 1059..1092 /label=SsrA misc_feature 1099..1123 /label=Help:Barcodes CDS 439..1098 /label=BBa_C0040 stem_loop 1143..1186 /label=stem_loop misc_feature 1132..1211 /label=BBa_B0010 terminator 1132..1211 /label=BBa_B0010 stem_loop 1227..1246 /label=T7 TE misc_feature 1253..1253 /label=stop polyA_site 1247..1260 /label=polya misc_feature 1220..1260 /label=BBa_B0012 terminator 1220..1260 /label=BBa_B0012 misc_feature 421..1260 /label=BBa_P0440 ORIGIN 1 taatacgact cactataggg agagaaagag gagaaatact agatggctag catgcatcat 61 catcatcatc attcggactc agaagtcaat caagaagcta agccagaggt caagccagaa 121 gtcaagcctg agactcacat caatttaaag gtgtccgatg gatcttcaga gatcttcttc 181 aagatcaaaa agaccactcc tttaagaagg ctgatggaag cgttcgctaa acgtcagggt 241 aaggaaatgg actccttaag attcttgtac gacggtatta ggattcaagc tgatcagacc 301 cctgaagatt tggacatgga tgataacgat attattgagg ctcacagaga acagaccggt 361 ggtatcatcg gtcacctgat caaaaccgcg ctgggtatgc tgggtctgta attactagag 421 aaagaggaga aatactagat gtccagatta gataaaagta aagtgattaa cagcgcatta 481 gagctgctta atgaggtcgg aatcgaaggt ttaacaaccc gtaaactcgc ccagaagcta 541 ggtgtagagc agcctacatt gtattggcat gtaaaaaata agcgggcttt gctcgacgcc 601 ttagccattg agatgttaga taggcaccat actcactttt gccctttaga aggggaaagc 661 tggcaagatt ttttacgtaa taacgctaaa agttttagat gtgctttact aagtcatcgc 721 gatggagcaa aagtacattt aggtacacgg cctacagaaa aacagtatga aactctcgaa 781 aatcaattag cctttttatg ccaacaaggt ttttcactag agaatgcatt atatgcactc 841 agcgctgtgg ggcattttac tttaggttgc gtattggaag atcaagagca tcaagtcgct 901 aaagaagaaa gggaaacacc tactactgat agtatgccgc cattattacg acaagctatc 961 gaattatttg atcaccaagg tgcagagcca gccttcttat tcggccttga attgatcata 1021 tgcggattag aaaaacaact taaatgtgaa agtgggtccg ctgcaaacga cgaaaactac 1081 gctttagtag cttaataaca ctgatagtgc tagtgtagat cactactaga gccaggcatc 1141 aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg 1201 tgaacgctct ctactagagt cacactggct caccttcggg tgggcctttc tgcgtttata //