LOCUS BBa_K1022108 992 bp DNA linear 24-Nov-2024 DEFINITION pTet: cI: TT ACCESSION BBa_K1022108 VERSION BBa_K1022108.1 FEATURES Location/Qualifiers misc_feature 1..19 /label=TetR 1 misc_feature 1..54 /label=BBa_R0040 promoter 20..25 /label=-35 misc_feature 26..44 /label=TetR 2 promoter 43..48 /label=-10 promoter 1..54 /label=BBa_R0040 misc_feature 67..70 /label=conserved RBS 63..74 /label=BBa_B0034 promoter 1..74 /label=BBa_J13002 CDS 84..791 /label=cI lambda misc_feature 792..824 /label=LVA misc_feature 831..855 /label=Help:Barcodes CDS 81..855 /label=BBa_C0051 stem_loop 875..918 /label=stem_loop misc_feature 864..943 /label=BBa_B0010 terminator 864..943 /label=BBa_B0010 stem_loop 959..978 /label=T7 TE misc_feature 985..985 /label=stop polyA_site 979..992 /label=polya misc_feature 952..992 /label=BBa_B0012 terminator 952..992 /label=BBa_B0012 terminator 864..992 /label=BBa_B0015 ORIGIN 1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag 61 agaaagagga gaaatactag atgagcacaa aaaagaaacc attaacacaa gagcagcttg 121 aggacgcacg tcgccttaaa gcaatttatg aaaaaaagaa aaatgaactt ggcttatccc 181 aggaatctgt cgcagacaag atggggatgg ggcagtcagg cgttggtgct ttatttaatg 241 gcatcaatgc attaaatgct tataacgccg cattgcttgc aaaaattctc aaagttagcg 301 ttgaagaatt tagcccttca atcgccagag aaatctacga gatgtatgaa gcggttagta 361 tgcagccgtc acttagaagt gagtatgagt accctgtttt ttctcatgtt caggcaggga 421 tgttctcacc tgagcttaga acctttacca aaggtgatgc ggagagatgg gtaagcacaa 481 ccaaaaaagc cagtgattct gcattctggc ttgaggttga aggtaattcc atgaccgcac 541 caacaggctc caagccaagc tttcctgacg gaatgttaat tctcgttgac cctgagcagg 601 ctgttgagcc aggtgatttc tgcatagcca gacttggggg tgatgagttt accttcaaga 661 aactgatcag ggatagcggt caggtgtttt tacaaccact aaacccacag tacccaatga 721 tcccatgcaa tgagagttgt tccgttgtgg ggaaagttat cgctagtcag tggcctgaag 781 agacgtttgg cgctgcaaac gacgaaaact acgctttagt agcttaataa cgctgatagt 841 gctagtgtag atcgctacta gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga 901 ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctctactaga gtcacactgg 961 ctcaccttcg ggtgggcctt tctgcgttta ta //