LOCUS       BBa_K1022119            2299 bp    DNA     linear       22-Dec-2024
DEFINITION  pBAD: RBS : agrC : RBS : agrA : TT : pP2
ACCESSION   BBa_K1022119
VERSION     BBa_K1022119.1  
FEATURES             Location/Qualifiers
     promoter        1..131
                     /label=promoter
     misc_feature    40..57
                     /label=AraI1
     misc_feature    61..78
                     /label=AraI2
     promoter        1..130
                     /label=BBa_K206000
     RBS             139..150
                     /label=B0034-sequence RBS
     RBS             1411..1422
                     /label=B0034-sequence RBS
     misc_feature    1405..1410
                     /label=BamHI site
     CDS             157..1404
                     /label=agrC coding sequence
     CDS             1429..2058
                     /label=agrA coding sequence
     misc_feature    139..2058
                     /label=BBa_I746100
     stem_loop       2078..2121
                     /label=stem_loop
     misc_feature    2067..2146
                     /label=BBa_B0010
     terminator      2067..2146
                     /label=BBa_B0010
     stem_loop       2162..2181
                     /label=T7 TE
     misc_feature    2188..2188
                     /label=stop
     polyA_site      2182..2195
                     /label=polya
     misc_feature    2155..2195
                     /label=BBa_B0012
     terminator      2155..2195
                     /label=BBa_B0012
     promoter        2204..2299
                     /label=BBa_I746104
     misc_feature    139..2299
                     /label=BBa_I746103
ORIGIN
        1 acattgatta tttgcacggc gtcacacttt gctatgccat agcaagatag tccataagat
       61 tagcggatcc tacctgacgc tttttatcgc aactctctac tgtttctcca taccgttttt
      121 ttgggctagc tactagagaa agaggagaaa tactagatga ttctgatgtt caccattcca
      181 gcaattatta gcggtattaa gtacagcaag ctggattatt tcttcatcat cgtaatctct
      241 accctgtccc tgttcctgtt caaaatgttc gattccgcga gcctgattat cctgacttcc
      301 ttcatcatca tcatgtattt cgtaaaaatc aaatggtata gcatcctgct gatcatgacc
      361 tcccagatta tcctgtactg tgccaactac atgtatatcg ttatctacgc gtacatcacg
      421 aaaatctccg attctatctt cgttatcttt ccgtctttct tcgtggtgta cgttacgatc
      481 tctattctgt tttcctatat tatcaatcgc gttctgaaaa agatctctac tccgtacctg
      541 attctgaaca aaggttttct gatcgtcatc tccactatcc tgctgctgac cttctctctg
      601 ttcttctttt actctcagat caactccgat gaggcaaaag tcatccgtca gtacagcttc
      661 atctttatcg gtattactat cttcctgagc attctgactt tcgttatcag ccagttcctg
      721 ctgaaagaaa tgaagtacaa gcgcaaccaa gaagaaatcg agacttacta cgagtacacc
      781 ctgaagattg aagcgatcaa caacgaaatg cgtaaatttc gtcacgacta cgttaacatt
      841 ctgaccaccc tgagcgaata catccgtgaa gacgacatgc ctggcctgcg tgattatttc
      901 aacaaaaaca ttgtgccgat gaaagataac ctgcaaatga acgctattaa gctgaacggt
      961 atcgaaaacc tgaaagtacg tgagatcaag ggtctgatta ccgctaaaat cctgcgtgcg
     1021 caggaaatga acatcccaat tagcatcgaa atcccagacg aagtttctag cattaacctg
     1081 aacatgattg acctgtctcg tagcatcggc atcatcctgg acaacgctat cgaagcttcc
     1141 actgaaatcg acgacccgat tattcgcgtt gcttttatcg agagcgaaaa ctccgtgacc
     1201 tttatcgtga tgaacaaatg cgcggacgat atcccgcgta ttcacgaact gttccaggaa
     1261 tctttcagca ccaagggtga aggtcgcggc ctgggcctgt ccaccctgaa agagatcgcg
     1321 gataacgccg acaacgttct gctggacact attatcgaaa acggtttctt cattcagaaa
     1381 gtcgaaatca tcaataacta ataaggatcc aaagaggaga aatactagat ggaaatcgca
     1441 ctggctacgg acaatccata tgaggttctg gaacaggcta aaaacatgaa cgacatcggc
     1501 tgctatttcc tggatattca gctgagcacc gacatcaacg gtatcaaact gggctctgaa
     1561 atccgtaaac atgatccggt tggtaacatt attttcgtga cttctcattc tgagctgacc
     1621 tatctgacgt tcgtatacaa ggttgcagcc atggatttca tctttaaaga cgatccggca
     1681 gagctgcgta cgcgcatcat cgactgtctg gaaactgcgc acacgcgcct gcaactgctg
     1741 tccaaagata actccgtgga aaccattgaa ctgaaacgtg gcagcaacag cgtttacgtt
     1801 cagtacgatg atatcatgtt cttcgaaagc tccactaagt cccaccgtct gatcgcgcat
     1861 ctggataatc gtcagatcga gttctatggc aacctgaaag agctgagcca gctggacgat
     1921 cgcttcttcc gttgtcataa ctccttcgtt gttaaccgtc acaacatcga atccatcgac
     1981 tccaaagagc gtattgtata cttcaagaac aaagaacact gttatgcgtc tgtacgcaat
     2041 gtcaagaaaa tctaataata ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa
     2101 agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctctact agagtcacac
     2161 tggctcacct tcgggtgggc ctttctgcgt ttatatacta gagattaaat acaaattaca
     2221 tttaacagtt aagtatttat ttcctacagt taggcaatat aatgataaaa gattgtacta
     2281 aatcgtataa tgacagtga
//