LOCUS BBa_K1022121 3523 bp DNA linear 05-Nov-2024 DEFINITION pBAD: Agr A : Agr C : pP2 : RBS: His6- SUMO: Signiferin: RBS TetR :TT ACCESSION BBa_K1022121 VERSION BBa_K1022121.1 FEATURES Location/Qualifiers promoter 1..131 /label=promoter misc_feature 1..130 /label=BBa_K206000 misc_feature 40..57 /label=AraI1 misc_feature 61..78 /label=AraI2 misc_feature 139..2299 /label=BBa_I746103 misc_feature 139..2058 /label=BBa_I746100 RBS 139..150 /label=B0034-sequence RBS CDS 157..1404 /label=agrC coding sequence misc_feature 1405..1410 /label=BamHI site RBS 1411..1422 /label=B0034-sequence RBS CDS 1429..2058 /label=agrA coding sequence misc_feature 2067..2146 /label=BBa_B0010 stem_loop 2078..2121 /label=stem_loop misc_feature 2155..2195 /label=BBa_B0012 stem_loop 2162..2181 /label=T7 TE polyA_site 2182..2195 /label=polya misc_feature 2188..2188 /label=stop misc_feature 2204..2299 /label=BBa_I746104 misc_feature 1..2299 /label=BBa_K1022119 RBS 2300..2313 /label=34 misc_feature 2314..2634 /label=His 6 SUMO misc_feature 2635..2683 /label=Signiferin RBS 2688..2691 /label=34 CDS 2705..3320 /label=Tet R misc_feature 3395..3474 /label=B0010 misc_feature 3483..3523 /label=B0012 ORIGIN 1 acattgatta tttgcacggc gtcacacttt gctatgccat agcaagatag tccataagat 61 tagcggatcc tacctgacgc tttttatcgc aactctctac tgtttctcca taccgttttt 121 ttgggctagc tactagagaa agaggagaaa tactagatga ttctgatgtt caccattcca 181 gcaattatta gcggtattaa gtacagcaag ctggattatt tcttcatcat cgtaatctct 241 accctgtccc tgttcctgtt caaaatgttc gattccgcga gcctgattat cctgacttcc 301 ttcatcatca tcatgtattt cgtaaaaatc aaatggtata gcatcctgct gatcatgacc 361 tcccagatta tcctgtactg tgccaactac atgtatatcg ttatctacgc gtacatcacg 421 aaaatctccg attctatctt cgttatcttt ccgtctttct tcgtggtgta cgttacgatc 481 tctattctgt tttcctatat tatcaatcgc gttctgaaaa agatctctac tccgtacctg 541 attctgaaca aaggttttct gatcgtcatc tccactatcc tgctgctgac cttctctctg 601 ttcttctttt actctcagat caactccgat gaggcaaaag tcatccgtca gtacagcttc 661 atctttatcg gtattactat cttcctgagc attctgactt tcgttatcag ccagttcctg 721 ctgaaagaaa tgaagtacaa gcgcaaccaa gaagaaatcg agacttacta cgagtacacc 781 ctgaagattg aagcgatcaa caacgaaatg cgtaaatttc gtcacgacta cgttaacatt 841 ctgaccaccc tgagcgaata catccgtgaa gacgacatgc ctggcctgcg tgattatttc 901 aacaaaaaca ttgtgccgat gaaagataac ctgcaaatga acgctattaa gctgaacggt 961 atcgaaaacc tgaaagtacg tgagatcaag ggtctgatta ccgctaaaat cctgcgtgcg 1021 caggaaatga acatcccaat tagcatcgaa atcccagacg aagtttctag cattaacctg 1081 aacatgattg acctgtctcg tagcatcggc atcatcctgg acaacgctat cgaagcttcc 1141 actgaaatcg acgacccgat tattcgcgtt gcttttatcg agagcgaaaa ctccgtgacc 1201 tttatcgtga tgaacaaatg cgcggacgat atcccgcgta ttcacgaact gttccaggaa 1261 tctttcagca ccaagggtga aggtcgcggc ctgggcctgt ccaccctgaa agagatcgcg 1321 gataacgccg acaacgttct gctggacact attatcgaaa acggtttctt cattcagaaa 1381 gtcgaaatca tcaataacta ataaggatcc aaagaggaga aatactagat ggaaatcgca 1441 ctggctacgg acaatccata tgaggttctg gaacaggcta aaaacatgaa cgacatcggc 1501 tgctatttcc tggatattca gctgagcacc gacatcaacg gtatcaaact gggctctgaa 1561 atccgtaaac atgatccggt tggtaacatt attttcgtga cttctcattc tgagctgacc 1621 tatctgacgt tcgtatacaa ggttgcagcc atggatttca tctttaaaga cgatccggca 1681 gagctgcgta cgcgcatcat cgactgtctg gaaactgcgc acacgcgcct gcaactgctg 1741 tccaaagata actccgtgga aaccattgaa ctgaaacgtg gcagcaacag cgtttacgtt 1801 cagtacgatg atatcatgtt cttcgaaagc tccactaagt cccaccgtct gatcgcgcat 1861 ctggataatc gtcagatcga gttctatggc aacctgaaag agctgagcca gctggacgat 1921 cgcttcttcc gttgtcataa ctccttcgtt gttaaccgtc acaacatcga atccatcgac 1981 tccaaagagc gtattgtata cttcaagaac aaagaacact gttatgcgtc tgtacgcaat 2041 gtcaagaaaa tctaataata ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa 2101 agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctctact agagtcacac 2161 tggctcacct tcgggtgggc ctttctgcgt ttatatacta gagattaaat acaaattaca 2221 tttaacagtt aagtatttat ttcctacagt taggcaatat aatgataaaa gattgtacta 2281 aatcgtataa tgacagtgaa ggagaaatac tagatggcta gcatgcatca tcatcatcat 2341 cattcggact cagaagtcaa tcaagaagct aagccagagg tcaagccaga agtcaagcct 2401 gagactcaca tcaatttaaa ggtgtccgat ggatcttcag agatcttctt caagatcaaa 2461 aagaccactc ctttaagaag gctgatggaa gcgttcgcta aacgtcaggg taaggaaatg 2521 gactccttaa gattcttgta cgacggtatt aggattcaag ctgatcagac ccctgaagat 2581 ttggacatgg atgataacga tattattgag gctcacagag aacagaccgg tggtatcatc 2641 ggtcacctga tcaaaaccgc gctgggtatg ctgggtctgt aataaagagg agaaatacta 2701 gatgtccaga ttagataaaa gtaaagtgat taacagcgca ttagagctgc ttaatgaggt 2761 cggaatcgaa ggtttaacaa cccgtaaact cgcccagaag ctaggtgtag agcagcctac 2821 attgtattgg catgtaaaaa ataagcgggc tttgctcgac gccttagcca ttgagatgtt 2881 agataggcac catactcact tttgcccttt agaaggggaa agctggcaag attttttacg 2941 taataacgct aaaagtttta gatgtgcttt actaagtcat cgcgatggag caaaagtaca 3001 tttaggtaca cggcctacag aaaaacagta tgaaactctc gaaaatcaat tagccttttt 3061 atgccaacaa ggtttttcac tagagaatgc attatatgca ctcagcgctg tggggcattt 3121 tactttaggt tgcgtattgg aagatcaaga gcatcaagtc gctaaagaag aaagggaaac 3181 acctactact gatagtatgc cgccattatt acgacaagct atcgaattat ttgatcacca 3241 aggtgcagag ccagccttct tattcggcct tgaattgatc atatgcggat tagaaaaaca 3301 acttaaatgt gaaagtgggt ccgctgcaaa cgacgaaaac tacgctttag tagcttaata 3361 acactgatag tgctagtgta gatcactact agagccaggc atcaaataaa acgaaaggct 3421 cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctctactag 3481 agtcacactg gctcaccttc gggtgggcct ttctgcgttt ata //