##gff-version 3
##sequence-region BBa_K1022121 1 3523
BBa_K1022121	.	stem_loop	2162	2181	.	+	0	ID=annotation2356452;Name=T7 TE
BBa_K1022121	.	sequence_feature	2635	2683	.	+	0	ID=annotation2356483;Name=Signiferin 
BBa_K1022121	.	stop_codon	3395	3474	.	+	0	ID=annotation2356506;Name=B0010
BBa_K1022121	.	operator	61	78	.	+	0	ID=annotation2356441;Name=AraI2
BBa_K1022121	.	stop_codon	3483	3523	.	+	0	ID=annotation2356521;Name=B0012
BBa_K1022121	.	promoter	1	131	.	+	0	ID=annotation2356438;Name=promoter
BBa_K1022121	.	operator	40	57	.	+	0	ID=annotation2356440;Name=AraI1
BBa_K1022121	.	ribosome_entry_site	2688	2691	.	+	0	ID=annotation2356492;Name=34
BBa_K1022121	.	engineered_region	139	2058	.	+	0	ID=annotation2356443;Name=BBa_I746100
BBa_K1022121	.	engineered_region	2155	2195	.	+	0	ID=annotation2356451;Name=BBa_B0012
BBa_K1022121	.	sequence_feature	1405	1410	.	+	0	ID=annotation2356446;Name=BamHI site
BBa_K1022121	.	CDS	1429	2058	.	+	0	ID=annotation2356448;Name=agrA coding sequence
BBa_K1022121	.	CDS	2705	3320	.	+	0	ID=annotation2356493;Name=Tet R
BBa_K1022121	.	CDS	157	1404	.	+	0	ID=annotation2356445;Name=agrC coding sequence
BBa_K1022121	.	ribosome_entry_site	1411	1422	.	+	0	ID=annotation2356447;Name=B0034-sequence RBS
BBa_K1022121	.	ribosome_entry_site	2300	2313	.	+	0	ID=annotation2356457;Name=34
BBa_K1022121	.	engineered_region	1	130	.	+	0	ID=annotation2356439;Name=BBa_K206000
BBa_K1022121	.	engineered_region	139	2299	.	+	0	ID=annotation2356442;Name=BBa_I746103
BBa_K1022121	.	engineered_region	2204	2299	.	+	0	ID=annotation2356455;Name=BBa_I746104
BBa_K1022121	.	polyA_site	2182	2195	.	+	0	ID=annotation2356453;Name=polya
BBa_K1022121	.	stem_loop	2078	2121	.	+	0	ID=annotation2356450;Name=stem_loop
BBa_K1022121	.	tag	2314	2634	.	+	0	ID=annotation2356458;Name=His 6 SUMO 
BBa_K1022121	.	engineered_region	2067	2146	.	+	0	ID=annotation2356449;Name=BBa_B0010
BBa_K1022121	.	ribosome_entry_site	139	150	.	+	0	ID=annotation2356444;Name=B0034-sequence RBS
BBa_K1022121	.	stop_codon	2188	2188	.	+	0	ID=annotation2356454;Name=stop
BBa_K1022121	.	engineered_region	1	2299	.	+	0	ID=annotation2356456;Name=BBa_K1022119
>BBa_K1022121
acattgattatttgcacggcgtcacactttgctatgccatagcaagatagtccataagattagcggatcctacctgacg
ctttttatcgcaactctctactgtttctccataccgtttttttgggctagctactagagaaagaggagaaatactagat
gattctgatgttcaccattccagcaattattagcggtattaagtacagcaagctggattatttcttcatcatcgtaatc
tctaccctgtccctgttcctgttcaaaatgttcgattccgcgagcctgattatcctgacttccttcatcatcatcatgt
atttcgtaaaaatcaaatggtatagcatcctgctgatcatgacctcccagattatcctgtactgtgccaactacatgta
tatcgttatctacgcgtacatcacgaaaatctccgattctatcttcgttatctttccgtctttcttcgtggtgtacgtt
acgatctctattctgttttcctatattatcaatcgcgttctgaaaaagatctctactccgtacctgattctgaacaaag
gttttctgatcgtcatctccactatcctgctgctgaccttctctctgttcttcttttactctcagatcaactccgatga
ggcaaaagtcatccgtcagtacagcttcatctttatcggtattactatcttcctgagcattctgactttcgttatcagc
cagttcctgctgaaagaaatgaagtacaagcgcaaccaagaagaaatcgagacttactacgagtacaccctgaagattg
aagcgatcaacaacgaaatgcgtaaatttcgtcacgactacgttaacattctgaccaccctgagcgaatacatccgtga
agacgacatgcctggcctgcgtgattatttcaacaaaaacattgtgccgatgaaagataacctgcaaatgaacgctatt
aagctgaacggtatcgaaaacctgaaagtacgtgagatcaagggtctgattaccgctaaaatcctgcgtgcgcaggaaa
tgaacatcccaattagcatcgaaatcccagacgaagtttctagcattaacctgaacatgattgacctgtctcgtagcat
cggcatcatcctggacaacgctatcgaagcttccactgaaatcgacgacccgattattcgcgttgcttttatcgagagc
gaaaactccgtgacctttatcgtgatgaacaaatgcgcggacgatatcccgcgtattcacgaactgttccaggaatctt
tcagcaccaagggtgaaggtcgcggcctgggcctgtccaccctgaaagagatcgcggataacgccgacaacgttctgct
ggacactattatcgaaaacggtttcttcattcagaaagtcgaaatcatcaataactaataaggatccaaagaggagaaa
tactagatggaaatcgcactggctacggacaatccatatgaggttctggaacaggctaaaaacatgaacgacatcggct
gctatttcctggatattcagctgagcaccgacatcaacggtatcaaactgggctctgaaatccgtaaacatgatccggt
tggtaacattattttcgtgacttctcattctgagctgacctatctgacgttcgtatacaaggttgcagccatggatttc
atctttaaagacgatccggcagagctgcgtacgcgcatcatcgactgtctggaaactgcgcacacgcgcctgcaactgc
tgtccaaagataactccgtggaaaccattgaactgaaacgtggcagcaacagcgtttacgttcagtacgatgatatcat
gttcttcgaaagctccactaagtcccaccgtctgatcgcgcatctggataatcgtcagatcgagttctatggcaacctg
aaagagctgagccagctggacgatcgcttcttccgttgtcataactccttcgttgttaaccgtcacaacatcgaatcca
tcgactccaaagagcgtattgtatacttcaagaacaaagaacactgttatgcgtctgtacgcaatgtcaagaaaatcta
ataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtc
ggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagattaaatac
aaattacatttaacagttaagtatttatttcctacagttaggcaatataatgataaaagattgtactaaatcgtataat
gacagtgaaggagaaatactagatggctagcatgcatcatcatcatcatcattcggactcagaagtcaatcaagaagct
aagccagaggtcaagccagaagtcaagcctgagactcacatcaatttaaaggtgtccgatggatcttcagagatcttct
tcaagatcaaaaagaccactcctttaagaaggctgatggaagcgttcgctaaacgtcagggtaaggaaatggactcctt
aagattcttgtacgacggtattaggattcaagctgatcagacccctgaagatttggacatggatgataacgatattatt
gaggctcacagagaacagaccggtggtatcatcggtcacctgatcaaaaccgcgctgggtatgctgggtctgtaataaa
gaggagaaatactagatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaa
tcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataa
gcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagc
tggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatt
taggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcact
agagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtc
gctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcacc
aaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgg
gtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagcca
ggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctcta
ctagagtcacactggctcaccttcgggtgggcctttctgcgtttata