BBa_K1022122
1
BBa_K1022122
pBAD: Agr A : Agr C : pP2 : RBS: His6- SUMO: Magainin II : RBS TetR :TT
2013-09-19T11:00:00Z
2015-05-08T01:08:45Z
Clonned
This biobrick is one of the final constructs in Project Peptidor by Team TU Delft iGEM 2013 to check the sensing of AIP by E.coli and then production of the peptides followed by TetR production to activated the next set of the constructs.
false
false
_1329_
0
15691
9
It's complicated
false
The Biobrick Bba_K1022106 is PCRed using designed primer to remove the pT7 promoter and then Ligated with the Biobrick Bba_K1022119
false
Maithili Krishnan
annotation2356570
1
BBa_K206000
range2356570
1
1
130
annotation2356580
1
BBa_B0010
range2356580
1
2067
2146
annotation2356583
1
T7 TE
range2356583
1
2162
2181
annotation2356575
1
B0034-sequence RBS
range2356575
1
139
150
annotation2356702
1
B0012
range2356702
1
3507
3547
annotation2356590
1
Magainin II
range2356590
1
2638
2707
annotation2356652
1
Tet R
range2356652
1
2729
3344
annotation2356589
1
His 6 - SUMO
range2356589
1
2314
2634
annotation2356651
1
34
range2356651
1
2712
2715
annotation2356569
1
promoter
range2356569
1
1
131
annotation2356585
1
stop
range2356585
1
2188
2188
annotation2356579
1
agrA coding sequence
range2356579
1
1429
2058
annotation2356581
1
stem_loop
range2356581
1
2078
2121
annotation2356577
1
BamHI site
range2356577
1
1405
1410
annotation2356588
1
pT7
range2356588
1
2300
2313
annotation2356587
1
BBa_K1022119
range2356587
1
1
2299
annotation2356586
1
BBa_I746104
range2356586
1
2204
2299
annotation2356584
1
polya
range2356584
1
2182
2195
annotation2356684
1
B0010
range2356684
1
3419
3498
annotation2356571
1
AraI1
range2356571
1
40
57
annotation2356574
1
BBa_I746100
range2356574
1
139
2058
annotation2356582
1
BBa_B0012
range2356582
1
2155
2195
annotation2356576
1
agrC coding sequence
range2356576
1
157
1404
annotation2356578
1
B0034-sequence RBS
range2356578
1
1411
1422
annotation2356572
1
AraI2
range2356572
1
61
78
annotation2356573
1
BBa_I746103
range2356573
1
139
2299
BBa_K1022122_sequence
1
acattgattatttgcacggcgtcacactttgctatgccatagcaagatagtccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccataccgtttttttgggctagctactagagaaagaggagaaatactagatgattctgatgttcaccattccagcaattattagcggtattaagtacagcaagctggattatttcttcatcatcgtaatctctaccctgtccctgttcctgttcaaaatgttcgattccgcgagcctgattatcctgacttccttcatcatcatcatgtatttcgtaaaaatcaaatggtatagcatcctgctgatcatgacctcccagattatcctgtactgtgccaactacatgtatatcgttatctacgcgtacatcacgaaaatctccgattctatcttcgttatctttccgtctttcttcgtggtgtacgttacgatctctattctgttttcctatattatcaatcgcgttctgaaaaagatctctactccgtacctgattctgaacaaaggttttctgatcgtcatctccactatcctgctgctgaccttctctctgttcttcttttactctcagatcaactccgatgaggcaaaagtcatccgtcagtacagcttcatctttatcggtattactatcttcctgagcattctgactttcgttatcagccagttcctgctgaaagaaatgaagtacaagcgcaaccaagaagaaatcgagacttactacgagtacaccctgaagattgaagcgatcaacaacgaaatgcgtaaatttcgtcacgactacgttaacattctgaccaccctgagcgaatacatccgtgaagacgacatgcctggcctgcgtgattatttcaacaaaaacattgtgccgatgaaagataacctgcaaatgaacgctattaagctgaacggtatcgaaaacctgaaagtacgtgagatcaagggtctgattaccgctaaaatcctgcgtgcgcaggaaatgaacatcccaattagcatcgaaatcccagacgaagtttctagcattaacctgaacatgattgacctgtctcgtagcatcggcatcatcctggacaacgctatcgaagcttccactgaaatcgacgacccgattattcgcgttgcttttatcgagagcgaaaactccgtgacctttatcgtgatgaacaaatgcgcggacgatatcccgcgtattcacgaactgttccaggaatctttcagcaccaagggtgaaggtcgcggcctgggcctgtccaccctgaaagagatcgcggataacgccgacaacgttctgctggacactattatcgaaaacggtttcttcattcagaaagtcgaaatcatcaataactaataaggatccaaagaggagaaatactagatggaaatcgcactggctacggacaatccatatgaggttctggaacaggctaaaaacatgaacgacatcggctgctatttcctggatattcagctgagcaccgacatcaacggtatcaaactgggctctgaaatccgtaaacatgatccggttggtaacattattttcgtgacttctcattctgagctgacctatctgacgttcgtatacaaggttgcagccatggatttcatctttaaagacgatccggcagagctgcgtacgcgcatcatcgactgtctggaaactgcgcacacgcgcctgcaactgctgtccaaagataactccgtggaaaccattgaactgaaacgtggcagcaacagcgtttacgttcagtacgatgatatcatgttcttcgaaagctccactaagtcccaccgtctgatcgcgcatctggataatcgtcagatcgagttctatggcaacctgaaagagctgagccagctggacgatcgcttcttccgttgtcataactccttcgttgttaaccgtcacaacatcgaatccatcgactccaaagagcgtattgtatacttcaagaacaaagaacactgttatgcgtctgtacgcaatgtcaagaaaatctaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagattaaatacaaattacatttaacagttaagtatttatttcctacagttaggcaatataatgataaaagattgtactaaatcgtataatgacagtgaaggagaaatactagatggctagcatgcatcatcatcatcatcattcggactcagaagtcaatcaagaagctaagccagaggtcaagccagaagtcaagcctgagactcacatcaatttaaaggtgtccgatggatcttcagagatcttcttcaagatcaaaaagaccactcctttaagaaggctgatggaagcgttcgctaaacgtcagggtaaggaaatggactccttaagattcttgtacgacggtattaggattcaagctgatcagacccctgaagatttggacatggatgataacgatattattgaggctcacagagaacagaccggtggtggtatcggtaaattcctgcactctgcgaaaaaattcggtaaagcgttcgttggtcagatcatgaactcttaataaagaggagaaatactagatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z