LOCUS       BBa_K1022123            3608 bp    DNA     linear       06-Nov-2024
DEFINITION  pTet: cI: TT : pcI: Ulp : Lysis
ACCESSION   BBa_K1022123
VERSION     BBa_K1022123.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    67..70
                     /label=conserved
     RBS             63..74
                     /label=BBa_B0034
     promoter        1..74
                     /label=BBa_J13002
     CDS             84..791
                     /label=cI lambda
     misc_feature    792..824
                     /label=LVA
     misc_feature    831..855
                     /label=Help:Barcodes
     CDS             81..855
                     /label=BBa_C0051
     stem_loop       875..918
                     /label=stem_loop
     misc_feature    864..943
                     /label=BBa_B0010
     terminator      864..943
                     /label=BBa_B0010
     stem_loop       959..978
                     /label=T7 TE
     misc_feature    985..985
                     /label=stop
     polyA_site      979..992
                     /label=polya
     misc_feature    952..992
                     /label=BBa_B0012
     terminator      952..992
                     /label=BBa_B0012
     terminator      864..992
                     /label=BBa_B0015
     misc_feature    1..992
                     /label=BBa_K1022108
     promoter        1001..1049
                     /label=cI
     RBS             1058..1074
                     /label=34
     misc_feature    1075..1815
                     /label=Ulp
     misc_feature    1001..1815
                     /label=BBa_K1022107
     misc_feature    1..1815
                     /label=BBa_K1022115
     misc_feature    1824..3608
                     /label=BBa_K1022109
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agaaagagga gaaatactag atgagcacaa aaaagaaacc attaacacaa gagcagcttg
      121 aggacgcacg tcgccttaaa gcaatttatg aaaaaaagaa aaatgaactt ggcttatccc
      181 aggaatctgt cgcagacaag atggggatgg ggcagtcagg cgttggtgct ttatttaatg
      241 gcatcaatgc attaaatgct tataacgccg cattgcttgc aaaaattctc aaagttagcg
      301 ttgaagaatt tagcccttca atcgccagag aaatctacga gatgtatgaa gcggttagta
      361 tgcagccgtc acttagaagt gagtatgagt accctgtttt ttctcatgtt caggcaggga
      421 tgttctcacc tgagcttaga acctttacca aaggtgatgc ggagagatgg gtaagcacaa
      481 ccaaaaaagc cagtgattct gcattctggc ttgaggttga aggtaattcc atgaccgcac
      541 caacaggctc caagccaagc tttcctgacg gaatgttaat tctcgttgac cctgagcagg
      601 ctgttgagcc aggtgatttc tgcatagcca gacttggggg tgatgagttt accttcaaga
      661 aactgatcag ggatagcggt caggtgtttt tacaaccact aaacccacag tacccaatga
      721 tcccatgcaa tgagagttgt tccgttgtgg ggaaagttat cgctagtcag tggcctgaag
      781 agacgtttgg cgctgcaaac gacgaaaact acgctttagt agcttaataa cgctgatagt
      841 gctagtgtag atcgctacta gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga
      901 ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctctactaga gtcacactgg
      961 ctcaccttcg ggtgggcctt tctgcgttta tatactagag taacaccgtg cgtgttgact
     1021 attttacctc tggcggtgat aatggttgct taccggaaag aggagaaata ctagatggct
     1081 agccttgttc ctgaattaaa tgaaaaagac gatgaccaag tacaaaaagc tttggcatcg
     1141 agagaaaata ctcagttaat gaatagagat aatatagaga taacagtacg tgattttaag
     1201 accttggcac cacgaagatg gctaaatgac actatcattg agttttttat gaaatacatt
     1261 gaaaaatcta cccctaatac agtggcgttt aattcatttt tctataccaa tttatcagaa
     1321 aggggttatc aaggcgtccg gaggtggatg aagagaaaga agacacaaat tgataaactt
     1381 gataaaatct ttacaccaat aaatttgaac caatcccact gggcgttggg cataattgat
     1441 ttaaaaaaga aaactatagg ttacgtagat tcattatcga atggtccaaa tgctatgagt
     1501 ttcgctatac tgactgactt gcaaaaatat gttatggagg aaagtaagca tacaatagga
     1561 gaagactttg atttgattca tttagattgt ccgcagcaac caaatggcta cgactgtgga
     1621 atatatgttt gtatgaatac tctctatgga agtgcagatg cgccattgga ttttgattat
     1681 aaagatgcga ttaggatgag aagatttatt gcccatttga ttttaaccga cgctttaaaa
     1741 cgggatccga actcgagctc cgtcgacaag cttgcggccg cactcgagca ccaccaccac
     1801 caccactgag tacattacta gagcttaaaa ggagggtcta tggcagcacc tagaatatca
     1861 ttttcgccct ctgatattct atttggtgtt ctcgatcgct tgttcaaaga taacgctacc
     1921 gggaaggttc ttgcttcccg ggtagctgtc gtaattcttt tgtttataat ggcgattgtt
     1981 tggtataggg gagatagttt ctttgagtac tataagcaat caaagtatga aacatacagt
     2041 gaaattattg aaaaggaaag aactgcacgc tttgaatctg tcgccctgga acaactccag
     2101 atagttcata tatcatctga ggcagacttt agtgcggtgt attctttccg ccctaaaaac
     2161 ttaaactatt ttgttgatat tatagcatac gaaggaaaat taccttcaac aataagtgaa
     2221 aaatcacttg gaggatatcc tgttgataaa actatggatg aatatacagt tcatttaaat
     2281 ggacgtcatt attattccaa ctcaaaattt gcttttttac caactaaaaa gcctactccc
     2341 gaaataaact acatgtacag ttgtccatat tttaatttgg ataatatcta tgctggaacg
     2401 ataaccatgt actggtatag aaatgatcat ataagtaatg accgccttga atcaatatgt
     2461 gctcaggcgg ccagaatatt aggaagggct aaataattat actagagata cttaggaggt
     2521 attatgaata tatttgaaat gttacgtata gatgaaggtc ttagacttaa aatctataaa
     2581 gacacagaag gctattacac tattggcatc ggtcatttgc ttacaaaaag tccatcactt
     2641 aatgctgcta aatctgaatt agataaagct attgggcgta attgcaatgg tgtaattaca
     2701 aaagatgagg ctgaaaaact ctttaatcag gatgttgatg ctgctgttcg cggaatcctg
     2761 agaaatgcta aattaaaacc ggtttatgat tctcttgatg cggttcgtcg ctgtgcattg
     2821 attaatatgg ttttccaaat gggagaaacc ggtgtggcag gatttactaa ctctttacgt
     2881 atgcttcaac aaaaacgctg ggatgaagca gcagttaact tagctaaaag tagatggtat
     2941 aatcaaacac ctaatcgcgc aaaacgagtc attacaacgt ttagaactgg cacttgggac
     3001 gcgtataaaa atctataaag ctactagagc caggcatcaa ataaaacgaa aggctcagtc
     3061 gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca
     3121 cactggctca ccttcgggtg ggcctttctg cgtttatata ctagagttga cagctagctc
     3181 agtcctaggg actatgctag ctactagagg ataggaggcc tttatggcct taaaagcaac
     3241 agcacttttt gccatgctag gattgtcatt tgttttatct ccatcgattg aagcgaatgt
     3301 cgatcctcat tttgataaat ttatggaatc tggtattagg cacgtttata tgctttttga
     3361 aaataaaagc gtagaatcgt ctgaacaatt ctatagtttt atgagaacga cctataaaaa
     3421 tgacccgtgc tcttctgatt ttgaatgtat agagcgaggc gcggagatgg cacaatcata
     3481 cgctagaatt atgaacatta aattggagac tgaatgaaat tactagagcc aggcatcaaa
     3541 taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga
     3601 acgctctc
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