LOCUS BBa_K1022124 4891 bp DNA linear 05-Nov-2024 DEFINITION pT7: RBS: His6- SUMO: MaximinH5: RBS TetR :TT : pTet: cI: TT : pcI: Ulp : Lysis ACCESSION BBa_K1022124 VERSION BBa_K1022124.1 FEATURES Location/Qualifiers promoter 1..28 /label=T7 RBS 29..42 /label=34 misc_feature 43..363 /label=His6:SUMO misc_feature 364..427 /label=MaximinH5 misc_feature 1..427 /label=BBa_K1022101 misc_feature 440..443 /label=conserved RBS 436..447 /label=BBa_B0034 CDS 457..1073 /label=tetR misc_feature 1074..1107 /label=SsrA misc_feature 1114..1138 /label=Help:Barcodes CDS 454..1113 /label=BBa_C0040 stem_loop 1158..1201 /label=stem_loop misc_feature 1147..1226 /label=BBa_B0010 terminator 1147..1226 /label=BBa_B0010 stem_loop 1242..1261 /label=T7 TE misc_feature 1268..1268 /label=stop polyA_site 1262..1275 /label=polya misc_feature 1235..1275 /label=BBa_B0012 terminator 1235..1275 /label=BBa_B0012 misc_feature 436..1275 /label=BBa_P0440 misc_feature 1..1275 /label=BBa_K1022104 misc_feature 1284..1302 /label=TetR 1 misc_feature 1284..1337 /label=BBa_R0040 promoter 1303..1308 /label=-35 misc_feature 1309..1327 /label=TetR 2 promoter 1326..1331 /label=-10 promoter 1284..1337 /label=BBa_R0040 misc_feature 1350..1353 /label=conserved RBS 1346..1357 /label=BBa_B0034 promoter 1284..1357 /label=BBa_J13002 CDS 1367..2074 /label=cI lambda misc_feature 2075..2107 /label=LVA misc_feature 2114..2138 /label=Help:Barcodes CDS 1364..2138 /label=BBa_C0051 stem_loop 2158..2201 /label=stem_loop misc_feature 2147..2226 /label=BBa_B0010 terminator 2147..2226 /label=BBa_B0010 stem_loop 2242..2261 /label=T7 TE misc_feature 2268..2268 /label=stop polyA_site 2262..2275 /label=polya misc_feature 2235..2275 /label=BBa_B0012 terminator 2235..2275 /label=BBa_B0012 terminator 2147..2275 /label=BBa_B0015 misc_feature 1284..2275 /label=BBa_K1022108 promoter 2284..2332 /label=cI RBS 2341..2357 /label=34 misc_feature 2358..3098 /label=Ulp misc_feature 2284..3098 /label=BBa_K1022107 misc_feature 1284..3098 /label=BBa_K1022115 misc_feature 3107..4891 /label=BBa_K1022109 misc_feature 1284..4891 /label=BBa_K1022123 ORIGIN 1 taatacgact cactataggg agagaaagag gagaaatact agatggctag catgcatcat 61 catcatcatc attcggactc agaagtcaat caagaagcta agccagaggt caagccagaa 121 gtcaagcctg agactcacat caatttaaag gtgtccgatg gatcttcaga gatcttcttc 181 aagatcaaaa agaccactcc tttaagaagg ctgatggaag cgttcgctaa acgtcagggt 241 aaggaaatgg actccttaag attcttgtac gacggtatta ggattcaagc tgatcagacc 301 cctgaagatt tggacatgga tgataacgat attattgagg ctcacagaga acagaccggt 361 ggtatcctgg gtccggttct gggtctggtt tctgacaccc tggacgacgt tctgggtatc 421 ctgtaattac tagagaaaga ggagaaatac tagatgtcca gattagataa aagtaaagtg 481 attaacagcg cattagagct gcttaatgag gtcggaatcg aaggtttaac aacccgtaaa 541 ctcgcccaga agctaggtgt agagcagcct acattgtatt ggcatgtaaa aaataagcgg 601 gctttgctcg acgccttagc cattgagatg ttagataggc accatactca cttttgccct 661 ttagaagggg aaagctggca agatttttta cgtaataacg ctaaaagttt tagatgtgct 721 ttactaagtc atcgcgatgg agcaaaagta catttaggta cacggcctac agaaaaacag 781 tatgaaactc tcgaaaatca attagccttt ttatgccaac aaggtttttc actagagaat 841 gcattatatg cactcagcgc tgtggggcat tttactttag gttgcgtatt ggaagatcaa 901 gagcatcaag tcgctaaaga agaaagggaa acacctacta ctgatagtat gccgccatta 961 ttacgacaag ctatcgaatt atttgatcac caaggtgcag agccagcctt cttattcggc 1021 cttgaattga tcatatgcgg attagaaaaa caacttaaat gtgaaagtgg gtccgctgca 1081 aacgacgaaa actacgcttt agtagcttaa taacactgat agtgctagtg tagatcacta 1141 ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta 1201 tctgttgttt gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc 1261 ctttctgcgt ttatatacta gagtccctat cagtgataga gattgacatc cctatcagtg 1321 atagagatac tgagcactac tagagaaaga ggagaaatac tagatgagca caaaaaagaa 1381 accattaaca caagagcagc ttgaggacgc acgtcgcctt aaagcaattt atgaaaaaaa 1441 gaaaaatgaa cttggcttat cccaggaatc tgtcgcagac aagatgggga tggggcagtc 1501 aggcgttggt gctttattta atggcatcaa tgcattaaat gcttataacg ccgcattgct 1561 tgcaaaaatt ctcaaagtta gcgttgaaga atttagccct tcaatcgcca gagaaatcta 1621 cgagatgtat gaagcggtta gtatgcagcc gtcacttaga agtgagtatg agtaccctgt 1681 tttttctcat gttcaggcag ggatgttctc acctgagctt agaaccttta ccaaaggtga 1741 tgcggagaga tgggtaagca caaccaaaaa agccagtgat tctgcattct ggcttgaggt 1801 tgaaggtaat tccatgaccg caccaacagg ctccaagcca agctttcctg acggaatgtt 1861 aattctcgtt gaccctgagc aggctgttga gccaggtgat ttctgcatag ccagacttgg 1921 gggtgatgag tttaccttca agaaactgat cagggatagc ggtcaggtgt ttttacaacc 1981 actaaaccca cagtacccaa tgatcccatg caatgagagt tgttccgttg tggggaaagt 2041 tatcgctagt cagtggcctg aagagacgtt tggcgctgca aacgacgaaa actacgcttt 2101 agtagcttaa taacgctgat agtgctagtg tagatcgcta ctagagccag gcatcaaata 2161 aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac 2221 gctctctact agagtcacac tggctcacct tcgggtgggc ctttctgcgt ttatatacta 2281 gagtaacacc gtgcgtgttg actattttac ctctggcggt gataatggtt gcttaccgga 2341 aagaggagaa atactagatg gctagccttg ttcctgaatt aaatgaaaaa gacgatgacc 2401 aagtacaaaa agctttggca tcgagagaaa atactcagtt aatgaataga gataatatag 2461 agataacagt acgtgatttt aagaccttgg caccacgaag atggctaaat gacactatca 2521 ttgagttttt tatgaaatac attgaaaaat ctacccctaa tacagtggcg tttaattcat 2581 ttttctatac caatttatca gaaaggggtt atcaaggcgt ccggaggtgg atgaagagaa 2641 agaagacaca aattgataaa cttgataaaa tctttacacc aataaatttg aaccaatccc 2701 actgggcgtt gggcataatt gatttaaaaa agaaaactat aggttacgta gattcattat 2761 cgaatggtcc aaatgctatg agtttcgcta tactgactga cttgcaaaaa tatgttatgg 2821 aggaaagtaa gcatacaata ggagaagact ttgatttgat tcatttagat tgtccgcagc 2881 aaccaaatgg ctacgactgt ggaatatatg tttgtatgaa tactctctat ggaagtgcag 2941 atgcgccatt ggattttgat tataaagatg cgattaggat gagaagattt attgcccatt 3001 tgattttaac cgacgcttta aaacgggatc cgaactcgag ctccgtcgac aagcttgcgg 3061 ccgcactcga gcaccaccac caccaccact gagtacatta ctagagctta aaaggagggt 3121 ctatggcagc acctagaata tcattttcgc cctctgatat tctatttggt gttctcgatc 3181 gcttgttcaa agataacgct accgggaagg ttcttgcttc ccgggtagct gtcgtaattc 3241 ttttgtttat aatggcgatt gtttggtata ggggagatag tttctttgag tactataagc 3301 aatcaaagta tgaaacatac agtgaaatta ttgaaaagga aagaactgca cgctttgaat 3361 ctgtcgccct ggaacaactc cagatagttc atatatcatc tgaggcagac tttagtgcgg 3421 tgtattcttt ccgccctaaa aacttaaact attttgttga tattatagca tacgaaggaa 3481 aattaccttc aacaataagt gaaaaatcac ttggaggata tcctgttgat aaaactatgg 3541 atgaatatac agttcattta aatggacgtc attattattc caactcaaaa tttgcttttt 3601 taccaactaa aaagcctact cccgaaataa actacatgta cagttgtcca tattttaatt 3661 tggataatat ctatgctgga acgataacca tgtactggta tagaaatgat catataagta 3721 atgaccgcct tgaatcaata tgtgctcagg cggccagaat attaggaagg gctaaataat 3781 tatactagag atacttagga ggtattatga atatatttga aatgttacgt atagatgaag 3841 gtcttagact taaaatctat aaagacacag aaggctatta cactattggc atcggtcatt 3901 tgcttacaaa aagtccatca cttaatgctg ctaaatctga attagataaa gctattgggc 3961 gtaattgcaa tggtgtaatt acaaaagatg aggctgaaaa actctttaat caggatgttg 4021 atgctgctgt tcgcggaatc ctgagaaatg ctaaattaaa accggtttat gattctcttg 4081 atgcggttcg tcgctgtgca ttgattaata tggttttcca aatgggagaa accggtgtgg 4141 caggatttac taactcttta cgtatgcttc aacaaaaacg ctgggatgaa gcagcagtta 4201 acttagctaa aagtagatgg tataatcaaa cacctaatcg cgcaaaacga gtcattacaa 4261 cgtttagaac tggcacttgg gacgcgtata aaaatctata aagctactag agccaggcat 4321 caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg 4381 gtgaacgctc tctactagag tcacactggc tcaccttcgg gtgggccttt ctgcgtttat 4441 atactagagt tgacagctag ctcagtccta gggactatgc tagctactag aggataggag 4501 gcctttatgg ccttaaaagc aacagcactt tttgccatgc taggattgtc atttgtttta 4561 tctccatcga ttgaagcgaa tgtcgatcct cattttgata aatttatgga atctggtatt 4621 aggcacgttt atatgctttt tgaaaataaa agcgtagaat cgtctgaaca attctatagt 4681 tttatgagaa cgacctataa aaatgacccg tgctcttctg attttgaatg tatagagcga 4741 ggcgcggaga tggcacaatc atacgctaga attatgaaca ttaaattgga gactgaatga 4801 aattactaga gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg 4861 ttttatctgt tgtttgtcgg tgaacgctct c //