BBa_K1022124
type: ComponentDefinition
persistent identity: BBa K1022124
display id: BBa_K1022124
version: 1
was generated by: iGEM to SBOL conversion
description: pT7: RBS: His6- SUMO: MaximinH5: RBS TetR :TT : pTet: cI: TT : pcI: Ulp : Lysis
created: 2013-09-19T11:00:00Z
modified: 2015-05-08T01:08:45Z
mutable provenance: Clonned
owned by: james
owned by: myers
mutable description: This biobrick is the one of the three final constructs of the project Peptidor by Team TU Delft iGEM 2013. This has a Promoter pT7 along with a His6 SUMO tagged peptide followed by a TetR which suppresses the timer module containing promoter pTET, cI is produced which suppresses pcI. Then Ulp protease is produced following by a Kill switch lysis device.
discontinued: false
dominant: false
group u list: _1329_
m user id: 0
owner id: 15691
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: -
star: false
creator: Maithili Krishnan
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_K1022123
component: BBa_K1022104
sequence annotation: BBa_K1022104
sequence annotation: BBa_K1022123
sequence: https://synbiohub.org/public/igem/BBa_K1022124_sequence/1

BBa_K1022123
type: Component
persistent identity: component2356774
display id: component2356774
version: 1
top level: BBa_K1022124
definition: BBa_K1022123
access: public

BBa_K1022104
type: Component
persistent identity: component2356747
display id: component2356747
version: 1
top level: BBa_K1022124
definition: BBa_K1022104
access: public

BBa_K1022104
type: SequenceAnnotation
persistent identity: annotation2356747
display id: annotation2356747
version: 1
top level: BBa_K1022124
location: https://synbiohub.org/public/igem/BBa_K1022124/annotation2356747/range2356747/1
component: BBa_K1022104

https://synbiohub.org/public/igem/BBa_K1022124/annotation2356747/range2356747/1
type: Range
persistent identity: range2356747
display id: range2356747
version: 1
top level: BBa_K1022124
direction: direction/reverse
start: 1
end: 1275
orientation: inline

BBa_K1022123
type: SequenceAnnotation
persistent identity: annotation2356774
display id: annotation2356774
version: 1
top level: BBa_K1022124
location: https://synbiohub.org/public/igem/BBa_K1022124/annotation2356774/range2356774/1
component: BBa_K1022123

https://synbiohub.org/public/igem/BBa_K1022124/annotation2356774/range2356774/1
type: Range
persistent identity: range2356774
display id: range2356774
version: 1
top level: BBa_K1022124
direction: direction/reverse
start: 1284
end: 4891
orientation: inline

BBa_K1022104
type: ComponentDefinition
persistent identity: BBa K1022104
display id: BBa_K1022104
version: 1
was generated by: iGEM to SBOL conversion
description: pT7: RBS: His6- SUMO: MaximinH5: RBS TetR :TT
created: 2013-09-04T11:00:00Z
modified: 2015-05-08T01:08:44Z
mutable provenance: Cloned from biobricks
owned by: james
owned by: myers
mutable description: This part codes for a primary construct to produce His 6-SUMO-Maximin H5 and TetR molecules followed by a double terminator, when triggered by IPTG.
discontinued: false
dominant: false
group u list: _1329_
m user id: 0
owner id: 15691
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: -
star: false
creator: Maithili Krishnan
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_P0440
component: BBa_K1022101
sequence annotation: BBa_K1022101
sequence annotation: BBa_P0440
sequence: https://synbiohub.org/public/igem/BBa_K1022104_sequence/1

BBa_P0440
type: Component
persistent identity: component2335019
display id: component2335019
version: 1
top level: BBa_K1022104
definition: BBa_P0440
access: public

BBa_K1022101
type: Component
persistent identity: component2335006
display id: component2335006
version: 1
top level: BBa_K1022104
definition: BBa_K1022101
access: public

BBa_K1022101
type: SequenceAnnotation
persistent identity: annotation2335006
display id: annotation2335006
version: 1
top level: BBa_K1022104
location: https://synbiohub.org/public/igem/BBa_K1022104/annotation2335006/range2335006/1
component: BBa_K1022101

https://synbiohub.org/public/igem/BBa_K1022104/annotation2335006/range2335006/1
type: Range
persistent identity: range2335006
display id: range2335006
version: 1
top level: BBa_K1022104
direction: direction/reverse
start: 1
end: 427
orientation: inline

BBa_P0440
type: SequenceAnnotation
persistent identity: annotation2335019
display id: annotation2335019
version: 1
top level: BBa_K1022104
location: https://synbiohub.org/public/igem/BBa_K1022104/annotation2335019/range2335019/1
component: BBa_P0440

https://synbiohub.org/public/igem/BBa_K1022104/annotation2335019/range2335019/1
type: Range
persistent identity: range2335019
display id: range2335019
version: 1
top level: BBa_K1022104
direction: direction/reverse
start: 436
end: 1275
orientation: inline

BBa_K1022123
type: ComponentDefinition
persistent identity: BBa K1022123
display id: BBa_K1022123
version: 1
was generated by: iGEM to SBOL conversion
description: pTet: cI: TT : pcI: Ulp : Lysis
created: 2013-09-19T11:00:00Z
modified: 2015-05-08T01:08:45Z
mutable provenance: Clonned
owned by: james
owned by: myers
mutable description: This bio-brick is a composite part for of the BBa_K1022115 and BBa_K1022109
discontinued: false
dominant: false
group u list: _1329_
m user id: 0
owner id: 15691
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: -
star: false
creator: Maithili Krishnan
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_K1022109
component: BBa_K1022115
sequence annotation: BBa_K1022115
sequence annotation: BBa_K1022109
sequence: https://synbiohub.org/public/igem/BBa_K1022123_sequence/1

BBa_K1022109
type: Component
persistent identity: component2356728
display id: component2356728
version: 1
top level: BBa_K1022123
definition: BBa_K1022109
access: public

BBa_K1022115
type: Component
persistent identity: component2356727
display id: component2356727
version: 1
top level: BBa_K1022123
definition: BBa_K1022115
access: public

BBa_K1022115
type: SequenceAnnotation
persistent identity: annotation2356727
display id: annotation2356727
version: 1
top level: BBa_K1022123
location: https://synbiohub.org/public/igem/BBa_K1022123/annotation2356727/range2356727/1
component: BBa_K1022115

https://synbiohub.org/public/igem/BBa_K1022123/annotation2356727/range2356727/1
type: Range
persistent identity: range2356727
display id: range2356727
version: 1
top level: BBa_K1022123
direction: direction/reverse
start: 1
end: 1815
orientation: inline

BBa_K1022109
type: SequenceAnnotation
persistent identity: annotation2356728
display id: annotation2356728
version: 1
top level: BBa_K1022123
location: https://synbiohub.org/public/igem/BBa_K1022123/annotation2356728/range2356728/1
component: BBa_K1022109

https://synbiohub.org/public/igem/BBa_K1022123/annotation2356728/range2356728/1
type: Range
persistent identity: range2356728
display id: range2356728
version: 1
top level: BBa_K1022123
direction: direction/reverse
start: 1824
end: 3608
orientation: inline

BBa_P0440
type: ComponentDefinition
persistent identity: BBa P0440
display id: BBa_P0440
version: 1
was generated by: iGEM to SBOL conversion
description: PoPS -> TetR [S0151]
created: 2004-04-26T11:00:00Z
modified: 2015-05-08T01:14:10Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: -- No description --
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_C0040
component: BBa_B0034
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_B0034
sequence annotation: BBa_C0040
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_P0440_sequence/1

BBa_C0040
type: Component
persistent identity: component944896
display id: component944896
version: 1
top level: BBa_P0440
definition: tetR
access: public

BBa_B0034
type: Component
persistent identity: component944886
display id: component944886
version: 1
top level: BBa_P0440
definition: BBa_B0034
access: public

BBa_B0012
type: Component
persistent identity: component944912
display id: component944912
version: 1
top level: BBa_P0440
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component944902
display id: component944902
version: 1
top level: BBa_P0440
definition: BBa_B0010
access: public

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation944886
display id: annotation944886
version: 1
top level: BBa_P0440
location: https://synbiohub.org/public/igem/BBa_P0440/annotation944886/range944886/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_P0440/annotation944886/range944886/1
type: Range
persistent identity: range944886
display id: range944886
version: 1
top level: BBa_P0440
direction: direction/forward
start: 1
end: 12
orientation: inline

BBa_C0040
type: SequenceAnnotation
persistent identity: annotation944896
display id: annotation944896
version: 1
top level: BBa_P0440
location: https://synbiohub.org/public/igem/BBa_P0440/annotation944896/range944896/1
component: BBa_C0040

https://synbiohub.org/public/igem/BBa_P0440/annotation944896/range944896/1
type: Range
persistent identity: range944896
display id: range944896
version: 1
top level: BBa_P0440
direction: direction/forward
start: 19
end: 678
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation944902
display id: annotation944902
version: 1
top level: BBa_P0440
location: https://synbiohub.org/public/igem/BBa_P0440/annotation944902/range944902/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_P0440/annotation944902/range944902/1
type: Range
persistent identity: range944902
display id: range944902
version: 1
top level: BBa_P0440
direction: direction/forward
start: 712
end: 791
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation944912
display id: annotation944912
version: 1
top level: BBa_P0440
location: https://synbiohub.org/public/igem/BBa_P0440/annotation944912/range944912/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_P0440/annotation944912/range944912/1
type: Range
persistent identity: range944912
display id: range944912
version: 1
top level: BBa_P0440
direction: direction/forward
start: 800
end: 840
orientation: inline

BBa_K1022101
type: ComponentDefinition
persistent identity: BBa K1022101
display id: BBa_K1022101
version: 1
was generated by: iGEM to SBOL conversion
description: pT7: RBS: His6 - SUMO: MaximinH5
created: 2013-09-04T11:00:00Z
modified: 2015-05-08T01:08:44Z
mutable provenance: Synthetic
owned by: james
owned by: myers
mutable description: This part codes for the peptide 'Maximin H5' tagged with 'His-6-SUMO' molecule. Its production is triggered by IPTG (through pT7 promoter).
discontinued: false
dominant: false
experience: experience/Works
group u list: _1329_
m user id: 0
owner id: 15691
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: -
star: false
creator: Maithili Krishnan
type: DnaRegion
role: SO:0000110
role: partType/DNA
sequence annotation: T7
sequence annotation: 34
sequence annotation: His6:SUMO
sequence annotation: MaximinH5
sequence: https://synbiohub.org/public/igem/BBa_K1022101_sequence/1

T7
type: SequenceAnnotation
persistent identity: annotation2334990
display id: annotation2334990
version: 1
top level: BBa_K1022101
location: https://synbiohub.org/public/igem/BBa_K1022101/annotation2334990/range2334990/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_K1022101/annotation2334990/range2334990/1
type: Range
persistent identity: range2334990
display id: range2334990
version: 1
top level: BBa_K1022101
direction: direction/forward
start: 1
end: 28
orientation: inline

34
type: SequenceAnnotation
persistent identity: annotation2334991
display id: annotation2334991
version: 1
top level: BBa_K1022101
location: https://synbiohub.org/public/igem/BBa_K1022101/annotation2334991/range2334991/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_K1022101/annotation2334991/range2334991/1
type: Range
persistent identity: range2334991
display id: range2334991
version: 1
top level: BBa_K1022101
direction: direction/forward
start: 29
end: 42
orientation: inline

His6:SUMO
type: SequenceAnnotation
persistent identity: annotation2334992
display id: annotation2334992
version: 1
top level: BBa_K1022101
location: https://synbiohub.org/public/igem/BBa_K1022101/annotation2334992/range2334992/1
role: SO:0000324
role: feature/tag

https://synbiohub.org/public/igem/BBa_K1022101/annotation2334992/range2334992/1
type: Range
persistent identity: range2334992
display id: range2334992
version: 1
top level: BBa_K1022101
direction: direction/forward
start: 43
end: 363
orientation: inline

MaximinH5
type: SequenceAnnotation
persistent identity: annotation2334993
display id: annotation2334993
version: 1
top level: BBa_K1022101
location: https://synbiohub.org/public/igem/BBa_K1022101/annotation2334993/range2334993/1
role: feature/dna
role: SO:0000110

https://synbiohub.org/public/igem/BBa_K1022101/annotation2334993/range2334993/1
type: Range
persistent identity: range2334993
display id: range2334993
version: 1
top level: BBa_K1022101
direction: direction/forward
start: 364
end: 427
orientation: inline

BBa_K1022109
type: ComponentDefinition
persistent identity: BBa K1022109
display id: BBa_K1022109
version: 1
was generated by: iGEM to SBOL conversion
description: Lysis device with corrected Suffix
created: 2013-09-19T11:00:00Z
modified: 2015-05-08T01:08:45Z
mutable provenance: Clonned
owned by: james
owned by: myers
mutable description: This part is an improvement of the Bio-brick Bba_K112808. It has a corrected suffix from the earlier version.
discontinued: false
dominant: false
experience: experience/Works
group u list: _1329_
m user id: 0
owner id: 15691
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: The biobrick Bba_K112808 didnot have a proper suffix behind the lysis cassette. Team Tu Delft iGEM 2013 corrected this part.
star: false
creator: Maithili Krishnan
type: DnaRegion
role: SO:0000804
role: partType/Device
sequence: https://synbiohub.org/public/igem/BBa_K1022109_sequence/1

BBa_K1022115
type: ComponentDefinition
persistent identity: BBa K1022115
display id: BBa_K1022115
version: 1
was generated by: iGEM to SBOL conversion
description: pTet: cI: TT : pcI: Ulp
created: 2013-09-19T11:00:00Z
modified: 2015-05-08T01:08:45Z
mutable provenance: Clonned
owned by: james
owned by: myers
mutable description: This biobrick codes for promoter Tet followed by cI and double terminator with promoter cI and Ulp protease gene.
discontinued: false
dominant: false
group u list: _1329_
m user id: 0
owner id: 15691
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: -
star: false
creator: Maithili Krishnan
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_K1022107
component: BBa_K1022108
sequence annotation: BBa_K1022108
sequence annotation: BBa_K1022107
sequence: https://synbiohub.org/public/igem/BBa_K1022115_sequence/1

BBa_K1022107
type: Component
persistent identity: component2356274
display id: component2356274
version: 1
top level: BBa_K1022115
definition: BBa_K1022107
access: public

BBa_K1022108
type: Component
persistent identity: component2356270
display id: component2356270
version: 1
top level: BBa_K1022115
definition: BBa_K1022108
access: public

BBa_K1022108
type: SequenceAnnotation
persistent identity: annotation2356270
display id: annotation2356270
version: 1
top level: BBa_K1022115
location: https://synbiohub.org/public/igem/BBa_K1022115/annotation2356270/range2356270/1
component: BBa_K1022108

https://synbiohub.org/public/igem/BBa_K1022115/annotation2356270/range2356270/1
type: Range
persistent identity: range2356270
display id: range2356270
version: 1
top level: BBa_K1022115
direction: direction/reverse
start: 1
end: 992
orientation: inline

BBa_K1022107
type: SequenceAnnotation
persistent identity: annotation2356274
display id: annotation2356274
version: 1
top level: BBa_K1022115
location: https://synbiohub.org/public/igem/BBa_K1022115/annotation2356274/range2356274/1
component: BBa_K1022107

https://synbiohub.org/public/igem/BBa_K1022115/annotation2356274/range2356274/1
type: Range
persistent identity: range2356274
display id: range2356274
version: 1
top level: BBa_K1022115
direction: direction/reverse
start: 1001
end: 1815
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

tetR
type: ComponentDefinition
persistent identity: BBa C0040
display id: BBa_C0040
version: 1
was generated by: iGEM to SBOL conversion
description: tetracycline repressor from transposon Tn10 (+LVA)
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:23Z
mutable provenance: Elowitz, M. B. Transport, Assembly, and Dynamics in Systems of Interacting Proteins. Thesis, Princeton Univ., Princeton (1999)
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Coding region for the TetR protein without the Ribosome Binding Site. Modified with an LVA tail for rapid degradation of the protein and faster fall time for the emission. TetR binds to the pTet regulator (BBa_R0040). aTc (anhydrotetracycline) binds to TetR and inhibits its operation.
discontinued: false
dominant: true
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: References (unparsed) here: Elowitz, M. B. Transport, Assembly, and Dynamics in Systems of Interacting Proteins. Thesis, Princeton Univ., Princeton (1999). Lutz R, Bujard H., Independent and tight regulation of transcriptional units in Escherichia coli via the LacR/O, the TetR/O and AraC/I1-I2 regulatory elements. Nucleic Acids Res. 1997 Mar 15;25(6):1203-10. PMID: 9092630 References (unparsed) here: Elowitz, M. B. Transport, Assembly, and Dynamics in Systems of Interacting Proteins. Thesis, Princeton Univ., Princeton (1999). Lutz R, Bujard H., Independent and tight regulation of transcriptional units in Escherichia coli via the LacR/O, the TetR/O and AraC/I1-I2 regulatory elements. Nucleic Acids Res. 1997 Mar 15;25(6):1203-10. PMID: 9092630 BBa_C0040 TetR Protein is based on the TetR sequence from Elowitz's repressilator. It has been modified to include a rapid degradation LVA tail, and includes the BioBrick standard assembly head and tail restriction sites. The RBS has been removed. The stop codon has been changed from TAA to a double stop codon TAATAA.
star: true
creator: June Rhee, Connie Tao, Ty Thomson, Louis Waldman.
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: tetR
sequence annotation: SsrA
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_C0040_sequence/1

tetR
type: SequenceAnnotation
persistent identity: annotation23329
display id: annotation23329
version: 1
top level: tetR
location: https://synbiohub.org/public/igem/BBa_C0040/annotation23329/range23329/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_C0040/annotation23329/range23329/1
type: Range
persistent identity: range23329
display id: range23329
version: 1
top level: tetR
direction: direction/forward
start: 4
end: 620
orientation: inline

SsrA
type: SequenceAnnotation
persistent identity: annotation23330
display id: annotation23330
version: 1
top level: tetR
location: https://synbiohub.org/public/igem/BBa_C0040/annotation23330/range23330/1
role: feature/tag
role: SO:0000324

https://synbiohub.org/public/igem/BBa_C0040/annotation23330/range23330/1
type: Range
persistent identity: range23330
display id: range23330
version: 1
top level: tetR
direction: direction/forward
start: 621
end: 654
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2213989
display id: annotation2213989
version: 1
top level: tetR
location: https://synbiohub.org/public/igem/BBa_C0040/annotation2213989/range2213989/1
role: SO:0000807
role: feature/barcode

https://synbiohub.org/public/igem/BBa_C0040/annotation2213989/range2213989/1
type: Range
persistent identity: range2213989
display id: range2213989
version: 1
top level: tetR
direction: direction/forward
start: 661
end: 685
orientation: inline

BBa_K1022107
type: ComponentDefinition
persistent identity: BBa K1022107
display id: BBa_K1022107
version: 1
was generated by: iGEM to SBOL conversion
description: pcI Ulp
created: 2013-09-04T11:00:00Z
modified: 2015-05-08T01:08:44Z
mutable provenance: Synthetic
owned by: james
owned by: myers
mutable description: This part codes for the Promoter cI and Ulp molecule.
discontinued: false
dominant: false
group u list: _1329_
m user id: 0
owner id: 15691
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: -
star: false
creator: Maithili Krishnan
type: DnaRegion
role: SO:0000110
role: partType/DNA
sequence annotation: cI
sequence annotation: 34
sequence annotation: Ulp
sequence: https://synbiohub.org/public/igem/BBa_K1022107_sequence/1

cI
type: SequenceAnnotation
persistent identity: annotation2335074
display id: annotation2335074
version: 1
top level: BBa_K1022107
location: https://synbiohub.org/public/igem/BBa_K1022107/annotation2335074/range2335074/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_K1022107/annotation2335074/range2335074/1
type: Range
persistent identity: range2335074
display id: range2335074
version: 1
top level: BBa_K1022107
direction: direction/forward
start: 1
end: 49
orientation: inline

34
type: SequenceAnnotation
persistent identity: annotation2335075
display id: annotation2335075
version: 1
top level: BBa_K1022107
location: https://synbiohub.org/public/igem/BBa_K1022107/annotation2335075/range2335075/1
role: SO:0000139
role: feature/rbs

https://synbiohub.org/public/igem/BBa_K1022107/annotation2335075/range2335075/1
type: Range
persistent identity: range2335075
display id: range2335075
version: 1
top level: BBa_K1022107
direction: direction/forward
start: 58
end: 74
orientation: inline

Ulp
type: SequenceAnnotation
persistent identity: annotation2335076
display id: annotation2335076
version: 1
top level: BBa_K1022107
location: https://synbiohub.org/public/igem/BBa_K1022107/annotation2335076/range2335076/1
role: SO:0000110
role: feature/dna

https://synbiohub.org/public/igem/BBa_K1022107/annotation2335076/range2335076/1
type: Range
persistent identity: range2335076
display id: range2335076
version: 1
top level: BBa_K1022107
direction: direction/forward
start: 75
end: 815
orientation: inline

BBa_K1022108
type: ComponentDefinition
persistent identity: BBa K1022108
display id: BBa_K1022108
version: 1
was generated by: iGEM to SBOL conversion
description: pTet: cI: TT
created: 2013-09-04T11:00:00Z
modified: 2015-05-08T01:08:45Z
mutable provenance: Sythetic
owned by: james
owned by: myers
mutable description: This parts codes for promoter Tet, cI and has a double terminator.
discontinued: false
dominant: false
group u list: _1329_
m user id: 0
owner id: 15691
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: -
star: false
creator: Maithili Krishnan
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_J13002
component: BBa_B0015
component: BBa_C0051
sequence annotation: BBa_J13002
sequence annotation: BBa_C0051
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_K1022108_sequence/1

BBa_J13002
type: Component
persistent identity: component2335084
display id: component2335084
version: 1
top level: BBa_K1022108
definition: BBa_J13002
access: public

BBa_B0015
type: Component
persistent identity: component2335095
display id: component2335095
version: 1
top level: BBa_K1022108
definition: BBa_B0015
access: public

BBa_C0051
type: Component
persistent identity: component2335088
display id: component2335088
version: 1
top level: BBa_K1022108
definition: cI lam
access: public

BBa_J13002
type: SequenceAnnotation
persistent identity: annotation2335084
display id: annotation2335084
version: 1
top level: BBa_K1022108
location: https://synbiohub.org/public/igem/BBa_K1022108/annotation2335084/range2335084/1
component: BBa_J13002

https://synbiohub.org/public/igem/BBa_K1022108/annotation2335084/range2335084/1
type: Range
persistent identity: range2335084
display id: range2335084
version: 1
top level: BBa_K1022108
direction: direction/reverse
start: 1
end: 74
orientation: inline

BBa_C0051
type: SequenceAnnotation
persistent identity: annotation2335088
display id: annotation2335088
version: 1
top level: BBa_K1022108
location: https://synbiohub.org/public/igem/BBa_K1022108/annotation2335088/range2335088/1
component: BBa_C0051

https://synbiohub.org/public/igem/BBa_K1022108/annotation2335088/range2335088/1
type: Range
persistent identity: range2335088
display id: range2335088
version: 1
top level: BBa_K1022108
direction: direction/reverse
start: 81
end: 855
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2335095
display id: annotation2335095
version: 1
top level: BBa_K1022108
location: https://synbiohub.org/public/igem/BBa_K1022108/annotation2335095/range2335095/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K1022108/annotation2335095/range2335095/1
type: Range
persistent identity: range2335095
display id: range2335095
version: 1
top level: BBa_K1022108
direction: direction/reverse
start: 864
end: 992
orientation: inline

BBa_B0015
type: ComponentDefinition
persistent identity: BBa B0015
display id: BBa_B0015
version: 1
was generated by: iGEM to SBOL conversion
description: double terminator (B0010-B0012)
created: 2003-07-16T11:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Double terminator consisting of BBa_B0010 and BBa_B0012
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Reshma Shetty
type: DnaRegion
role: partType/Terminator
role: SO:0000141
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_B0015_sequence/1

BBa_B0012
type: Component
persistent identity: component1916612
display id: component1916612
version: 1
top level: BBa_B0015
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1916610
display id: component1916610
version: 1
top level: BBa_B0015
definition: BBa_B0010
access: public

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1916610
display id: annotation1916610
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
type: Range
persistent identity: range1916610
display id: range1916610
version: 1
top level: BBa_B0015
direction: direction/forward
start: 1
end: 80
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1916612
display id: annotation1916612
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
type: Range
persistent identity: range1916612
display id: range1916612
version: 1
top level: BBa_B0015
direction: direction/forward
start: 89
end: 129
orientation: inline

BBa_J13002
type: ComponentDefinition
persistent identity: BBa J13002
display id: BBa_J13002
version: 1
was generated by: iGEM to SBOL conversion
description: TetR repressed POPS/RIPS generator
created: 2005-06-15T11:00:00Z
modified: 2015-08-31T04:08:29Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: -- No description --
discontinued: false
dominant: true
experience: experience/Works
group u list: _37_5_
m user id: 0
owner id: 88
owning group id: 37
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Jeff Tabor
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
component: BBa_B0034
component: BBa_R0040
sequence annotation: BBa_R0040
sequence annotation: BBa_B0034
sequence: https://synbiohub.org/public/igem/BBa_J13002_sequence/1

BBa_B0034
type: Component
persistent identity: component1535786
display id: component1535786
version: 1
top level: BBa_J13002
definition: BBa_B0034
access: public

BBa_R0040
type: Component
persistent identity: component1535778
display id: component1535778
version: 1
top level: BBa_J13002
definition: p(tetR)
access: public

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation1535778
display id: annotation1535778
version: 1
top level: BBa_J13002
location: https://synbiohub.org/public/igem/BBa_J13002/annotation1535778/range1535778/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_J13002/annotation1535778/range1535778/1
type: Range
persistent identity: range1535778
display id: range1535778
version: 1
top level: BBa_J13002
direction: direction/forward
start: 1
end: 54
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1535786
display id: annotation1535786
version: 1
top level: BBa_J13002
location: https://synbiohub.org/public/igem/BBa_J13002/annotation1535786/range1535786/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_J13002/annotation1535786/range1535786/1
type: Range
persistent identity: range1535786
display id: range1535786
version: 1
top level: BBa_J13002
direction: direction/forward
start: 63
end: 74
orientation: inline

cI lam
type: ComponentDefinition
persistent identity: BBa C0051
display id: BBa_C0051
version: 1
was generated by: iGEM to SBOL conversion
description: cI repressor from E. coli phage lambda (+LVA)
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:23Z
mutable provenance: Elowitz, M. B. Transport, Assembly, and Dynamics in Systems of Interacting Proteins. Thesis, Princeton Univ., Princeton (1999).
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Coding region for the cI repressor based on cI repressor from bacteriophage lambda modified with an LVA tail for rapid degradation of the protein. cI repressor binds to the cI regulator (BBa_R0051).
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: References (unparsed) here: A synthetic oscillatory network of transcriptional regulators , Elowitz M.B. , Leibler S., Nature(403),335-38: 2000 Octamerization of CI repressor is needed for effective repression of PRM and efficient switching from lysogeny. Ian B. Dodd,1 Alison J. Perkins, Daniel Tsemitsidis, and J. Barry Egan , Genes and Development (Vol 15, No. 22) 3013-3022: 2001 References (unparsed) here: A synthetic oscillatory network of transcriptional regulators , Elowitz M.B. , Leibler S., Nature(403),335-38: 2000 Octamerization of CI repressor is needed for effective repression of PRM and efficient switching from lysogeny. Ian B. Dodd,1 Alison J. Perkins, Daniel Tsemitsidis, and J. Barry Egan , Genes and Development (Vol 15, No. 22) 3013-3022: 2001 BBa_C0051 cI repressor is based on the cI repressor from the Elowitz's repressilator. It has been modified to include a rapid degradation LAA tail, and includes the BioBrick standard assembly head and tail restriction sites. The RBS has been removed. The stop codon has been changed from TAA to a double stop codon TAATAA.
star: true
creator: Vinay S Mahajan, Brian Chow, Peter Carr, Voichita Marinescu and Alexander D. Wissner-Gross
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: cI lambda
sequence annotation: LVA
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_C0051_sequence/1

cI lambda
type: SequenceAnnotation
persistent identity: annotation23334
display id: annotation23334
version: 1
top level: cI lam
location: https://synbiohub.org/public/igem/BBa_C0051/annotation23334/range23334/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_C0051/annotation23334/range23334/1
type: Range
persistent identity: range23334
display id: range23334
version: 1
top level: cI lam
direction: direction/forward
start: 4
end: 711
orientation: inline

LVA
type: SequenceAnnotation
persistent identity: annotation23335
display id: annotation23335
version: 1
top level: cI lam
location: https://synbiohub.org/public/igem/BBa_C0051/annotation23335/range23335/1
role: SO:0000324
role: feature/tag

https://synbiohub.org/public/igem/BBa_C0051/annotation23335/range23335/1
type: Range
persistent identity: range23335
display id: range23335
version: 1
top level: cI lam
direction: direction/forward
start: 712
end: 744
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2213991
display id: annotation2213991
version: 1
top level: cI lam
location: https://synbiohub.org/public/igem/BBa_C0051/annotation2213991/range2213991/1
role: feature/barcode
role: SO:0000807

https://synbiohub.org/public/igem/BBa_C0051/annotation2213991/range2213991/1
type: Range
persistent identity: range2213991
display id: range2213991
version: 1
top level: cI lam
direction: direction/forward
start: 751
end: 775
orientation: inline

p(tetR)
type: ComponentDefinition
persistent identity: BBa R0040
display id: BBa_R0040
version: 1
was generated by: iGEM to SBOL conversion
description: TetR repressible promoter
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: Lutz, R., Bujard, H., Nucleic Acids Research (1997) 25, 1203-1210.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Sequence for pTet inverting regulator driven by the TetR protein.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: BBa_R0040 TetR-Regulated Promoter is based on a cI promoter. It has been modified to include two TetR binding sites and the BioBrick standard assembly head and tail restriction sites.
star: true
creator: June Rhee, Connie Tao, Ty Thomson, Louis Waldman
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: BBa_R0040
sequence annotation: TetR 1
sequence annotation: -35
sequence annotation: TetR 2
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_R0040_sequence/1

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation1986784
display id: annotation1986784
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986784/range1986784/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_R0040/annotation1986784/range1986784/1
type: Range
persistent identity: range1986784
display id: range1986784
version: 1
top level: p(tetR)
direction: direction/forward
start: 1
end: 54
orientation: inline

TetR 1
type: SequenceAnnotation
persistent identity: annotation1986783
display id: annotation1986783
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986783/range1986783/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0040/annotation1986783/range1986783/1
type: Range
persistent identity: range1986783
display id: range1986783
version: 1
top level: p(tetR)
direction: direction/forward
start: 1
end: 19
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1986785
display id: annotation1986785
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986785/range1986785/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0040/annotation1986785/range1986785/1
type: Range
persistent identity: range1986785
display id: range1986785
version: 1
top level: p(tetR)
direction: direction/forward
start: 20
end: 25
orientation: inline

TetR 2
type: SequenceAnnotation
persistent identity: annotation1986786
display id: annotation1986786
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986786/range1986786/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_R0040/annotation1986786/range1986786/1
type: Range
persistent identity: range1986786
display id: range1986786
version: 1
top level: p(tetR)
direction: direction/forward
start: 26
end: 44
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1986787
display id: annotation1986787
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986787/range1986787/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0040/annotation1986787/range1986787/1
type: Range
persistent identity: range1986787
display id: range1986787
version: 1
top level: p(tetR)
direction: direction/forward
start: 43
end: 48
orientation: inline

https://synbiohub.org/public/igem/BBa_K1022124_sequence/1
type: Sequence
persistent identity: BBa K1022124 sequence
display id: BBa_K1022124_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: taatacgactcactatagggagagaaagaggagaaatactagatggctagcatgcatcatcatcatcatcattcggactcagaagtcaatcaagaagctaagccagaggtcaagccagaagtcaagcctgagactcacatcaatttaaaggtgtccgatggatcttcagagatcttcttcaagatcaaaaagaccactcctttaagaaggctgatggaagcgttcgctaaacgtcagggtaaggaaatggactccttaagattcttgtacgacggtattaggattcaagctgatcagacccctgaagatttggacatggatgataacgatattattgaggctcacagagaacagaccggtggtatcctgggtccggttctgggtctggtttctgacaccctggacgacgttctgggtatcctgtaattactagagaaagaggagaaatactagatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatactagatgagcacaaaaaagaaaccattaacacaagagcagcttgaggacgcacgtcgccttaaagcaatttatgaaaaaaagaaaaatgaacttggcttatcccaggaatctgtcgcagacaagatggggatggggcagtcaggcgttggtgctttatttaatggcatcaatgcattaaatgcttataacgccgcattgcttgcaaaaattctcaaagttagcgttgaagaatttagcccttcaatcgccagagaaatctacgagatgtatgaagcggttagtatgcagccgtcacttagaagtgagtatgagtaccctgttttttctcatgttcaggcagggatgttctcacctgagcttagaacctttaccaaaggtgatgcggagagatgggtaagcacaaccaaaaaagccagtgattctgcattctggcttgaggttgaaggtaattccatgaccgcaccaacaggctccaagccaagctttcctgacggaatgttaattctcgttgaccctgagcaggctgttgagccaggtgatttctgcatagccagacttgggggtgatgagtttaccttcaagaaactgatcagggatagcggtcaggtgtttttacaaccactaaacccacagtacccaa…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1022104_sequence/1
type: Sequence
persistent identity: BBa K1022104 sequence
display id: BBa_K1022104_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: taatacgactcactatagggagagaaagaggagaaatactagatggctagcatgcatcatcatcatcatcattcggactcagaagtcaatcaagaagctaagccagaggtcaagccagaagtcaagcctgagactcacatcaatttaaaggtgtccgatggatcttcagagatcttcttcaagatcaaaaagaccactcctttaagaaggctgatggaagcgttcgctaaacgtcagggtaaggaaatggactccttaagattcttgtacgacggtattaggattcaagctgatcagacccctgaagatttggacatggatgataacgatattattgaggctcacagagaacagaccggtggtatcctgggtccggttctgggtctggtttctgacaccctggacgacgttctgggtatcctgtaattactagagaaagaggagaaatactagatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1022123_sequence/1
type: Sequence
persistent identity: BBa K1022123 sequence
display id: BBa_K1022123_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatactagatgagcacaaaaaagaaaccattaacacaagagcagcttgaggacgcacgtcgccttaaagcaatttatgaaaaaaagaaaaatgaacttggcttatcccaggaatctgtcgcagacaagatggggatggggcagtcaggcgttggtgctttatttaatggcatcaatgcattaaatgcttataacgccgcattgcttgcaaaaattctcaaagttagcgttgaagaatttagcccttcaatcgccagagaaatctacgagatgtatgaagcggttagtatgcagccgtcacttagaagtgagtatgagtaccctgttttttctcatgttcaggcagggatgttctcacctgagcttagaacctttaccaaaggtgatgcggagagatgggtaagcacaaccaaaaaagccagtgattctgcattctggcttgaggttgaaggtaattccatgaccgcaccaacaggctccaagccaagctttcctgacggaatgttaattctcgttgaccctgagcaggctgttgagccaggtgatttctgcatagccagacttgggggtgatgagtttaccttcaagaaactgatcagggatagcggtcaggtgtttttacaaccactaaacccacagtacccaatgatcccatgcaatgagagttgttccgttgtggggaaagttatcgctagtcagtggcctgaagagacgtttggcgctgcaaacgacgaaaactacgctttagtagcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtaacaccgtgcgtgttgactattttacctctggcggtgataatggttgcttaccggaaagaggagaaatactagatggctagccttgttcctgaattaaatgaaaaagacgatgaccaagtacaaaaagctttggcatcgagagaaaatactcagttaatgaatagagataatatagagataacagtacgtgattttaagaccttggcaccacgaagatggctaaatgacactatcattgagttttttatgaaatacattgaaaaatctacccctaatacagtggcgtttaattcatttttctataccaatttatcagaaaggggttatcaaggcgtccggaggtggatgaagagaaagaagacacaaattgataaacttgataaaatctttacaccaataaatttgaaccaatcccactgggcgttgggcataattgatttaaaaaagaaaactataggttacgtagattcattatcgaatggtccaaatgctatgagtttcgctatactgactgacttgcaaaaatatgttatggaggaaagtaagcatacaataggagaagactttgatttgattcatttagattgtccgcagcaaccaaatggctacgactgtggaatatatgtttgtatgaatactctctatggaagtgcagatgcgccattggattttgattataaagatgcgattaggatgagaagatttattgcccatttgattttaaccgacgctttaaaacgggatccgaactcgagctccgtcgacaagcttgcggccgcactcgagcaccaccaccaccaccactgagtacattactagagcttaaaaggagggtctatggcagcacctagaatatcattttcgccctctgatattctatttggtgttctcgatcgcttgttcaaagataacgctaccgggaaggttcttgcttcccgggtagctgtcgtaattcttttgtttataatggcgattgtttggtataggggagatagttt…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_P0440_sequence/1
type: Sequence
persistent identity: BBa P0440 sequence
display id: BBa_P0440_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1022101_sequence/1
type: Sequence
persistent identity: BBa K1022101 sequence
display id: BBa_K1022101_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: taatacgactcactatagggagagaaagaggagaaatactagatggctagcatgcatcatcatcatcatcattcggactcagaagtcaatcaagaagctaagccagaggtcaagccagaagtcaagcctgagactcacatcaatttaaaggtgtccgatggatcttcagagatcttcttcaagatcaaaaagaccactcctttaagaaggctgatggaagcgttcgctaaacgtcagggtaaggaaatggactccttaagattcttgtacgacggtattaggattcaagctgatcagacccctgaagatttggacatggatgataacgatattattgaggctcacagagaacagaccggtggtatcctgggtccggttctgggtctggtttctgacaccctggacgacgttctgggtatcctgtaat
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1022109_sequence/1
type: Sequence
persistent identity: BBa K1022109 sequence
display id: BBa_K1022109_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: cttaaaaggagggtctatggcagcacctagaatatcattttcgccctctgatattctatttggtgttctcgatcgcttgttcaaagataacgctaccgggaaggttcttgcttcccgggtagctgtcgtaattcttttgtttataatggcgattgtttggtataggggagatagtttctttgagtactataagcaatcaaagtatgaaacatacagtgaaattattgaaaaggaaagaactgcacgctttgaatctgtcgccctggaacaactccagatagttcatatatcatctgaggcagactttagtgcggtgtattctttccgccctaaaaacttaaactattttgttgatattatagcatacgaaggaaaattaccttcaacaataagtgaaaaatcacttggaggatatcctgttgataaaactatggatgaatatacagttcatttaaatggacgtcattattattccaactcaaaatttgcttttttaccaactaaaaagcctactcccgaaataaactacatgtacagttgtccatattttaatttggataatatctatgctggaacgataaccatgtactggtatagaaatgatcatataagtaatgaccgccttgaatcaatatgtgctcaggcggccagaatattaggaagggctaaataattatactagagatacttaggaggtattatgaatatatttgaaatgttacgtatagatgaaggtcttagacttaaaatctataaagacacagaaggctattacactattggcatcggtcatttgcttacaaaaagtccatcacttaatgctgctaaatctgaattagataaagctattgggcgtaattgcaatggtgtaattacaaaagatgaggctgaaaaactctttaatcaggatgttgatgctgctgttcgcggaatcctgagaaatgctaaattaaaaccggtttatgattctcttgatgcggttcgtcgctgtgcattgattaatatggttttccaaatgggagaaaccggtgtggcaggatttactaactctttacgtatgcttcaacaaaaacgctgggatgaagcagcagttaacttagctaaaagtagatggtataatcaaacacctaatcgcgcaaaacgagtcattacaacgtttagaactggcacttgggacgcgtataaaaatctataaagctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagttgacagctagctcagtcctagggactatgctagctactagaggataggaggcctttatggccttaaaagcaacagcactttttgccatgctaggattgtcatttgttttatctccatcgattgaagcgaatgtcgatcctcattttgataaatttatggaatctggtattaggcacgtttatatgctttttgaaaataaaagcgtagaatcgtctgaacaattctatagttttatgagaacgacctataaaaatgacccgtgctcttctgattttgaatgtatagagcgaggcgcggagatggcacaatcatacgctagaattatgaacattaaattggagactgaatgaaattactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1022115_sequence/1
type: Sequence
persistent identity: BBa K1022115 sequence
display id: BBa_K1022115_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatactagatgagcacaaaaaagaaaccattaacacaagagcagcttgaggacgcacgtcgccttaaagcaatttatgaaaaaaagaaaaatgaacttggcttatcccaggaatctgtcgcagacaagatggggatggggcagtcaggcgttggtgctttatttaatggcatcaatgcattaaatgcttataacgccgcattgcttgcaaaaattctcaaagttagcgttgaagaatttagcccttcaatcgccagagaaatctacgagatgtatgaagcggttagtatgcagccgtcacttagaagtgagtatgagtaccctgttttttctcatgttcaggcagggatgttctcacctgagcttagaacctttaccaaaggtgatgcggagagatgggtaagcacaaccaaaaaagccagtgattctgcattctggcttgaggttgaaggtaattccatgaccgcaccaacaggctccaagccaagctttcctgacggaatgttaattctcgttgaccctgagcaggctgttgagccaggtgatttctgcatagccagacttgggggtgatgagtttaccttcaagaaactgatcagggatagcggtcaggtgtttttacaaccactaaacccacagtacccaatgatcccatgcaatgagagttgttccgttgtggggaaagttatcgctagtcagtggcctgaagagacgtttggcgctgcaaacgacgaaaactacgctttagtagcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtaacaccgtgcgtgttgactattttacctctggcggtgataatggttgcttaccggaaagaggagaaatactagatggctagccttgttcctgaattaaatgaaaaagacgatgaccaagtacaaaaagctttggcatcgagagaaaatactcagttaatgaatagagataatatagagataacagtacgtgattttaagaccttggcaccacgaagatggctaaatgacactatcattgagttttttatgaaatacattgaaaaatctacccctaatacagtggcgtttaattcatttttctataccaatttatcagaaaggggttatcaaggcgtccggaggtggatgaagagaaagaagacacaaattgataaacttgataaaatctttacaccaataaatttgaaccaatcccactgggcgttgggcataattgatttaaaaaagaaaactataggttacgtagattcattatcgaatggtccaaatgctatgagtttcgctatactgactgacttgcaaaaatatgttatggaggaaagtaagcatacaataggagaagactttgatttgattcatttagattgtccgcagcaaccaaatggctacgactgtggaatatatgtttgtatgaatactctctatggaagtgcagatgcgccattggattttgattataaagatgcgattaggatgagaagatttattgcccatttgattttaaccgacgctttaaaacgggatccgaactcgagctccgtcgacaagcttgcggccgcactcgagcaccaccaccaccaccactgagtacat
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_C0040_sequence/1
type: Sequence
persistent identity: BBa C0040 sequence
display id: BBa_C0040_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1022107_sequence/1
type: Sequence
persistent identity: BBa K1022107 sequence
display id: BBa_K1022107_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: taacaccgtgcgtgttgactattttacctctggcggtgataatggttgcttaccggaaagaggagaaatactagatggctagccttgttcctgaattaaatgaaaaagacgatgaccaagtacaaaaagctttggcatcgagagaaaatactcagttaatgaatagagataatatagagataacagtacgtgattttaagaccttggcaccacgaagatggctaaatgacactatcattgagttttttatgaaatacattgaaaaatctacccctaatacagtggcgtttaattcatttttctataccaatttatcagaaaggggttatcaaggcgtccggaggtggatgaagagaaagaagacacaaattgataaacttgataaaatctttacaccaataaatttgaaccaatcccactgggcgttgggcataattgatttaaaaaagaaaactataggttacgtagattcattatcgaatggtccaaatgctatgagtttcgctatactgactgacttgcaaaaatatgttatggaggaaagtaagcatacaataggagaagactttgatttgattcatttagattgtccgcagcaaccaaatggctacgactgtggaatatatgtttgtatgaatactctctatggaagtgcagatgcgccattggattttgattataaagatgcgattaggatgagaagatttattgcccatttgattttaaccgacgctttaaaacgggatccgaactcgagctccgtcgacaagcttgcggccgcactcgagcaccaccaccaccaccactgagtacat
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1022108_sequence/1
type: Sequence
persistent identity: BBa K1022108 sequence
display id: BBa_K1022108_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatactagatgagcacaaaaaagaaaccattaacacaagagcagcttgaggacgcacgtcgccttaaagcaatttatgaaaaaaagaaaaatgaacttggcttatcccaggaatctgtcgcagacaagatggggatggggcagtcaggcgttggtgctttatttaatggcatcaatgcattaaatgcttataacgccgcattgcttgcaaaaattctcaaagttagcgttgaagaatttagcccttcaatcgccagagaaatctacgagatgtatgaagcggttagtatgcagccgtcacttagaagtgagtatgagtaccctgttttttctcatgttcaggcagggatgttctcacctgagcttagaacctttaccaaaggtgatgcggagagatgggtaagcacaaccaaaaaagccagtgattctgcattctggcttgaggttgaaggtaattccatgaccgcaccaacaggctccaagccaagctttcctgacggaatgttaattctcgttgaccctgagcaggctgttgagccaggtgatttctgcatagccagacttgggggtgatgagtttaccttcaagaaactgatcagggatagcggtcaggtgtttttacaaccactaaacccacagtacccaatgatcccatgcaatgagagttgttccgttgtggggaaagttatcgctagtcagtggcctgaagagacgtttggcgctgcaaacgacgaaaactacgctttagtagcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0015_sequence/1
type: Sequence
persistent identity: BBa B0015 sequence
display id: BBa_B0015_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J13002_sequence/1
type: Sequence
persistent identity: BBa J13002 sequence
display id: BBa_J13002_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_C0051_sequence/1
type: Sequence
persistent identity: BBa C0051 sequence
display id: BBa_C0051_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgagcacaaaaaagaaaccattaacacaagagcagcttgaggacgcacgtcgccttaaagcaatttatgaaaaaaagaaaaatgaacttggcttatcccaggaatctgtcgcagacaagatggggatggggcagtcaggcgttggtgctttatttaatggcatcaatgcattaaatgcttataacgccgcattgcttgcaaaaattctcaaagttagcgttgaagaatttagcccttcaatcgccagagaaatctacgagatgtatgaagcggttagtatgcagccgtcacttagaagtgagtatgagtaccctgttttttctcatgttcaggcagggatgttctcacctgagcttagaacctttaccaaaggtgatgcggagagatgggtaagcacaaccaaaaaagccagtgattctgcattctggcttgaggttgaaggtaattccatgaccgcaccaacaggctccaagccaagctttcctgacggaatgttaattctcgttgaccctgagcaggctgttgagccaggtgatttctgcatagccagacttgggggtgatgagtttaccttcaagaaactgatcagggatagcggtcaggtgtttttacaaccactaaacccacagtacccaatgatcccatgcaatgagagttgttccgttgtggggaaagttatcgctagtcagtggcctgaagagacgtttggcgctgcaaacgacgaaaactacgctttagtagcttaataacgctgatagtgctagtgtagatcgc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0040_sequence/1
type: Sequence
persistent identity: BBa R0040 sequence
display id: BBa_R0040_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcac
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: Chris J. Myers
creator: James Alastair McLaughlin
ended at time: 2017-03-06T15:00:00.000Z