LOCUS BBa_K1022125 4876 bp DNA linear 22-Dec-2024 DEFINITION pT7: RBS: His6- SUMO: Signiferin: RBS: TetR:TT : pTet: cI: TT : pcI: Ulp : Lysis ACCESSION BBa_K1022125 VERSION BBa_K1022125.1 FEATURES Location/Qualifiers promoter 1..28 /label=T7 RBS 29..42 /label=34 misc_feature 43..363 /label=His6:SUMO misc_feature 364..412 /label=Signiferin misc_feature 1..412 /label=BBa_K1022102 misc_feature 425..428 /label=conserved RBS 421..432 /label=BBa_B0034 CDS 442..1058 /label=tetR misc_feature 1059..1092 /label=SsrA misc_feature 1099..1123 /label=Help:Barcodes CDS 439..1098 /label=BBa_C0040 stem_loop 1143..1186 /label=stem_loop misc_feature 1132..1211 /label=BBa_B0010 terminator 1132..1211 /label=BBa_B0010 stem_loop 1227..1246 /label=T7 TE misc_feature 1253..1253 /label=stop polyA_site 1247..1260 /label=polya misc_feature 1220..1260 /label=BBa_B0012 terminator 1220..1260 /label=BBa_B0012 misc_feature 421..1260 /label=BBa_P0440 misc_feature 1..1260 /label=BBa_K1022105 misc_feature 1269..1287 /label=TetR 1 misc_feature 1269..1322 /label=BBa_R0040 promoter 1288..1293 /label=-35 misc_feature 1294..1312 /label=TetR 2 promoter 1311..1316 /label=-10 promoter 1269..1322 /label=BBa_R0040 misc_feature 1335..1338 /label=conserved RBS 1331..1342 /label=BBa_B0034 promoter 1269..1342 /label=BBa_J13002 CDS 1352..2059 /label=cI lambda misc_feature 2060..2092 /label=LVA misc_feature 2099..2123 /label=Help:Barcodes CDS 1349..2123 /label=BBa_C0051 stem_loop 2143..2186 /label=stem_loop misc_feature 2132..2211 /label=BBa_B0010 terminator 2132..2211 /label=BBa_B0010 stem_loop 2227..2246 /label=T7 TE misc_feature 2253..2253 /label=stop polyA_site 2247..2260 /label=polya misc_feature 2220..2260 /label=BBa_B0012 terminator 2220..2260 /label=BBa_B0012 terminator 2132..2260 /label=BBa_B0015 misc_feature 1269..2260 /label=BBa_K1022108 promoter 2269..2317 /label=cI RBS 2326..2342 /label=34 misc_feature 2343..3083 /label=Ulp misc_feature 2269..3083 /label=BBa_K1022107 misc_feature 1269..3083 /label=BBa_K1022115 misc_feature 3092..4876 /label=BBa_K1022109 misc_feature 1269..4876 /label=BBa_K1022123 ORIGIN 1 taatacgact cactataggg agagaaagag gagaaatact agatggctag catgcatcat 61 catcatcatc attcggactc agaagtcaat caagaagcta agccagaggt caagccagaa 121 gtcaagcctg agactcacat caatttaaag gtgtccgatg gatcttcaga gatcttcttc 181 aagatcaaaa agaccactcc tttaagaagg ctgatggaag cgttcgctaa acgtcagggt 241 aaggaaatgg actccttaag attcttgtac gacggtatta ggattcaagc tgatcagacc 301 cctgaagatt tggacatgga tgataacgat attattgagg ctcacagaga acagaccggt 361 ggtatcatcg gtcacctgat caaaaccgcg ctgggtatgc tgggtctgta attactagag 421 aaagaggaga aatactagat gtccagatta gataaaagta aagtgattaa cagcgcatta 481 gagctgctta atgaggtcgg aatcgaaggt ttaacaaccc gtaaactcgc ccagaagcta 541 ggtgtagagc agcctacatt gtattggcat gtaaaaaata agcgggcttt gctcgacgcc 601 ttagccattg agatgttaga taggcaccat actcactttt gccctttaga aggggaaagc 661 tggcaagatt ttttacgtaa taacgctaaa agttttagat gtgctttact aagtcatcgc 721 gatggagcaa aagtacattt aggtacacgg cctacagaaa aacagtatga aactctcgaa 781 aatcaattag cctttttatg ccaacaaggt ttttcactag agaatgcatt atatgcactc 841 agcgctgtgg ggcattttac tttaggttgc gtattggaag atcaagagca tcaagtcgct 901 aaagaagaaa gggaaacacc tactactgat agtatgccgc cattattacg acaagctatc 961 gaattatttg atcaccaagg tgcagagcca gccttcttat tcggccttga attgatcata 1021 tgcggattag aaaaacaact taaatgtgaa agtgggtccg ctgcaaacga cgaaaactac 1081 gctttagtag cttaataaca ctgatagtgc tagtgtagat cactactaga gccaggcatc 1141 aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg 1201 tgaacgctct ctactagagt cacactggct caccttcggg tgggcctttc tgcgtttata 1261 tactagagtc cctatcagtg atagagattg acatccctat cagtgataga gatactgagc 1321 actactagag aaagaggaga aatactagat gagcacaaaa aagaaaccat taacacaaga 1381 gcagcttgag gacgcacgtc gccttaaagc aatttatgaa aaaaagaaaa atgaacttgg 1441 cttatcccag gaatctgtcg cagacaagat ggggatgggg cagtcaggcg ttggtgcttt 1501 atttaatggc atcaatgcat taaatgctta taacgccgca ttgcttgcaa aaattctcaa 1561 agttagcgtt gaagaattta gcccttcaat cgccagagaa atctacgaga tgtatgaagc 1621 ggttagtatg cagccgtcac ttagaagtga gtatgagtac cctgtttttt ctcatgttca 1681 ggcagggatg ttctcacctg agcttagaac ctttaccaaa ggtgatgcgg agagatgggt 1741 aagcacaacc aaaaaagcca gtgattctgc attctggctt gaggttgaag gtaattccat 1801 gaccgcacca acaggctcca agccaagctt tcctgacgga atgttaattc tcgttgaccc 1861 tgagcaggct gttgagccag gtgatttctg catagccaga cttgggggtg atgagtttac 1921 cttcaagaaa ctgatcaggg atagcggtca ggtgttttta caaccactaa acccacagta 1981 cccaatgatc ccatgcaatg agagttgttc cgttgtgggg aaagttatcg ctagtcagtg 2041 gcctgaagag acgtttggcg ctgcaaacga cgaaaactac gctttagtag cttaataacg 2101 ctgatagtgc tagtgtagat cgctactaga gccaggcatc aaataaaacg aaaggctcag 2161 tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct ctactagagt 2221 cacactggct caccttcggg tgggcctttc tgcgtttata tactagagta acaccgtgcg 2281 tgttgactat tttacctctg gcggtgataa tggttgctta ccggaaagag gagaaatact 2341 agatggctag ccttgttcct gaattaaatg aaaaagacga tgaccaagta caaaaagctt 2401 tggcatcgag agaaaatact cagttaatga atagagataa tatagagata acagtacgtg 2461 attttaagac cttggcacca cgaagatggc taaatgacac tatcattgag ttttttatga 2521 aatacattga aaaatctacc cctaatacag tggcgtttaa ttcatttttc tataccaatt 2581 tatcagaaag gggttatcaa ggcgtccgga ggtggatgaa gagaaagaag acacaaattg 2641 ataaacttga taaaatcttt acaccaataa atttgaacca atcccactgg gcgttgggca 2701 taattgattt aaaaaagaaa actataggtt acgtagattc attatcgaat ggtccaaatg 2761 ctatgagttt cgctatactg actgacttgc aaaaatatgt tatggaggaa agtaagcata 2821 caataggaga agactttgat ttgattcatt tagattgtcc gcagcaacca aatggctacg 2881 actgtggaat atatgtttgt atgaatactc tctatggaag tgcagatgcg ccattggatt 2941 ttgattataa agatgcgatt aggatgagaa gatttattgc ccatttgatt ttaaccgacg 3001 ctttaaaacg ggatccgaac tcgagctccg tcgacaagct tgcggccgca ctcgagcacc 3061 accaccacca ccactgagta cattactaga gcttaaaagg agggtctatg gcagcaccta 3121 gaatatcatt ttcgccctct gatattctat ttggtgttct cgatcgcttg ttcaaagata 3181 acgctaccgg gaaggttctt gcttcccggg tagctgtcgt aattcttttg tttataatgg 3241 cgattgtttg gtatagggga gatagtttct ttgagtacta taagcaatca aagtatgaaa 3301 catacagtga aattattgaa aaggaaagaa ctgcacgctt tgaatctgtc gccctggaac 3361 aactccagat agttcatata tcatctgagg cagactttag tgcggtgtat tctttccgcc 3421 ctaaaaactt aaactatttt gttgatatta tagcatacga aggaaaatta ccttcaacaa 3481 taagtgaaaa atcacttgga ggatatcctg ttgataaaac tatggatgaa tatacagttc 3541 atttaaatgg acgtcattat tattccaact caaaatttgc ttttttacca actaaaaagc 3601 ctactcccga aataaactac atgtacagtt gtccatattt taatttggat aatatctatg 3661 ctggaacgat aaccatgtac tggtatagaa atgatcatat aagtaatgac cgccttgaat 3721 caatatgtgc tcaggcggcc agaatattag gaagggctaa ataattatac tagagatact 3781 taggaggtat tatgaatata tttgaaatgt tacgtataga tgaaggtctt agacttaaaa 3841 tctataaaga cacagaaggc tattacacta ttggcatcgg tcatttgctt acaaaaagtc 3901 catcacttaa tgctgctaaa tctgaattag ataaagctat tgggcgtaat tgcaatggtg 3961 taattacaaa agatgaggct gaaaaactct ttaatcagga tgttgatgct gctgttcgcg 4021 gaatcctgag aaatgctaaa ttaaaaccgg tttatgattc tcttgatgcg gttcgtcgct 4081 gtgcattgat taatatggtt ttccaaatgg gagaaaccgg tgtggcagga tttactaact 4141 ctttacgtat gcttcaacaa aaacgctggg atgaagcagc agttaactta gctaaaagta 4201 gatggtataa tcaaacacct aatcgcgcaa aacgagtcat tacaacgttt agaactggca 4261 cttgggacgc gtataaaaat ctataaagct actagagcca ggcatcaaat aaaacgaaag 4321 gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac 4381 tagagtcaca ctggctcacc ttcgggtggg cctttctgcg tttatatact agagttgaca 4441 gctagctcag tcctagggac tatgctagct actagaggat aggaggcctt tatggcctta 4501 aaagcaacag cactttttgc catgctagga ttgtcatttg ttttatctcc atcgattgaa 4561 gcgaatgtcg atcctcattt tgataaattt atggaatctg gtattaggca cgtttatatg 4621 ctttttgaaa ataaaagcgt agaatcgtct gaacaattct atagttttat gagaacgacc 4681 tataaaaatg acccgtgctc ttctgatttt gaatgtatag agcgaggcgc ggagatggca 4741 caatcatacg ctagaattat gaacattaaa ttggagactg aatgaaatta ctagagccag 4801 gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt 4861 gtcggtgaac gctctc //