LOCUS BBa_K1022126 4900 bp DNA linear 15-Jun-2024 DEFINITION pT7: RBS: His6- SUMO: Magainin II: RBS: TetR:TT : pTet: cI: TT : pcI: Ulp : Lysis ACCESSION BBa_K1022126 VERSION BBa_K1022126.1 FEATURES Location/Qualifiers promoter 1..28 /label=T7 RBS 29..42 /label=34 misc_feature 43..363 /label=His6:SUMO misc_feature 364..436 /label=Magainin II misc_feature 1..436 /label=BBa_K1022103 misc_feature 449..452 /label=conserved RBS 445..456 /label=BBa_B0034 CDS 466..1082 /label=tetR misc_feature 1083..1116 /label=SsrA misc_feature 1123..1147 /label=Help:Barcodes CDS 463..1122 /label=BBa_C0040 stem_loop 1167..1210 /label=stem_loop misc_feature 1156..1235 /label=BBa_B0010 terminator 1156..1235 /label=BBa_B0010 stem_loop 1251..1270 /label=T7 TE misc_feature 1277..1277 /label=stop polyA_site 1271..1284 /label=polya misc_feature 1244..1284 /label=BBa_B0012 terminator 1244..1284 /label=BBa_B0012 misc_feature 445..1284 /label=BBa_P0440 misc_feature 1..1284 /label=BBa_K1022106 misc_feature 1293..1311 /label=TetR 1 misc_feature 1293..1346 /label=BBa_R0040 promoter 1312..1317 /label=-35 misc_feature 1318..1336 /label=TetR 2 promoter 1335..1340 /label=-10 promoter 1293..1346 /label=BBa_R0040 misc_feature 1359..1362 /label=conserved RBS 1355..1366 /label=BBa_B0034 promoter 1293..1366 /label=BBa_J13002 CDS 1376..2083 /label=cI lambda misc_feature 2084..2116 /label=LVA misc_feature 2123..2147 /label=Help:Barcodes CDS 1373..2147 /label=BBa_C0051 stem_loop 2167..2210 /label=stem_loop misc_feature 2156..2235 /label=BBa_B0010 terminator 2156..2235 /label=BBa_B0010 stem_loop 2251..2270 /label=T7 TE misc_feature 2277..2277 /label=stop polyA_site 2271..2284 /label=polya misc_feature 2244..2284 /label=BBa_B0012 terminator 2244..2284 /label=BBa_B0012 terminator 2156..2284 /label=BBa_B0015 misc_feature 1293..2284 /label=BBa_K1022108 promoter 2293..2341 /label=cI RBS 2350..2366 /label=34 misc_feature 2367..3107 /label=Ulp misc_feature 2293..3107 /label=BBa_K1022107 misc_feature 1293..3107 /label=BBa_K1022115 misc_feature 3116..4900 /label=BBa_K1022109 misc_feature 1293..4900 /label=BBa_K1022123 ORIGIN 1 taatacgact cactataggg agagaaagag gagaaatact agatggctag catgcatcat 61 catcatcatc attcggactc agaagtcaat caagaagcta agccagaggt caagccagaa 121 gtcaagcctg agactcacat caatttaaag gtgtccgatg gatcttcaga gatcttcttc 181 aagatcaaaa agaccactcc tttaagaagg ctgatggaag cgttcgctaa acgtcagggt 241 aaggaaatgg actccttaag attcttgtac gacggtatta ggattcaagc tgatcagacc 301 cctgaagatt tggacatgga tgataacgat attattgagg ctcacagaga acagaccggt 361 ggtggtatcg gtaaattcct gcactctgcg aaaaaattcg gtaaagcgtt cgttggtcag 421 atcatgaact cttaattact agagaaagag gagaaatact agatgtccag attagataaa 481 agtaaagtga ttaacagcgc attagagctg cttaatgagg tcggaatcga aggtttaaca 541 acccgtaaac tcgcccagaa gctaggtgta gagcagccta cattgtattg gcatgtaaaa 601 aataagcggg ctttgctcga cgccttagcc attgagatgt tagataggca ccatactcac 661 ttttgccctt tagaagggga aagctggcaa gattttttac gtaataacgc taaaagtttt 721 agatgtgctt tactaagtca tcgcgatgga gcaaaagtac atttaggtac acggcctaca 781 gaaaaacagt atgaaactct cgaaaatcaa ttagcctttt tatgccaaca aggtttttca 841 ctagagaatg cattatatgc actcagcgct gtggggcatt ttactttagg ttgcgtattg 901 gaagatcaag agcatcaagt cgctaaagaa gaaagggaaa cacctactac tgatagtatg 961 ccgccattat tacgacaagc tatcgaatta tttgatcacc aaggtgcaga gccagccttc 1021 ttattcggcc ttgaattgat catatgcgga ttagaaaaac aacttaaatg tgaaagtggg 1081 tccgctgcaa acgacgaaaa ctacgcttta gtagcttaat aacactgata gtgctagtgt 1141 agatcactac tagagccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct 1201 ttcgttttat ctgttgtttg tcggtgaacg ctctctacta gagtcacact ggctcacctt 1261 cgggtgggcc tttctgcgtt tatatactag agtccctatc agtgatagag attgacatcc 1321 ctatcagtga tagagatact gagcactact agagaaagag gagaaatact agatgagcac 1381 aaaaaagaaa ccattaacac aagagcagct tgaggacgca cgtcgcctta aagcaattta 1441 tgaaaaaaag aaaaatgaac ttggcttatc ccaggaatct gtcgcagaca agatggggat 1501 ggggcagtca ggcgttggtg ctttatttaa tggcatcaat gcattaaatg cttataacgc 1561 cgcattgctt gcaaaaattc tcaaagttag cgttgaagaa tttagccctt caatcgccag 1621 agaaatctac gagatgtatg aagcggttag tatgcagccg tcacttagaa gtgagtatga 1681 gtaccctgtt ttttctcatg ttcaggcagg gatgttctca cctgagctta gaacctttac 1741 caaaggtgat gcggagagat gggtaagcac aaccaaaaaa gccagtgatt ctgcattctg 1801 gcttgaggtt gaaggtaatt ccatgaccgc accaacaggc tccaagccaa gctttcctga 1861 cggaatgtta attctcgttg accctgagca ggctgttgag ccaggtgatt tctgcatagc 1921 cagacttggg ggtgatgagt ttaccttcaa gaaactgatc agggatagcg gtcaggtgtt 1981 tttacaacca ctaaacccac agtacccaat gatcccatgc aatgagagtt gttccgttgt 2041 ggggaaagtt atcgctagtc agtggcctga agagacgttt ggcgctgcaa acgacgaaaa 2101 ctacgcttta gtagcttaat aacgctgata gtgctagtgt agatcgctac tagagccagg 2161 catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg 2221 tcggtgaacg ctctctacta gagtcacact ggctcacctt cgggtgggcc tttctgcgtt 2281 tatatactag agtaacaccg tgcgtgttga ctattttacc tctggcggtg ataatggttg 2341 cttaccggaa agaggagaaa tactagatgg ctagccttgt tcctgaatta aatgaaaaag 2401 acgatgacca agtacaaaaa gctttggcat cgagagaaaa tactcagtta atgaatagag 2461 ataatataga gataacagta cgtgatttta agaccttggc accacgaaga tggctaaatg 2521 acactatcat tgagtttttt atgaaataca ttgaaaaatc tacccctaat acagtggcgt 2581 ttaattcatt tttctatacc aatttatcag aaaggggtta tcaaggcgtc cggaggtgga 2641 tgaagagaaa gaagacacaa attgataaac ttgataaaat ctttacacca ataaatttga 2701 accaatccca ctgggcgttg ggcataattg atttaaaaaa gaaaactata ggttacgtag 2761 attcattatc gaatggtcca aatgctatga gtttcgctat actgactgac ttgcaaaaat 2821 atgttatgga ggaaagtaag catacaatag gagaagactt tgatttgatt catttagatt 2881 gtccgcagca accaaatggc tacgactgtg gaatatatgt ttgtatgaat actctctatg 2941 gaagtgcaga tgcgccattg gattttgatt ataaagatgc gattaggatg agaagattta 3001 ttgcccattt gattttaacc gacgctttaa aacgggatcc gaactcgagc tccgtcgaca 3061 agcttgcggc cgcactcgag caccaccacc accaccactg agtacattac tagagcttaa 3121 aaggagggtc tatggcagca cctagaatat cattttcgcc ctctgatatt ctatttggtg 3181 ttctcgatcg cttgttcaaa gataacgcta ccgggaaggt tcttgcttcc cgggtagctg 3241 tcgtaattct tttgtttata atggcgattg tttggtatag gggagatagt ttctttgagt 3301 actataagca atcaaagtat gaaacataca gtgaaattat tgaaaaggaa agaactgcac 3361 gctttgaatc tgtcgccctg gaacaactcc agatagttca tatatcatct gaggcagact 3421 ttagtgcggt gtattctttc cgccctaaaa acttaaacta ttttgttgat attatagcat 3481 acgaaggaaa attaccttca acaataagtg aaaaatcact tggaggatat cctgttgata 3541 aaactatgga tgaatataca gttcatttaa atggacgtca ttattattcc aactcaaaat 3601 ttgctttttt accaactaaa aagcctactc ccgaaataaa ctacatgtac agttgtccat 3661 attttaattt ggataatatc tatgctggaa cgataaccat gtactggtat agaaatgatc 3721 atataagtaa tgaccgcctt gaatcaatat gtgctcaggc ggccagaata ttaggaaggg 3781 ctaaataatt atactagaga tacttaggag gtattatgaa tatatttgaa atgttacgta 3841 tagatgaagg tcttagactt aaaatctata aagacacaga aggctattac actattggca 3901 tcggtcattt gcttacaaaa agtccatcac ttaatgctgc taaatctgaa ttagataaag 3961 ctattgggcg taattgcaat ggtgtaatta caaaagatga ggctgaaaaa ctctttaatc 4021 aggatgttga tgctgctgtt cgcggaatcc tgagaaatgc taaattaaaa ccggtttatg 4081 attctcttga tgcggttcgt cgctgtgcat tgattaatat ggttttccaa atgggagaaa 4141 ccggtgtggc aggatttact aactctttac gtatgcttca acaaaaacgc tgggatgaag 4201 cagcagttaa cttagctaaa agtagatggt ataatcaaac acctaatcgc gcaaaacgag 4261 tcattacaac gtttagaact ggcacttggg acgcgtataa aaatctataa agctactaga 4321 gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt 4381 tgtttgtcgg tgaacgctct ctactagagt cacactggct caccttcggg tgggcctttc 4441 tgcgtttata tactagagtt gacagctagc tcagtcctag ggactatgct agctactaga 4501 ggataggagg cctttatggc cttaaaagca acagcacttt ttgccatgct aggattgtca 4561 tttgttttat ctccatcgat tgaagcgaat gtcgatcctc attttgataa atttatggaa 4621 tctggtatta ggcacgttta tatgcttttt gaaaataaaa gcgtagaatc gtctgaacaa 4681 ttctatagtt ttatgagaac gacctataaa aatgacccgt gctcttctga ttttgaatgt 4741 atagagcgag gcgcggagat ggcacaatca tacgctagaa ttatgaacat taaattggag 4801 actgaatgaa attactagag ccaggcatca aataaaacga aaggctcagt cgaaagactg 4861 ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc //