##gff-version 3
##sequence-region BBa_K1022127 1 7154
BBa_K1022127	.	engineered_region	1	3538	.	+	0	ID=BBa_K1022120;Name=BBa_K1022120
BBa_K1022127	.	stop_codon	2188	2188	.	+	0	ID=annotation2356428;Name=stop;Parent=BBa_K1022120
BBa_K1022127	.	CDS	1429	2058	.	+	0	ID=annotation2356422;Name=agrA coding sequence;Parent=BBa_K1022120
BBa_K1022127	.	ribosome_entry_site	2703	2706	.	+	0	ID=annotation2356433;Name=34;Parent=BBa_K1022120
BBa_K1022127	.	engineered_region	1	130	.	+	0	ID=annotation2356412;Name=BBa_K206000;Parent=BBa_K1022120
BBa_K1022127	.	stem_loop	2078	2121	.	+	0	ID=annotation2356424;Name=stem_loop;Parent=BBa_K1022120
BBa_K1022127	.	sequence_feature	2635	2698	.	+	0	ID=annotation2356432;Name=Maximin H5;Parent=BBa_K1022120
BBa_K1022127	.	operator	61	78	.	+	0	ID=annotation2356415;Name=AraI2;Parent=BBa_K1022120
BBa_K1022127	.	ribosome_entry_site	1411	1422	.	+	0	ID=annotation2356421;Name=B0034-sequence RBS;Parent=BBa_K1022120
BBa_K1022127	.	engineered_region	2204	2299	.	+	0	ID=annotation2356429;Name=BBa_I746104;Parent=BBa_K1022120
BBa_K1022127	.	engineered_region	2155	2195	.	+	0	ID=annotation2356425;Name=BBa_B0012;Parent=BBa_K1022120
BBa_K1022127	.	operator	40	57	.	+	0	ID=annotation2356414;Name=AraI1;Parent=BBa_K1022120
BBa_K1022127	.	polyA_site	2182	2195	.	+	0	ID=annotation2356427;Name=polya;Parent=BBa_K1022120
BBa_K1022127	.	promoter	1	131	.	+	0	ID=annotation2356413;Name=promoter;Parent=BBa_K1022120
BBa_K1022127	.	CDS	2720	3335	.	+	0	ID=annotation2356434;Name=TetR;Parent=BBa_K1022120
BBa_K1022127	.	engineered_region	2067	2146	.	+	0	ID=annotation2356423;Name=BBa_B0010;Parent=BBa_K1022120
BBa_K1022127	.	tag	2314	2634	.	+	0	ID=annotation2356431;Name=HIs 6 - SUMO;Parent=BBa_K1022120
BBa_K1022127	.	engineered_region	139	2058	.	+	0	ID=annotation2356416;Name=BBa_I746100;Parent=BBa_K1022120
BBa_K1022127	.	engineered_region	1	3538	.	+	0	ID=annotation2356437;Name=BBa_K1022120;Parent=BBa_K1022120
BBa_K1022127	.	stop_codon	3410	3489	.	+	0	ID=annotation2356435;Name=B0010;Parent=BBa_K1022120
BBa_K1022127	.	sequence_feature	1405	1410	.	+	0	ID=annotation2356420;Name=BamHI site;Parent=BBa_K1022120
BBa_K1022127	.	ribosome_entry_site	139	150	.	+	0	ID=annotation2356417;Name=B0034-sequence RBS;Parent=BBa_K1022120
BBa_K1022127	.	CDS	157	1404	.	+	0	ID=annotation2356419;Name=agrC coding sequence;Parent=BBa_K1022120
BBa_K1022127	.	ribosome_entry_site	2300	2313	.	+	0	ID=annotation2356430;Name=34;Parent=BBa_K1022120
BBa_K1022127	.	stop_codon	3498	3538	.	+	0	ID=annotation2356436;Name=B0012;Parent=BBa_K1022120
BBa_K1022127	.	stem_loop	2162	2181	.	+	0	ID=annotation2356426;Name=T7 TE;Parent=BBa_K1022120
BBa_K1022127	.	engineered_region	1	2299	.	+	0	ID=annotation2356411;Name=BBa_K1022119;Parent=BBa_K1022120
BBa_K1022127	.	engineered_region	139	2299	.	+	0	ID=annotation2356418;Name=BBa_I746103;Parent=BBa_K1022120
BBa_K1022127	.	engineered_region	3547	7154	.	+	0	ID=BBa_K1022123;Name=BBa_K1022123
BBa_K1022127	.	engineered_region	5370	7154	.	+	0	ID=BBa_K1022109;Name=BBa_K1022109;Parent=BBa_K1022123
BBa_K1022127	.	engineered_region	3547	5361	.	+	0	ID=BBa_K1022115;Name=BBa_K1022115;Parent=BBa_K1022123
BBa_K1022127	.	sequence_feature	4547	5361	.	+	0	ID=BBa_K1022107;Name=BBa_K1022107;Parent=BBa_K1022115
BBa_K1022127	.	promoter	4547	4595	.	+	0	ID=annotation2335074;Name=cI;Parent=BBa_K1022107
BBa_K1022127	.	sequence_feature	4621	5361	.	+	0	ID=annotation2335076;Name=Ulp;Parent=BBa_K1022107
BBa_K1022127	.	ribosome_entry_site	4604	4620	.	+	0	ID=annotation2335075;Name=34;Parent=BBa_K1022107
BBa_K1022127	.	engineered_region	3547	4538	.	+	0	ID=BBa_K1022108;Name=BBa_K1022108;Parent=BBa_K1022115
BBa_K1022127	.	terminator	4410	4538	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K1022108
BBa_K1022127	.	terminator	4410	4489	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K1022127	.	stem_loop	4421	4464	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1022127	.	engineered_region	4410	4489	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1022127	.	terminator	4498	4538	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K1022127	.	engineered_region	4498	4538	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1022127	.	stop_codon	4531	4531	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1022127	.	polyA_site	4525	4538	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1022127	.	stem_loop	4505	4524	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1022127	.	CDS	3627	4401	.	+	0	ID=BBa_C0051;Name=BBa_C0051;Parent=BBa_K1022108
BBa_K1022127	.	tag	4338	4370	.	+	0	ID=annotation23335;Name=LVA;Parent=BBa_C0051
BBa_K1022127	.	engineered_tag	4377	4401	.	+	0	ID=annotation2213991;Name=Help:Barcodes;Parent=BBa_C0051
BBa_K1022127	.	CDS	3630	4337	.	+	0	ID=annotation23334;Name=cI lambda;Parent=BBa_C0051
BBa_K1022127	.	promoter	3547	3620	.	+	0	ID=BBa_J13002;Name=BBa_J13002;Parent=BBa_K1022108
BBa_K1022127	.	promoter	3547	3600	.	+	0	ID=BBa_R0040;Name=BBa_R0040;Parent=BBa_J13002
BBa_K1022127	.	promoter	3566	3571	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_K1022127	.	non_covalent_binding_site	3572	3590	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_K1022127	.	engineered_region	3547	3600	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_K1022127	.	non_covalent_binding_site	3547	3565	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_K1022127	.	promoter	3589	3594	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_K1022127	.	ribosome_entry_site	3609	3620	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_J13002
BBa_K1022127	.	conserved_region	3613	3616	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1022127	.	terminator	2155	2195	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_K1022127	.	engineered_region	2155	2195	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1022127	.	stop_codon	2188	2188	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1022127	.	polyA_site	2182	2195	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1022127	.	stem_loop	2162	2181	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1022127	.	terminator	2067	2146	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_K1022127	.	stem_loop	2078	2121	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1022127	.	engineered_region	2067	2146	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
>BBa_K1022127
acattgattatttgcacggcgtcacactttgctatgccatagcaagatagtccataagattagcggatcctacctgacg
ctttttatcgcaactctctactgtttctccataccgtttttttgggctagctactagagaaagaggagaaatactagat
gattctgatgttcaccattccagcaattattagcggtattaagtacagcaagctggattatttcttcatcatcgtaatc
tctaccctgtccctgttcctgttcaaaatgttcgattccgcgagcctgattatcctgacttccttcatcatcatcatgt
atttcgtaaaaatcaaatggtatagcatcctgctgatcatgacctcccagattatcctgtactgtgccaactacatgta
tatcgttatctacgcgtacatcacgaaaatctccgattctatcttcgttatctttccgtctttcttcgtggtgtacgtt
acgatctctattctgttttcctatattatcaatcgcgttctgaaaaagatctctactccgtacctgattctgaacaaag
gttttctgatcgtcatctccactatcctgctgctgaccttctctctgttcttcttttactctcagatcaactccgatga
ggcaaaagtcatccgtcagtacagcttcatctttatcggtattactatcttcctgagcattctgactttcgttatcagc
cagttcctgctgaaagaaatgaagtacaagcgcaaccaagaagaaatcgagacttactacgagtacaccctgaagattg
aagcgatcaacaacgaaatgcgtaaatttcgtcacgactacgttaacattctgaccaccctgagcgaatacatccgtga
agacgacatgcctggcctgcgtgattatttcaacaaaaacattgtgccgatgaaagataacctgcaaatgaacgctatt
aagctgaacggtatcgaaaacctgaaagtacgtgagatcaagggtctgattaccgctaaaatcctgcgtgcgcaggaaa
tgaacatcccaattagcatcgaaatcccagacgaagtttctagcattaacctgaacatgattgacctgtctcgtagcat
cggcatcatcctggacaacgctatcgaagcttccactgaaatcgacgacccgattattcgcgttgcttttatcgagagc
gaaaactccgtgacctttatcgtgatgaacaaatgcgcggacgatatcccgcgtattcacgaactgttccaggaatctt
tcagcaccaagggtgaaggtcgcggcctgggcctgtccaccctgaaagagatcgcggataacgccgacaacgttctgct
ggacactattatcgaaaacggtttcttcattcagaaagtcgaaatcatcaataactaataaggatccaaagaggagaaa
tactagatggaaatcgcactggctacggacaatccatatgaggttctggaacaggctaaaaacatgaacgacatcggct
gctatttcctggatattcagctgagcaccgacatcaacggtatcaaactgggctctgaaatccgtaaacatgatccggt
tggtaacattattttcgtgacttctcattctgagctgacctatctgacgttcgtatacaaggttgcagccatggatttc
atctttaaagacgatccggcagagctgcgtacgcgcatcatcgactgtctggaaactgcgcacacgcgcctgcaactgc
tgtccaaagataactccgtggaaaccattgaactgaaacgtggcagcaacagcgtttacgttcagtacgatgatatcat
gttcttcgaaagctccactaagtcccaccgtctgatcgcgcatctggataatcgtcagatcgagttctatggcaacctg
aaagagctgagccagctggacgatcgcttcttccgttgtcataactccttcgttgttaaccgtcacaacatcgaatcca
tcgactccaaagagcgtattgtatacttcaagaacaaagaacactgttatgcgtctgtacgcaatgtcaagaaaatcta
ataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtc
ggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagattaaatac
aaattacatttaacagttaagtatttatttcctacagttaggcaatataatgataaaagattgtactaaatcgtataat
gacagtgaaggagaaatactagatggctagcatgcatcatcatcatcatcattcggactcagaagtcaatcaagaagct
aagccagaggtcaagccagaagtcaagcctgagactcacatcaatttaaaggtgtccgatggatcttcagagatcttct
tcaagatcaaaaagaccactcctttaagaaggctgatggaagcgttcgctaaacgtcagggtaaggaaatggactcctt
aagattcttgtacgacggtattaggattcaagctgatcagacccctgaagatttggacatggatgataacgatattatt
gaggctcacagagaacagaccggtggtatcctgggtccggttctgggtctggtttctgacaccctggacgacgttctgg
gtatcctgtaataaagaggagaaatactagatgtccagattagataaaagtaaagtgattaacagcgcattagagctgc
ttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattg
gcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccct
ttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatg
gagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgcca
acaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagat
caagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcg
aattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaact
taaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtaga
tcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtc
ggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtccctatca
gtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatactagatgagcac
aaaaaagaaaccattaacacaagagcagcttgaggacgcacgtcgccttaaagcaatttatgaaaaaaagaaaaatgaa
cttggcttatcccaggaatctgtcgcagacaagatggggatggggcagtcaggcgttggtgctttatttaatggcatca
atgcattaaatgcttataacgccgcattgcttgcaaaaattctcaaagttagcgttgaagaatttagcccttcaatcgc
cagagaaatctacgagatgtatgaagcggttagtatgcagccgtcacttagaagtgagtatgagtaccctgttttttct
catgttcaggcagggatgttctcacctgagcttagaacctttaccaaaggtgatgcggagagatgggtaagcacaacca
aaaaagccagtgattctgcattctggcttgaggttgaaggtaattccatgaccgcaccaacaggctccaagccaagctt
tcctgacggaatgttaattctcgttgaccctgagcaggctgttgagccaggtgatttctgcatagccagacttgggggt
gatgagtttaccttcaagaaactgatcagggatagcggtcaggtgtttttacaaccactaaacccacagtacccaatga
tcccatgcaatgagagttgttccgttgtggggaaagttatcgctagtcagtggcctgaagagacgtttggcgctgcaaa
cgacgaaaactacgctttagtagcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataa
aacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacac
tggctcaccttcgggtgggcctttctgcgtttatatactagagtaacaccgtgcgtgttgactattttacctctggcgg
tgataatggttgcttaccggaaagaggagaaatactagatggctagccttgttcctgaattaaatgaaaaagacgatga
ccaagtacaaaaagctttggcatcgagagaaaatactcagttaatgaatagagataatatagagataacagtacgtgat
tttaagaccttggcaccacgaagatggctaaatgacactatcattgagttttttatgaaatacattgaaaaatctaccc
ctaatacagtggcgtttaattcatttttctataccaatttatcagaaaggggttatcaaggcgtccggaggtggatgaa
gagaaagaagacacaaattgataaacttgataaaatctttacaccaataaatttgaaccaatcccactgggcgttgggc
ataattgatttaaaaaagaaaactataggttacgtagattcattatcgaatggtccaaatgctatgagtttcgctatac
tgactgacttgcaaaaatatgttatggaggaaagtaagcatacaataggagaagactttgatttgattcatttagattg
tccgcagcaaccaaatggctacgactgtggaatatatgtttgtatgaatactctctatggaagtgcagatgcgccattg
gattttgattataaagatgcgattaggatgagaagatttattgcccatttgattttaaccgacgctttaaaacgggatc
cgaactcgagctccgtcgacaagcttgcggccgcactcgagcaccaccaccaccaccactgagtacattactagagctt
aaaaggagggtctatggcagcacctagaatatcattttcgccctctgatattctatttggtgttctcgatcgcttgttc
aaagataacgctaccgggaaggttcttgcttcccgggtagctgtcgtaattcttttgtttataatggcgattgtttggt
ataggggagatagtttctttgagtactataagcaatcaaagtatgaaacatacagtgaaattattgaaaaggaaagaac
tgcacgctttgaatctgtcgccctggaacaactccagatagttcatatatcatctgaggcagactttagtgcggtgtat
tctttccgccctaaaaacttaaactattttgttgatattatagcatacgaaggaaaattaccttcaacaataagtgaaa
aatcacttggaggatatcctgttgataaaactatggatgaatatacagttcatttaaatggacgtcattattattccaa
ctcaaaatttgcttttttaccaactaaaaagcctactcccgaaataaactacatgtacagttgtccatattttaatttg
gataatatctatgctggaacgataaccatgtactggtatagaaatgatcatataagtaatgaccgccttgaatcaatat
gtgctcaggcggccagaatattaggaagggctaaataattatactagagatacttaggaggtattatgaatatatttga
aatgttacgtatagatgaaggtcttagacttaaaatctataaagacacagaaggctattacactattggcatcggtcat
ttgcttacaaaaagtccatcacttaatgctgctaaatctgaattagataaagctattgggcgtaattgcaatggtgtaa
ttacaaaagatgaggctgaaaaactctttaatcaggatgttgatgctgctgttcgcggaatcctgagaaatgctaaatt
aaaaccggtttatgattctcttgatgcggttcgtcgctgtgcattgattaatatggttttccaaatgggagaaaccggt
gtggcaggatttactaactctttacgtatgcttcaacaaaaacgctgggatgaagcagcagttaacttagctaaaagta
gatggtataatcaaacacctaatcgcgcaaaacgagtcattacaacgtttagaactggcacttgggacgcgtataaaaa
tctataaagctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttg
tttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagttg
acagctagctcagtcctagggactatgctagctactagaggataggaggcctttatggccttaaaagcaacagcacttt
ttgccatgctaggattgtcatttgttttatctccatcgattgaagcgaatgtcgatcctcattttgataaatttatgga
atctggtattaggcacgtttatatgctttttgaaaataaaagcgtagaatcgtctgaacaattctatagttttatgaga
acgacctataaaaatgacccgtgctcttctgattttgaatgtatagagcgaggcgcggagatggcacaatcatacgcta
gaattatgaacattaaattggagactgaatgaaattactagagccaggcatcaaataaaacgaaaggctcagtcgaaag
actgggcctttcgttttatctgttgtttgtcggtgaacgctctc