LOCUS BBa_K1022128 7139 bp DNA linear 05-Nov-2024 DEFINITION pBAD: Agr A : Agr C : pP2 : RBS: His6- SUMO: Signiferin: RBS TetR :TT : pTet: cI: TT : pcI: Ulp : Ly ACCESSION BBa_K1022128 VERSION BBa_K1022128.1 FEATURES Location/Qualifiers stem_loop 2078..2121 /label=stem_loop misc_feature 2067..2146 /label=BBa_B0010 terminator 2067..2146 /label=BBa_B0010 stem_loop 2162..2181 /label=T7 TE misc_feature 2188..2188 /label=stop polyA_site 2182..2195 /label=polya misc_feature 2155..2195 /label=BBa_B0012 terminator 2155..2195 /label=BBa_B0012 promoter 1..131 /label=promoter misc_feature 1..130 /label=BBa_K206000 misc_feature 40..57 /label=AraI1 misc_feature 61..78 /label=AraI2 misc_feature 139..2299 /label=BBa_I746103 misc_feature 139..2058 /label=BBa_I746100 RBS 139..150 /label=B0034-sequence RBS CDS 157..1404 /label=agrC coding sequence misc_feature 1405..1410 /label=BamHI site RBS 1411..1422 /label=B0034-sequence RBS CDS 1429..2058 /label=agrA coding sequence misc_feature 2067..2146 /label=BBa_B0010 stem_loop 2078..2121 /label=stem_loop misc_feature 2155..2195 /label=BBa_B0012 stem_loop 2162..2181 /label=T7 TE polyA_site 2182..2195 /label=polya misc_feature 2188..2188 /label=stop misc_feature 2204..2299 /label=BBa_I746104 misc_feature 1..2299 /label=BBa_K1022119 RBS 2300..2313 /label=34 misc_feature 2314..2634 /label=His 6 SUMO misc_feature 2635..2683 /label=Signiferin RBS 2688..2691 /label=34 CDS 2705..3320 /label=Tet R misc_feature 3395..3474 /label=B0010 misc_feature 3483..3523 /label=B0012 misc_feature 1..3523 /label=BBa_K1022121 misc_feature 3532..3550 /label=TetR 1 misc_feature 3532..3585 /label=BBa_R0040 promoter 3551..3556 /label=-35 misc_feature 3557..3575 /label=TetR 2 promoter 3574..3579 /label=-10 promoter 3532..3585 /label=BBa_R0040 misc_feature 3598..3601 /label=conserved RBS 3594..3605 /label=BBa_B0034 promoter 3532..3605 /label=BBa_J13002 CDS 3615..4322 /label=cI lambda misc_feature 4323..4355 /label=LVA misc_feature 4362..4386 /label=Help:Barcodes CDS 3612..4386 /label=BBa_C0051 stem_loop 4406..4449 /label=stem_loop misc_feature 4395..4474 /label=BBa_B0010 terminator 4395..4474 /label=BBa_B0010 stem_loop 4490..4509 /label=T7 TE misc_feature 4516..4516 /label=stop polyA_site 4510..4523 /label=polya misc_feature 4483..4523 /label=BBa_B0012 terminator 4483..4523 /label=BBa_B0012 terminator 4395..4523 /label=BBa_B0015 misc_feature 3532..4523 /label=BBa_K1022108 promoter 4532..4580 /label=cI RBS 4589..4605 /label=34 misc_feature 4606..5346 /label=Ulp misc_feature 4532..5346 /label=BBa_K1022107 misc_feature 3532..5346 /label=BBa_K1022115 misc_feature 5355..7139 /label=BBa_K1022109 misc_feature 3532..7139 /label=BBa_K1022123 ORIGIN 1 acattgatta tttgcacggc gtcacacttt gctatgccat agcaagatag tccataagat 61 tagcggatcc tacctgacgc tttttatcgc aactctctac tgtttctcca taccgttttt 121 ttgggctagc tactagagaa agaggagaaa tactagatga ttctgatgtt caccattcca 181 gcaattatta gcggtattaa gtacagcaag ctggattatt tcttcatcat cgtaatctct 241 accctgtccc tgttcctgtt caaaatgttc gattccgcga gcctgattat cctgacttcc 301 ttcatcatca tcatgtattt cgtaaaaatc aaatggtata gcatcctgct gatcatgacc 361 tcccagatta tcctgtactg tgccaactac atgtatatcg ttatctacgc gtacatcacg 421 aaaatctccg attctatctt cgttatcttt ccgtctttct tcgtggtgta cgttacgatc 481 tctattctgt tttcctatat tatcaatcgc gttctgaaaa agatctctac tccgtacctg 541 attctgaaca aaggttttct gatcgtcatc tccactatcc tgctgctgac cttctctctg 601 ttcttctttt actctcagat caactccgat gaggcaaaag tcatccgtca gtacagcttc 661 atctttatcg gtattactat cttcctgagc attctgactt tcgttatcag ccagttcctg 721 ctgaaagaaa tgaagtacaa gcgcaaccaa gaagaaatcg agacttacta cgagtacacc 781 ctgaagattg aagcgatcaa caacgaaatg cgtaaatttc gtcacgacta cgttaacatt 841 ctgaccaccc tgagcgaata catccgtgaa gacgacatgc ctggcctgcg tgattatttc 901 aacaaaaaca ttgtgccgat gaaagataac ctgcaaatga acgctattaa gctgaacggt 961 atcgaaaacc tgaaagtacg tgagatcaag ggtctgatta ccgctaaaat cctgcgtgcg 1021 caggaaatga acatcccaat tagcatcgaa atcccagacg aagtttctag cattaacctg 1081 aacatgattg acctgtctcg tagcatcggc atcatcctgg acaacgctat cgaagcttcc 1141 actgaaatcg acgacccgat tattcgcgtt gcttttatcg agagcgaaaa ctccgtgacc 1201 tttatcgtga tgaacaaatg cgcggacgat atcccgcgta ttcacgaact gttccaggaa 1261 tctttcagca ccaagggtga aggtcgcggc ctgggcctgt ccaccctgaa agagatcgcg 1321 gataacgccg acaacgttct gctggacact attatcgaaa acggtttctt cattcagaaa 1381 gtcgaaatca tcaataacta ataaggatcc aaagaggaga aatactagat ggaaatcgca 1441 ctggctacgg acaatccata tgaggttctg gaacaggcta aaaacatgaa cgacatcggc 1501 tgctatttcc tggatattca gctgagcacc gacatcaacg gtatcaaact gggctctgaa 1561 atccgtaaac atgatccggt tggtaacatt attttcgtga cttctcattc tgagctgacc 1621 tatctgacgt tcgtatacaa ggttgcagcc atggatttca tctttaaaga cgatccggca 1681 gagctgcgta cgcgcatcat cgactgtctg gaaactgcgc acacgcgcct gcaactgctg 1741 tccaaagata actccgtgga aaccattgaa ctgaaacgtg gcagcaacag cgtttacgtt 1801 cagtacgatg atatcatgtt cttcgaaagc tccactaagt cccaccgtct gatcgcgcat 1861 ctggataatc gtcagatcga gttctatggc aacctgaaag agctgagcca gctggacgat 1921 cgcttcttcc gttgtcataa ctccttcgtt gttaaccgtc acaacatcga atccatcgac 1981 tccaaagagc gtattgtata cttcaagaac aaagaacact gttatgcgtc tgtacgcaat 2041 gtcaagaaaa tctaataata ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa 2101 agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctctact agagtcacac 2161 tggctcacct tcgggtgggc ctttctgcgt ttatatacta gagattaaat acaaattaca 2221 tttaacagtt aagtatttat ttcctacagt taggcaatat aatgataaaa gattgtacta 2281 aatcgtataa tgacagtgaa ggagaaatac tagatggcta gcatgcatca tcatcatcat 2341 cattcggact cagaagtcaa tcaagaagct aagccagagg tcaagccaga agtcaagcct 2401 gagactcaca tcaatttaaa ggtgtccgat ggatcttcag agatcttctt caagatcaaa 2461 aagaccactc ctttaagaag gctgatggaa gcgttcgcta aacgtcaggg taaggaaatg 2521 gactccttaa gattcttgta cgacggtatt aggattcaag ctgatcagac ccctgaagat 2581 ttggacatgg atgataacga tattattgag gctcacagag aacagaccgg tggtatcatc 2641 ggtcacctga tcaaaaccgc gctgggtatg ctgggtctgt aataaagagg agaaatacta 2701 gatgtccaga ttagataaaa gtaaagtgat taacagcgca ttagagctgc ttaatgaggt 2761 cggaatcgaa ggtttaacaa cccgtaaact cgcccagaag ctaggtgtag agcagcctac 2821 attgtattgg catgtaaaaa ataagcgggc tttgctcgac gccttagcca ttgagatgtt 2881 agataggcac catactcact tttgcccttt agaaggggaa agctggcaag attttttacg 2941 taataacgct aaaagtttta gatgtgcttt actaagtcat cgcgatggag caaaagtaca 3001 tttaggtaca cggcctacag aaaaacagta tgaaactctc gaaaatcaat tagccttttt 3061 atgccaacaa ggtttttcac tagagaatgc attatatgca ctcagcgctg tggggcattt 3121 tactttaggt tgcgtattgg aagatcaaga gcatcaagtc gctaaagaag aaagggaaac 3181 acctactact gatagtatgc cgccattatt acgacaagct atcgaattat ttgatcacca 3241 aggtgcagag ccagccttct tattcggcct tgaattgatc atatgcggat tagaaaaaca 3301 acttaaatgt gaaagtgggt ccgctgcaaa cgacgaaaac tacgctttag tagcttaata 3361 acactgatag tgctagtgta gatcactact agagccaggc atcaaataaa acgaaaggct 3421 cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctctactag 3481 agtcacactg gctcaccttc gggtgggcct ttctgcgttt atatactaga gtccctatca 3541 gtgatagaga ttgacatccc tatcagtgat agagatactg agcactacta gagaaagagg 3601 agaaatacta gatgagcaca aaaaagaaac cattaacaca agagcagctt gaggacgcac 3661 gtcgccttaa agcaatttat gaaaaaaaga aaaatgaact tggcttatcc caggaatctg 3721 tcgcagacaa gatggggatg gggcagtcag gcgttggtgc tttatttaat ggcatcaatg 3781 cattaaatgc ttataacgcc gcattgcttg caaaaattct caaagttagc gttgaagaat 3841 ttagcccttc aatcgccaga gaaatctacg agatgtatga agcggttagt atgcagccgt 3901 cacttagaag tgagtatgag taccctgttt tttctcatgt tcaggcaggg atgttctcac 3961 ctgagcttag aacctttacc aaaggtgatg cggagagatg ggtaagcaca accaaaaaag 4021 ccagtgattc tgcattctgg cttgaggttg aaggtaattc catgaccgca ccaacaggct 4081 ccaagccaag ctttcctgac ggaatgttaa ttctcgttga ccctgagcag gctgttgagc 4141 caggtgattt ctgcatagcc agacttgggg gtgatgagtt taccttcaag aaactgatca 4201 gggatagcgg tcaggtgttt ttacaaccac taaacccaca gtacccaatg atcccatgca 4261 atgagagttg ttccgttgtg gggaaagtta tcgctagtca gtggcctgaa gagacgtttg 4321 gcgctgcaaa cgacgaaaac tacgctttag tagcttaata acgctgatag tgctagtgta 4381 gatcgctact agagccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt 4441 tcgttttatc tgttgtttgt cggtgaacgc tctctactag agtcacactg gctcaccttc 4501 gggtgggcct ttctgcgttt atatactaga gtaacaccgt gcgtgttgac tattttacct 4561 ctggcggtga taatggttgc ttaccggaaa gaggagaaat actagatggc tagccttgtt 4621 cctgaattaa atgaaaaaga cgatgaccaa gtacaaaaag ctttggcatc gagagaaaat 4681 actcagttaa tgaatagaga taatatagag ataacagtac gtgattttaa gaccttggca 4741 ccacgaagat ggctaaatga cactatcatt gagtttttta tgaaatacat tgaaaaatct 4801 acccctaata cagtggcgtt taattcattt ttctatacca atttatcaga aaggggttat 4861 caaggcgtcc ggaggtggat gaagagaaag aagacacaaa ttgataaact tgataaaatc 4921 tttacaccaa taaatttgaa ccaatcccac tgggcgttgg gcataattga tttaaaaaag 4981 aaaactatag gttacgtaga ttcattatcg aatggtccaa atgctatgag tttcgctata 5041 ctgactgact tgcaaaaata tgttatggag gaaagtaagc atacaatagg agaagacttt 5101 gatttgattc atttagattg tccgcagcaa ccaaatggct acgactgtgg aatatatgtt 5161 tgtatgaata ctctctatgg aagtgcagat gcgccattgg attttgatta taaagatgcg 5221 attaggatga gaagatttat tgcccatttg attttaaccg acgctttaaa acgggatccg 5281 aactcgagct ccgtcgacaa gcttgcggcc gcactcgagc accaccacca ccaccactga 5341 gtacattact agagcttaaa aggagggtct atggcagcac ctagaatatc attttcgccc 5401 tctgatattc tatttggtgt tctcgatcgc ttgttcaaag ataacgctac cgggaaggtt 5461 cttgcttccc gggtagctgt cgtaattctt ttgtttataa tggcgattgt ttggtatagg 5521 ggagatagtt tctttgagta ctataagcaa tcaaagtatg aaacatacag tgaaattatt 5581 gaaaaggaaa gaactgcacg ctttgaatct gtcgccctgg aacaactcca gatagttcat 5641 atatcatctg aggcagactt tagtgcggtg tattctttcc gccctaaaaa cttaaactat 5701 tttgttgata ttatagcata cgaaggaaaa ttaccttcaa caataagtga aaaatcactt 5761 ggaggatatc ctgttgataa aactatggat gaatatacag ttcatttaaa tggacgtcat 5821 tattattcca actcaaaatt tgctttttta ccaactaaaa agcctactcc cgaaataaac 5881 tacatgtaca gttgtccata ttttaatttg gataatatct atgctggaac gataaccatg 5941 tactggtata gaaatgatca tataagtaat gaccgccttg aatcaatatg tgctcaggcg 6001 gccagaatat taggaagggc taaataatta tactagagat acttaggagg tattatgaat 6061 atatttgaaa tgttacgtat agatgaaggt cttagactta aaatctataa agacacagaa 6121 ggctattaca ctattggcat cggtcatttg cttacaaaaa gtccatcact taatgctgct 6181 aaatctgaat tagataaagc tattgggcgt aattgcaatg gtgtaattac aaaagatgag 6241 gctgaaaaac tctttaatca ggatgttgat gctgctgttc gcggaatcct gagaaatgct 6301 aaattaaaac cggtttatga ttctcttgat gcggttcgtc gctgtgcatt gattaatatg 6361 gttttccaaa tgggagaaac cggtgtggca ggatttacta actctttacg tatgcttcaa 6421 caaaaacgct gggatgaagc agcagttaac ttagctaaaa gtagatggta taatcaaaca 6481 cctaatcgcg caaaacgagt cattacaacg tttagaactg gcacttggga cgcgtataaa 6541 aatctataaa gctactagag ccaggcatca aataaaacga aaggctcagt cgaaagactg 6601 ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc tactagagtc acactggctc 6661 accttcgggt gggcctttct gcgtttatat actagagttg acagctagct cagtcctagg 6721 gactatgcta gctactagag gataggaggc ctttatggcc ttaaaagcaa cagcactttt 6781 tgccatgcta ggattgtcat ttgttttatc tccatcgatt gaagcgaatg tcgatcctca 6841 ttttgataaa tttatggaat ctggtattag gcacgtttat atgctttttg aaaataaaag 6901 cgtagaatcg tctgaacaat tctatagttt tatgagaacg acctataaaa atgacccgtg 6961 ctcttctgat tttgaatgta tagagcgagg cgcggagatg gcacaatcat acgctagaat 7021 tatgaacatt aaattggaga ctgaatgaaa ttactagagc caggcatcaa ataaaacgaa 7081 aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctc //