##gff-version 3
##sequence-region BBa_K1022128 1 7139
BBa_K1022128	.	engineered_region	1	3523	.	+	0	ID=BBa_K1022121;Name=BBa_K1022121
BBa_K1022128	.	operator	61	78	.	+	0	ID=annotation2356441;Name=AraI2;Parent=BBa_K1022121
BBa_K1022128	.	ribosome_entry_site	2300	2313	.	+	0	ID=annotation2356457;Name=34;Parent=BBa_K1022121
BBa_K1022128	.	stem_loop	2162	2181	.	+	0	ID=annotation2356452;Name=T7 TE;Parent=BBa_K1022121
BBa_K1022128	.	sequence_feature	1405	1410	.	+	0	ID=annotation2356446;Name=BamHI site;Parent=BBa_K1022121
BBa_K1022128	.	engineered_region	2067	2146	.	+	0	ID=annotation2356449;Name=BBa_B0010;Parent=BBa_K1022121
BBa_K1022128	.	stop_codon	3483	3523	.	+	0	ID=annotation2356521;Name=B0012;Parent=BBa_K1022121
BBa_K1022128	.	engineered_region	2155	2195	.	+	0	ID=annotation2356451;Name=BBa_B0012;Parent=BBa_K1022121
BBa_K1022128	.	engineered_region	139	2058	.	+	0	ID=annotation2356443;Name=BBa_I746100;Parent=BBa_K1022121
BBa_K1022128	.	promoter	1	131	.	+	0	ID=annotation2356438;Name=promoter;Parent=BBa_K1022121
BBa_K1022128	.	stop_codon	2188	2188	.	+	0	ID=annotation2356454;Name=stop;Parent=BBa_K1022121
BBa_K1022128	.	tag	2314	2634	.	+	0	ID=annotation2356458;Name=His 6 SUMO ;Parent=BBa_K1022121
BBa_K1022128	.	ribosome_entry_site	2688	2691	.	+	0	ID=annotation2356492;Name=34;Parent=BBa_K1022121
BBa_K1022128	.	engineered_region	139	2299	.	+	0	ID=annotation2356442;Name=BBa_I746103;Parent=BBa_K1022121
BBa_K1022128	.	engineered_region	1	130	.	+	0	ID=annotation2356439;Name=BBa_K206000;Parent=BBa_K1022121
BBa_K1022128	.	engineered_region	1	2299	.	+	0	ID=annotation2356456;Name=BBa_K1022119;Parent=BBa_K1022121
BBa_K1022128	.	stop_codon	3395	3474	.	+	0	ID=annotation2356506;Name=B0010;Parent=BBa_K1022121
BBa_K1022128	.	stem_loop	2078	2121	.	+	0	ID=annotation2356450;Name=stem_loop;Parent=BBa_K1022121
BBa_K1022128	.	polyA_site	2182	2195	.	+	0	ID=annotation2356453;Name=polya;Parent=BBa_K1022121
BBa_K1022128	.	operator	40	57	.	+	0	ID=annotation2356440;Name=AraI1;Parent=BBa_K1022121
BBa_K1022128	.	CDS	157	1404	.	+	0	ID=annotation2356445;Name=agrC coding sequence;Parent=BBa_K1022121
BBa_K1022128	.	engineered_region	2204	2299	.	+	0	ID=annotation2356455;Name=BBa_I746104;Parent=BBa_K1022121
BBa_K1022128	.	ribosome_entry_site	139	150	.	+	0	ID=annotation2356444;Name=B0034-sequence RBS;Parent=BBa_K1022121
BBa_K1022128	.	CDS	1429	2058	.	+	0	ID=annotation2356448;Name=agrA coding sequence;Parent=BBa_K1022121
BBa_K1022128	.	sequence_feature	2635	2683	.	+	0	ID=annotation2356483;Name=Signiferin ;Parent=BBa_K1022121
BBa_K1022128	.	ribosome_entry_site	1411	1422	.	+	0	ID=annotation2356447;Name=B0034-sequence RBS;Parent=BBa_K1022121
BBa_K1022128	.	CDS	2705	3320	.	+	0	ID=annotation2356493;Name=Tet R;Parent=BBa_K1022121
BBa_K1022128	.	terminator	2067	2146	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_K1022128	.	engineered_region	2067	2146	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1022128	.	stem_loop	2078	2121	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1022128	.	terminator	2155	2195	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_K1022128	.	stem_loop	2162	2181	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1022128	.	stop_codon	2188	2188	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1022128	.	engineered_region	2155	2195	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1022128	.	polyA_site	2182	2195	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1022128	.	engineered_region	3532	7139	.	+	0	ID=BBa_K1022123;Name=BBa_K1022123
BBa_K1022128	.	engineered_region	5355	7139	.	+	0	ID=BBa_K1022109;Name=BBa_K1022109;Parent=BBa_K1022123
BBa_K1022128	.	engineered_region	3532	5346	.	+	0	ID=BBa_K1022115;Name=BBa_K1022115;Parent=BBa_K1022123
BBa_K1022128	.	sequence_feature	4532	5346	.	+	0	ID=BBa_K1022107;Name=BBa_K1022107;Parent=BBa_K1022115
BBa_K1022128	.	ribosome_entry_site	4589	4605	.	+	0	ID=annotation2335075;Name=34;Parent=BBa_K1022107
BBa_K1022128	.	promoter	4532	4580	.	+	0	ID=annotation2335074;Name=cI;Parent=BBa_K1022107
BBa_K1022128	.	sequence_feature	4606	5346	.	+	0	ID=annotation2335076;Name=Ulp;Parent=BBa_K1022107
BBa_K1022128	.	engineered_region	3532	4523	.	+	0	ID=BBa_K1022108;Name=BBa_K1022108;Parent=BBa_K1022115
BBa_K1022128	.	terminator	4395	4523	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K1022108
BBa_K1022128	.	terminator	4395	4474	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K1022128	.	engineered_region	4395	4474	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1022128	.	stem_loop	4406	4449	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1022128	.	terminator	4483	4523	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K1022128	.	stem_loop	4490	4509	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1022128	.	stop_codon	4516	4516	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1022128	.	engineered_region	4483	4523	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1022128	.	polyA_site	4510	4523	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1022128	.	CDS	3612	4386	.	+	0	ID=BBa_C0051;Name=BBa_C0051;Parent=BBa_K1022108
BBa_K1022128	.	CDS	3615	4322	.	+	0	ID=annotation23334;Name=cI lambda;Parent=BBa_C0051
BBa_K1022128	.	engineered_tag	4362	4386	.	+	0	ID=annotation2213991;Name=Help:Barcodes;Parent=BBa_C0051
BBa_K1022128	.	tag	4323	4355	.	+	0	ID=annotation23335;Name=LVA;Parent=BBa_C0051
BBa_K1022128	.	promoter	3532	3605	.	+	0	ID=BBa_J13002;Name=BBa_J13002;Parent=BBa_K1022108
BBa_K1022128	.	promoter	3532	3585	.	+	0	ID=BBa_R0040;Name=BBa_R0040;Parent=BBa_J13002
BBa_K1022128	.	promoter	3574	3579	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_K1022128	.	promoter	3551	3556	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_K1022128	.	non_covalent_binding_site	3532	3550	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_K1022128	.	non_covalent_binding_site	3557	3575	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_K1022128	.	engineered_region	3532	3585	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_K1022128	.	ribosome_entry_site	3594	3605	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_J13002
BBa_K1022128	.	conserved_region	3598	3601	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
>BBa_K1022128
acattgattatttgcacggcgtcacactttgctatgccatagcaagatagtccataagattagcggatcctacctgacg
ctttttatcgcaactctctactgtttctccataccgtttttttgggctagctactagagaaagaggagaaatactagat
gattctgatgttcaccattccagcaattattagcggtattaagtacagcaagctggattatttcttcatcatcgtaatc
tctaccctgtccctgttcctgttcaaaatgttcgattccgcgagcctgattatcctgacttccttcatcatcatcatgt
atttcgtaaaaatcaaatggtatagcatcctgctgatcatgacctcccagattatcctgtactgtgccaactacatgta
tatcgttatctacgcgtacatcacgaaaatctccgattctatcttcgttatctttccgtctttcttcgtggtgtacgtt
acgatctctattctgttttcctatattatcaatcgcgttctgaaaaagatctctactccgtacctgattctgaacaaag
gttttctgatcgtcatctccactatcctgctgctgaccttctctctgttcttcttttactctcagatcaactccgatga
ggcaaaagtcatccgtcagtacagcttcatctttatcggtattactatcttcctgagcattctgactttcgttatcagc
cagttcctgctgaaagaaatgaagtacaagcgcaaccaagaagaaatcgagacttactacgagtacaccctgaagattg
aagcgatcaacaacgaaatgcgtaaatttcgtcacgactacgttaacattctgaccaccctgagcgaatacatccgtga
agacgacatgcctggcctgcgtgattatttcaacaaaaacattgtgccgatgaaagataacctgcaaatgaacgctatt
aagctgaacggtatcgaaaacctgaaagtacgtgagatcaagggtctgattaccgctaaaatcctgcgtgcgcaggaaa
tgaacatcccaattagcatcgaaatcccagacgaagtttctagcattaacctgaacatgattgacctgtctcgtagcat
cggcatcatcctggacaacgctatcgaagcttccactgaaatcgacgacccgattattcgcgttgcttttatcgagagc
gaaaactccgtgacctttatcgtgatgaacaaatgcgcggacgatatcccgcgtattcacgaactgttccaggaatctt
tcagcaccaagggtgaaggtcgcggcctgggcctgtccaccctgaaagagatcgcggataacgccgacaacgttctgct
ggacactattatcgaaaacggtttcttcattcagaaagtcgaaatcatcaataactaataaggatccaaagaggagaaa
tactagatggaaatcgcactggctacggacaatccatatgaggttctggaacaggctaaaaacatgaacgacatcggct
gctatttcctggatattcagctgagcaccgacatcaacggtatcaaactgggctctgaaatccgtaaacatgatccggt
tggtaacattattttcgtgacttctcattctgagctgacctatctgacgttcgtatacaaggttgcagccatggatttc
atctttaaagacgatccggcagagctgcgtacgcgcatcatcgactgtctggaaactgcgcacacgcgcctgcaactgc
tgtccaaagataactccgtggaaaccattgaactgaaacgtggcagcaacagcgtttacgttcagtacgatgatatcat
gttcttcgaaagctccactaagtcccaccgtctgatcgcgcatctggataatcgtcagatcgagttctatggcaacctg
aaagagctgagccagctggacgatcgcttcttccgttgtcataactccttcgttgttaaccgtcacaacatcgaatcca
tcgactccaaagagcgtattgtatacttcaagaacaaagaacactgttatgcgtctgtacgcaatgtcaagaaaatcta
ataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtc
ggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagattaaatac
aaattacatttaacagttaagtatttatttcctacagttaggcaatataatgataaaagattgtactaaatcgtataat
gacagtgaaggagaaatactagatggctagcatgcatcatcatcatcatcattcggactcagaagtcaatcaagaagct
aagccagaggtcaagccagaagtcaagcctgagactcacatcaatttaaaggtgtccgatggatcttcagagatcttct
tcaagatcaaaaagaccactcctttaagaaggctgatggaagcgttcgctaaacgtcagggtaaggaaatggactcctt
aagattcttgtacgacggtattaggattcaagctgatcagacccctgaagatttggacatggatgataacgatattatt
gaggctcacagagaacagaccggtggtatcatcggtcacctgatcaaaaccgcgctgggtatgctgggtctgtaataaa
gaggagaaatactagatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaa
tcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataa
gcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagc
tggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatt
taggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcact
agagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtc
gctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcacc
aaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgg
gtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagcca
ggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctcta
ctagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtccctatcagtgatagagattgac
atccctatcagtgatagagatactgagcactactagagaaagaggagaaatactagatgagcacaaaaaagaaaccatt
aacacaagagcagcttgaggacgcacgtcgccttaaagcaatttatgaaaaaaagaaaaatgaacttggcttatcccag
gaatctgtcgcagacaagatggggatggggcagtcaggcgttggtgctttatttaatggcatcaatgcattaaatgctt
ataacgccgcattgcttgcaaaaattctcaaagttagcgttgaagaatttagcccttcaatcgccagagaaatctacga
gatgtatgaagcggttagtatgcagccgtcacttagaagtgagtatgagtaccctgttttttctcatgttcaggcaggg
atgttctcacctgagcttagaacctttaccaaaggtgatgcggagagatgggtaagcacaaccaaaaaagccagtgatt
ctgcattctggcttgaggttgaaggtaattccatgaccgcaccaacaggctccaagccaagctttcctgacggaatgtt
aattctcgttgaccctgagcaggctgttgagccaggtgatttctgcatagccagacttgggggtgatgagtttaccttc
aagaaactgatcagggatagcggtcaggtgtttttacaaccactaaacccacagtacccaatgatcccatgcaatgaga
gttgttccgttgtggggaaagttatcgctagtcagtggcctgaagagacgtttggcgctgcaaacgacgaaaactacgc
tttagtagcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagt
cgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcggg
tgggcctttctgcgtttatatactagagtaacaccgtgcgtgttgactattttacctctggcggtgataatggttgctt
accggaaagaggagaaatactagatggctagccttgttcctgaattaaatgaaaaagacgatgaccaagtacaaaaagc
tttggcatcgagagaaaatactcagttaatgaatagagataatatagagataacagtacgtgattttaagaccttggca
ccacgaagatggctaaatgacactatcattgagttttttatgaaatacattgaaaaatctacccctaatacagtggcgt
ttaattcatttttctataccaatttatcagaaaggggttatcaaggcgtccggaggtggatgaagagaaagaagacaca
aattgataaacttgataaaatctttacaccaataaatttgaaccaatcccactgggcgttgggcataattgatttaaaa
aagaaaactataggttacgtagattcattatcgaatggtccaaatgctatgagtttcgctatactgactgacttgcaaa
aatatgttatggaggaaagtaagcatacaataggagaagactttgatttgattcatttagattgtccgcagcaaccaaa
tggctacgactgtggaatatatgtttgtatgaatactctctatggaagtgcagatgcgccattggattttgattataaa
gatgcgattaggatgagaagatttattgcccatttgattttaaccgacgctttaaaacgggatccgaactcgagctccg
tcgacaagcttgcggccgcactcgagcaccaccaccaccaccactgagtacattactagagcttaaaaggagggtctat
ggcagcacctagaatatcattttcgccctctgatattctatttggtgttctcgatcgcttgttcaaagataacgctacc
gggaaggttcttgcttcccgggtagctgtcgtaattcttttgtttataatggcgattgtttggtataggggagatagtt
tctttgagtactataagcaatcaaagtatgaaacatacagtgaaattattgaaaaggaaagaactgcacgctttgaatc
tgtcgccctggaacaactccagatagttcatatatcatctgaggcagactttagtgcggtgtattctttccgccctaaa
aacttaaactattttgttgatattatagcatacgaaggaaaattaccttcaacaataagtgaaaaatcacttggaggat
atcctgttgataaaactatggatgaatatacagttcatttaaatggacgtcattattattccaactcaaaatttgcttt
tttaccaactaaaaagcctactcccgaaataaactacatgtacagttgtccatattttaatttggataatatctatgct
ggaacgataaccatgtactggtatagaaatgatcatataagtaatgaccgccttgaatcaatatgtgctcaggcggcca
gaatattaggaagggctaaataattatactagagatacttaggaggtattatgaatatatttgaaatgttacgtataga
tgaaggtcttagacttaaaatctataaagacacagaaggctattacactattggcatcggtcatttgcttacaaaaagt
ccatcacttaatgctgctaaatctgaattagataaagctattgggcgtaattgcaatggtgtaattacaaaagatgagg
ctgaaaaactctttaatcaggatgttgatgctgctgttcgcggaatcctgagaaatgctaaattaaaaccggtttatga
ttctcttgatgcggttcgtcgctgtgcattgattaatatggttttccaaatgggagaaaccggtgtggcaggatttact
aactctttacgtatgcttcaacaaaaacgctgggatgaagcagcagttaacttagctaaaagtagatggtataatcaaa
cacctaatcgcgcaaaacgagtcattacaacgtttagaactggcacttgggacgcgtataaaaatctataaagctacta
gagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgc
tctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagttgacagctagctcagtc
ctagggactatgctagctactagaggataggaggcctttatggccttaaaagcaacagcactttttgccatgctaggat
tgtcatttgttttatctccatcgattgaagcgaatgtcgatcctcattttgataaatttatggaatctggtattaggca
cgtttatatgctttttgaaaataaaagcgtagaatcgtctgaacaattctatagttttatgagaacgacctataaaaat
gacccgtgctcttctgattttgaatgtatagagcgaggcgcggagatggcacaatcatacgctagaattatgaacatta
aattggagactgaatgaaattactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgtt
ttatctgttgtttgtcggtgaacgctctc