LOCUS BBa_K1022129 7163 bp DNA linear 05-Nov-2024 DEFINITION pBAD: Agr A : Agr C : pP2 : RBS: His6- SUMO: Magainin II:RBS TetR :TT : pTet: cI: TT : pcI: Ulp : Ly ACCESSION BBa_K1022129 VERSION BBa_K1022129.1 FEATURES Location/Qualifiers stem_loop 2078..2121 /label=stem_loop misc_feature 2067..2146 /label=BBa_B0010 terminator 2067..2146 /label=BBa_B0010 stem_loop 2162..2181 /label=T7 TE misc_feature 2188..2188 /label=stop polyA_site 2182..2195 /label=polya misc_feature 2155..2195 /label=BBa_B0012 terminator 2155..2195 /label=BBa_B0012 promoter 1..131 /label=promoter misc_feature 1..130 /label=BBa_K206000 misc_feature 40..57 /label=AraI1 misc_feature 61..78 /label=AraI2 misc_feature 139..2299 /label=BBa_I746103 misc_feature 139..2058 /label=BBa_I746100 RBS 139..150 /label=B0034-sequence RBS CDS 157..1404 /label=agrC coding sequence misc_feature 1405..1410 /label=BamHI site RBS 1411..1422 /label=B0034-sequence RBS CDS 1429..2058 /label=agrA coding sequence misc_feature 2067..2146 /label=BBa_B0010 stem_loop 2078..2121 /label=stem_loop misc_feature 2155..2195 /label=BBa_B0012 stem_loop 2162..2181 /label=T7 TE polyA_site 2182..2195 /label=polya misc_feature 2188..2188 /label=stop misc_feature 2204..2299 /label=BBa_I746104 misc_feature 1..2299 /label=BBa_K1022119 promoter 2300..2313 /label=pT7 misc_feature 2314..2634 /label=His 6 - SUMO misc_feature 2638..2707 /label=Magainin II RBS 2712..2715 /label=34 CDS 2729..3344 /label=Tet R misc_feature 3419..3498 /label=B0010 misc_feature 3507..3547 /label=B0012 misc_feature 1..3547 /label=BBa_K1022122 misc_feature 3556..3574 /label=TetR 1 misc_feature 3556..3609 /label=BBa_R0040 promoter 3575..3580 /label=-35 misc_feature 3581..3599 /label=TetR 2 promoter 3598..3603 /label=-10 promoter 3556..3609 /label=BBa_R0040 misc_feature 3622..3625 /label=conserved RBS 3618..3629 /label=BBa_B0034 promoter 3556..3629 /label=BBa_J13002 CDS 3639..4346 /label=cI lambda misc_feature 4347..4379 /label=LVA misc_feature 4386..4410 /label=Help:Barcodes CDS 3636..4410 /label=BBa_C0051 stem_loop 4430..4473 /label=stem_loop misc_feature 4419..4498 /label=BBa_B0010 terminator 4419..4498 /label=BBa_B0010 stem_loop 4514..4533 /label=T7 TE misc_feature 4540..4540 /label=stop polyA_site 4534..4547 /label=polya misc_feature 4507..4547 /label=BBa_B0012 terminator 4507..4547 /label=BBa_B0012 terminator 4419..4547 /label=BBa_B0015 misc_feature 3556..4547 /label=BBa_K1022108 promoter 4556..4604 /label=cI RBS 4613..4629 /label=34 misc_feature 4630..5370 /label=Ulp misc_feature 4556..5370 /label=BBa_K1022107 misc_feature 3556..5370 /label=BBa_K1022115 misc_feature 5379..7163 /label=BBa_K1022109 misc_feature 3556..7163 /label=BBa_K1022123 ORIGIN 1 acattgatta tttgcacggc gtcacacttt gctatgccat agcaagatag tccataagat 61 tagcggatcc tacctgacgc tttttatcgc aactctctac tgtttctcca taccgttttt 121 ttgggctagc tactagagaa agaggagaaa tactagatga ttctgatgtt caccattcca 181 gcaattatta gcggtattaa gtacagcaag ctggattatt tcttcatcat cgtaatctct 241 accctgtccc tgttcctgtt caaaatgttc gattccgcga gcctgattat cctgacttcc 301 ttcatcatca tcatgtattt cgtaaaaatc aaatggtata gcatcctgct gatcatgacc 361 tcccagatta tcctgtactg tgccaactac atgtatatcg ttatctacgc gtacatcacg 421 aaaatctccg attctatctt cgttatcttt ccgtctttct tcgtggtgta cgttacgatc 481 tctattctgt tttcctatat tatcaatcgc gttctgaaaa agatctctac tccgtacctg 541 attctgaaca aaggttttct gatcgtcatc tccactatcc tgctgctgac cttctctctg 601 ttcttctttt actctcagat caactccgat gaggcaaaag tcatccgtca gtacagcttc 661 atctttatcg gtattactat cttcctgagc attctgactt tcgttatcag ccagttcctg 721 ctgaaagaaa tgaagtacaa gcgcaaccaa gaagaaatcg agacttacta cgagtacacc 781 ctgaagattg aagcgatcaa caacgaaatg cgtaaatttc gtcacgacta cgttaacatt 841 ctgaccaccc tgagcgaata catccgtgaa gacgacatgc ctggcctgcg tgattatttc 901 aacaaaaaca ttgtgccgat gaaagataac ctgcaaatga acgctattaa gctgaacggt 961 atcgaaaacc tgaaagtacg tgagatcaag ggtctgatta ccgctaaaat cctgcgtgcg 1021 caggaaatga acatcccaat tagcatcgaa atcccagacg aagtttctag cattaacctg 1081 aacatgattg acctgtctcg tagcatcggc atcatcctgg acaacgctat cgaagcttcc 1141 actgaaatcg acgacccgat tattcgcgtt gcttttatcg agagcgaaaa ctccgtgacc 1201 tttatcgtga tgaacaaatg cgcggacgat atcccgcgta ttcacgaact gttccaggaa 1261 tctttcagca ccaagggtga aggtcgcggc ctgggcctgt ccaccctgaa agagatcgcg 1321 gataacgccg acaacgttct gctggacact attatcgaaa acggtttctt cattcagaaa 1381 gtcgaaatca tcaataacta ataaggatcc aaagaggaga aatactagat ggaaatcgca 1441 ctggctacgg acaatccata tgaggttctg gaacaggcta aaaacatgaa cgacatcggc 1501 tgctatttcc tggatattca gctgagcacc gacatcaacg gtatcaaact gggctctgaa 1561 atccgtaaac atgatccggt tggtaacatt attttcgtga cttctcattc tgagctgacc 1621 tatctgacgt tcgtatacaa ggttgcagcc atggatttca tctttaaaga cgatccggca 1681 gagctgcgta cgcgcatcat cgactgtctg gaaactgcgc acacgcgcct gcaactgctg 1741 tccaaagata actccgtgga aaccattgaa ctgaaacgtg gcagcaacag cgtttacgtt 1801 cagtacgatg atatcatgtt cttcgaaagc tccactaagt cccaccgtct gatcgcgcat 1861 ctggataatc gtcagatcga gttctatggc aacctgaaag agctgagcca gctggacgat 1921 cgcttcttcc gttgtcataa ctccttcgtt gttaaccgtc acaacatcga atccatcgac 1981 tccaaagagc gtattgtata cttcaagaac aaagaacact gttatgcgtc tgtacgcaat 2041 gtcaagaaaa tctaataata ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa 2101 agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctctact agagtcacac 2161 tggctcacct tcgggtgggc ctttctgcgt ttatatacta gagattaaat acaaattaca 2221 tttaacagtt aagtatttat ttcctacagt taggcaatat aatgataaaa gattgtacta 2281 aatcgtataa tgacagtgaa ggagaaatac tagatggcta gcatgcatca tcatcatcat 2341 cattcggact cagaagtcaa tcaagaagct aagccagagg tcaagccaga agtcaagcct 2401 gagactcaca tcaatttaaa ggtgtccgat ggatcttcag agatcttctt caagatcaaa 2461 aagaccactc ctttaagaag gctgatggaa gcgttcgcta aacgtcaggg taaggaaatg 2521 gactccttaa gattcttgta cgacggtatt aggattcaag ctgatcagac ccctgaagat 2581 ttggacatgg atgataacga tattattgag gctcacagag aacagaccgg tggtggtatc 2641 ggtaaattcc tgcactctgc gaaaaaattc ggtaaagcgt tcgttggtca gatcatgaac 2701 tcttaataaa gaggagaaat actagatgtc cagattagat aaaagtaaag tgattaacag 2761 cgcattagag ctgcttaatg aggtcggaat cgaaggttta acaacccgta aactcgccca 2821 gaagctaggt gtagagcagc ctacattgta ttggcatgta aaaaataagc gggctttgct 2881 cgacgcctta gccattgaga tgttagatag gcaccatact cacttttgcc ctttagaagg 2941 ggaaagctgg caagattttt tacgtaataa cgctaaaagt tttagatgtg ctttactaag 3001 tcatcgcgat ggagcaaaag tacatttagg tacacggcct acagaaaaac agtatgaaac 3061 tctcgaaaat caattagcct ttttatgcca acaaggtttt tcactagaga atgcattata 3121 tgcactcagc gctgtggggc attttacttt aggttgcgta ttggaagatc aagagcatca 3181 agtcgctaaa gaagaaaggg aaacacctac tactgatagt atgccgccat tattacgaca 3241 agctatcgaa ttatttgatc accaaggtgc agagccagcc ttcttattcg gccttgaatt 3301 gatcatatgc ggattagaaa aacaacttaa atgtgaaagt gggtccgctg caaacgacga 3361 aaactacgct ttagtagctt aataacactg atagtgctag tgtagatcac tactagagcc 3421 aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt 3481 ttgtcggtga acgctctcta ctagagtcac actggctcac cttcgggtgg gcctttctgc 3541 gtttatatac tagagtccct atcagtgata gagattgaca tccctatcag tgatagagat 3601 actgagcact actagagaaa gaggagaaat actagatgag cacaaaaaag aaaccattaa 3661 cacaagagca gcttgaggac gcacgtcgcc ttaaagcaat ttatgaaaaa aagaaaaatg 3721 aacttggctt atcccaggaa tctgtcgcag acaagatggg gatggggcag tcaggcgttg 3781 gtgctttatt taatggcatc aatgcattaa atgcttataa cgccgcattg cttgcaaaaa 3841 ttctcaaagt tagcgttgaa gaatttagcc cttcaatcgc cagagaaatc tacgagatgt 3901 atgaagcggt tagtatgcag ccgtcactta gaagtgagta tgagtaccct gttttttctc 3961 atgttcaggc agggatgttc tcacctgagc ttagaacctt taccaaaggt gatgcggaga 4021 gatgggtaag cacaaccaaa aaagccagtg attctgcatt ctggcttgag gttgaaggta 4081 attccatgac cgcaccaaca ggctccaagc caagctttcc tgacggaatg ttaattctcg 4141 ttgaccctga gcaggctgtt gagccaggtg atttctgcat agccagactt gggggtgatg 4201 agtttacctt caagaaactg atcagggata gcggtcaggt gtttttacaa ccactaaacc 4261 cacagtaccc aatgatccca tgcaatgaga gttgttccgt tgtggggaaa gttatcgcta 4321 gtcagtggcc tgaagagacg tttggcgctg caaacgacga aaactacgct ttagtagctt 4381 aataacgctg atagtgctag tgtagatcgc tactagagcc aggcatcaaa taaaacgaaa 4441 ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcta 4501 ctagagtcac actggctcac cttcgggtgg gcctttctgc gtttatatac tagagtaaca 4561 ccgtgcgtgt tgactatttt acctctggcg gtgataatgg ttgcttaccg gaaagaggag 4621 aaatactaga tggctagcct tgttcctgaa ttaaatgaaa aagacgatga ccaagtacaa 4681 aaagctttgg catcgagaga aaatactcag ttaatgaata gagataatat agagataaca 4741 gtacgtgatt ttaagacctt ggcaccacga agatggctaa atgacactat cattgagttt 4801 tttatgaaat acattgaaaa atctacccct aatacagtgg cgtttaattc atttttctat 4861 accaatttat cagaaagggg ttatcaaggc gtccggaggt ggatgaagag aaagaagaca 4921 caaattgata aacttgataa aatctttaca ccaataaatt tgaaccaatc ccactgggcg 4981 ttgggcataa ttgatttaaa aaagaaaact ataggttacg tagattcatt atcgaatggt 5041 ccaaatgcta tgagtttcgc tatactgact gacttgcaaa aatatgttat ggaggaaagt 5101 aagcatacaa taggagaaga ctttgatttg attcatttag attgtccgca gcaaccaaat 5161 ggctacgact gtggaatata tgtttgtatg aatactctct atggaagtgc agatgcgcca 5221 ttggattttg attataaaga tgcgattagg atgagaagat ttattgccca tttgatttta 5281 accgacgctt taaaacggga tccgaactcg agctccgtcg acaagcttgc ggccgcactc 5341 gagcaccacc accaccacca ctgagtacat tactagagct taaaaggagg gtctatggca 5401 gcacctagaa tatcattttc gccctctgat attctatttg gtgttctcga tcgcttgttc 5461 aaagataacg ctaccgggaa ggttcttgct tcccgggtag ctgtcgtaat tcttttgttt 5521 ataatggcga ttgtttggta taggggagat agtttctttg agtactataa gcaatcaaag 5581 tatgaaacat acagtgaaat tattgaaaag gaaagaactg cacgctttga atctgtcgcc 5641 ctggaacaac tccagatagt tcatatatca tctgaggcag actttagtgc ggtgtattct 5701 ttccgcccta aaaacttaaa ctattttgtt gatattatag catacgaagg aaaattacct 5761 tcaacaataa gtgaaaaatc acttggagga tatcctgttg ataaaactat ggatgaatat 5821 acagttcatt taaatggacg tcattattat tccaactcaa aatttgcttt tttaccaact 5881 aaaaagccta ctcccgaaat aaactacatg tacagttgtc catattttaa tttggataat 5941 atctatgctg gaacgataac catgtactgg tatagaaatg atcatataag taatgaccgc 6001 cttgaatcaa tatgtgctca ggcggccaga atattaggaa gggctaaata attatactag 6061 agatacttag gaggtattat gaatatattt gaaatgttac gtatagatga aggtcttaga 6121 cttaaaatct ataaagacac agaaggctat tacactattg gcatcggtca tttgcttaca 6181 aaaagtccat cacttaatgc tgctaaatct gaattagata aagctattgg gcgtaattgc 6241 aatggtgtaa ttacaaaaga tgaggctgaa aaactcttta atcaggatgt tgatgctgct 6301 gttcgcggaa tcctgagaaa tgctaaatta aaaccggttt atgattctct tgatgcggtt 6361 cgtcgctgtg cattgattaa tatggttttc caaatgggag aaaccggtgt ggcaggattt 6421 actaactctt tacgtatgct tcaacaaaaa cgctgggatg aagcagcagt taacttagct 6481 aaaagtagat ggtataatca aacacctaat cgcgcaaaac gagtcattac aacgtttaga 6541 actggcactt gggacgcgta taaaaatcta taaagctact agagccaggc atcaaataaa 6601 acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc 6661 tctctactag agtcacactg gctcaccttc gggtgggcct ttctgcgttt atatactaga 6721 gttgacagct agctcagtcc tagggactat gctagctact agaggatagg aggcctttat 6781 ggccttaaaa gcaacagcac tttttgccat gctaggattg tcatttgttt tatctccatc 6841 gattgaagcg aatgtcgatc ctcattttga taaatttatg gaatctggta ttaggcacgt 6901 ttatatgctt tttgaaaata aaagcgtaga atcgtctgaa caattctata gttttatgag 6961 aacgacctat aaaaatgacc cgtgctcttc tgattttgaa tgtatagagc gaggcgcgga 7021 gatggcacaa tcatacgcta gaattatgaa cattaaattg gagactgaat gaaattacta 7081 gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct 7141 gttgtttgtc ggtgaacgct ctc //