##gff-version 3
##sequence-region BBa_K1022129 1 7163
BBa_K1022129	.	engineered_region	3556	7163	.	+	0	ID=BBa_K1022123;Name=BBa_K1022123
BBa_K1022129	.	engineered_region	3556	5370	.	+	0	ID=BBa_K1022115;Name=BBa_K1022115;Parent=BBa_K1022123
BBa_K1022129	.	sequence_feature	4556	5370	.	+	0	ID=BBa_K1022107;Name=BBa_K1022107;Parent=BBa_K1022115
BBa_K1022129	.	sequence_feature	4630	5370	.	+	0	ID=annotation2335076;Name=Ulp;Parent=BBa_K1022107
BBa_K1022129	.	promoter	4556	4604	.	+	0	ID=annotation2335074;Name=cI;Parent=BBa_K1022107
BBa_K1022129	.	ribosome_entry_site	4613	4629	.	+	0	ID=annotation2335075;Name=34;Parent=BBa_K1022107
BBa_K1022129	.	engineered_region	3556	4547	.	+	0	ID=BBa_K1022108;Name=BBa_K1022108;Parent=BBa_K1022115
BBa_K1022129	.	CDS	3636	4410	.	+	0	ID=BBa_C0051;Name=BBa_C0051;Parent=BBa_K1022108
BBa_K1022129	.	tag	4347	4379	.	+	0	ID=annotation23335;Name=LVA;Parent=BBa_C0051
BBa_K1022129	.	CDS	3639	4346	.	+	0	ID=annotation23334;Name=cI lambda;Parent=BBa_C0051
BBa_K1022129	.	engineered_tag	4386	4410	.	+	0	ID=annotation2213991;Name=Help:Barcodes;Parent=BBa_C0051
BBa_K1022129	.	terminator	4419	4547	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K1022108
BBa_K1022129	.	terminator	4419	4498	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K1022129	.	stem_loop	4430	4473	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1022129	.	engineered_region	4419	4498	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1022129	.	terminator	4507	4547	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K1022129	.	engineered_region	4507	4547	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1022129	.	stop_codon	4540	4540	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1022129	.	stem_loop	4514	4533	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1022129	.	polyA_site	4534	4547	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1022129	.	promoter	3556	3629	.	+	0	ID=BBa_J13002;Name=BBa_J13002;Parent=BBa_K1022108
BBa_K1022129	.	promoter	3556	3609	.	+	0	ID=BBa_R0040;Name=BBa_R0040;Parent=BBa_J13002
BBa_K1022129	.	promoter	3575	3580	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_K1022129	.	non_covalent_binding_site	3581	3599	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_K1022129	.	engineered_region	3556	3609	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_K1022129	.	non_covalent_binding_site	3556	3574	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_K1022129	.	promoter	3598	3603	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_K1022129	.	ribosome_entry_site	3618	3629	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_J13002
BBa_K1022129	.	conserved_region	3622	3625	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1022129	.	engineered_region	5379	7163	.	+	0	ID=BBa_K1022109;Name=BBa_K1022109;Parent=BBa_K1022123
BBa_K1022129	.	terminator	2067	2146	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_K1022129	.	stem_loop	2078	2121	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1022129	.	engineered_region	2067	2146	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1022129	.	engineered_region	1	3547	.	+	0	ID=BBa_K1022122;Name=BBa_K1022122
BBa_K1022129	.	stop_codon	3507	3547	.	+	0	ID=annotation2356702;Name=B0012;Parent=BBa_K1022122
BBa_K1022129	.	engineered_region	139	2299	.	+	0	ID=annotation2356573;Name=BBa_I746103;Parent=BBa_K1022122
BBa_K1022129	.	engineered_region	1	130	.	+	0	ID=annotation2356570;Name=BBa_K206000;Parent=BBa_K1022122
BBa_K1022129	.	CDS	157	1404	.	+	0	ID=annotation2356576;Name=agrC coding sequence;Parent=BBa_K1022122
BBa_K1022129	.	promoter	1	131	.	+	0	ID=annotation2356569;Name=promoter;Parent=BBa_K1022122
BBa_K1022129	.	tag	2314	2634	.	+	0	ID=annotation2356589;Name=His 6 - SUMO;Parent=BBa_K1022122
BBa_K1022129	.	stem_loop	2078	2121	.	+	0	ID=annotation2356581;Name=stem_loop;Parent=BBa_K1022122
BBa_K1022129	.	ribosome_entry_site	1411	1422	.	+	0	ID=annotation2356578;Name=B0034-sequence RBS;Parent=BBa_K1022122
BBa_K1022129	.	sequence_feature	2638	2707	.	+	0	ID=annotation2356590;Name=Magainin II;Parent=BBa_K1022122
BBa_K1022129	.	ribosome_entry_site	2712	2715	.	+	0	ID=annotation2356651;Name=34;Parent=BBa_K1022122
BBa_K1022129	.	CDS	1429	2058	.	+	0	ID=annotation2356579;Name=agrA coding sequence;Parent=BBa_K1022122
BBa_K1022129	.	promoter	2300	2313	.	+	0	ID=annotation2356588;Name=pT7;Parent=BBa_K1022122
BBa_K1022129	.	operator	40	57	.	+	0	ID=annotation2356571;Name=AraI1;Parent=BBa_K1022122
BBa_K1022129	.	polyA_site	2182	2195	.	+	0	ID=annotation2356584;Name=polya;Parent=BBa_K1022122
BBa_K1022129	.	sequence_feature	1405	1410	.	+	0	ID=annotation2356577;Name=BamHI site;Parent=BBa_K1022122
BBa_K1022129	.	engineered_region	1	2299	.	+	0	ID=annotation2356587;Name=BBa_K1022119;Parent=BBa_K1022122
BBa_K1022129	.	CDS	2729	3344	.	+	0	ID=annotation2356652;Name=Tet R;Parent=BBa_K1022122
BBa_K1022129	.	engineered_region	139	2058	.	+	0	ID=annotation2356574;Name=BBa_I746100;Parent=BBa_K1022122
BBa_K1022129	.	stop_codon	3419	3498	.	+	0	ID=annotation2356684;Name=B0010;Parent=BBa_K1022122
BBa_K1022129	.	engineered_region	2204	2299	.	+	0	ID=annotation2356586;Name=BBa_I746104;Parent=BBa_K1022122
BBa_K1022129	.	engineered_region	2155	2195	.	+	0	ID=annotation2356582;Name=BBa_B0012;Parent=BBa_K1022122
BBa_K1022129	.	engineered_region	2067	2146	.	+	0	ID=annotation2356580;Name=BBa_B0010;Parent=BBa_K1022122
BBa_K1022129	.	ribosome_entry_site	139	150	.	+	0	ID=annotation2356575;Name=B0034-sequence RBS;Parent=BBa_K1022122
BBa_K1022129	.	stop_codon	2188	2188	.	+	0	ID=annotation2356585;Name=stop;Parent=BBa_K1022122
BBa_K1022129	.	operator	61	78	.	+	0	ID=annotation2356572;Name=AraI2;Parent=BBa_K1022122
BBa_K1022129	.	stem_loop	2162	2181	.	+	0	ID=annotation2356583;Name=T7 TE;Parent=BBa_K1022122
BBa_K1022129	.	terminator	2155	2195	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_K1022129	.	engineered_region	2155	2195	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1022129	.	stop_codon	2188	2188	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1022129	.	stem_loop	2162	2181	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1022129	.	polyA_site	2182	2195	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
>BBa_K1022129
acattgattatttgcacggcgtcacactttgctatgccatagcaagatagtccataagattagcggatcctacctgacg
ctttttatcgcaactctctactgtttctccataccgtttttttgggctagctactagagaaagaggagaaatactagat
gattctgatgttcaccattccagcaattattagcggtattaagtacagcaagctggattatttcttcatcatcgtaatc
tctaccctgtccctgttcctgttcaaaatgttcgattccgcgagcctgattatcctgacttccttcatcatcatcatgt
atttcgtaaaaatcaaatggtatagcatcctgctgatcatgacctcccagattatcctgtactgtgccaactacatgta
tatcgttatctacgcgtacatcacgaaaatctccgattctatcttcgttatctttccgtctttcttcgtggtgtacgtt
acgatctctattctgttttcctatattatcaatcgcgttctgaaaaagatctctactccgtacctgattctgaacaaag
gttttctgatcgtcatctccactatcctgctgctgaccttctctctgttcttcttttactctcagatcaactccgatga
ggcaaaagtcatccgtcagtacagcttcatctttatcggtattactatcttcctgagcattctgactttcgttatcagc
cagttcctgctgaaagaaatgaagtacaagcgcaaccaagaagaaatcgagacttactacgagtacaccctgaagattg
aagcgatcaacaacgaaatgcgtaaatttcgtcacgactacgttaacattctgaccaccctgagcgaatacatccgtga
agacgacatgcctggcctgcgtgattatttcaacaaaaacattgtgccgatgaaagataacctgcaaatgaacgctatt
aagctgaacggtatcgaaaacctgaaagtacgtgagatcaagggtctgattaccgctaaaatcctgcgtgcgcaggaaa
tgaacatcccaattagcatcgaaatcccagacgaagtttctagcattaacctgaacatgattgacctgtctcgtagcat
cggcatcatcctggacaacgctatcgaagcttccactgaaatcgacgacccgattattcgcgttgcttttatcgagagc
gaaaactccgtgacctttatcgtgatgaacaaatgcgcggacgatatcccgcgtattcacgaactgttccaggaatctt
tcagcaccaagggtgaaggtcgcggcctgggcctgtccaccctgaaagagatcgcggataacgccgacaacgttctgct
ggacactattatcgaaaacggtttcttcattcagaaagtcgaaatcatcaataactaataaggatccaaagaggagaaa
tactagatggaaatcgcactggctacggacaatccatatgaggttctggaacaggctaaaaacatgaacgacatcggct
gctatttcctggatattcagctgagcaccgacatcaacggtatcaaactgggctctgaaatccgtaaacatgatccggt
tggtaacattattttcgtgacttctcattctgagctgacctatctgacgttcgtatacaaggttgcagccatggatttc
atctttaaagacgatccggcagagctgcgtacgcgcatcatcgactgtctggaaactgcgcacacgcgcctgcaactgc
tgtccaaagataactccgtggaaaccattgaactgaaacgtggcagcaacagcgtttacgttcagtacgatgatatcat
gttcttcgaaagctccactaagtcccaccgtctgatcgcgcatctggataatcgtcagatcgagttctatggcaacctg
aaagagctgagccagctggacgatcgcttcttccgttgtcataactccttcgttgttaaccgtcacaacatcgaatcca
tcgactccaaagagcgtattgtatacttcaagaacaaagaacactgttatgcgtctgtacgcaatgtcaagaaaatcta
ataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtc
ggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagattaaatac
aaattacatttaacagttaagtatttatttcctacagttaggcaatataatgataaaagattgtactaaatcgtataat
gacagtgaaggagaaatactagatggctagcatgcatcatcatcatcatcattcggactcagaagtcaatcaagaagct
aagccagaggtcaagccagaagtcaagcctgagactcacatcaatttaaaggtgtccgatggatcttcagagatcttct
tcaagatcaaaaagaccactcctttaagaaggctgatggaagcgttcgctaaacgtcagggtaaggaaatggactcctt
aagattcttgtacgacggtattaggattcaagctgatcagacccctgaagatttggacatggatgataacgatattatt
gaggctcacagagaacagaccggtggtggtatcggtaaattcctgcactctgcgaaaaaattcggtaaagcgttcgttg
gtcagatcatgaactcttaataaagaggagaaatactagatgtccagattagataaaagtaaagtgattaacagcgcat
tagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctac
attgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcac
ttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtc
atcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctt
tttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgta
ttggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgac
aagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattaga
aaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgc
tagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctg
ttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagag
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatacta
gatgagcacaaaaaagaaaccattaacacaagagcagcttgaggacgcacgtcgccttaaagcaatttatgaaaaaaag
aaaaatgaacttggcttatcccaggaatctgtcgcagacaagatggggatggggcagtcaggcgttggtgctttattta
atggcatcaatgcattaaatgcttataacgccgcattgcttgcaaaaattctcaaagttagcgttgaagaatttagccc
ttcaatcgccagagaaatctacgagatgtatgaagcggttagtatgcagccgtcacttagaagtgagtatgagtaccct
gttttttctcatgttcaggcagggatgttctcacctgagcttagaacctttaccaaaggtgatgcggagagatgggtaa
gcacaaccaaaaaagccagtgattctgcattctggcttgaggttgaaggtaattccatgaccgcaccaacaggctccaa
gccaagctttcctgacggaatgttaattctcgttgaccctgagcaggctgttgagccaggtgatttctgcatagccaga
cttgggggtgatgagtttaccttcaagaaactgatcagggatagcggtcaggtgtttttacaaccactaaacccacagt
acccaatgatcccatgcaatgagagttgttccgttgtggggaaagttatcgctagtcagtggcctgaagagacgtttgg
cgctgcaaacgacgaaaactacgctttagtagcttaataacgctgatagtgctagtgtagatcgctactagagccaggc
atcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctacta
gagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtaacaccgtgcgtgttgactattttac
ctctggcggtgataatggttgcttaccggaaagaggagaaatactagatggctagccttgttcctgaattaaatgaaaa
agacgatgaccaagtacaaaaagctttggcatcgagagaaaatactcagttaatgaatagagataatatagagataaca
gtacgtgattttaagaccttggcaccacgaagatggctaaatgacactatcattgagttttttatgaaatacattgaaa
aatctacccctaatacagtggcgtttaattcatttttctataccaatttatcagaaaggggttatcaaggcgtccggag
gtggatgaagagaaagaagacacaaattgataaacttgataaaatctttacaccaataaatttgaaccaatcccactgg
gcgttgggcataattgatttaaaaaagaaaactataggttacgtagattcattatcgaatggtccaaatgctatgagtt
tcgctatactgactgacttgcaaaaatatgttatggaggaaagtaagcatacaataggagaagactttgatttgattca
tttagattgtccgcagcaaccaaatggctacgactgtggaatatatgtttgtatgaatactctctatggaagtgcagat
gcgccattggattttgattataaagatgcgattaggatgagaagatttattgcccatttgattttaaccgacgctttaa
aacgggatccgaactcgagctccgtcgacaagcttgcggccgcactcgagcaccaccaccaccaccactgagtacatta
ctagagcttaaaaggagggtctatggcagcacctagaatatcattttcgccctctgatattctatttggtgttctcgat
cgcttgttcaaagataacgctaccgggaaggttcttgcttcccgggtagctgtcgtaattcttttgtttataatggcga
ttgtttggtataggggagatagtttctttgagtactataagcaatcaaagtatgaaacatacagtgaaattattgaaaa
ggaaagaactgcacgctttgaatctgtcgccctggaacaactccagatagttcatatatcatctgaggcagactttagt
gcggtgtattctttccgccctaaaaacttaaactattttgttgatattatagcatacgaaggaaaattaccttcaacaa
taagtgaaaaatcacttggaggatatcctgttgataaaactatggatgaatatacagttcatttaaatggacgtcatta
ttattccaactcaaaatttgcttttttaccaactaaaaagcctactcccgaaataaactacatgtacagttgtccatat
tttaatttggataatatctatgctggaacgataaccatgtactggtatagaaatgatcatataagtaatgaccgccttg
aatcaatatgtgctcaggcggccagaatattaggaagggctaaataattatactagagatacttaggaggtattatgaa
tatatttgaaatgttacgtatagatgaaggtcttagacttaaaatctataaagacacagaaggctattacactattggc
atcggtcatttgcttacaaaaagtccatcacttaatgctgctaaatctgaattagataaagctattgggcgtaattgca
atggtgtaattacaaaagatgaggctgaaaaactctttaatcaggatgttgatgctgctgttcgcggaatcctgagaaa
tgctaaattaaaaccggtttatgattctcttgatgcggttcgtcgctgtgcattgattaatatggttttccaaatggga
gaaaccggtgtggcaggatttactaactctttacgtatgcttcaacaaaaacgctgggatgaagcagcagttaacttag
ctaaaagtagatggtataatcaaacacctaatcgcgcaaaacgagtcattacaacgtttagaactggcacttgggacgc
gtataaaaatctataaagctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttt
tatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatata
ctagagttgacagctagctcagtcctagggactatgctagctactagaggataggaggcctttatggccttaaaagcaa
cagcactttttgccatgctaggattgtcatttgttttatctccatcgattgaagcgaatgtcgatcctcattttgataa
atttatggaatctggtattaggcacgtttatatgctttttgaaaataaaagcgtagaatcgtctgaacaattctatagt
tttatgagaacgacctataaaaatgacccgtgctcttctgattttgaatgtatagagcgaggcgcggagatggcacaat
catacgctagaattatgaacattaaattggagactgaatgaaattactagagccaggcatcaaataaaacgaaaggctc
agtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc