BBa_K1023017 1 BBa_K1023017 eForRed Chromoprotein 2013-12-19T12:00:00Z 2015-05-08T01:08:45Z The XL1 Blue MRF??? E. coli strains which contain plasmids with chromoprotein encoding genes aeBlue, eforRed and amilGFP respectively and DH5α E. coli strain containing mRFP were obtained from Braunschweig UR and Uppsala UR. We synthesized and via Gibson assembly put together a chromoprotein encoding gene (eforRed) which was codon-optimized for Aspergillus niger. A mitochondrial retention signal was added to this codon-optimized chromoprotein encoding gene for targeted organelle localization. There is a Nsi restriction site in the middle that allows one to cleave of the signal peptide if required. 5'-signal seq - Nsi1 - eforRed-3' false false _1330_ 0 15730 9 It's complicated false codon bias false Michiel Karrenbelt annotation2371758 1 Nsi1 range2371758 1 73 79 BBa_K1023017_sequence 1 atgattaccacaaggcttgcgcgcatgggtgcgctggcccctaagtcccgccttatgctcggggctcggggtatgcatatgtcggtaatcaagcaagtgatgaaaactaagcttcatcttgaaggtaccgttaatggccatgattttaccatcgagggtaaaggcgaaggcaaaccttacgaaggtcttcagcatatgaagatgacagttaccaaaggtgcgccactgcccttctcagtacatatcctgaccccttcacacatgtatggttcaaagccatttaataagtacccggctgacatcccggattaccacaaacagtcgttcccagagggcatgtcctgggaaagatccatgatcttcgactcgtcgatcaacctacaggaaaattgctttatttatgatgtcaagtttcacggtgtgaatttgccacctgatggcccggtcatgcagaagacgatcgctggttgggagccttccgtcgaaactctgtacgtccgagatgggatgcttaagtctgacacagcaatggtgttcaaattgaaagggggaggtcaccatagggtggattttaagaccacctacaaggctaagaagccagtgaagctgcctgagtttcattttgtggaacaccgcctcgaactgaccaagcacgataaggatttcaccacttgggatcaacaagaggcagcggagggccatttctcccctctcccgaaggctctcccgtaa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z