LOCUS BBa_K1024003 4480 bp DNA linear 22-Dec-2024 DEFINITION Reporter for Tet system in Yeast ACCESSION BBa_K1024003 VERSION BBa_K1024003.1 FEATURES Location/Qualifiers CDS 1..387 /label=VP16 misc_feature 388..1008 /label=TetR misc_feature 1317..1696 /label=CMV Enhancer promoter 1113..1316 /label=pCMV misc_feature 2025..2295 /label=TetO promoter 2322..2467 /label=Cyc TATA Region CDS 2486..4200 /label=ADE2 misc_feature 4233..4480 /label=CYC1 Terminator ORIGIN 1 ctacccaccg tactcgtcaa ttccaagggc atcggtaaac atctgctcaa actcgaagtc 61 ggccatatcc agagcgccgt agggggcgga gtcgtggggg gtaaatcccg gacccgggga 121 atccccgtcc cccaacatgt ccagatcgaa atcgtctagc gcgtcggcat gcgccatcgc 181 cacgtcctcg ccgtctaagt ggagctcgtc ccccaggctg acatcggtcg ggggggccgt 241 cgacagtctg cgcgtgtgtc ccgcggggag aaaggacagg cgcggagccg ccagccccgc 301 ctcttcgggg gcgtcgtcgt ccgggagatc gagcaggccc tcgatggtag acccgtaatt 361 gtttttcgta cgcgcgcggc tgtacgcgga cccactttca catttaagtt gtttttctaa 421 tccgcatatg atcaattcaa ggccgaataa gaaggctggc tctgcacctt ggtgatcaaa 481 taattcgata gcttgtcgta ataatggcgg catactatca gtagtaggtg tttccctttc 541 ttctttagcg acttgatgct cttgatcttc caatacgcaa cctaaagtaa aatgccccac 601 agcgctgagt gcatataatg cattctctag tgaaaaacct tgttggcata aaaaggctaa 661 ttgattttcg agagtttcat actgtttttc tgtaggccgt gtacctaaat gtacttttgc 721 tccatcgcga tgacttagta aagcacatct aaaactttta gcgttattac gtaaaaaatc 781 ttgccagctt tccccttcta aagggcaaaa gtgagtatgg tgcctatcta acatctcaat 841 ggctaaggcg tcgagcaaag cccgcttatt ttttacatgc caatacaatg taggctgctc 901 tacacctagc ttctgggcga gtttacgggt tgttaaacct tcgattccga cctcattaag 961 cagctctaat gcgctgttaa tcactttact tttatctaat ttagacatat gaattaattc 1021 gggccgcgga ggctggatcg gtcccggtgt cttctatgga ggtcaaaaca gcgtggatgg 1081 cgtctccagg cgatctgacg gttcactaaa cgagctctgc ttatatagac ctcccaccgt 1141 acacgcctac cgcccatttg cgtcaatggg gcggagttgt tacgacattt tggaaagtcc 1201 cgttgatttt ggtgccaaaa caaactccca ttgacgtcaa tggggtggag acttggaaat 1261 ccccgtgagt caaaccgcta tccacgccca ttgatgtact gccaaaaccg catcaccatg 1321 gtaatagcga tgactaatac gtagatgtac tgccaagtag gaaagtccca taaggtcatg 1381 tactgggcat aatgccaggc gggccattta ccgtcattga cgtcaatagg gggcgtactt 1441 ggcatatgat acacttgatg tactgccaag tgggcagttt agcgtaaata ctccacccat 1501 tgacgtcaat ggaaagtccc tattggcgtt actatgggaa catacgtcat tattgacgtc 1561 aatgggcggg ggtcgttggg cggtcagcca ggcgggccat ttaccgtaag ttatgtaacg 1621 cggaactcca tatatgggct atgaactaat gaccccgtaa ttgattacta ttaatatcta 1681 gtcaataatc aatgtcaaca tggcggtaat gttggacatg agccaatata aatgtacata 1741 ttatgatatg gatacaacgt atgcaatggg ccaagctcct cgagtaattc gcgccacttc 1801 taaataagcg aatttcttat gatttatgat ttttattatt aaataagtta taaaaaaaat 1861 aagtgtatac aaattttaaa gtgactctta ggttttaaaa cgaaaattct tgttcttgag 1921 taactctttc ctgtaggtca ggttgctttc tcaggtatag catgaggtcg ctcttattga 1981 ccacacctct accggcagat caattcctcg atcgagttta ccactcccta tcagtgatag 2041 agaaaagtga aagtcgagtt taccactccc tatcagtgat agagaaaagt gaaagtcgag 2101 tttaccactc cctatcagtg atagagaaaa gtgaaagtcg agtttaccac tccctatcag 2161 tgatagagaa aagtgaaagt cgagtttacc actccctatc agtgatagag aaaagtgaaa 2221 gtcgagttta ccactcccta tcagtgatag agaaaagtga aagtcgagtt taccactccc 2281 tatcagtgat agagaaaagt gaaagtcgag ctcggtaccc tatggcatgc atgtgctctg 2341 tatgtatata aaactcttgt tttcttcttt tctctaaata ttctttcctt atacattagg 2401 tcctttgtag cataaattac tatacttcta tagacacgca aacacaaata cacacactaa 2461 attaccggat caattcgggg gatccatgga ttttagaaca gttggtatat taggaggggg 2521 acaattggga cgtatgattg ttgaggcagc aaacaggctc aacattaaga cggtaatact 2581 agatgctgaa aattctcctg ccaaacaaat aagcaactcc aatgaccacg ttaatggctc 2641 cttttccaat cctcttgata tcgaaaaact agctgaaaaa tgtgatgtgc taacgattga 2701 gattgagcat gttgatgttc ctacactaaa gaatcttcaa gtaaaacatc ccaaattaaa 2761 aatttaccct tctccagaaa caatcagatt gatacaagac aaatatattc aaaaagagca 2821 tttaatcaaa aatggtatag cagttaccca aagtgttcct gtggaacaag ccagtgagac 2881 gtccctattg aatgttggaa gagatttggg ttttccattc gtcttgaagt cgaggacttt 2941 ggcatacgat ggaagaggta acttcgttgt aaagaataag gaaatgattc cggaagcttt 3001 ggaagtactg aaggatcgtc ctttgtacgc cgaaaaatgg gcaccattta ctaaagaatt 3061 agcagtcatg attgtgagat ctgttaacgg tttagtgttt tcttacccaa ttgtagagac 3121 tatccacaag gacaatattt gtgacttatg ttatgcgcct gctagagttc cggactccgt 3181 tcaacttaag gcgaagttgt tggcagaaaa tgcaatcaaa tcttttcccg gttgtggtat 3241 atttggtgtg gaaatgttct atttagaaac aggggaattg cttattaacg aaattgcccc 3301 aaggcctcac aactctggac attataccat tgatgcttgc gtcacttctc aatttgaagc 3361 tcatttgaga tcaatattgg atttgccaat gccaaagaat ttcacatctt tctccaccat 3421 tacaacgaac gccattatgc taaatgttct tggagacaaa catacaaaag ataaagagct 3481 agaaacttgc gaaagagcat tggcgactcc aggttcctca gtgtacttat atggaaaaga 3541 gtttagacct aacagaaaag taggtcacat aaatattatt gcctccagta tggcggaatg 3601 tgaacaaagg ctgaactaca ttacaggtag aactgatatt ccaatcaaaa tctctgtcgc 3661 tcaaaagttg gacttggaag caatggtcaa accattggtt ggaatcatca tgggatcaga 3721 ctctgacttg ccggtaatgt ctgccgcatg tgcggtttta aaagattttg gcgttccatt 3781 tgaagtgaca atagtctctg ctcatagaac tccacatagg atgtcagcat atgctatttc 3841 cgcaagcaag cgtggaatta aaacaattat cgctggagct ggtggggctg ctcacttgcc 3901 aggtatggtg gctgcaatga caccacttcc tgtcatcggt gtgcccgtaa aaggttcttg 3961 tttagatgga gtagattctt tacattcaat tgtgcaaatg cctagaggtg ttccagtagc 4021 taccgtcgct attaataata gtacgaacgc tgcgctgttg gctgtcagac tgcttggcgc 4081 ttatgattca agttatacaa cgaaaatgga acagttttta ttaaagcaag aagaagaagt 4141 tcttgtcaaa gcacaaaagt tagaaactgt cggttacgaa gcttatttag aaaacaagta 4201 agctgccgct agggccctgc atgagggccg catcatgtaa ttagttatgt cacgcttaca 4261 ttcacgccct ccccccacat ccgctctaac cgaaaaggaa ggagttagac aacctgaagt 4321 ctaggtccct atttattttt ttatagttat gttagtatta agaacgttat ttatatttca 4381 aatttttctt ttttttctgt acagacgcgt gtacgcatgt aacattatac tgaaaacctt 4441 gcttgagaag gttttgggac gctcgaaggc tttaatttgc //