LOCUS       BBa_K1024004            3559 bp    DNA     linear       22-Nov-2024
DEFINITION  Sensor yeast (inducible TetR+VP16)
ACCESSION   BBa_K1024004
VERSION     BBa_K1024004.1  
FEATURES             Location/Qualifiers
     promoter        1..379
                     /label=pTEF
     misc_feature    395..418
                     /label=Flag
     misc_feature    1..756
                     /label=BBa_C0062
     misc_feature    419..1579
                     /label=VP16
     misc_feature    1607..2387
                     /label=LuxR
     promoter        2443..2546
                     /label=CYC 100 Mini
     promoter        2388..2442
                     /label=pLux
     misc_feature    2552..3172
                     /label=TetR
     CDS             3173..3559
                     /label=VP16
ORIGIN
        1 agcttgcctt gtccccgccg ggtcacccgg ccagcgacat ggaggcccag aataccctcc
       61 ttgacagtct tgacgtgcgc agctcagggg catgatgtga ctgtcgcccg tacatttagc
      121 ccatacatcc ccatgtataa tcatttgcat ccatacattt tgatggccgc acggcgcgaa
      181 gcaaaaatta cggctcctcg ctgcatacct gcgagcaggg aaacgctccc ctcacagacg
      241 cgttgaattg tccccacgcc gcgcccctgt agagaaatat aaaaggttag gatttgccac
      301 tgaggttctt ctttcatata cttcctttta aaatcttgct aggatacagt tctcacatca
      361 catccgaaca taaacaacca ttagtgccac catggactac aaggacgatg atgacaagag
      421 cagaggcaga accagaaaca actacggctc taccatcgag ggcttgctcg acctcccaga
      481 cgacgacgac gctccagccg aagcaggtct cgttgctcca agaatgtctt tcctctccgc
      541 tggacagaga ccaagaagac tttctaccac cgctccaatc accgacgttt ctttggttga
      601 cgaattgaga ttggacggcg aggaggttga catgacccca gccgacgcct tggacgactt
      661 cgacttggag atgttgggtg acgttgagtc cccatcccca ggcatgaccc acgacccagt
      721 ttcctacggc gctttggacg ttgacgactt cgagtttgag cagatgttca ccgacgcctt
      781 gggcatcgac gacttcggtg gctatagcag aggcagaacc agaaacaact acggctctac
      841 catcgagggc ttgctcgacc tcccagacga cgacgacgct ccagccgaag caggtctcgt
      901 tgctccaaga atgtctttcc tctccgctgg acagagacca agaagacttt ctaccaccgc
      961 tccaatcacc gacgtttctt tggttgacga attgagattg gacggcgagg aggttgacat
     1021 gaccccagcc gacgccttgg acgacttcga cttggagatg ttgggtgacg ttgagtcccc
     1081 atccccaggc atgacccacg acccagtttc ctacggcgct ttggacgttg acgacttcga
     1141 gtttgagcag atgttcaccg acgccttggg catcgacgac ttcggtggct atagcagagg
     1201 cagaaccaga aacaactacg gctctaccat cgagggcttg ctcgacctcc cagacgacga
     1261 cgacgctcca gccgaagcag gtctcgttgc tccaagaatg tctttcctct ccgctggaca
     1321 gagaccaaga agactttcta ccaccgctcc aatcaccgac gtttctttgg ttgacgaatt
     1381 gagattggac ggcgaggagg ttgacatgac cccagccgac gccttggacg acttcgactt
     1441 ggagatgttg ggtgacgttg agtccccatc cccaggcatg acccacgacc cagtttccta
     1501 cggcgctttg gacgttgacg acttcgagtt tgagcagatg ttcaccgacg ccttgggcat
     1561 cgacgacttc ggtggctata gatctcccaa gaagaagcgc aaggtgatga aaaacataaa
     1621 tgccgacgac acatacagaa taattaataa aattaaagct tgtagaagca ataatgatat
     1681 taatcaatgc ttatctgata tgactaaaat ggtacattgt gaatattatt tactcgcgat
     1741 catttatcct cattctatgg ttaaatctga tatttcaatc ctagataatt accctaaaaa
     1801 atggaggcaa tattatgatg acgctaattt aataaaatat gatcctatag tagattattc
     1861 taactccaat cattcaccaa ttaattggaa tatatttgaa aacaatgctg taaataaaaa
     1921 atctccaaat gtaattaaag aagcgaaaac atcaggtctt atcactgggt ttagtttccc
     1981 tattcatacg gctaacaatg gcttcggaat gcttagtttt gcacattcag aaaaagacaa
     2041 ctatatagat agtttatttt tacatgcgtg tatgaacata ccattaattg ttccttctct
     2101 agttgataat tatcgaaaaa taaatatagc aaataataaa tcaaacaacg atttaaccaa
     2161 aagagaaaaa gaatgtttag cgtgggcatg cgaaggaaaa agctcttggg atatttcaaa
     2221 aatattaggt tgcagtgagc gtactgtcac tttccattta accaatgcgc aaatgaaact
     2281 caatacaaca aaccgctgcc aaagtatttc taaagcaatt ttaacaggag caattgattg
     2341 cccatacttt aaaaattaat aacactgata gtgctagtgt agatcacacc tgtaggatcg
     2401 tacaggttta cgcaagaaaa tggtttgtta tagtcgaata aagcatgtgc tctgtatgta
     2461 tataaaactc ttgttttctt cttttctcta aatattcttt ccttatacat taggaccttt
     2521 gcagcataaa ttactatact tctatggatc catgtctaga ttagataaaa gtaaagtgat
     2581 taacagcgca ttagagctgc ttaatgaggt cggaatcgaa ggtttaacaa cccgtaaact
     2641 cgcccagaag ctaggtgtag agcagcctac attgtattgg catgtaaaaa ataagcgggc
     2701 tttgctcgac gccttagcca ttgagatgtt agataggcac catactcact tttgcccttt
     2761 agaaggggaa agctggcaag attttttacg taataacgct aaaagtttta gatgtgcttt
     2821 actaagtcat cgcgatggag caaaagtaca tttaggtaca cggcctacag aaaaacagta
     2881 tgaaactctc gaaaatcaat tagccttttt atgccaacaa ggtttttcac tagagaatgc
     2941 attatatgca ctcagcgctg tggggcattt tactttaggt tgcgtattgg aagatcaaga
     3001 gcatcaagtc gctaaagaag aaagggaaac acctactact gatagtatgc cgccattatt
     3061 acgacaagct atcgaattat ttgatcacca aggtgcagag ccagccttct tattcggcct
     3121 tgaattgatc atatgcggat tagaaaaaca acttaaatgt gaaagtgggt ccgcgtacag
     3181 ccgcgcgcgt acgaaaaaca attacgggtc taccatcgag ggcctgctcg atctcccgga
     3241 cgacgacgcc cccgaagagg cggggctggc ggctccgcgc ctgtcctttc tccccgcggg
     3301 acacacgcgc agactgtcga cggccccccc gaccgatgtc agcctggggg acgagctcca
     3361 cttagacggc gaggacgtgg cgatggcgca tgccgacgcg ctagacgatt tcgatctgga
     3421 catgttgggg gacggggatt ccccgggtcc gggatttacc ccccacgact ccgcccccta
     3481 cggcgctctg gatatggccg acttcgagtt tgagcagatg tttaccgatg cccttggaat
     3541 tgacgagtac ggtgggtag
//