BBa_K1025003
1
BBa_K1025003
Thu-E Mutation Part
2013-09-13T11:00:00Z
2015-05-08T01:08:45Z
MutD comes from E.coli W3110 genome with two animo acid mutation[1]. GFP was conserved in our lab and its GenBank accession number is KF020493.1.
[1]Schaaper, R. M. MECHANISMS OF MUTAGENESIS IN THE ESCHERICHIA-COLI MUTATOR MUTD5 - ROLE OF DNA MISMATCH REPAIR. Proc. Natl. Acad. Sci. U. S. A.85, 8126-8130, doi:10.1073/pnas.85.21.8126 (1988)
iGEM mut part is a plasmid constructed by 2013 iGEM Tsinghua-E team which is used for genome level in vivo high-diversity library construction. In this vector, highly error-prone dnaQ mutant, mutD[1] was cloned downstream of araBAD promoter so that we can control the mutation intensity of the target genome by the concentration of araBAD promoter???s inducer, L-arabinose in a strict manner. And the GFP downstream mutD was to test whether the mutD can be translated. Actually, we measured the mutation increase induced by our mut part with the protocol described in our note form. To be brief, by measuring the reversion of rifampinresistance caused by mutation in genome, we accurately determined the mutation rate increase to be as big as four times compared with negative control (either bacteria without mutD or without arabinose input).
false
false
_1332_
0
18748
9
It's complicated
true
The genes were cloned easily by PCR and connected to plasmid backbone by in-fusion cloning.
false
Weifan Liang
annotation2341351
1
GFP
range2341351
1
1049
1765
annotation2341348
1
RBS-promoter
range2341348
1
290
304
annotation2341349
1
mutD
range2341349
1
305
1036
annotation2341350
1
RBS-GFP
range2341350
1
1037
1048
annotation2341347
1
BAD promoter
range2341347
1
1
289
annotation2341352
1
dblTerm
range2341352
1
1766
1912
BBa_K1025003_sequence
1
agaaaccaattgtccatattgcatcagacattgccgtcactgcgtcttttactggctcttctcgctaaccaaaccggtaaccccgcttattaaaagcattctgtaacaaagcgggaccaaagccatgacaaaaacgcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcttacctgacgctttttatcgcaactctctactgtttctccatacccgattaaagaggagaaaatgagcactgcaattacacgccagatcgttctcgataccgaaaccaccggtatgaaccagattggtgcgcactatgaaggccacaagatcattgagattggtgccgttgaagtggtgaaccgtcgcctgacgggcaataacttccatgtttatctcaaaccggatcggctggtggatccggaagcctttggcgtacatggtattgccgatgaattttggctcgataagccgacgtttgccgaagtagccgatgagttcatggactatatccgcggcgcggagttggtgatccataacgcagcgttcgatatcggctttatggactacgagttttcgttgcttaagcgcgatattccgaagaccaataccttctgtaaggtcaccgatagccttgcggtggcgcggaaaatgtttcctggtaagcgcaacagcctcgatgcgttatgtgcccgctacgaaattgataacagtaaacgtacgctgcacggggtattactcgatgcccagatccttgcggaagtttatctggcgatgaccggtggtcaaacgtcgatggcttttgcgatggaaggagagacacaacagcaacaaggtgaagcaacaattcagcgcattgtacgtcaggcaagtaagttacgcgttgtttttgcgacagatgacgagcttgcagcacatgaagcccgtcttgatctggtgcagaagaagggcggaagctgcctctggcgggcataaaggaggaaaaaaatgcgtaaaggcgaagagctgttcactggtgtcgtccctattctggtggaactggatggtgatgtcaacggtcataagttttccgtgcgtggcgagggtgaaggtgacgcaactaatggtaaactgacgctgaagttcatctgtactactggtaaactgccggtaccttggccgactctggtaacgacgctgacttatggtgttcagtgctttgctcgttatccggaccatatgaagcagcatgacttcttcaagtccgccatgccggaaggctatgtgcaggaacgcacgatttcctttaaggatgacggcacgtacaaaacgcgtgcggaagtgaaatttgaaggcgataccctggtaaaccgcattgagctgaaaggcattgactttaaagaagacggcaatatcctgggccataagctggaatacaattttaacagccacaatgtttacatcaccgccgataaacaaaaaaatggcattaaagcgaattttaaaattcgccacaacgtggaggatggcagcgtgcagctggctgatcactaccagcaaaacactccaatcggtgatggtcctgttctgctgccagacaatcactatctgagcacgcaaagcgttctgtctaaagatccgaacgagaaacgcgatcatatggttctgctggagttcgtaaccgcagcgggcatcacgcatggtatggatgaactgtacaaatgataaggccggccactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z