LOCUS BBa_K1025005 3696 bp DNA linear 01-Nov-2024 DEFINITION Tryptophan-sensor Part ACCESSION BBa_K1025005 VERSION BBa_K1025005.1 FEATURES Location/Qualifiers promoter 1..62 /label=tac promoter RBS 62..72 /label=RBS_tac CDS 81..155 /label=tanC misc_feature 156..362 /label=Rho binding site RBS 363..375 /label=RBS_tnaA CDS 376..3450 /label=lacZ misc_feature 3539..3696 /label=rrnB_ternimator ORIGIN 1 ttgacaatta atcatccggc tcgtataatg tgtggaattg tgagcggata acaatttcac 61 acaggaaaca gaccatggct atgaatatct tacatatatg tgtgacctca aaatggttca 121 atattgacaa caaaattgtc gatcaccgcc cttgatttgc ccttctgtag ccatcaccag 181 agccaaaccg attagattca atgtgatcta tttgtttgct atatcttaat tttgcctttt 241 gcaaaggtca tctctcgttt atttacttgt tttagtaaat gatggtgctt gcatatatat 301 ctggcgaatt aatcggtata gcagatgtaa tattcacagg gatcactgta attaaaataa 361 atgaaggatt atgtaatgac catgattacg gattcactgg ccgtcgtttt acaacgtcgt 421 gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc 481 agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg 541 aatggcgaat ggcgctttgc ctggtttccg gcaccagaag cggtgccgga aagctggctg 601 gagtgcgatc ttcctgaggc cgatactgtc gtcgtcccct caaactggca gatgcacggt 661 tacgatgcgc ccatctacac caacgtgacc tatcccatta cggtcaatcc gccgtttgtt 721 cccacggaga atccgacggg ttgttactcg ctcacattta atgttgatga aagctggcta 781 caggaaggcc agacgcgaat tatttttgat ggcgttaact cggcgtttca tctgtggtgc 841 aacgggcgct gggtcggtta cggccaggac agtcgtttgc cgtctgaatt tgacctgagc 901 gcatttttac gcgccggaga aaaccgcctc gcggtgatgg tgctgcgctg gagtgacggc 961 agttatctgg aagatcagga tatgtggcgg atgagcggca ttttccgtga cgtctcgttg 1021 ctgcataaac cgactacaca aatcagcgat ttccatgttg ccactcgctt taatgatgat 1081 ttcagccgcg ctgtactgga ggctgaagtt cagatgtgcg gcgagttgcg tgactaccta 1141 cgggtaacag tttctttatg gcagggtgaa acgcaggtcg ccagcggcac cgcgcctttc 1201 ggcggtgaaa ttatcgatga gcgtggtggt tatgccgatc gcgtcacact acgtctgaac 1261 gtcgaaaacc cgaaactgtg gagcgccgaa atcccgaatc tctatcgtgc ggtggttgaa 1321 ctgcacaccg ccgacggcac gctgattgaa gcagaagcct gcgatgtcgg tttccgcgag 1381 gtgcggattg aaaatggtct gctgctgctg aacggcaagc cgttgctgat tcgaggcgtt 1441 aaccgtcacg agcatcatcc tctgcatggt caggtcatgg atgagcagac gatggtgcag 1501 gatatcctgc tgatgaagca gaacaacttt aacgccgtgc gctgttcgca ttatccgaac 1561 catccgctgt ggtacacgct gtgcgaccgc tacggcctgt atgtggtgga tgaagccaat 1621 attgaaaccc acggcatggt gccaatgaat cgtctgaccg atgatccgcg ctggctaccg 1681 gcgatgagcg aacgcgtaac gcgaatggtg cagcgcgatc gtaatcaccc gagtgtgatc 1741 atctggtcgc tggggaatga atcaggccac ggcgctaatc acgacgcgct gtatcgctgg 1801 atcaaatctg tcgatccttc ccgcccggtg cagtatgaag gcggcggagc cgacaccacg 1861 gccaccgata ttatttgccc gatgtacgcg cgcgtggatg aagaccagcc cttcccggct 1921 gtgccgaaat ggtccatcaa aaaatggctt tcgctacctg gagagacgcg cccgctgatc 1981 ctttgcgaat acgcccacgc gatgggtaac agtcttggcg gtttcgctaa atactggcag 2041 gcgtttcgtc agtatccccg tttacagggc ggcttcgtct gggactgggt ggatcagtcg 2101 ctgattaaat atgatgaaaa cggcaacccg tggtcggctt acggcggtga ttttggcgat 2161 acgccgaacg atcgccagtt ctgtatgaac ggtctggtct ttgccgaccg cacgccgcat 2221 ccagcgctga cggaagcaaa acaccagcag cagtttttcc agttccgttt atccgggcaa 2281 accatcgaag tgaccagcga atacctgttc cgtcatagcg ataacgagct cctgcactgg 2341 atggtggcgc tggatggtaa gccgctggca agcggtgaag tgcctctgga tgtcgctcca 2401 caaggtaaac agttgattga actgcctgaa ctaccgcagc cggagagcgc cgggcaactc 2461 tggctcacag tacgcgtagt gcaaccgaac gcgaccgcat ggtcagaagc cgggcacatc 2521 agcgcctggc agcagtggcg tctggcggaa aacctcagtg tgacgctccc cgccgcgtcc 2581 cacgccatcc cgcatctgac caccagcgaa atggattttt gcatcgagct gggtaataag 2641 cgttggcaat ttaaccgcca gtcaggcttt ctttcacaga tgtggattgg cgataaaaaa 2701 caactgctga cgccgctgcg cgatcagttc acccgtgcac cgctggataa cgacattggc 2761 gtaagtgaag cgacccgcat tgaccctaac gcctgggtcg aacgctggaa ggcggcgggc 2821 cattaccagg ccgaagcagc gttgttgcag tgcacggcag atacacttgc tgatgcggtg 2881 ctgattacga ccgctcacgc gtggcagcat caggggaaaa ccttatttat cagccggaaa 2941 acctaccgga ttgatggtag tggtcaaatg gcgattaccg ttgatgttga agtggcgagc 3001 gatacaccgc atccggcgcg gattggcctg aactgccagc tggcgcaggt agcagagcgg 3061 gtaaactggc tcggattagg gccgcaagaa aactatcccg accgccttac tgccgcctgt 3121 tttgaccgct gggatctgcc attgtcagac atgtataccc cgtacgtctt cccgagcgaa 3181 aacggtctgc gctgcgggac gcgcgaattg aattatggcc cacaccagtg gcgcggcgac 3241 ttccagttca acatcagccg ctacagtcaa cagcaactga tggaaaccag ccatcgccat 3301 ctgctgcacg cggaagaagg cacatggctg aatatcgacg gtttccatat ggggattggt 3361 ggcgacgact cctggagccc gtcagtatcg gcggaattcc agctgagcgc cggtcgctac 3421 cattaccagt tggtctggtg tcaaaaataa aagcttggct gttttggcgg atgagagaag 3481 attttcagcc tgatacagat taaatcagaa cgcagaagcg gtctgataaa acagaatttg 3541 cctggcggca gtagcgcggt ggtcccacct gaccccatgc cgaactcaga agtgaaacgc 3601 cgtagcgccg atggtagtgt ggggtctccc catgcgagag tagggaactg ccaggcatca 3661 aataaaacga aaggctcagt cgaaagactg ggcctt //