LOCUS       BBa_K1025006            1823 bp    DNA     linear       26-Dec-2024
DEFINITION  Bitter Defender Part （RBS_B0030）
ACCESSION   BBa_K1025006
VERSION     BBa_K1025006.1  
FEATURES             Location/Qualifiers
     promoter        1..62
                     /label=tac promoter
     RBS             63..72
                     /label=RBS_tac
     CDS             81..155
                     /label=tnaC
     misc_feature    156..362
                     /label=Rho binding site
     RBS             363..377
                     /label=RBS_B0030
     CDS             378..1577
                     /label=tetA
     misc_feature    1666..1823
                     /label=rrnB_ternimator
ORIGIN
        1 ttgacaatta atcatccggc tcgtataatg tgtggaattg tgagcggata acaatttcac
       61 acaggaaaca gaccatggct atgaatatct tacatatatg tgtgacctca aaatggttca
      121 atattgacaa caaaattgtc gatcaccgcc cttgatttgc ccttctgtag ccatcaccag
      181 agccaaaccg attagattca atgtgatcta tttgtttgct atatcttaat tttgcctttt
      241 gcaaaggtca tctctcgttt atttacttgt tttagtaaat gatggtgctt gcatatatat
      301 ctggcgaatt aatcggtata gcagatgtaa tattcacagg gatcactgta attaaaataa
      361 atattaaaga ggagaaaatg aaacccaaca tacccctgat cgtaattctg agcactgtcg
      421 cgctcgacgc tgtcggcatc ggcctgatta tgccggtgct gccgggcctc ctgcgcgatc
      481 tggttcactc gaacgacgtc accgcccact atggcattct gctggcgctg tatgcgttgg
      541 tgcaatttgc ctgcgcacct gtgctgggcg cgctgtcgga tcgtttcggg cggcggccaa
      601 tcttgctcgt ctcgctggcc ggcgccactg tcgactacgc catcatggcg acagcgcctt
      661 tcctttgggt tctctatatc gggcggatcg tggccggcat caccggggcg actggggcgg
      721 tagccggcgc ttatattgcc gatatcactg atggcgatga gcgcgcgcgg cacttcggct
      781 tcatgagcgc ctgtttcggg ttcgggatgg tcgcgggacc tgtgctcggt gggctgatgg
      841 gcggtttctc cccccacgct ccgttcttcg ccgcggcagc cttgaacggc ctcaatttcc
      901 tgacgggctg tttccttttg ccggagtcgc acaaaggcga acgccggccg ttacgccggg
      961 aggctctcaa cccgctcgct tcgttccggt gggcccgggg catgaccgtc gtcgccgccc
     1021 tgatggcggt cttcttcatc atgcaacttg tcggacaggt gccggccgcg ctttgggtca
     1081 ttttcggcga ggatcgcttt cactgggacg cgaccacgat cggcatttcg cttgccgcat
     1141 ttggcattct gcattcactc gcccaggcaa tgatcaccgg ccctgtagcc gcccggctcg
     1201 gcgaaaggcg ggcactcatg ctcggaatga ttgccgacgg cacaggctac atcctgcttg
     1261 ccttcgcgac acggggatgg atggcgttcc cgatcatggt cctgcttgct tcgggtggca
     1321 tcggaatgcc ggcgctgcaa gcaatgttgt ccaggcaggt ggatgaggaa cgtcaggggc
     1381 agctgcaagg ctcactggcg gcgctcacca gcctgacctc gatcgtcgga cccctcctct
     1441 tcacggcgat ctatgcggct tctataacaa cgtggaacgg gtgggcatgg attgcaggcg
     1501 ctgccctcta cttgctctgc ctgccggcgc tgcgtcgcgg gctttggagc ggcgcagggc
     1561 aacgagccga tcgctgaaag cttggctgtt ttggcggatg agagaagatt ttcagcctga
     1621 tacagattaa atcagaacgc agaagcggtc tgataaaaca gaatttgcct ggcggcagta
     1681 gcgcggtggt cccacctgac cccatgccga actcagaagt gaaacgccgt agcgccgatg
     1741 gtagtgtggg gtctccccat gcgagagtag ggaactgcca ggcatcaaat aaaacgaaag
     1801 gctcagtcga aagactgggc ctt
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