LOCUS BBa_K1026000 4311 bp DNA linear 22-Dec-2024 DEFINITION Constitutively Expressed dCas9 Operon ACCESSION BBa_K1026000 VERSION BBa_K1026000.1 FEATURES Location/Qualifiers promoter 1..35 /label=BBa_J23100 misc_feature 48..51 /label=conserved RBS 44..55 /label=BBa_B0034 CDS 62..4174 /label=BBa_K1026001 stem_loop 4194..4237 /label=stem_loop misc_feature 4183..4262 /label=BBa_B0010 terminator 4183..4262 /label=BBa_B0010 stem_loop 4278..4297 /label=T7 TE misc_feature 4304..4304 /label=stop polyA_site 4298..4311 /label=polya misc_feature 4271..4311 /label=BBa_B0012 terminator 4271..4311 /label=BBa_B0012 terminator 4183..4311 /label=BBa_B0015 ORIGIN 1 ttgacggcta gctcagtcct aggtacagtg ctagctacta gagaaagagg agaaatacta 61 gatggataag aaatactcaa taggcttagc tatcggcaca aatagcgtcg gatgggcggt 121 gatcactgat gaatataagg ttccgtctaa aaagttcaag gttctgggaa atacagaccg 181 ccacagtatc aaaaaaaatc ttataggggc tcttttattt gacagtggag agacagcgga 241 agcgactcgt ctcaaacgga cagctcgtag aaggtataca cgtcggaaga atcgtatttg 301 ttatctacag gagatttttt caaatgagat ggcgaaagta gatgatagtt tctttcatcg 361 acttgaagag tcttttttgg tggaagaaga caagaagcat gaacgtcatc ctatttttgg 421 aaatatagta gatgaagttg cttatcatga gaaatatcca actatctatc atctgcgaaa 481 aaaattggta gattctactg ataaagcgga tttgcgctta atctatttgg ccttagcgca 541 tatgattaag tttcgtggtc attttttgat tgagggagat ttaaatcctg ataatagtga 601 tgtggacaaa ctatttatcc agttggtaca aacctacaat caattatttg aagaaaaccc 661 tattaacgca agtggagtag atgctaaagc gattctttct gcacgattga gtaaatcaag 721 acgattagaa aatctcattg ctcagctccc cggtgagaag aaaaatggct tatttgggaa 781 tctcattgct ttgtcattgg gtttgacccc taattttaaa tcaaattttg atttggcaga 841 agatgctaaa ttacagcttt caaaagatac ttacgatgat gatttagata atttattggc 901 gcaaattgga gatcaatatg ctgatttgtt tttggcagct aagaatttat cagatgctat 961 tttactttca gatatcctaa gagtaaatac tgaaataact aaggctcccc tatcagcttc 1021 aatgattaaa cgctacgatg aacatcatca agacttgact cttttaaaag ctttagttcg 1081 acaacaactt ccagaaaagt ataaagaaat cttttttgat caatcaaaaa acggatatgc 1141 aggttatatt gatgggggag ctagccaaga agaattttat aaatttatca aaccaatttt 1201 agaaaaaatg gatggtactg aggaattatt ggtgaaacta aatcgtgaag atttgctgcg 1261 caagcaacgg acctttgaca acggctctat tccccatcaa attcacttgg gtgagctgca 1321 tgctattttg agaagacaag aagactttta tccattttta aaagacaatc gtgagaagat 1381 tgaaaaaatc ttgacttttc gaattcctta ttatgttggt ccattggcgc gtggcaatag 1441 tcgttttgca tggatgactc ggaagtctga agaaacaatt accccatgga attttgaaga 1501 agttgtcgat aaaggtgctt cagctcaatc atttattgaa cgcatgacaa actttgataa 1561 aaatcttcca aatgaaaaag tactaccaaa acatagtttg ctttatgagt attttacggt 1621 ttataacgaa ttgacaaagg tcaaatatgt tactgaagga atgcgaaaac cagcatttct 1681 ttcaggtgaa cagaagaaag ccattgttga tttactcttc aaaacaaatc gaaaagtaac 1741 cgttaagcaa ttaaaagaag attatttcaa aaaaatagaa tgttttgata gtgttgaaat 1801 ttcaggagtt gaagatagat ttaatgcttc attaggtacc taccatgatt tgctaaaaat 1861 tattaaagat aaagattttt tggataatga agaaaatgaa gatatcttag aggatattgt 1921 tttaacattg accttatttg aagataggga gatgattgag gaaagactta aaacatatgc 1981 tcacctcttt gatgataagg tgatgaaaca gcttaaacgt cgccgttata ctggttgggg 2041 acgtttgtct cgaaaattga ttaatggtat tagggataag caatctggca aaacaatatt 2101 agattttttg aaatcagatg gttttgccaa tcgcaatttt atgcagctga tccatgatga 2161 tagtttgaca tttaaagaag acattcaaaa agcacaagtg tctggacaag gcgatagttt 2221 acatgaacat attgcaaatt tagctggtag ccctgctatt aaaaaaggta ttttacagac 2281 tgtaaaagtt gttgatgaat tggtcaaagt aatggggcgg cataagccag aaaatatcgt 2341 tattgaaatg gcacgtgaaa atcagacaac tcaaaagggc cagaaaaatt cgcgagagcg 2401 tatgaaacga atcgaagaag gtatcaaaga attaggaagt cagattctta aagagcatcc 2461 tgttgaaaat actcaattgc aaaatgaaaa gctctatctc tattatctcc aaaatggaag 2521 agacatgtat gtggaccaag aattagatat taatcgttta agtgattatg atgtcgatgc 2581 cattgttcca caaagtttcc ttaaagacga ttcaatagac aataaggtct taacgcgttc 2641 tgataaaaat cgtggtaaat cggataacgt tccaagtgaa gaagtagtca aaaagatgaa 2701 aaactattgg agacaacttc taaacgccaa gttaatcact caacgtaagt ttgataattt 2761 aacgaaagct gaacgtggag gtttgagtga acttgataaa gctggtttta tcaaacgcca 2821 attggttgaa actcgccaaa tcactaagca tgtggcacaa attttggata gtcgcatgaa 2881 tactaaatac gatgaaaatg ataaacttat tcgagaggtt aaagtgatta ccttaaaatc 2941 taaattagtt tctgacttcc gaaaagattt ccaattctat aaagtacgtg agattaacaa 3001 ttaccatcat gcccatgatg cgtatctaaa tgccgtcgtt ggaactgctt tgattaagaa 3061 atatccaaaa cttgaatcgg agtttgtcta tggtgattat aaagtttatg atgttcgtaa 3121 aatgattgct aagtctgagc aagaaatagg caaagcaacc gcaaaatatt tcttttactc 3181 taatatcatg aacttcttca aaacagaaat tacacttgca aatggagaga ttcgcaaacg 3241 ccctctaatc gaaactaatg gggaaactgg agaaattgtc tgggataaag ggcgagattt 3301 tgccacagtg cgcaaagtat tgtccatgcc ccaagtcaat attgtcaaga aaacagaagt 3361 acagacaggc ggattctcca aggagtcaat tttaccaaaa agaaattcgg acaagcttat 3421 tgctcgtaaa aaagactggg atccaaaaaa atatggtggt tttgatagtc caacggtagc 3481 ttattcagtc ctagtggttg ctaaggtgga aaaagggaaa tcgaagaagt taaaatccgt 3541 taaagagtta ctagggatca caattatgga aagaagttcc tttgaaaaaa atccgattga 3601 ctttttagaa gctaaaggat ataaggaagt taaaaaagac ttaatcatta aactacctaa 3661 atatagtctt tttgagttag aaaacggtcg taaacggatg ctggctagtg ccggagaatt 3721 acaaaaagga aatgagctgg ctctgccaag caaatatgtg aattttttat atttagctag 3781 tcattatgaa aagttgaagg gtagtccaga agataacgaa caaaaacaat tgtttgtgga 3841 gcagcataag cattatttag atgagattat tgagcaaatc agtgaatttt ctaagcgtgt 3901 tattttagca gatgccaatt tagataaagt tcttagtgca tataacaaac atagagacaa 3961 accaatacgt gaacaagcag aaaatattat tcatttattt acgttgacga atcttggagc 4021 tcccgctgct tttaaatatt ttgatacaac aattgatcgt aaacgatata cgtctacaaa 4081 agaagtttta gatgccactc ttatccatca atccatcact ggtctttatg aaacacgcat 4141 tgatttgagt cagctaggag gtgactgagt cgactactag agccaggcat caaataaaac 4201 gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc 4261 tctactagag tcacactggc tcaccttcgg gtgggccttt ctgcgtttat a //