LOCUS       BBa_K1026004             339 bp    DNA     linear       08-Dec-2025
DEFINITION  A gRNA targeting mRFP, negatively regulated by Red Light Sensor
ACCESSION   BBa_K1026004
VERSION     BBa_K1026004.1  
FEATURES             Location/Qualifiers
     misc_feature    13..30
                     /label=C1 OmpR
     misc_feature    34..51
                     /label=C2 OmpR
     misc_feature    54..71
                     /label=C3 OmpR
     promoter        75..80
                     /label=-35
     promoter        98..103
                     /label=-10
     promoter        1..108
                     /label=BBa_R0082
     CDS             109..210
                     /label=BBa_K1026003
     stem_loop       222..265
                     /label=stem_loop
     misc_feature    211..290
                     /label=BBa_B0010
     terminator      211..290
                     /label=BBa_B0010
     stem_loop       306..325
                     /label=T7 TE
     misc_feature    332..332
                     /label=stop
     polyA_site      326..339
                     /label=polya
     misc_feature    299..339
                     /label=BBa_B0012
     terminator      299..339
                     /label=BBa_B0012
     terminator      211..339
                     /label=BBa_B0015
ORIGIN
        1 tcccttgcat ttacattttg aaacatctat agcgataaat gaaacatctt aaaagtttta
       61 gtatcatatt cgtgttggat tattctgcat ttttggggag aatggactaa ctttcagttt
      121 agcggtctgt tttagagcta gaaatagcaa gttaaaataa ggctagtccg ttatcaactt
      181 gaaaaagtgg caccgagtcg gtgctttttt ccaggcatca aataaaacga aaggctcagt
      241 cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc tactagagtc
      301 acactggctc accttcgggt gggcctttct gcgtttata
//