LOCUS       BBa_K1026010             497 bp    DNA     linear       22-Dec-2024
DEFINITION  A gRNA targeting mRFP, negatively regulated by Blue Light Sensor
ACCESSION   BBa_K1026010
VERSION     BBa_K1026010.1  
FEATURES             Location/Qualifiers
     promoter        1..250
                     /label=FixK2 promoter
     promoter        1..250
                     /label=BBa_K592006
     CDS             259..360
                     /label=BBa_K1026003
     stem_loop       380..423
                     /label=stem_loop
     misc_feature    369..448
                     /label=BBa_B0010
     terminator      369..448
                     /label=BBa_B0010
     stem_loop       464..483
                     /label=T7 TE
     misc_feature    490..490
                     /label=stop
     polyA_site      484..497
                     /label=polya
     misc_feature    457..497
                     /label=BBa_B0012
     terminator      457..497
                     /label=BBa_B0012
     terminator      369..497
                     /label=BBa_B0015
ORIGIN
        1 gccggagccg attatccgca cccgtccttg gtcttggaca cactcgctcg cgatccgaag
       61 ccgcctttca aagatactcc gcagtgaaac gcatctttta agcgcgactt gtcgccttcg
      121 cgcagcagaa cactcgtatg gcactcaatc catgatcgct tcgttcgctc cgacgcgcgt
      181 tgcggggccc ccctcgaccg gatgacaaca cattgcgtca cctcaactgc tgcgcgcgca
      241 ctgcgtcaca tactagagaa ctttcagttt agcggtctgt tttagagcta gaaatagcaa
      301 gttaaaataa ggctagtccg ttatcaactt gaaaaagtgg caccgagtcg gtgctttttt
      361 tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt
      421 tatctgttgt ttgtcggtga acgctctcta ctagagtcac actggctcac cttcgggtgg
      481 gcctttctgc gtttata
//