LOCUS       BBa_K1026014             973 bp    DNA     linear       26-Dec-2024
DEFINITION  Constitutively Expressed pcyA Operon
ACCESSION   BBa_K1026014
VERSION     BBa_K1026014.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23100
     misc_feature    48..51
                     /label=conserved
     RBS             44..55
                     /label=BBa_B0034
     CDS             62..811
                     /label=PcyA
     misc_feature    812..836
                     /label=Help:Barcodes
     CDS             62..836
                     /label=BBa_I15009
     stem_loop       856..899
                     /label=stem_loop
     misc_feature    845..924
                     /label=BBa_B0010
     terminator      845..924
                     /label=BBa_B0010
     stem_loop       940..959
                     /label=T7 TE
     misc_feature    966..966
                     /label=stop
     polyA_site      960..973
                     /label=polya
     misc_feature    933..973
                     /label=BBa_B0012
     terminator      933..973
                     /label=BBa_B0012
     terminator      845..973
                     /label=BBa_B0015
ORIGIN
        1 ttgacggcta gctcagtcct aggtacagtg ctagctacta gagaaagagg agaaatacta
       61 gatggccgtc actgatttaa gtttgaccaa ttcttccctg atgcctacgt tgaacccgat
      121 gattcaacag ttggccctgg cgatcgccgc tagttggcaa agtttacccc tcaagcccta
      181 tcaattgccg gaggatttgg gctacgtaga aggccgcctg gaaggggaaa agttagtgat
      241 tgaaaatcgg tgctaccaaa cgccccagtt tcgcaaaatg catttggagt tggccaaggt
      301 gggcaaaggg ttggatattc tccactgtgt aatgtttcct gagcctttat acggtctacc
      361 tttgtttggc tgtgacattg tggccggccc cggtggagta agtgcggcta ttgcggatct
      421 atcccccacc caaagcgatc gccaattgcc cgcagcgtac caaaaatcat tggcagagct
      481 aggccagcca gaatttgagc aacaacggga attgcccccc tggggagaaa tattttctga
      541 atattgttta ttcatccgtc ccagcaatgt cactgaagaa gaaagatttg tacaaagggt
      601 agtggacttt ttgcaaattc attgtcacca atccatcgtt gccgaaccct tgtctgaagc
      661 tcaaactttg gagcaccgtc aggggcaaat tcattactgc caacaacaac agaaaaatga
      721 taaaacccgt cgggtactgg aaaaagcttt tggggaagct tgggcggaac ggtatatgag
      781 ccaagtctta tttgatgtta tccaataata actctgatag tgctagtgta gatctctact
      841 agagccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc
      901 tgttgtttgt cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct
      961 ttctgcgttt ata
//