BBa_K1028001 1 BBa_K1028001 INP.Si4 Silica Binding Complex 2013-09-17T11:00:00Z 2015-05-08T01:08:46Z This part is based on the Edinburgh 2011 iGEM team's Ice Nucleation Protein part BBa_K523008 with the Si4 binding peptide part BBa_K1028000 submitted by Leeds 2013 iGEM team. Fusion of the silica binding peptide Si4, part BBa_K1028000, to Ice Nucleation Protein (INP) to achieve presentation and expression of the binding domain on the outer membrane. false false _1335_ 0 15658 9 It's complicated false The Si4 part was added at the C-terminus of INP using PCR assembly methods. false Joseph Beton component2352383 1 BBa_K523008 component2352387 1 BBa_K1028000 annotation2352387 1 BBa_K1028000 range2352387 1 931 981 annotation2352383 1 BBa_K523008 range2352383 1 1 930 BBa_K523008 1 BBa_K523008 INP for BioSandwich 2011-08-03T11:00:00Z 2015-05-08T01:12:33Z From <partinfo>BBa_K265008</partinfo>. Released HQ 2013 This is a modified version of <partinfo>BBa_K265008</partinfo> for use with the BioSandwich protocol of Edinburgh 2011. false false _688_ 0 8799 9 In stock false N/A. false Mun Ching Lee, Sylvia Ispasanie annotation2124325 1 BglII range2124325 1 1 4 annotation2124327 1 GG range2124327 1 929 930 annotation2124328 1 INPNC range2124328 1 5 928 annotation2124326 1 ATG range2124326 1 5 5 BBa_K1028000 1 Si4 Silica binding peptide 2013-08-29T11:00:00Z 2015-05-08T01:08:46Z This sequence is taken from the "Site-Speci&#64257;c Labeling of Surface Proteins on Living Cells Using Genetically Encoded Peptides that Bind Fluorescent Nanoparticle Probes" paper published by Rocco et al. This is a short peptide of 15 amino acids designed to bind with high affinity to silica and silica-like particles, designed as a tag to be attached to the C-terminus of polypeptides. This part was originally designed to be conjugated to the C-terminal of Ice Nucleation Protein allowing extracellular expression of the binding region. false false _1335_ 0 15658 9 In stock false The sequence was codon optomised for E.Coli expression. false Oleg Karpov, Joseph Beton annotation2333673 1 stop codon range2333673 1 49 49 annotation2333674 1 Coding region range2333674 1 1 45 annotation2333672 1 stop codon range2333672 1 46 46 BBa_K523008_sequence 1 atctatgaccctggataaagcgctggtgctgcgcacttgtgccaataatatggccgaccattgtggcctgatctggcctgcttctggtacagttgaaagccgctattggcagtctacacgtcgtcacgaaaacgggctggttgggctgctgtggggtgccggaacatccgcctttctgagcgttcatgccgatgctcgttggattgtttgtgaggttgctgttgccgacatcattagcctggaagaaccgggtatggtgaaatttcctcgtgctgaagtggtccatgttggtgatcgtattagcgcctcccatttcatttcagctcgtcaggctgatccagcaagcacatcaacaagcacctctaccagtacactgacaccaatgccaaccgctatccctactcctatgccggctgtagcctctgttactctgcctgtggccgaacaagctcgccacgaagtctttgacgttgcctctgtgtctgctgctgctgctcctgttaacactctgcctgttaccacgcctcaaaatctgcaaacggcgacctatggctcaacactgagtggtgataaccattctcgtctgattgccgggtatggctctaacgaaaccgccggaaaccattctgatctgatcggtggtcacgattgtacgctgatggcaggtgatcaatctcgtctgaccgctggcaaaaattccgttctgaccgctggggcacgttctaaactgatcgggagcgaaggctctactctgtccgccggagaagattctaccctgatctttcgtctgtgggatggtaaacgttatcgtcagctggtcgctcgtacaggtgaaaatggtgtggaagccgacattccgtattatgtgaatgaggacgatgatattgtggacaaaccggacgaagatgatgattggatcgaagtgaaagg BBa_K1028001_sequence 1 atctatgaccctggataaagcgctggtgctgcgcacttgtgccaataatatggccgaccattgtggcctgatctggcctgcttctggtacagttgaaagccgctattggcagtctacacgtcgtcacgaaaacgggctggttgggctgctgtggggtgccggaacatccgcctttctgagcgttcatgccgatgctcgttggattgtttgtgaggttgctgttgccgacatcattagcctggaagaaccgggtatggtgaaatttcctcgtgctgaagtggtccatgttggtgatcgtattagcgcctcccatttcatttcagctcgtcaggctgatccagcaagcacatcaacaagcacctctaccagtacactgacaccaatgccaaccgctatccctactcctatgccggctgtagcctctgttactctgcctgtggccgaacaagctcgccacgaagtctttgacgttgcctctgtgtctgctgctgctgctcctgttaacactctgcctgttaccacgcctcaaaatctgcaaacggcgacctatggctcaacactgagtggtgataaccattctcgtctgattgccgggtatggctctaacgaaaccgccggaaaccattctgatctgatcggtggtcacgattgtacgctgatggcaggtgatcaatctcgtctgaccgctggcaaaaattccgttctgaccgctggggcacgttctaaactgatcgggagcgaaggctctactctgtccgccggagaagattctaccctgatctttcgtctgtgggatggtaaacgttatcgtcagctggtcgctcgtacaggtgaaaatggtgtggaagccgacattccgtattatgtgaatgaggacgatgatattgtggacaaaccggacgaagatgatgattggatcgaagtgaaaggggcggtgggactcatcaccatcgccctcatccacatccgtcgatgtaataa BBa_K1028000_sequence 1 ggcggtgggactcatcaccatcgccctcatccacatccgtcgatgtaataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z