LOCUS       BBa_K1028002             851 bp    DNA     linear       22-Dec-2024
DEFINITION  A cell membrane stress reporter gene
ACCESSION   BBa_K1028002
VERSION     BBa_K1028002.1  
FEATURES             Location/Qualifiers
     misc_feature    1..15
                     /label=CpxR-P Binding site 
     promoter        1..55
                     /label=BBa_K135000
     promoter        1..55
                     /label=BBa_K135000
     RBS             64..78
                     /label=RBS-1\Strong
     RBS             71..75
                     /label=RBS
     misc_feature    64..78
                     /label=BBa_B0030
     RBS             64..78
                     /label=BBa_B0030
     CDS             85..804
                     /label=GFP protein
     CDS             85..804
                     /label=BBa_E0040
     misc_feature    813..851
                     /label=B1006
     stem_loop       822..843
                     /label=modified thr terminator
     polyA_site      844..851
                     /label=PolyA
     polyA_site      813..821
                     /label=PolyA
     terminator      813..851
                     /label=BBa_B1006
     misc_feature    64..851
                     /label=BBa_K081012
ORIGIN
        1 gtaaaacaac gtaaagtcat ggattagcga cgtctgatga cgtaatttct gcctctacta
       61 gagattaaag aggagaaata ctagatgcgt aaaggagaag aacttttcac tggagttgtc
      121 ccaattcttg ttgaattaga tggtgatgtt aatgggcaca aattttctgt cagtggagag
      181 ggtgaaggtg atgcaacata cggaaaactt acccttaaat ttatttgcac tactggaaaa
      241 ctacctgttc catggccaac acttgtcact actttcggtt atggtgttca atgctttgcg
      301 agatacccag atcatatgaa acagcatgac tttttcaaga gtgccatgcc cgaaggttat
      361 gtacaggaaa gaactatatt tttcaaagat gacgggaact acaagacacg tgctgaagtc
      421 aagtttgaag gtgataccct tgttaataga atcgagttaa aaggtattga ttttaaagaa
      481 gatggaaaca ttcttggaca caaattggaa tacaactata actcacacaa tgtatacatc
      541 atggcagaca aacaaaagaa tggaatcaaa gttaacttca aaattagaca caacattgaa
      601 gatggaagcg ttcaactagc agaccattat caacaaaata ctccaattgg cgatggccct
      661 gtccttttac cagacaacca ttacctgtcc acacaatctg ccctttcgaa agatcccaac
      721 gaaaagagag accacatggt ccttcttgag tttgtaacag ctgctgggat tacacatggc
      781 atggatgaac tatacaaata ataatactag agaaaaaaaa accccgcccc tgacagggcg
      841 gggttttttt t
//