LOCUS       BBa_K1028003            2040 bp    DNA     linear       10-Dec-2025
DEFINITION  Silica Detection System
ACCESSION   BBa_K1028003
VERSION     BBa_K1028003.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23119
     misc_feature    48..51
                     /label=conserved
     RBS             44..55
                     /label=BBa_B0034
     misc_feature    64..67
                     /label=BglII
     misc_feature    68..68
                     /label=ATG
     misc_feature    992..993
                     /label=GG
     CDS             68..991
                     /label=INPNC
     CDS             64..993
                     /label=BBa_K523008
     misc_feature    1039..1039
                     /label=stop codon
     misc_feature    1042..1042
                     /label=stop codon
     misc_feature    994..1038
                     /label=Coding region
     misc_feature    994..1044
                     /label=BBa_K1028000
     misc_feature    64..1044
                     /label=BBa_K1028001
     stem_loop       1064..1107
                     /label=stem_loop
     misc_feature    1053..1132
                     /label=BBa_B0010
     terminator      1053..1132
                     /label=BBa_B0010
     stem_loop       1148..1167
                     /label=T7 TE
     misc_feature    1174..1174
                     /label=stop
     polyA_site      1168..1181
                     /label=polya
     misc_feature    1141..1181
                     /label=BBa_B0012
     terminator      1141..1181
                     /label=BBa_B0012
     terminator      1053..1181
                     /label=BBa_B0015
     misc_feature    1190..1204
                     /label=CpxR-P Binding site 
     promoter        1190..1244
                     /label=BBa_K135000
     promoter        1190..1244
                     /label=BBa_K135000
     RBS             1253..1267
                     /label=RBS-1\Strong
     RBS             1260..1264
                     /label=RBS
     misc_feature    1253..1267
                     /label=BBa_B0030
     RBS             1253..1267
                     /label=BBa_B0030
     CDS             1274..1993
                     /label=GFP protein
     CDS             1274..1993
                     /label=BBa_E0040
     misc_feature    2002..2040
                     /label=B1006
     stem_loop       2011..2032
                     /label=modified thr terminator
     polyA_site      2033..2040
                     /label=PolyA
     polyA_site      2002..2010
                     /label=PolyA
     terminator      2002..2040
                     /label=BBa_B1006
     misc_feature    1253..2040
                     /label=BBa_K081012
     misc_feature    1190..2040
                     /label=BBa_K1028002
ORIGIN
        1 ttgacagcta gctcagtcct aggtataatg ctagctacta gagaaagagg agaaatacta
       61 gagatctatg accctggata aagcgctggt gctgcgcact tgtgccaata atatggccga
      121 ccattgtggc ctgatctggc ctgcttctgg tacagttgaa agccgctatt ggcagtctac
      181 acgtcgtcac gaaaacgggc tggttgggct gctgtggggt gccggaacat ccgcctttct
      241 gagcgttcat gccgatgctc gttggattgt ttgtgaggtt gctgttgccg acatcattag
      301 cctggaagaa ccgggtatgg tgaaatttcc tcgtgctgaa gtggtccatg ttggtgatcg
      361 tattagcgcc tcccatttca tttcagctcg tcaggctgat ccagcaagca catcaacaag
      421 cacctctacc agtacactga caccaatgcc aaccgctatc cctactccta tgccggctgt
      481 agcctctgtt actctgcctg tggccgaaca agctcgccac gaagtctttg acgttgcctc
      541 tgtgtctgct gctgctgctc ctgttaacac tctgcctgtt accacgcctc aaaatctgca
      601 aacggcgacc tatggctcaa cactgagtgg tgataaccat tctcgtctga ttgccgggta
      661 tggctctaac gaaaccgccg gaaaccattc tgatctgatc ggtggtcacg attgtacgct
      721 gatggcaggt gatcaatctc gtctgaccgc tggcaaaaat tccgttctga ccgctggggc
      781 acgttctaaa ctgatcggga gcgaaggctc tactctgtcc gccggagaag attctaccct
      841 gatctttcgt ctgtgggatg gtaaacgtta tcgtcagctg gtcgctcgta caggtgaaaa
      901 tggtgtggaa gccgacattc cgtattatgt gaatgaggac gatgatattg tggacaaacc
      961 ggacgaagat gatgattgga tcgaagtgaa aggggcggtg ggactcatca ccatcgccct
     1021 catccacatc cgtcgatgta ataatactag agccaggcat caaataaaac gaaaggctca
     1081 gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tctactagag
     1141 tcacactggc tcaccttcgg gtgggccttt ctgcgtttat atactagagg taaaacaacg
     1201 taaagtcatg gattagcgac gtctgatgac gtaatttctg cctctactag agattaaaga
     1261 ggagaaatac tagatgcgta aaggagaaga acttttcact ggagttgtcc caattcttgt
     1321 tgaattagat ggtgatgtta atgggcacaa attttctgtc agtggagagg gtgaaggtga
     1381 tgcaacatac ggaaaactta cccttaaatt tatttgcact actggaaaac tacctgttcc
     1441 atggccaaca cttgtcacta ctttcggtta tggtgttcaa tgctttgcga gatacccaga
     1501 tcatatgaaa cagcatgact ttttcaagag tgccatgccc gaaggttatg tacaggaaag
     1561 aactatattt ttcaaagatg acgggaacta caagacacgt gctgaagtca agtttgaagg
     1621 tgataccctt gttaatagaa tcgagttaaa aggtattgat tttaaagaag atggaaacat
     1681 tcttggacac aaattggaat acaactataa ctcacacaat gtatacatca tggcagacaa
     1741 acaaaagaat ggaatcaaag ttaacttcaa aattagacac aacattgaag atggaagcgt
     1801 tcaactagca gaccattatc aacaaaatac tccaattggc gatggccctg tccttttacc
     1861 agacaaccat tacctgtcca cacaatctgc cctttcgaaa gatcccaacg aaaagagaga
     1921 ccacatggtc cttcttgagt ttgtaacagc tgctgggatt acacatggca tggatgaact
     1981 atacaaataa taatactaga gaaaaaaaaa ccccgcccct gacagggcgg ggtttttttt
//