BBa_K1028003
type: ComponentDefinition
persistent identity: BBa K1028003
display id: BBa_K1028003
version: 1
was generated by: iGEM to SBOL conversion
description: Silica Detection System
created: 2013-09-23T11:00:00Z
modified: 2015-05-08T01:08:46Z
mutable provenance: The DNA for this part was taken from the registry
owned by: james
owned by: myers
mutable description: A fusion of the INP.Si4 complex with the hydrophobic cell stress reporter to create a system capable of detecting silica particles in solution
discontinued: false
dominant: false
experience: experience/None
group u list: _1335_
m user id: 0
owner id: 15658
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: The biobricks were digested and ligated together using the RFC10 restriction sites
star: false
creator: Joseph Beton
type: DnaRegion
role: SO:0000110
role: partType/Other
component: BBa_K1028001
component: BBa_B0034
component: BBa_K1028002
component: BBa_B0015
component: BBa_J23119
sequence annotation: BBa_J23119
sequence annotation: BBa_B0034
sequence annotation: BBa_K1028001
sequence annotation: BBa_B0015
sequence annotation: BBa_K1028002
sequence: https://synbiohub.org/public/igem/BBa_K1028003_sequence/1

BBa_K1028001
type: Component
persistent identity: component2367722
display id: component2367722
version: 1
top level: BBa_K1028003
definition: BBa_K1028001
access: public

BBa_B0034
type: Component
persistent identity: component2367712
display id: component2367712
version: 1
top level: BBa_K1028003
definition: BBa_B0034
access: public

BBa_K1028002
type: Component
persistent identity: component2367744
display id: component2367744
version: 1
top level: BBa_K1028003
definition: BBa_K1028002
access: public

BBa_B0015
type: Component
persistent identity: component2367729
display id: component2367729
version: 1
top level: BBa_K1028003
definition: BBa_B0015
access: public

BBa_J23119
type: Component
persistent identity: component2367710
display id: component2367710
version: 1
top level: BBa_K1028003
definition: BBa_J23119
access: public

BBa_J23119
type: SequenceAnnotation
persistent identity: annotation2367710
display id: annotation2367710
version: 1
top level: BBa_K1028003
location: https://synbiohub.org/public/igem/BBa_K1028003/annotation2367710/range2367710/1
component: BBa_J23119

https://synbiohub.org/public/igem/BBa_K1028003/annotation2367710/range2367710/1
type: Range
persistent identity: range2367710
display id: range2367710
version: 1
top level: BBa_K1028003
direction: direction/reverse
start: 1
end: 35
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2367712
display id: annotation2367712
version: 1
top level: BBa_K1028003
location: https://synbiohub.org/public/igem/BBa_K1028003/annotation2367712/range2367712/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K1028003/annotation2367712/range2367712/1
type: Range
persistent identity: range2367712
display id: range2367712
version: 1
top level: BBa_K1028003
direction: direction/reverse
start: 44
end: 55
orientation: inline

BBa_K1028001
type: SequenceAnnotation
persistent identity: annotation2367722
display id: annotation2367722
version: 1
top level: BBa_K1028003
location: https://synbiohub.org/public/igem/BBa_K1028003/annotation2367722/range2367722/1
component: BBa_K1028001

https://synbiohub.org/public/igem/BBa_K1028003/annotation2367722/range2367722/1
type: Range
persistent identity: range2367722
display id: range2367722
version: 1
top level: BBa_K1028003
direction: direction/reverse
start: 64
end: 1044
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2367729
display id: annotation2367729
version: 1
top level: BBa_K1028003
location: https://synbiohub.org/public/igem/BBa_K1028003/annotation2367729/range2367729/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K1028003/annotation2367729/range2367729/1
type: Range
persistent identity: range2367729
display id: range2367729
version: 1
top level: BBa_K1028003
direction: direction/reverse
start: 1053
end: 1181
orientation: inline

BBa_K1028002
type: SequenceAnnotation
persistent identity: annotation2367744
display id: annotation2367744
version: 1
top level: BBa_K1028003
location: https://synbiohub.org/public/igem/BBa_K1028003/annotation2367744/range2367744/1
component: BBa_K1028002

https://synbiohub.org/public/igem/BBa_K1028003/annotation2367744/range2367744/1
type: Range
persistent identity: range2367744
display id: range2367744
version: 1
top level: BBa_K1028003
direction: direction/reverse
start: 1190
end: 2040
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

BBa_B0015
type: ComponentDefinition
persistent identity: BBa B0015
display id: BBa_B0015
version: 1
was generated by: iGEM to SBOL conversion
description: double terminator (B0010-B0012)
created: 2003-07-16T11:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Double terminator consisting of BBa_B0010 and BBa_B0012
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Reshma Shetty
type: DnaRegion
role: partType/Terminator
role: SO:0000141
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_B0015_sequence/1

BBa_B0012
type: Component
persistent identity: component1916612
display id: component1916612
version: 1
top level: BBa_B0015
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1916610
display id: component1916610
version: 1
top level: BBa_B0015
definition: BBa_B0010
access: public

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1916610
display id: annotation1916610
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
type: Range
persistent identity: range1916610
display id: range1916610
version: 1
top level: BBa_B0015
direction: direction/forward
start: 1
end: 80
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1916612
display id: annotation1916612
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
type: Range
persistent identity: range1916612
display id: range1916612
version: 1
top level: BBa_B0015
direction: direction/forward
start: 89
end: 129
orientation: inline

BBa_J23119
type: ComponentDefinition
persistent identity: BBa J23119
display id: BBa_J23119
version: 1
was generated by: iGEM to SBOL conversion
description: constitutive promoter family member
created: 2006-08-23T11:00:00Z
modified: 2015-08-31T04:08:40Z
mutable provenance: Overlap extension of synthetic oligonucleotides
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Later
discontinued: false
dominant: true
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: false
mutable notes: N/A
star: true
creator: John Anderson
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence: https://synbiohub.org/public/igem/BBa_J23119_sequence/1

BBa_K1028001
type: ComponentDefinition
persistent identity: BBa K1028001
display id: BBa_K1028001
version: 1
was generated by: iGEM to SBOL conversion
description: INP.Si4 Silica Binding Complex
created: 2013-09-17T11:00:00Z
modified: 2015-05-08T01:08:46Z
mutable provenance: This part is based on the Edinburgh 2011 iGEM team's Ice Nucleation Protein part BBa_K523008 with the Si4 binding peptide part BBa_K1028000 submitted by Leeds 2013 iGEM team.
owned by: james
owned by: myers
mutable description: Fusion of the silica binding peptide Si4, part BBa_K1028000, to Ice Nucleation Protein (INP) to achieve presentation and expression of the binding domain on the outer membrane.
discontinued: false
dominant: false
group u list: _1335_
m user id: 0
owner id: 15658
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: The Si4 part was added at the C-terminus of INP using PCR assembly methods.
star: false
creator: Joseph Beton
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_K1028000
component: BBa_K523008
sequence annotation: BBa_K523008
sequence annotation: BBa_K1028000
sequence: https://synbiohub.org/public/igem/BBa_K1028001_sequence/1

BBa_K1028000
type: Component
persistent identity: component2352387
display id: component2352387
version: 1
top level: BBa_K1028001
definition: Si4
access: public

BBa_K523008
type: Component
persistent identity: component2352383
display id: component2352383
version: 1
top level: BBa_K1028001
definition: BBa_K523008
access: public

BBa_K523008
type: SequenceAnnotation
persistent identity: annotation2352383
display id: annotation2352383
version: 1
top level: BBa_K1028001
location: https://synbiohub.org/public/igem/BBa_K1028001/annotation2352383/range2352383/1
component: BBa_K523008

https://synbiohub.org/public/igem/BBa_K1028001/annotation2352383/range2352383/1
type: Range
persistent identity: range2352383
display id: range2352383
version: 1
top level: BBa_K1028001
direction: direction/reverse
start: 1
end: 930
orientation: inline

BBa_K1028000
type: SequenceAnnotation
persistent identity: annotation2352387
display id: annotation2352387
version: 1
top level: BBa_K1028001
location: https://synbiohub.org/public/igem/BBa_K1028001/annotation2352387/range2352387/1
component: BBa_K1028000

https://synbiohub.org/public/igem/BBa_K1028001/annotation2352387/range2352387/1
type: Range
persistent identity: range2352387
display id: range2352387
version: 1
top level: BBa_K1028001
direction: direction/reverse
start: 931
end: 981
orientation: inline

BBa_K1028002
type: ComponentDefinition
persistent identity: BBa K1028002
display id: BBa_K1028002
version: 1
was generated by: iGEM to SBOL conversion
description: A cell membrane stress reporter gene
created: 2013-09-18T11:00:00Z
modified: 2015-05-08T01:08:46Z
mutable provenance: The parts for this were aquired from the iGEM 2013 delivery plates.
owned by: james
owned by: myers
mutable description: The CpxR promoter is activated via adhesion of the cellular membrane to hydrophobic surfaces, with GFP downstream of the promoter the gene functions to produce a visible and quantitive signal upon surface binding.
discontinued: false
dominant: false
group u list: _1335_
m user id: 0
owner id: 15658
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: The parts were assembled using PCR assembly to avoid introducing a scar site between the promoter and the ribosome binding site included in part BBa_K081012 and in assembled parallel
star: false
creator: Joseph Beton
type: DnaRegion
role: SO:0000804
role: partType/Reporter
component: BBa_K135000
component: BBa_K081012
sequence annotation: BBa_K135000
sequence annotation: BBa_K081012
sequence: https://synbiohub.org/public/igem/BBa_K1028002_sequence/1

BBa_K135000
type: Component
persistent identity: component2355476
display id: component2355476
version: 1
top level: BBa_K1028002
definition: BBa_K135000
access: public

BBa_K081012
type: Component
persistent identity: component2355487
display id: component2355487
version: 1
top level: BBa_K1028002
definition: BBa_K081012
access: public

BBa_K135000
type: SequenceAnnotation
persistent identity: annotation2355476
display id: annotation2355476
version: 1
top level: BBa_K1028002
location: https://synbiohub.org/public/igem/BBa_K1028002/annotation2355476/range2355476/1
component: BBa_K135000

https://synbiohub.org/public/igem/BBa_K1028002/annotation2355476/range2355476/1
type: Range
persistent identity: range2355476
display id: range2355476
version: 1
top level: BBa_K1028002
direction: direction/reverse
start: 1
end: 55
orientation: inline

BBa_K081012
type: SequenceAnnotation
persistent identity: annotation2355487
display id: annotation2355487
version: 1
top level: BBa_K1028002
location: https://synbiohub.org/public/igem/BBa_K1028002/annotation2355487/range2355487/1
component: BBa_K081012

https://synbiohub.org/public/igem/BBa_K1028002/annotation2355487/range2355487/1
type: Range
persistent identity: range2355487
display id: range2355487
version: 1
top level: BBa_K1028002
direction: direction/reverse
start: 64
end: 851
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

BBa_K081012
type: ComponentDefinition
persistent identity: BBa K081012
display id: BBa_K081012
version: 1
was generated by: iGEM to SBOL conversion
description: GFP protein generator - PoPS->GFP
created: 2008-10-17T11:00:00Z
modified: 2015-05-08T01:08:35Z
mutable provenance: RBS: GFP: T:
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: PoPS in -> GFP out Strong RBS (efficiency=0.6).
discontinued: false
dominant: false
experience: experience/Works
group u list: _227_
m user id: 0
owner id: 2583
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable notes: We used BioBrick Standard Assembly.
star: true
creator: Lorenzo Pasotti, Paolo Magni
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_E0040
component: BBa_B0030
component: BBa_B1006
sequence annotation: BBa_B0030
sequence annotation: BBa_E0040
sequence annotation: BBa_B1006
sequence: https://synbiohub.org/public/igem/BBa_K081012_sequence/1

BBa_E0040
type: Component
persistent identity: component1981957
display id: component1981957
version: 1
top level: BBa_K081012
definition: GFP
access: public

BBa_B0030
type: Component
persistent identity: component1981954
display id: component1981954
version: 1
top level: BBa_K081012
definition: BBa_B0030
access: public

BBa_B1006
type: Component
persistent identity: component1981962
display id: component1981962
version: 1
top level: BBa_K081012
definition: BBa_B1006
access: public

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation1981954
display id: annotation1981954
version: 1
top level: BBa_K081012
location: https://synbiohub.org/public/igem/BBa_K081012/annotation1981954/range1981954/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_K081012/annotation1981954/range1981954/1
type: Range
persistent identity: range1981954
display id: range1981954
version: 1
top level: BBa_K081012
direction: direction/forward
start: 1
end: 15
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation1981957
display id: annotation1981957
version: 1
top level: BBa_K081012
location: https://synbiohub.org/public/igem/BBa_K081012/annotation1981957/range1981957/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_K081012/annotation1981957/range1981957/1
type: Range
persistent identity: range1981957
display id: range1981957
version: 1
top level: BBa_K081012
direction: direction/reverse
start: 22
end: 741
orientation: inline

BBa_B1006
type: SequenceAnnotation
persistent identity: annotation1981962
display id: annotation1981962
version: 1
top level: BBa_K081012
location: https://synbiohub.org/public/igem/BBa_K081012/annotation1981962/range1981962/1
component: BBa_B1006

https://synbiohub.org/public/igem/BBa_K081012/annotation1981962/range1981962/1
type: Range
persistent identity: range1981962
display id: range1981962
version: 1
top level: BBa_K081012
direction: direction/reverse
start: 750
end: 788
orientation: inline

BBa_K135000
type: ComponentDefinition
persistent identity: BBa K135000
display id: BBa_K135000
version: 1
was generated by: iGEM to SBOL conversion
description: pCpxR (CpxR responsive promoter)
created: 2008-10-19T11:00:00Z
modified: 2015-05-08T01:10:01Z
mutable provenance: This part comes from the genome of E.coli TR235, as used in the PNAS paper of Otto and Silhavy mentioned previously. TR235 is K-12 MC4100 containing a pCpxR-LacZ fusion inserted at the (Lambda)att site, plus some other modifications (see Otto and Silhavy). The part is a portion of the CpxR responsive region used in these LacZ fusions.
owned by: james
owned by: myers
mutable description: This is suggested to be an adhesion responsive promoter. It contains the binding site for CpxR-P (phosphorylated CpxR). CpxR is one member of the two-component Cpx response to envelope stress. Briefly, envelope stress causes CpxA, an inner-membrane histidine Kinase to Phosphorylate CpxR, allowing it to bind to CpxR-P responsive regions and regulate downstream target genes. See 'Surface sensing and adhesion of Escherichia coli controlled by the Cpx-signaling pathway', Karen Otto and Thomas J. Silhavy, PNAS, Vol. 99., No, 4., 2287-2292. 2002.
discontinued: false
dominant: false
experience: experience/Works
group u list: _183_
m user id: 0
owner id: 2796
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: None
star: false
creator: Oliver Purcell
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: BBa_K135000
sequence annotation: CpxR-P Binding site
sequence: https://synbiohub.org/public/igem/BBa_K135000_sequence/1

BBa_K135000
type: SequenceAnnotation
persistent identity: annotation1982752
display id: annotation1982752
version: 1
top level: BBa_K135000
location: https://synbiohub.org/public/igem/BBa_K135000/annotation1982752/range1982752/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_K135000/annotation1982752/range1982752/1
type: Range
persistent identity: range1982752
display id: range1982752
version: 1
top level: BBa_K135000
direction: direction/forward
start: 1
end: 55
orientation: inline

CpxR-P Binding site
type: SequenceAnnotation
persistent identity: annotation1982751
display id: annotation1982751
version: 1
top level: BBa_K135000
location: https://synbiohub.org/public/igem/BBa_K135000/annotation1982751/range1982751/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_K135000/annotation1982751/range1982751/1
type: Range
persistent identity: range1982751
display id: range1982751
version: 1
top level: BBa_K135000
direction: direction/forward
start: 1
end: 15
orientation: inline

Si4
type: ComponentDefinition
persistent identity: BBa K1028000
display id: BBa_K1028000
version: 1
was generated by: iGEM to SBOL conversion
description: Silica binding peptide
created: 2013-08-29T11:00:00Z
modified: 2015-05-08T01:08:46Z
mutable provenance: This sequence is taken from the "Site-Speciﬁc Labeling of Surface Proteins on Living Cells Using Genetically Encoded Peptides that Bind Fluorescent Nanoparticle Probes" paper published by Rocco et al.
owned by: james
owned by: myers
mutable description: This is a short peptide of 15 amino acids designed to bind with high affinity to silica and silica-like particles, designed as a tag to be attached to the C-terminus of polypeptides. This part was originally designed to be conjugated to the C-terminal of Ice Nucleation Protein allowing extracellular expression of the binding region.
discontinued: false
dominant: false
experience: experience/None
group u list: _1335_
m user id: 0
owner id: 15658
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: The sequence was codon optomised for E.Coli expression.
star: false
creator: Oleg Karpov, Joseph Beton
type: DnaRegion
role: partType/Tag
role: SO:0000324
sequence annotation: Coding region
sequence annotation: stop codon
sequence: https://synbiohub.org/public/igem/BBa_K1028000_sequence/1

Coding region
type: SequenceAnnotation
persistent identity: annotation2333674
display id: annotation2333674
version: 1
top level: Si4
location: https://synbiohub.org/public/igem/BBa_K1028000/annotation2333674/range2333674/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_K1028000/annotation2333674/range2333674/1
type: Range
persistent identity: range2333674
display id: range2333674
version: 1
top level: Si4
direction: direction/forward
start: 1
end: 45
orientation: inline

stop codon
type: SequenceAnnotation
persistent identity: annotation2333672
display id: annotation2333672
version: 1
top level: Si4
location: https://synbiohub.org/public/igem/BBa_K1028000/annotation2333672/range2333672/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_K1028000/annotation2333672/range2333672/1
type: Range
persistent identity: range2333672
display id: range2333672
version: 1
top level: Si4
direction: direction/forward
start: 46
end: 46
orientation: inline

stop codon
type: SequenceAnnotation
persistent identity: annotation2333673
display id: annotation2333673
version: 1
top level: Si4
location: https://synbiohub.org/public/igem/BBa_K1028000/annotation2333673/range2333673/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_K1028000/annotation2333673/range2333673/1
type: Range
persistent identity: range2333673
display id: range2333673
version: 1
top level: Si4
direction: direction/forward
start: 49
end: 49
orientation: inline

BBa_K523008
type: ComponentDefinition
persistent identity: BBa K523008
display id: BBa_K523008
version: 1
was generated by: iGEM to SBOL conversion
description: INP for BioSandwich
created: 2011-08-03T11:00:00Z
modified: 2015-05-08T01:12:33Z
mutable provenance: From <partinfo>BBa_K265008</partinfo>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: This is a modified version of <partinfo>BBa_K265008</partinfo> for use with the BioSandwich protocol of Edinburgh 2011.
discontinued: false
dominant: false
experience: experience/None
group u list: _688_
m user id: 0
owner id: 8799
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: N/A.
star: false
creator: Mun Ching Lee, Sylvia Ispasanie
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: BglII
sequence annotation: ATG
sequence annotation: INPNC
sequence annotation: GG
sequence: https://synbiohub.org/public/igem/BBa_K523008_sequence/1

BglII
type: SequenceAnnotation
persistent identity: annotation2124325
display id: annotation2124325
version: 1
top level: BBa_K523008
location: https://synbiohub.org/public/igem/BBa_K523008/annotation2124325/range2124325/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_K523008/annotation2124325/range2124325/1
type: Range
persistent identity: range2124325
display id: range2124325
version: 1
top level: BBa_K523008
direction: direction/forward
start: 1
end: 4
orientation: inline

ATG
type: SequenceAnnotation
persistent identity: annotation2124326
display id: annotation2124326
version: 1
top level: BBa_K523008
location: https://synbiohub.org/public/igem/BBa_K523008/annotation2124326/range2124326/1
role: SO:0000318
role: feature/start

https://synbiohub.org/public/igem/BBa_K523008/annotation2124326/range2124326/1
type: Range
persistent identity: range2124326
display id: range2124326
version: 1
top level: BBa_K523008
direction: direction/forward
start: 5
end: 5
orientation: inline

INPNC
type: SequenceAnnotation
persistent identity: annotation2124328
display id: annotation2124328
version: 1
top level: BBa_K523008
location: https://synbiohub.org/public/igem/BBa_K523008/annotation2124328/range2124328/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_K523008/annotation2124328/range2124328/1
type: Range
persistent identity: range2124328
display id: range2124328
version: 1
top level: BBa_K523008
direction: direction/forward
start: 5
end: 928
orientation: inline

GG
type: SequenceAnnotation
persistent identity: annotation2124327
display id: annotation2124327
version: 1
top level: BBa_K523008
location: https://synbiohub.org/public/igem/BBa_K523008/annotation2124327/range2124327/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_K523008/annotation2124327/range2124327/1
type: Range
persistent identity: range2124327
display id: range2124327
version: 1
top level: BBa_K523008
direction: direction/forward
start: 929
end: 930
orientation: inline

BBa_B0030
type: ComponentDefinition
persistent identity: BBa B0030
display id: BBa_B0030
version: 1
was generated by: iGEM to SBOL conversion
description: RBS.1 (strong) -- modified from R. Weiss
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Strong RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>.
discontinued: false
dominant: true
experience: experience/Works
group u list: _44_46_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("orig" in figure 4-14 of Ron Weiss thesis). No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: partType/RBS
role: SO:0000139
sequence annotation: BBa_B0030
sequence annotation: RBS-1\Strong
sequence annotation: RBS
sequence: https://synbiohub.org/public/igem/BBa_B0030_sequence/1

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation7025
display id: annotation7025
version: 1
top level: BBa_B0030
location: https://synbiohub.org/public/igem/BBa_B0030/annotation7025/range7025/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0030/annotation7025/range7025/1
type: Range
persistent identity: range7025
display id: range7025
version: 1
top level: BBa_B0030
direction: direction/forward
start: 1
end: 15
orientation: inline

RBS-1\Strong
type: SequenceAnnotation
persistent identity: annotation1701
display id: annotation1701
version: 1
top level: BBa_B0030
location: https://synbiohub.org/public/igem/BBa_B0030/annotation1701/range1701/1
role: SO:0000139
role: feature/rbs

https://synbiohub.org/public/igem/BBa_B0030/annotation1701/range1701/1
type: Range
persistent identity: range1701
display id: range1701
version: 1
top level: BBa_B0030
direction: direction/forward
start: 1
end: 15
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation1702
display id: annotation1702
version: 1
top level: BBa_B0030
location: https://synbiohub.org/public/igem/BBa_B0030/annotation1702/range1702/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_B0030/annotation1702/range1702/1
type: Range
persistent identity: range1702
display id: range1702
version: 1
top level: BBa_B0030
direction: direction/forward
start: 8
end: 12
orientation: inline

GFP
type: ComponentDefinition
persistent identity: BBa E0040
display id: BBa_E0040
version: 1
was generated by: iGEM to SBOL conversion
description: green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (SwissProt: P42212
created: 2004-09-29T11:00:00Z
modified: 2016-01-26T02:09:38Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: GFP (mut3b) [note that this part does not have a barcode]
discontinued: false
dominant: true
experience: experience/Works
group u list: _11_1_
m user id: 4206
owner id: 61
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: jcbraff
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: GFP protein
sequence: https://synbiohub.org/public/igem/BBa_E0040_sequence/1

GFP protein
type: SequenceAnnotation
persistent identity: annotation1934520
display id: annotation1934520
version: 1
top level: GFP
location: https://synbiohub.org/public/igem/BBa_E0040/annotation1934520/range1934520/1
role: feature/protein
role: SO:0000316

https://synbiohub.org/public/igem/BBa_E0040/annotation1934520/range1934520/1
type: Range
persistent identity: range1934520
display id: range1934520
version: 1
top level: GFP
direction: direction/forward
start: 1
end: 720
orientation: inline

BBa_B1006
type: ComponentDefinition
persistent identity: BBa B1006
display id: BBa_B1006
version: 1
was generated by: iGEM to SBOL conversion
description: Terminator (artificial, large, %T~>90)
created: 2006-08-30T11:00:00Z
modified: 2015-08-31T04:07:21Z
mutable provenance: modified E. coli thr terminator, replaced all A-T pairs in stem with C-G pairs
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Artificial terminator, estimated %T~>90% *8bp stem, 6nt loop *Bidirectional, estimated reverse %T~>90%
discontinued: false
dominant: true
experience: experience/Works
group u list: _41_
m user id: 0
owner id: 745
owning group id: 41
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Bidirectional, with the reverse estimated to be less effective than the forward. Has a polyA tail of 9 residues.
star: true
creator: Haiyao Huang
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: B1006
sequence annotation: PolyA
sequence annotation: modified thr terminator
sequence: https://synbiohub.org/public/igem/BBa_B1006_sequence/1

B1006
type: SequenceAnnotation
persistent identity: annotation1898428
display id: annotation1898428
version: 1
top level: BBa_B1006
location: https://synbiohub.org/public/igem/BBa_B1006/annotation1898428/range1898428/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B1006/annotation1898428/range1898428/1
type: Range
persistent identity: range1898428
display id: range1898428
version: 1
top level: BBa_B1006
direction: direction/forward
start: 1
end: 39
orientation: inline

PolyA
type: SequenceAnnotation
persistent identity: annotation1898431
display id: annotation1898431
version: 1
top level: BBa_B1006
location: https://synbiohub.org/public/igem/BBa_B1006/annotation1898431/range1898431/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B1006/annotation1898431/range1898431/1
type: Range
persistent identity: range1898431
display id: range1898431
version: 1
top level: BBa_B1006
direction: direction/reverse
start: 1
end: 9
orientation: inline

modified thr terminator
type: SequenceAnnotation
persistent identity: annotation1898429
display id: annotation1898429
version: 1
top level: BBa_B1006
location: https://synbiohub.org/public/igem/BBa_B1006/annotation1898429/range1898429/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B1006/annotation1898429/range1898429/1
type: Range
persistent identity: range1898429
display id: range1898429
version: 1
top level: BBa_B1006
direction: direction/forward
start: 10
end: 31
orientation: inline

PolyA
type: SequenceAnnotation
persistent identity: annotation1898430
display id: annotation1898430
version: 1
top level: BBa_B1006
location: https://synbiohub.org/public/igem/BBa_B1006/annotation1898430/range1898430/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B1006/annotation1898430/range1898430/1
type: Range
persistent identity: range1898430
display id: range1898430
version: 1
top level: BBa_B1006
direction: direction/forward
start: 32
end: 39
orientation: inline

https://synbiohub.org/public/igem/BBa_K1028003_sequence/1
type: Sequence
persistent identity: BBa K1028003 sequence
display id: BBa_K1028003_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttgacagctagctcagtcctaggtataatgctagctactagagaaagaggagaaatactagagatctatgaccctggataaagcgctggtgctgcgcacttgtgccaataatatggccgaccattgtggcctgatctggcctgcttctggtacagttgaaagccgctattggcagtctacacgtcgtcacgaaaacgggctggttgggctgctgtggggtgccggaacatccgcctttctgagcgttcatgccgatgctcgttggattgtttgtgaggttgctgttgccgacatcattagcctggaagaaccgggtatggtgaaatttcctcgtgctgaagtggtccatgttggtgatcgtattagcgcctcccatttcatttcagctcgtcaggctgatccagcaagcacatcaacaagcacctctaccagtacactgacaccaatgccaaccgctatccctactcctatgccggctgtagcctctgttactctgcctgtggccgaacaagctcgccacgaagtctttgacgttgcctctgtgtctgctgctgctgctcctgttaacactctgcctgttaccacgcctcaaaatctgcaaacggcgacctatggctcaacactgagtggtgataaccattctcgtctgattgccgggtatggctctaacgaaaccgccggaaaccattctgatctgatcggtggtcacgattgtacgctgatggcaggtgatcaatctcgtctgaccgctggcaaaaattccgttctgaccgctggggcacgttctaaactgatcgggagcgaaggctctactctgtccgccggagaagattctaccctgatctttcgtctgtgggatggtaaacgttatcgtcagctggtcgctcgtacaggtgaaaatggtgtggaagccgacattccgtattatgtgaatgaggacgatgatattgtggacaaaccggacgaagatgatgattggatcgaagtgaaaggggcggtgggactcatcaccatcgccctcatccacatccgtcgatgtaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagaggtaaaacaacgtaaagtcatggattagcgacgtctgatgacgtaatttctgcctctactagagattaaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactaga…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0015_sequence/1
type: Sequence
persistent identity: BBa B0015 sequence
display id: BBa_B0015_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J23119_sequence/1
type: Sequence
persistent identity: BBa J23119 sequence
display id: BBa_J23119_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttgacagctagctcagtcctaggtataatgctagc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1028001_sequence/1
type: Sequence
persistent identity: BBa K1028001 sequence
display id: BBa_K1028001_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atctatgaccctggataaagcgctggtgctgcgcacttgtgccaataatatggccgaccattgtggcctgatctggcctgcttctggtacagttgaaagccgctattggcagtctacacgtcgtcacgaaaacgggctggttgggctgctgtggggtgccggaacatccgcctttctgagcgttcatgccgatgctcgttggattgtttgtgaggttgctgttgccgacatcattagcctggaagaaccgggtatggtgaaatttcctcgtgctgaagtggtccatgttggtgatcgtattagcgcctcccatttcatttcagctcgtcaggctgatccagcaagcacatcaacaagcacctctaccagtacactgacaccaatgccaaccgctatccctactcctatgccggctgtagcctctgttactctgcctgtggccgaacaagctcgccacgaagtctttgacgttgcctctgtgtctgctgctgctgctcctgttaacactctgcctgttaccacgcctcaaaatctgcaaacggcgacctatggctcaacactgagtggtgataaccattctcgtctgattgccgggtatggctctaacgaaaccgccggaaaccattctgatctgatcggtggtcacgattgtacgctgatggcaggtgatcaatctcgtctgaccgctggcaaaaattccgttctgaccgctggggcacgttctaaactgatcgggagcgaaggctctactctgtccgccggagaagattctaccctgatctttcgtctgtgggatggtaaacgttatcgtcagctggtcgctcgtacaggtgaaaatggtgtggaagccgacattccgtattatgtgaatgaggacgatgatattgtggacaaaccggacgaagatgatgattggatcgaagtgaaaggggcggtgggactcatcaccatcgccctcatccacatccgtcgatgtaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1028002_sequence/1
type: Sequence
persistent identity: BBa K1028002 sequence
display id: BBa_K1028002_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gtaaaacaacgtaaagtcatggattagcgacgtctgatgacgtaatttctgcctctactagagattaaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagaaaaaaaaaccccgcccctgacagggcggggtttttttt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K081012_sequence/1
type: Sequence
persistent identity: BBa K081012 sequence
display id: BBa_K081012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: attaaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagaaaaaaaaaccccgcccctgacagggcggggtttttttt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K135000_sequence/1
type: Sequence
persistent identity: BBa K135000 sequence
display id: BBa_K135000_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gtaaaacaacgtaaagtcatggattagcgacgtctgatgacgtaatttctgcctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1028000_sequence/1
type: Sequence
persistent identity: BBa K1028000 sequence
display id: BBa_K1028000_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ggcggtgggactcatcaccatcgccctcatccacatccgtcgatgtaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K523008_sequence/1
type: Sequence
persistent identity: BBa K523008 sequence
display id: BBa_K523008_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atctatgaccctggataaagcgctggtgctgcgcacttgtgccaataatatggccgaccattgtggcctgatctggcctgcttctggtacagttgaaagccgctattggcagtctacacgtcgtcacgaaaacgggctggttgggctgctgtggggtgccggaacatccgcctttctgagcgttcatgccgatgctcgttggattgtttgtgaggttgctgttgccgacatcattagcctggaagaaccgggtatggtgaaatttcctcgtgctgaagtggtccatgttggtgatcgtattagcgcctcccatttcatttcagctcgtcaggctgatccagcaagcacatcaacaagcacctctaccagtacactgacaccaatgccaaccgctatccctactcctatgccggctgtagcctctgttactctgcctgtggccgaacaagctcgccacgaagtctttgacgttgcctctgtgtctgctgctgctgctcctgttaacactctgcctgttaccacgcctcaaaatctgcaaacggcgacctatggctcaacactgagtggtgataaccattctcgtctgattgccgggtatggctctaacgaaaccgccggaaaccattctgatctgatcggtggtcacgattgtacgctgatggcaggtgatcaatctcgtctgaccgctggcaaaaattccgttctgaccgctggggcacgttctaaactgatcgggagcgaaggctctactctgtccgccggagaagattctaccctgatctttcgtctgtgggatggtaaacgttatcgtcagctggtcgctcgtacaggtgaaaatggtgtggaagccgacattccgtattatgtgaatgaggacgatgatattgtggacaaaccggacgaagatgatgattggatcgaagtgaaagg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0030_sequence/1
type: Sequence
persistent identity: BBa B0030 sequence
display id: BBa_B0030_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: attaaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E0040_sequence/1
type: Sequence
persistent identity: BBa E0040 sequence
display id: BBa_E0040_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B1006_sequence/1
type: Sequence
persistent identity: BBa B1006 sequence
display id: BBa_B1006_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaaaaaaaaccccgcccctgacagggcggggtttttttt
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z