LOCUS       BBa_K103017             1328 bp    DNA     linear       22-Nov-2024
DEFINITION  OmpA_linker_alpha_linker under Plac
ACCESSION   BBa_K103017
VERSION     BBa_K103017.1  
FEATURES             Location/Qualifiers
     misc_feature    52..77
                     /label=CAP binding site
     misc_feature    117..151
                     /label=LacI binding site
     promoter        112..117
                     /label=-10
     promoter        94..100
                     /label=-35
     RBS             271..274
                     /label=RBS
     promoter        1..279
                     /label=BBa_K103022
     misc_feature    280..286
                     /label=NdeI
     CDS             283..717
                     /label=OmpA
     CDS             718..743
                     /label=linker
     misc_feature    738..743
                     /label=SacI
     misc_feature    283..285
                     /label=ATG
     misc_feature    280..743
                     /label=BBa_K103006
     CDS             744..1260
                     /label=alpha
     CDS             1261..1328
                     /label=linker
     misc_feature    1321..1326
                     /label=BamHI
     CDS             744..1328
                     /label=BBa_K103008
ORIGIN
        1 ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag
       61 ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg
      121 tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg attacgaatt
      181 aattctcact cattaggcac cccaggcttt acactttatg cttccggctc gtataatgtg
      241 tggaattgtg agcggataac aatttcacac aggaaacagc atatgaaagc tactaaactg
      301 gtactgggcg cggtaatcct gggttctact ctgctggcag gttgctccag caacgctaaa
      361 atcgatcagg gaattaaccc gtatgttggc tttgaaatgg gttacgactg gttaggtcgt
      421 atgccgtaca aaggcagcgt tgaaaacggt gcatacaaag ctcagggcgt tcaactgacc
      481 gctaaactgg gttacccaat cactgacgac ctggacatct acactcgtct gggtggcatg
      541 gtatggcgtg cagacactaa atccaacgtt tatggtaaaa accacgacac cggcgtttct
      601 ccggtcttcg ctggcggtgt tgagtacgcg atcactcctg aaatcgctac ccgtctggaa
      661 taccagtgga ccaacaacat cggtgacgca cacaccatcg gcactcgtcc ggacaacggc
      721 ggaggttctg gaggagggag ctctcaccca gaaacgctgg tgaaagtaaa agatgctgaa
      781 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt
      841 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt
      901 ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat
      961 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg
     1021 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta
     1081 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat
     1141 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag
     1201 cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa
     1261 ggtggcggag gaagcggagg aggtggttct ggaggtggag gtagcggtgg aggaggttct
     1321 ggatccgg
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