LOCUS       BBa_K103019              744 bp    DNA     linear       23-Dec-2024
DEFINITION  alpha_linker under PT7
ACCESSION   BBa_K103019
VERSION     BBa_K103019.1  
FEATURES             Location/Qualifiers
     promoter        12..28
                     /label=T7
     misc_feature    32..57
                     /label=lacO
     RBS             92..96
                     /label=rbs
     misc_feature    116..130
                     /label=HisTag
     misc_feature    143..160
                     /label=thrombin
     CDS             104..166
                     /label=HisTagged ORF
     misc_feature    161..166
                     /label=NdeI
     misc_feature    102..107
                     /label=NcoI
     misc_feature    104..106
                     /label=ATG
     misc_feature    164..166
                     /label=ATG
     promoter        1..166
                     /label=BBa_K103021
     CDS             167..225
                     /label=linker
     misc_feature    168..173
                     /label=SacI
     CDS             226..744
                     /label=alpha
     misc_feature    742..744
                     /label=stop
     misc_feature    167..744
                     /label=BBa_K103009
ORIGIN
        1 atcccgcgaa attaatacga ctcactatag gggaattgtg agcggataac aattcccctc
       61 tagctagaaa taattttgtt taactttaag aaggagatat accatgggca gcagccatca
      121 tcatcatcat cacagcagcg gcctggtgcc gcgcggcagc catatgggag ctctggtgga
      181 agcggtggcg gaggatctgg aggaggtggt tctggaggtg gaggtcaccc agaaacgctg
      241 gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat
      301 ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc
      361 acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa
      421 ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa
      481 aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt
      541 gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct
      601 tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat
      661 gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg
      721 cgcaaactat taactggcga ataa
//