##gff-version 3
##sequence-region BBa_K103019 1 744
BBa_K103019	.	promoter	1	166	.	+	0	ID=BBa_K103021;Name=BBa_K103021
BBa_K103019	.	operator	32	57	.	+	0	ID=annotation1979833;Name=lacO;Parent=BBa_K103021
BBa_K103019	.	sequence_feature	161	166	.	+	0	ID=annotation1980391;Name=NdeI;Parent=BBa_K103021
BBa_K103019	.	CDS	104	166	.	+	0	ID=annotation1979837;Name=HisTagged ORF;Parent=BBa_K103021
BBa_K103019	.	sequence_feature	143	160	.	+	0	ID=annotation1979836;Name=thrombin;Parent=BBa_K103021
BBa_K103019	.	tag	116	130	.	+	0	ID=annotation1979835;Name=HisTag;Parent=BBa_K103021
BBa_K103019	.	sequence_feature	102	107	.	+	0	ID=annotation1993557;Name=NcoI;Parent=BBa_K103021
BBa_K103019	.	ribosome_entry_site	92	96	.	+	0	ID=annotation1979834;Name=rbs;Parent=BBa_K103021
BBa_K103019	.	start_codon	164	166	.	+	0	ID=annotation1993574;Name=ATG;Parent=BBa_K103021
BBa_K103019	.	start_codon	104	106	.	+	0	ID=annotation1993573;Name=ATG;Parent=BBa_K103021
BBa_K103019	.	promoter	12	28	.	+	0	ID=annotation1979832;Name=T7;Parent=BBa_K103021
BBa_K103019	.	polypeptide_domain	167	744	.	+	0	ID=BBa_K103009;Name=BBa_K103009
BBa_K103019	.	CDS	226	744	.	+	0	ID=annotation1986313;Name=alpha;Parent=BBa_K103009
BBa_K103019	.	CDS	167	225	.	+	0	ID=annotation1986311;Name=linker;Parent=BBa_K103009
BBa_K103019	.	sequence_feature	168	173	.	+	0	ID=annotation1986312;Name=SacI;Parent=BBa_K103009
BBa_K103019	.	stop_codon	742	744	.	+	0	ID=annotation1986314;Name=stop;Parent=BBa_K103009
>BBa_K103019
atcccgcgaaattaatacgactcactataggggaattgtgagcggataacaattcccctctagctagaaataattttgt
ttaactttaagaaggagatataccatgggcagcagccatcatcatcatcatcacagcagcggcctggtgccgcgcggca
gccatatgggagctctggtggaagcggtggcggaggatctggaggaggtggttctggaggtggaggtcacccagaaacg
ctggtgaaagtaaaagatgctgaagatcagttgggtgcacgagtgggttacatcgaactggatctcaacagcggtaaga
tccttgagagttttcgccccgaagaacgttttccaatgatgagcacttttaaagttctgctatgtggcgcggtattatc
ccgtattgacgccgggcaagagcaactcggtcgccgcatacactattctcagaatgacttggttgagtactcaccagtc
acagaaaagcatcttacggatggcatgacagtaagagaattatgcagtgctgccataaccatgagtgataacactgcgg
ccaacttacttctgacaacgatcggaggaccgaaggagctaaccgcttttttgcacaacatgggggatcatgtaactcg
ccttgatcgttgggaaccggagctgaatgaagccataccaaacgacgagcgtgacaccacgatgcctgtagcaatggca
acaacgttgcgcaaactattaactggcgaataa