LOCUS       BBa_K103021              166 bp    DNA     linear       22-Nov-2024
DEFINITION  modified T7 promoter with His-Tag
ACCESSION   BBa_K103021
VERSION     BBa_K103021.1  
FEATURES             Location/Qualifiers
     promoter        12..28
                     /label=T7
     misc_feature    32..57
                     /label=lacO
     RBS             92..96
                     /label=rbs
     misc_feature    116..130
                     /label=HisTag
     misc_feature    143..160
                     /label=thrombin
     CDS             104..166
                     /label=HisTagged ORF
     misc_feature    161..166
                     /label=NdeI
     misc_feature    102..107
                     /label=NcoI
     misc_feature    104..106
                     /label=ATG
     misc_feature    164..166
                     /label=ATG
ORIGIN
        1 atcccgcgaa attaatacga ctcactatag gggaattgtg agcggataac aattcccctc
       61 tagctagaaa taattttgtt taactttaag aaggagatat accatgggca gcagccatca
      121 tcatcatcat cacagcagcg gcctggtgcc gcgcggcagc catatg
//