BBa_K1033003 1 BBa_K1033003 B0034-4Cl-B0034-STS 2013-09-13T11:00:00Z 2015-05-08T01:08:48Z Comes from vitis vinifiera and arabidopsis thaliana A assembly of our two biobricks, 4Cl and STS. false false _1340_ 0 13994 9 It's complicated false We coupled them together with the same rbs for simultaneous expression. false Hampus Elofsson component2341490 1 BBa_K1033001 component2341491 1 BBa_K1033002 annotation2341491 1 BBa_K1033002 range2341491 1 1717 2916 annotation2341490 1 BBa_K1033001 range2341490 1 1 1708 BBa_K1033002 1 BBa_K1033002 stilbene synthase (STS) with RBS 2013-08-20T11:00:00Z 2015-05-08T01:08:48Z Vitis vinifera This part codes for the enzyme stilbene synthase (STS). Stilbene synthase is the third and last enzyme in the stilbene synthesis pathway which derrives from the universal polyphenol biosynthetic pathway. It catalyzes the formation of resveratrol, using the substrates coumaryl-CoA and malonyl-CoA.[1] 1. Chin Giaw Lim, Zachary L. Fowler, Thomas Hueller, Steffen Schaffer, Mattheos A. G. Koffas (2011) High-Yield Resveratrol Production in Engineered Escherichia coli. Appl Environ Microbiol 77(10):3451 false false _1340_ 0 17930 9 It's complicated false We chose to work with an eukaryote STS from an organism which has previously been shown to function in Escherichia coli. false Lovisa Pettersson annotation2367782 1 B0034 range2367782 1 1 8 annotation2367772 1 Stilbene synthase range2367772 1 19 1200 BBa_K1033001 1 BBa_K1033001 4-coumarate ligase (4CL) with RBS 2013-08-22T11:00:00Z 2015-05-08T01:08:48Z Arabidopsis Thaliana 4-coumarate ligase (4CL) is an enzyme that catalyses the reaction from p-coumaric acid to 4-coumaroyl-coenzyme A (4-coumaryl-CoA). false false _1340_ 0 18317 9 It's complicated false Yes. false Karl Holdar annotation2368408 1 4-coumarate-ligase range2368408 1 23 1701 annotation2368409 1 B0034 range2368409 1 4 11 BBa_K1033002_sequence 1 aaagaggagaaaactggaatggcttcagtcgaggaatttagaaacgctcaacgtgccaagggtccggccaccatcctagccattggcacagctacccccgaccactgtgtctaccagtctgattatgctgattactatttcagggtcactaagagcgagcacatgatgactgagttgaagaagaagttcaatcgcatatgtgacaaatcaatgatcaagaagcgttacattcacttgaccgaagaaatgcttgaggagcacccaaacattggtgcttatatggctccatctcttaacatacgccaagagattattactgctgaggtacctagacttggtagggatgcagcattgaaggctcttaaagagtggggccaaccaaagtccaagatcacccatcttgtattttgtacaacctccggtgtagaaatgcccggtgcggattacaaacttgctaatctcttaggtcttgaaacatcggttagaagggtgatgttgtaccatcaagggtgctatgcaggtggaactgtccttcgaactgctaaggatcttgcagaaaataatgcaggagcacgagttcttgtggtgtgctctgagatcactgttgttacattccgtgggccttccgaagatgctttggactctttagttggccaagccctttttggtgatgggtcttcagctgtgattgttggatcagatccagatgtctcgattgaacgaccactcttccaacttgtttcagcagcccaaacatttattcctaattcagcaggagccattgccggaaacttacgtgaggtggggctcacctttcatttgtggcccaatgtgcctactttgatttctgagaacatagagaaatgcttgacccaggcttttgacccacttggtattagcgattggaactcattattttggattgctcacccaggtggccctgcaattctcgatgcagttgaagcaaaactcaatttagagaaaaagaaacttgaagcaacaaggcacgtgttaagtgagtacggtaacatgtcaagtgcatgtgtgttgtttattttggatgagatgagaaagaaatccctaaagggggaaaaagccaccactggtgaaggattggattggggagtactatttggttttgggccaggcttgaccatcgaaactgttgtgctacatagcattcctatggttacaaattaa BBa_K1033001_sequence 1 gacaaaagaggagaaatctcctatggcgccacaagaacaagcagtttctcaggtgatggagaaacagagcaacaacaacaacagtgacgtcattttccgatcaaagttaccggatatttacatcccgaaccacctatctctccacgactacatcttccaaaacatctccgaatttgccactaagccttgcctaatcaacggaccaaccggccacgtgtacacttactccgacgtccacgtcatctcccgccaaatcgccgccaattttcacaaactcggcgttaaccaaaacgacgtcgtcatgctcctcctcccaaactgtcccgaatttgtcctctctttcctcgccgcctccttccgcggcgcaaccgccaccgccgcaaaccctttcttcactccggcggagatagctaaacaagccaaagcctccaacaccaaactcataatcaccgaagctcgttacgtcgacaaaatcaaaccacttcaaaacgacgacggagtagtcatcgtctgcatcgacgacaacgaatccgtgccaatccctgaaggctgcctccgcttcaccgagttgactcagtcgacaaccgaggcatcagaagtcatcgactcggtggagatttcaccggacgacgtggtggcactaccttactcctctggcacgacgggattaccaaaaggagtgatgctgactcacaagggtctagtcacgagcgttgctcagcaagtcgacggcgagaacccgaatctttatttccacagcgatgacgtcatactctgtgttttgcccatgtttcatatctacgctttgaactcgatcatgttgtgtggtcttagagttggtgcggcgattctgataatgccgaagtttgagatcaatctgctattggagctgatccagaggtgtaaagtgacggtggctccgatggttccgccgattgtgttggccattgcgaagtcttcggagacggagaagtatgatttgagctcgataagagtggtgaaatctggtgctgctcctcttggtaaagaacttgaagatgccgttaatgccaagtttcctaatgccaaactcggtcagggatacggaatgacggaagcaggtccagtgctagcaatgtcgttaggttttgcaaaggaaccttttccggttaagtcaggagcttgtggtactgttgtaagaaatgctgagatgaaaatagttgatccagacaccggagattctctttcgaggaatcaacccggtgagatttgtattcgtggtcaccagatcatgaaaggttacctcaacaatccggcagctacagcagagaccattgataaagacggttggcttcatactggagatattggattgatcgatgacgatgacgagcttttcatcgttgatcgattgaaagaacttatcaagtataaaggttttcaggtagctccggctgagctagaggctttgctcatcggtcatcctgacattactgatgttgctgttgtcgcaatgaaagaagaagcagctggtgaagttcctgttgcatttgtggtgaaatcgaaggattcggagttatcagaagatgatgtgaagcaattcgtgtcgaaacaggttgtgttttacaagagaatcaacaaagtgttcttcactgaatccattcctaaagctccatcagggaagatattgaggaaagatctgagggcaaaactagcaaatggattgtaa BBa_K1033003_sequence 1 gacaaaagaggagaaatctcctatggcgccacaagaacaagcagtttctcaggtgatggagaaacagagcaacaacaacaacagtgacgtcattttccgatcaaagttaccggatatttacatcccgaaccacctatctctccacgactacatcttccaaaacatctccgaatttgccactaagccttgcctaatcaacggaccaaccggccacgtgtacacttactccgacgtccacgtcatctcccgccaaatcgccgccaattttcacaaactcggcgttaaccaaaacgacgtcgtcatgctcctcctcccaaactgtcccgaatttgtcctctctttcctcgccgcctccttccgcggcgcaaccgccaccgccgcaaaccctttcttcactccggcggagatagctaaacaagccaaagcctccaacaccaaactcataatcaccgaagctcgttacgtcgacaaaatcaaaccacttcaaaacgacgacggagtagtcatcgtctgcatcgacgacaacgaatccgtgccaatccctgaaggctgcctccgcttcaccgagttgactcagtcgacaaccgaggcatcagaagtcatcgactcggtggagatttcaccggacgacgtggtggcactaccttactcctctggcacgacgggattaccaaaaggagtgatgctgactcacaagggtctagtcacgagcgttgctcagcaagtcgacggcgagaacccgaatctttatttccacagcgatgacgtcatactctgtgttttgcccatgtttcatatctacgctttgaactcgatcatgttgtgtggtcttagagttggtgcggcgattctgataatgccgaagtttgagatcaatctgctattggagctgatccagaggtgtaaagtgacggtggctccgatggttccgccgattgtgttggccattgcgaagtcttcggagacggagaagtatgatttgagctcgataagagtggtgaaatctggtgctgctcctcttggtaaagaacttgaagatgccgttaatgccaagtttcctaatgccaaactcggtcagggatacggaatgacggaagcaggtccagtgctagcaatgtcgttaggttttgcaaaggaaccttttccggttaagtcaggagcttgtggtactgttgtaagaaatgctgagatgaaaatagttgatccagacaccggagattctctttcgaggaatcaacccggtgagatttgtattcgtggtcaccagatcatgaaaggttacctcaacaatccggcagctacagcagagaccattgataaagacggttggcttcatactggagatattggattgatcgatgacgatgacgagcttttcatcgttgatcgattgaaagaacttatcaagtataaaggttttcaggtagctccggctgagctagaggctttgctcatcggtcatcctgacattactgatgttgctgttgtcgcaatgaaagaagaagcagctggtgaagttcctgttgcatttgtggtgaaatcgaaggattcggagttatcagaagatgatgtgaagcaattcgtgtcgaaacaggttgtgttttacaagagaatcaacaaagtgttcttcactgaatccattcctaaagctccatcagggaagatattgaggaaagatctgagggcaaaactagcaaatggattgtaatactagagaaagaggagaaaactggaatggcttcagtcgaggaatttagaaacgctcaacgtgccaagggtccggccaccatcctagccattggcacagctacccccgaccactgtgtctaccagtctgattatgctgattactatttcagggtcactaagagcgagcacatgatgactgagttgaagaagaagttcaatcgcatatgtgacaaatcaatgatcaagaagcgttacattcacttgaccgaagaaatgcttgaggagcacccaaacattggtgcttatatggctccatctcttaacatacgccaagagattattactgctgaggtacctagacttggtagggatgcagcattgaaggctcttaaagagtggggccaaccaaagtccaagatcacccatcttgtattttgtacaacctccggtgtagaaatgcccggtgcggattacaaacttgctaatctcttaggtcttgaaacatcggttagaagggtgatgttgtaccatcaagggtgctatgcaggtggaactgtccttcgaactgctaaggatcttgcagaaaataatgcaggagcacgagttcttgtggtgtgctctgagatcactgttgttacattccgtgggccttccgaagatgctttggactctttagttggccaagccctttttggtgatgggtcttcagctgtgattgttggatcagatccagatgtctcgattgaacgaccactcttccaacttgtttcagcagcccaaacatttattcctaattcagcaggagccattgccggaaacttacgtgaggtggggctcacctttcatttgtggcccaatgtgcctactttgatttctgagaacatagagaaatgcttgacccaggcttttgacccacttggtattagcgattggaactcattattttggattgctcacccaggtggccctgcaattctcgatgcagttgaagcaaaactcaatttagagaaaaagaaacttgaagcaacaaggcacgtgttaagtgagtacggtaacatgtcaagtgcatgtgtgttgtttattttggatgagatgagaaagaaatccctaaagggggaaaaagccaccactggtgaaggattggattggggagtactatttggttttgggccaggcttgaccatcgaaactgttgtgctacatagcattcctatggttacaaattaa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z