BBa_K1033120 1 BBa_K1033120 B0034-ExpTag-Linker-Miraculin-Linker-6xHis 2013-09-01T11:00:00Z 2015-06-11T08:28:53Z Richadella Dulcifica, but codon optimized for E.coli only the miraculin is codon optimized. The codon usage for the export tag USP45's is best suited for Lactobacillus strains Construct needed to produce the protein miraculin, but fitted with a, export tag for transportation out of the cell, and a histidine tag (6x His) for purification purposes. false false _1340_ 4206 18316 9 In stock false The miraculin sequence is codon optimized for E.coli. The codon usage for the export tag USP45's is best suited for Lactobacillus strains false Magnus Bergman, Pontus Danielsson, Alexander Wirtanen, Nils Anlind, Christoffer Frisk, Thorsteinn Olaufsson, Malin Borg, Niclas Sandberg annotation2340531 1 Linker range2340531 1 100 108 annotation2334586 1 USP45 range2334586 1 18 99 annotation2340532 1 His-tag range2340532 1 691 714 annotation2340530 1 Linker range2340530 1 682 690 annotation2334587 1 B0034 range2334587 1 1 12 annotation2340533 1 Miraculin range2340533 1 109 681 BBa_K1033120_sequence 1 tataaagaggagaaatatacgatgaaaaaaaagattatctcagctattttaatgtctacagtgatactttctgctgctgcaccgttgtcaggtgtttacgctagtggaagtgattcagcccctaacccggtgttggatattgatggagaaaaattacgcacgggaactaactattacattgtaccagtattgcgcgatcatggtggcggacttacagtgagtgcgactaccccaaatggcacgtttgtatgtccaccgcgcgttgtgcaaacacgtaaagaagttgatcacgatcgcccactggctttctttccggaaaaccctaaggaagatgtagttcgtgtgagtactgatttaaacattaacttcagtgcttttatgccatgccgctggacaagttcaacggtttggcgtttggataagtacgatgaaagtactggtcaatactttgtgaccattggtggcgtaaaaggcaatcctggaccagaaactattagctcttggttcaagattgaagaattttgtggaagcggtttttataagttagttttctgccctaccgtgtgtggatcttgcaaagtaaagtgtggtgatgttggcatttacattgatcagaaaggtcgtcgccgtctggctttatcagataagccgtttgcctttgaatttaataagactgtttacttctcaggttctcatcaccatcatcaccattaataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z