LOCUS       BBa_K1033206            3057 bp    DNA     linear       22-Dec-2024
DEFINITION  Lactobacillus shuttle vector pSBLbC
ACCESSION   BBa_K1033206
VERSION     BBa_K1033206.1  
FEATURES             Location/Qualifiers
     misc_feature    1..21
                     /label=BioBrick suffix
     misc_feature    35..46
                     /label=Translational stop sequence (BBa B0042)
     stem_loop       47..115
                     /label=tonB derived terminator (BBa B0054)
     misc_feature    189..1980
                     /label=pSH71 replicon
     CDS             1028..1726
                     /label=RepA
     stem_loop       2010..2055
                     /label=Lux terminator
     misc_feature    2062..2865
                     /label=Chloramphenicol resistance cassette
     CDS             2062..2724
                     /label=cmR
     promoter        2787..2845
                     /label=CP29 (BBa K1033222)
     stem_loop       2872..2912
                     /label=P22 late terminator
     stem_loop       2933..3010
                     /label=T3 derived terminator (BBa B0055)
     misc_feature    3011..3022
                     /label=Translational stop sequence (BBa B0042)
     misc_feature    3036..3057
                     /label=BioBrick prefix
ORIGIN
        1 tactagtagc ggccgctgca ggagtcacta agggttagtt agttagatta gcagaaagtc
       61 aaaagcctcc gaccggaggc ttttgactaa aacttccctt ggggttatca ttggggctca
      121 ctcaaaggcg gtaatcagat aaaaaaaatc cttagctttc gctaggatga tttctgctag
      181 agacgcgtca gaatgtccga gactaattca tggatcgatt ttttattaaa acgtctcaaa
      241 atcgtttctg agacgtttta gcgtttattt cgtttagtta tcggcataat cgttaaaaca
      301 ggcgttatcg tagcgtaaaa gcccttgagc gtagcgtggc tttgcagcga agatgttgtc
      361 tgttagatta tgaaagccga tgactgaatg aaataataag cgcagcgtcc ttctatttct
      421 gttggaggag gctcaaggga gtttgaggga atgaaattcc ctcatgggtt tgattttaaa
      481 aattgcttgc aattttgtcg agcggtagcg ctggaaaatt tttgaaaaaa atttggaatt
      541 tggaaaaaaa tggggggaaa ggaagcgaat tttgcttccg tactacgacc ccccattaag
      601 tgccgagtgc caatttttgt gccaaaaacg ctctatccca actggctcaa gggtttgagg
      661 ggtttttcaa tcgccaacga atcgccaacg ttttcgccaa cgttttttat aaatctatat
      721 ttaagtagct ttattgttgt ttttatgatt acaaagtgat acactaattt tataaaatta
      781 tttgattgga gttttttaaa tggtgatttc agaatcgaaa aaaagagtta tgatttctct
      841 gacaaaagag caagataaaa aattaacaga tatggcgaaa caaaaaggtt tttcaaaatc
      901 tgcggttgcg gcgttagcta tagaagaata tgcaagaaag gaatcagaac aaaaaaaata
      961 agcgaaagct cgcgttttta gaaggatacg agttttcgct acttgttttt gataaggtaa
     1021 ttatatcatg gctattaaaa atactaaagc tagaaatttt ggatttttat tatatcctga
     1081 ctcaattcct aatgattgga aagaaaaatt agagagtttg ggcgtatcta tggctgtcag
     1141 tcctttacac gatatggacg aaaaaaaaga taaagataca tggaatagta gtgatgttat
     1201 acgaaatgga aagcactata aaaaaccaca ctatcacgtt atatatattg cacgaaatcc
     1261 tgtaacaata gaaagcgtta ggaacaagat taagcgaaaa ttggggaata gttcagttgc
     1321 tcatgttgag atacttgatt atatcaaagg ttcatatgaa tatttgactc atgaatcaaa
     1381 ggacgctatt gctaagaata aacatatata cgacaaaaaa gatattttga acattaatga
     1441 ttttgatatt gaccgctata taacacttga tgaaagccaa aaaagagaat tgaagaattt
     1501 acttttagat atagtggatg actataattt ggtaaataca aaagatttaa tggcttttat
     1561 tcgccttagg ggagcggagt ttggaatttt aaatacgaat gatgtaaaag atattgtttc
     1621 aacaaactct agcgccttta gattatggtt tgagggcaat tatcagtgtg gatatagagc
     1681 aagttatgca aaggttcttg atgctgaaac gggggaaata aaatgacaaa caaagaaaaa
     1741 gagttatttg ctgaaaatga ggaattaaaa aaagaaatta aggacttaaa agagcgtatt
     1801 gaaagataca gagaaatgga agttgaatta agtacaacaa tagatttatt gagaggaggg
     1861 attattgaat aaataaaagc ccccctgacg aaagtcgaag ggggctttta ttttggtttg
     1921 atgttgcgat taatagcaat acgattgcaa taaacaaaag gatcccctta gaagcaaact
     1981 gctagcgcca cacaaccaca ccacaccaca aataataaaa aagccggatt aataatctgg
     2041 ctttttatat tctctgtcga cttattacgc cccgccctgc cactcatcac agtactgttg
     2101 taattcatta agcatgcggc cgacatggaa gccatcacaa acggcatgat gaacctggat
     2161 cgccagcggc atcagcacct tgtcgccttg cgtataatat ttgcccatcg tgaaaacggg
     2221 ggcgaagaag ttgtccatat tggccacgtt taaatcaaaa ctggtgaaac tcacccaggg
     2281 attggctgag acaaaaaaca tattctcaat aaacccttta gggaaatagg ccaggttttc
     2341 accgtaacac gccacatctt gcgaatatat gtggagaaac tgccggaaat cgtcgtggta
     2401 ttcactccag agggacgaaa acgtttcagt ttgctcatgg aaaacggtgt aacaagggtg
     2461 aacactatcc cagatcacca gctcaccgtc tttcatggcc atacgaaact ccgggtgagc
     2521 gttcatcagg cgggcaagaa tgtgaataaa ggccggataa aacttgtgct tatttttctt
     2581 tacggtcttt aaaaaggccg taatatccag ctgaacggtc tggttatagg tacattgagc
     2641 aactgactga aatgcctcaa aatgttcttt acgatgccat tgggatatat caacggtggt
     2701 atatcccgtg atttttttct ccattttagc ttccttagct cctgaaaatc tcgataactc
     2761 aaaaaatacg cccggtagtg atcttaaaca gtactcagtt attataccac gtcccccctg
     2821 agatgtcaag aataacttac cgatgctctt acgtgcccga tcaacgagct caaataaagc
     2881 cctgagttta accgctcggg gctttttgcg tttgccacct gacgtctaag aaaaggaata
     2941 ttcagcaatt tgcccgtgcc gaagaaaggc ccacccgtga aggtgagcca gtgagttgat
     3001 tgctacgtaa ttagttagtt agcccttagt gactcgaatt cgcggccgct tctagag
//