LOCUS       BBa_K1033207            3307 bp    DNA     linear       20-Sep-2024
DEFINITION  Shuttle vector pSBLbE for E. coli and Lactobacillus
ACCESSION   BBa_K1033207
VERSION     BBa_K1033207.1  
FEATURES             Location/Qualifiers
     misc_feature    1..21
                     /label=BioBrick suffix
     misc_feature    35..46
                     /label=Translational stop sequence (BBa B0042)
     stem_loop       47..115
                     /label=tonB derived terminator (BBa B0054)
     misc_feature    183..1986
                     /label=pSH71 replicon
     CDS             1028..1726
                     /label=RepA
     stem_loop       2009..2055
                     /label=Lux terminator
     misc_feature    2056..3121
                     /label=Erythromycin resistance cassette
     CDS             2072..2806
                     /label=ermB
     RBS             2812..2817
                     /label=RBS
     CDS             2903..2986
                     /label=ermbI (regulatory protein)
     RBS             2994..2999
                     /label=RBS
     promoter        3057..3084
                     /label=Promoter
     stem_loop       3122..3162
                     /label=P22 late terminator
     stem_loop       3183..3260
                     /label=T3 terminator (BBa B0055)
     misc_feature    3261..3272
                     /label=Translational stop sequence (BBa B0042)
     misc_feature    3286..3307
                     /label=BioBrick prefix
ORIGIN
        1 tactagtagc ggccgctgca ggagtcacta agggttagtt agttagatta gcagaaagtc
       61 aaaagcctcc gaccggaggc ttttgactaa aacttccctt ggggttatca ttggggctca
      121 ctcaaaggcg gtaatcagat aaaaaaaatc cttagctttc gctaggatga tttctgctag
      181 agacgcgtca gaatgtccga gactaattca tggatcgatt ttttattaaa acgtctcaaa
      241 atcgtttctg agacgtttta gcgtttattt cgtttagtta tcggcataat cgttaaaaca
      301 ggcgttatcg tagcgtaaaa gcccttgagc gtagcgtggc tttgcagcga agatgttgtc
      361 tgttagatta tgaaagccga tgactgaatg aaataataag cgcagcgtcc ttctatttct
      421 gttggaggag gctcaaggga gtttgaggga atgaaattcc ctcatgggtt tgattttaaa
      481 aattgcttgc aattttgtcg agcggtagcg ctggaaaatt tttgaaaaaa atttggaatt
      541 tggaaaaaaa tggggggaaa ggaagcgaat tttgcttccg tactacgacc ccccattaag
      601 tgccgagtgc caatttttgt gccaaaaacg ctctatccca actggctcaa gggtttgagg
      661 ggtttttcaa tcgccaacga atcgccaacg ttttcgccaa cgttttttat aaatctatat
      721 ttaagtagct ttattgttgt ttttatgatt acaaagtgat acactaattt tataaaatta
      781 tttgattgga gttttttaaa tggtgatttc agaatcgaaa aaaagagtta tgatttctct
      841 gacaaaagag caagataaaa aattaacaga tatggcgaaa caaaaaggtt tttcaaaatc
      901 tgcggttgcg gcgttagcta tagaagaata tgcaagaaag gaatcagaac aaaaaaaata
      961 agcgaaagct cgcgttttta gaaggatacg agttttcgct acttgttttt gataaggtaa
     1021 ttatatcatg gctattaaaa atactaaagc tagaaatttt ggatttttat tatatcctga
     1081 ctcaattcct aatgattgga aagaaaaatt agagagtttg ggcgtatcta tggctgtcag
     1141 tcctttacac gatatggacg aaaaaaaaga taaagataca tggaatagta gtgatgttat
     1201 acgaaatgga aagcactata aaaaaccaca ctatcacgtt atatatattg cacgaaatcc
     1261 tgtaacaata gaaagcgtta ggaacaagat taagcgaaaa ttggggaata gttcagttgc
     1321 tcatgttgag atacttgatt atatcaaagg ttcatatgaa tatttgactc atgaatcaaa
     1381 ggacgctatt gctaagaata aacatatata cgacaaaaaa gatattttga acattaatga
     1441 ttttgatatt gaccgctata taacacttga tgaaagccaa aaaagagaat tgaagaattt
     1501 acttttagat atagtggatg actataattt ggtaaataca aaagatttaa tggcttttat
     1561 tcgccttagg ggagcggagt ttggaatttt aaatacgaat gatgtaaaag atattgtttc
     1621 aacaaactct agcgccttta gattatggtt tgagggcaat tatcagtgtg gatatagagc
     1681 aagttatgca aaggttcttg atgctgaaac gggggaaata aaatgacaaa caaagaaaaa
     1741 gagttatttg ctgaaaatga ggaattaaaa aaagaaatta aggacttaaa agagcgtatt
     1801 gaaagataca gagaaatgga agttgaatta agtacaacaa tagatttatt gagaggaggg
     1861 attattgaat aaataaaagc ccccctgacg aaagtcgaag ggggctttta ttttggtttg
     1921 atgttgcgat taatagcaat acgattgcaa taaacaaaag gatcccctta gaagcaaact
     1981 gctagcgcca cacaaccaca ccacaccaca aataataaaa aagccggatt aataatctgg
     2041 ctttttatat tctctgtcga ccatagaatt atttcctccc gttaaataat agataactat
     2101 taaaaataga caatacttgc tcataagtaa tggtacttaa attgtttact ttggcgtgtt
     2161 tcattgcttg acgaaactga tttttagtaa acagttgacg atattctcga ttgacccatt
     2221 ttgaaacaaa gtacttatat agcttccaat atttatctgg aacatctgtg gtatggcggg
     2281 taagttttat taagacactg tttacttttg gtttaggatg aaagcattcc gctggcagct
     2341 taagcaattg ctgaatcgag acttgagtgt gcaagagcaa ccctagtgtt cggtgaatat
     2401 ccaaggtacg cttgtagaat ccttcttcaa cagtcagata gatgtcagac gcacggcttt
     2461 caaaaaccac ttttttaata atttgtgtgc ttaaattgta aggaatattc ccaacaattt
     2521 tatacctctg tttgttaggg aattgaaact gtagaatatc ttggtgaatt aaagtgacac
     2581 gagtattcag ttttaatttt tctgacgata agttgaatag atgactgtct aattcaatag
     2641 acgttacctg tttacttatt ttagccagtt tcgtcgttaa atgcccttta cctgttccaa
     2701 tttcgtaaac ggtatcggtt tcttttaaat tcaattgttt tattatttgg ttgagtactt
     2761 tttcactcgt taaaaagttt tgagaatatt ttatattttt gttcatgtaa tctctcctga
     2821 agttaaacga tttgtttgta attttagtta tctgtttaaa aagtcataag attagtcact
     2881 ggtaggaatt aatctaacgt atttatttat ctgcgtaatc actgttttta gtctgtttca
     2941 aaacagtaga tgttttatct acattacgca tttggaatac caacatgacg aatccctcct
     3001 tcttaattac aaatttttag catctaattt aacttcaatt cctattttta gcatctaatt
     3061 taacttcaat tcctattata caaaatttta agataatgca ctatcaacac actctgagct
     3121 caaataaagc cctgagttta accgctcggg gctttttgcg tttgccacct gacgtctaag
     3181 aaaaggaata ttcagcaatt tgcccgtgcc gaagaaaggc ccacccgtga aggtgagcca
     3241 gtgagttgat tgctacgtaa ttagttagtt agcccttagt gactcgaatt cgcggccgct
     3301 tctagag
//