LOCUS       BBa_K1036000            2687 bp    DNA     linear       26-Dec-2024
DEFINITION  lux pL controlled luxR with lux pR controlled ndh (LVA-tag) coding 
            for NADH dehydrogenase II
ACCESSION   BBa_K1036000
VERSION     BBa_K1036000.1  
FEATURES             Location/Qualifiers
     misc_feature    1..20
                     /label=LuxR/HSL
     promoter        115..122
                     /label=-10
     promoter        89..94
                     /label=-35
     misc_feature    128..128
                     /label=start
     misc_feature    130..149
                     /label=Putative LuxR/HSL
     misc_feature    1..151
                     /label=BBa_R0063
     promoter        1..151
                     /label=BBa_R0063
     misc_feature    164..167
                     /label=conserved
     RBS             160..171
                     /label=BBa_B0034
     misc_feature    178..179
                     /label=prefix
     misc_feature    351..351
                     /label=T
     misc_feature    669..669
                     /label=A
     CDS             178..927
                     /label=luxR
     misc_feature    178..933
                     /label=BBa_C0062
     misc_feature    934..958
                     /label=Help:Barcodes
     CDS             178..933
                     /label=BBa_C0062
     stem_loop       978..1021
                     /label=stem_loop
     misc_feature    967..1046
                     /label=BBa_B0010
     terminator      967..1046
                     /label=BBa_B0010
     stem_loop       1062..1081
                     /label=T7 TE
     misc_feature    1088..1088
                     /label=stop
     polyA_site      1082..1095
                     /label=polya
     misc_feature    1055..1095
                     /label=BBa_B0012
     terminator      1055..1095
                     /label=BBa_B0012
     misc_feature    1104..1123
                     /label=LuxR/HSL
     promoter        1123..1128
                     /label=-35
     promoter        1145..1150
                     /label=-10
     misc_feature    1156..1156
                     /label=start
     misc_feature    1104..1158
                     /label=BBa_R0062
     promoter        1104..1158
                     /label=BBa_R0062
     misc_feature    1..1158
                     /label=BBa_F2621
     misc_feature    1171..1174
                     /label=conserved
     RBS             1167..1178
                     /label=BBa_B0034
     CDS             1185..2486
                     /label=ndh
     misc_feature    2487..2519
                     /label=LVA
     misc_feature    2520..2525
                     /label=double stop codes
     misc_feature    2526..2550
                     /label=Barcodes
     CDS             1185..2550
                     /label=BBa_K1036001
     stem_loop       2570..2613
                     /label=stem_loop
     misc_feature    2559..2638
                     /label=BBa_B0010
     terminator      2559..2638
                     /label=BBa_B0010
     stem_loop       2654..2673
                     /label=T7 TE
     misc_feature    2680..2680
                     /label=stop
     polyA_site      2674..2687
                     /label=polya
     misc_feature    2647..2687
                     /label=BBa_B0012
     terminator      2647..2687
                     /label=BBa_B0012
ORIGIN
        1 acctgtacga tcctacaggt gcttatgtta agtaattgta ttcccagcga tacaatagtg
       61 tgacaaaaat ccaatttatt agaatcaaat gtcaatccat taccgtttta atgatatata
      121 acacgcaaaa cttgcgacaa acaataggta atactagaga aagaggagaa atactagatg
      181 aaaaacataa atgccgacga cacatacaga ataattaata aaattaaagc ttgtagaagc
      241 aataatgata ttaatcaatg cttatctgat atgactaaaa tggtacattg tgaatattat
      301 ttactcgcga tcatttatcc tcattctatg gttaaatctg atatttcaat cctagataat
      361 taccctaaaa aatggaggca atattatgat gacgctaatt taataaaata tgatcctata
      421 gtagattatt ctaactccaa tcattcacca attaattgga atatatttga aaacaatgct
      481 gtaaataaaa aatctccaaa tgtaattaaa gaagcgaaaa catcaggtct tatcactggg
      541 tttagtttcc ctattcatac ggctaacaat ggcttcggaa tgcttagttt tgcacattca
      601 gaaaaagaca actatataga tagtttattt ttacatgcgt gtatgaacat accattaatt
      661 gttccttctc tagttgataa ttatcgaaaa ataaatatag caaataataa atcaaacaac
      721 gatttaacca aaagagaaaa agaatgttta gcgtgggcat gcgaaggaaa aagctcttgg
      781 gatatttcaa aaatattagg ttgcagtgag cgtactgtca ctttccattt aaccaatgcg
      841 caaatgaaac tcaatacaac aaaccgctgc caaagtattt ctaaagcaat tttaacagga
      901 gcaattgatt gcccatactt taaaaattaa taacactgat agtgctagtg tagatcacta
      961 ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta
     1021 tctgttgttt gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc
     1081 ctttctgcgt ttatatacta gagacctgta ggatcgtaca ggtttacgca agaaaatggt
     1141 ttgttatagt cgaataaata ctagagaaag aggagaaata ctagatgact acgccattga
     1201 aaaaaattgt gattgtcggc ggcggtgctg gtgggctgga aatggcaaca cagctggggc
     1261 ataagctggg acgcaagaaa aaagccaaaa ttacgctggt cgatcgtaac cacagccacc
     1321 tgtggaaacc gctgctgcac gaagtggcga ctggctcgct tgatgaaggc gtcgatgcgt
     1381 tgagctatct ggcccatgcg cgcaatcatg gtttccagtt ccagctgggt tccgtcattg
     1441 atattgatcg tgaagcgaaa acaatcacta ttgcagaact gcgcgacgag aaaggtgaac
     1501 tgctggttcc ggaacgtaaa atcgcctatg acacactggt aatggcgctg ggtagcacct
     1561 ctaacgattt caatacacca ggtgtcaaag agaactgcat tttcctcgat aacccgcacc
     1621 aggcgcgtcg cttccaccag gagatgctga atctgttcct gaaatactcc gccaacctgg
     1681 gcgcgaatgg caaagtgaac attgcgattg tcggcggcgg cgcgacgggt gtagaactct
     1741 ccgctgaatt gcacaacgcg gtcaagcaac tgcacagcta cggttacaaa ggcctgacca
     1801 acgaagccct gaacgtaacg ctggtagaag cgggagaacg tattttgcct gcgttaccgc
     1861 cacgtatctc tgctgcggcc cacaacgagc taacgaaact tggcgttcgc gtgctgacgc
     1921 aaaccatggt caccagtgct gatgaaggcg gcctgcacac taaagatggc gaatatattg
     1981 aggcggatct gatggtatgg gcagccggga tcaaagcgcc agacttcttg aaagatatcg
     2041 gtggtcttga aactaaccgt atcaaccagc tggtggtgga accgacgctg caaaccaccc
     2101 gcgatccaga catttacgct attggcgact gcgcgtcatg cccgcgtccg gaagggggct
     2161 ttgttccgcc gcgtgctcag gctgcacacc agatggcgac ctgcgcaatg aacaatattc
     2221 tggcgcagat aaacggcaag ccgctgaaaa attatcagta taaagatcat ggttcactgg
     2281 tatcgctgtc gaacttctcc accgttggta gcctgatggg taacctgacg cgcggctcaa
     2341 tgatgattga aggacgaatt gcgcgctttg tatatatctc gctataccga atgcatcaga
     2401 ttgcgctgca tggttacttt aaaaccggat taatgatgct ggtggggagt attaaccgcg
     2461 ttatccgtcc gcgtttgaag ttgcatgctg caaacgacga aaactacgct ttagtagctt
     2521 aataactctg atagtgctag tgtagatctc tactagagcc aggcatcaaa taaaacgaaa
     2581 ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcta
     2641 ctagagtcac actggctcac cttcgggtgg gcctttctgc gtttata
//