LOCUS BBa_K1036002 2886 bp DNA linear 22-Dec-2024 DEFINITION gfp (with LVA-tag) under plux R control ACCESSION BBa_K1036002 VERSION BBa_K1036002.1 FEATURES Location/Qualifiers misc_feature 5..8 /label=conserved RBS 1..12 /label=BBa_B0034 misc_feature 19..738 /label=BBa_E0040 misc_feature 733..771 /label=LVA misc_feature 772..777 /label=Double Stop misc_feature 19..21 /label=Start misc_feature 19..777 /label=BBa_J04031 stem_loop 797..840 /label=stem_loop misc_feature 786..865 /label=BBa_B0010 terminator 786..865 /label=BBa_B0010 stem_loop 881..900 /label=T7 TE misc_feature 907..907 /label=stop polyA_site 901..914 /label=polya misc_feature 874..914 /label=BBa_B0012 terminator 874..914 /label=BBa_B0012 misc_feature 1..914 /label=BBa_I763020 misc_feature 923..942 /label=LuxR/HSL promoter 1037..1044 /label=-10 promoter 1011..1016 /label=-35 misc_feature 1050..1050 /label=start misc_feature 1052..1071 /label=Putative LuxR/HSL misc_feature 923..1073 /label=BBa_R0063 promoter 923..1073 /label=BBa_R0063 misc_feature 1086..1089 /label=conserved RBS 1082..1093 /label=BBa_B0034 misc_feature 1100..1101 /label=prefix misc_feature 1273..1273 /label=T misc_feature 1591..1591 /label=A CDS 1100..1849 /label=luxR misc_feature 1100..1855 /label=BBa_C0062 misc_feature 1856..1880 /label=Help:Barcodes CDS 1100..1855 /label=BBa_C0062 stem_loop 1900..1943 /label=stem_loop misc_feature 1889..1968 /label=BBa_B0010 terminator 1889..1968 /label=BBa_B0010 stem_loop 1984..2003 /label=T7 TE misc_feature 2010..2010 /label=stop polyA_site 2004..2017 /label=polya misc_feature 1977..2017 /label=BBa_B0012 terminator 1977..2017 /label=BBa_B0012 misc_feature 2026..2045 /label=LuxR/HSL promoter 2045..2050 /label=-35 promoter 2067..2072 /label=-10 misc_feature 2078..2078 /label=start misc_feature 2026..2080 /label=BBa_R0062 promoter 2026..2080 /label=BBa_R0062 misc_feature 923..2080 /label=BBa_F2621 misc_feature 2093..2096 /label=conserved RBS 2089..2100 /label=BBa_B0034 misc_feature 2686..2717 /label=LVA CDS 2107..2685 /label=luxI misc_feature 2107..2724 /label=BBa_C0061 misc_feature 2725..2749 /label=Help:Barcodes CDS 2107..2724 /label=BBa_C0061 misc_feature 2089..2749 /label=BBa_C0261 stem_loop 2769..2812 /label=stem_loop misc_feature 2758..2837 /label=BBa_B0010 terminator 2758..2837 /label=BBa_B0010 stem_loop 2853..2872 /label=T7 TE misc_feature 2879..2879 /label=stop polyA_site 2873..2886 /label=polya misc_feature 2846..2886 /label=BBa_B0012 terminator 2846..2886 /label=BBa_B0012 terminator 2758..2886 /label=BBa_B0015 misc_feature 2089..2886 /label=BBa_K082014 misc_feature 923..2886 /label=BBa_K546000 ORIGIN 1 aaagaggaga aatactagat gcgtaaagga gaagaacttt tcactggagt tgtcccaatt 61 cttgttgaat tagatggtga tgttaatggg cacaaatttt ctgtcagtgg agagggtgaa 121 ggtgatgcaa catacggaaa acttaccctt aaatttattt gcactactgg aaaactacct 181 gttccatggc caacacttgt cactactttc ggttatggtg ttcaatgctt tgcgagatac 241 ccagatcata tgaaacagca tgactttttc aagagtgcca tgcccgaagg ttatgtacag 301 gaaagaacta tatttttcaa agatgacggg aactacaaga cacgtgctga agtcaagttt 361 gaaggtgata cccttgttaa tagaatcgag ttaaaaggta ttgattttaa agaagatgga 421 aacattcttg gacacaaatt ggaatacaac tataactcac acaatgtata catcatggca 481 gacaaacaaa agaatggaat caaagttaac ttcaaaatta gacacaacat tgaagatgga 541 agcgttcaac tagcagacca ttatcaacaa aatactccaa ttggcgatgg ccctgtcctt 601 ttaccagaca accattacct gtccacacaa tctgcccttt cgaaagatcc caacgaaaag 661 agagaccaca tggtccttct tgagtttgta acagctgctg ggattacaca tggcatggat 721 gaactataca aaaggcctgc tgcaaacgac gaaaactacg ctttagtagc ttaataatac 781 tagagccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat 841 ctgttgtttg tcggtgaacg ctctctacta gagtcacact ggctcacctt cgggtgggcc 901 tttctgcgtt tatatactag agacctgtac gatcctacag gtgcttatgt taagtaattg 961 tattcccagc gatacaatag tgtgacaaaa atccaattta ttagaatcaa atgtcaatcc 1021 attaccgttt taatgatata taacacgcaa aacttgcgac aaacaatagg taatactaga 1081 gaaagaggag aaatactaga tgaaaaacat aaatgccgac gacacataca gaataattaa 1141 taaaattaaa gcttgtagaa gcaataatga tattaatcaa tgcttatctg atatgactaa 1201 aatggtacat tgtgaatatt atttactcgc gatcatttat cctcattcta tggttaaatc 1261 tgatatttca atcctagata attaccctaa aaaatggagg caatattatg atgacgctaa 1321 tttaataaaa tatgatccta tagtagatta ttctaactcc aatcattcac caattaattg 1381 gaatatattt gaaaacaatg ctgtaaataa aaaatctcca aatgtaatta aagaagcgaa 1441 aacatcaggt cttatcactg ggtttagttt ccctattcat acggctaaca atggcttcgg 1501 aatgcttagt tttgcacatt cagaaaaaga caactatata gatagtttat ttttacatgc 1561 gtgtatgaac ataccattaa ttgttccttc tctagttgat aattatcgaa aaataaatat 1621 agcaaataat aaatcaaaca acgatttaac caaaagagaa aaagaatgtt tagcgtgggc 1681 atgcgaagga aaaagctctt gggatatttc aaaaatatta ggttgcagtg agcgtactgt 1741 cactttccat ttaaccaatg cgcaaatgaa actcaataca acaaaccgct gccaaagtat 1801 ttctaaagca attttaacag gagcaattga ttgcccatac tttaaaaatt aataacactg 1861 atagtgctag tgtagatcac tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg 1921 aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcta ctagagtcac 1981 actggctcac cttcgggtgg gcctttctgc gtttatatac tagagacctg taggatcgta 2041 caggtttacg caagaaaatg gtttgttata gtcgaataaa tactagagaa agaggagaaa 2101 tactagatga ctataatgat aaaaaaatcg gattttttgg caattccatc ggaggagtat 2161 aaaggtattc taagtcttcg ttatcaagtg tttaagcaaa gacttgagtg ggacttagtt 2221 gtagaaaata accttgaatc agatgagtat gataactcaa atgcagaata tatttatgct 2281 tgtgatgata ctgaaaatgt aagtggatgc tggcgtttat tacctacaac aggtgattat 2341 atgctgaaaa gtgtttttcc tgaattgctt ggtcaacaga gtgctcccaa agatcctaat 2401 atagtcgaat taagtcgttt tgctgtaggt aaaaatagct caaagataaa taactctgct 2461 agtgaaatta caatgaaact atttgaagct atatataaac acgctgttag tcaaggtatt 2521 acagaatatg taacagtaac atcaacagca atagagcgat ttttaaagcg tattaaagtt 2581 ccttgtcatc gtattggaga caaagaaatt catgtattag gtgatactaa atcggttgta 2641 ttgtctatgc ctattaatga acagtttaaa aaagcagtct taaatgctgc aaacgacgaa 2701 aactacgctt tagtagctta ataactctga tagtgctagt gtagatctct actagagcca 2761 ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt 2821 tgtcggtgaa cgctctctac tagagtcaca ctggctcacc ttcgggtggg cctttctgcg 2881 tttata //