LOCUS BBa_K1036003 4052 bp DNA linear 26-Dec-2024 DEFINITION lux pL controlled luxR with lux pR controlled gfp (LVA-tag) ACCESSION BBa_K1036003 VERSION BBa_K1036003.1 FEATURES Location/Qualifiers misc_feature 1..20 /label=LuxR/HSL promoter 115..122 /label=-10 promoter 89..94 /label=-35 misc_feature 128..128 /label=start misc_feature 130..149 /label=Putative LuxR/HSL misc_feature 1..151 /label=BBa_R0063 promoter 1..151 /label=BBa_R0063 misc_feature 164..167 /label=conserved RBS 160..171 /label=BBa_B0034 misc_feature 178..179 /label=prefix misc_feature 351..351 /label=T misc_feature 669..669 /label=A CDS 178..927 /label=luxR misc_feature 178..933 /label=BBa_C0062 misc_feature 934..958 /label=Help:Barcodes CDS 178..933 /label=BBa_C0062 stem_loop 978..1021 /label=stem_loop misc_feature 967..1046 /label=BBa_B0010 terminator 967..1046 /label=BBa_B0010 stem_loop 1062..1081 /label=T7 TE misc_feature 1088..1088 /label=stop polyA_site 1082..1095 /label=polya misc_feature 1055..1095 /label=BBa_B0012 terminator 1055..1095 /label=BBa_B0012 misc_feature 1104..1123 /label=LuxR/HSL promoter 1123..1128 /label=-35 promoter 1145..1150 /label=-10 misc_feature 1156..1156 /label=start misc_feature 1104..1158 /label=BBa_R0062 promoter 1104..1158 /label=BBa_R0062 misc_feature 1..1158 /label=BBa_F2621 misc_feature 1171..1174 /label=conserved RBS 1167..1178 /label=BBa_B0034 misc_feature 1185..1904 /label=BBa_E0040 misc_feature 1899..1937 /label=LVA misc_feature 1938..1943 /label=Double Stop misc_feature 1185..1187 /label=Start misc_feature 1185..1943 /label=BBa_J04031 stem_loop 1963..2006 /label=stem_loop misc_feature 1952..2031 /label=BBa_B0010 terminator 1952..2031 /label=BBa_B0010 stem_loop 2047..2066 /label=T7 TE misc_feature 2073..2073 /label=stop polyA_site 2067..2080 /label=polya misc_feature 2040..2080 /label=BBa_B0012 terminator 2040..2080 /label=BBa_B0012 misc_feature 1167..2080 /label=BBa_I763020 misc_feature 2089..2108 /label=LuxR/HSL promoter 2203..2210 /label=-10 promoter 2177..2182 /label=-35 misc_feature 2216..2216 /label=start misc_feature 2218..2237 /label=Putative LuxR/HSL misc_feature 2089..2239 /label=BBa_R0063 promoter 2089..2239 /label=BBa_R0063 misc_feature 2252..2255 /label=conserved RBS 2248..2259 /label=BBa_B0034 misc_feature 2266..2267 /label=prefix misc_feature 2439..2439 /label=T misc_feature 2757..2757 /label=A CDS 2266..3015 /label=luxR misc_feature 2266..3021 /label=BBa_C0062 misc_feature 3022..3046 /label=Help:Barcodes CDS 2266..3021 /label=BBa_C0062 stem_loop 3066..3109 /label=stem_loop misc_feature 3055..3134 /label=BBa_B0010 terminator 3055..3134 /label=BBa_B0010 stem_loop 3150..3169 /label=T7 TE misc_feature 3176..3176 /label=stop polyA_site 3170..3183 /label=polya misc_feature 3143..3183 /label=BBa_B0012 terminator 3143..3183 /label=BBa_B0012 misc_feature 3192..3211 /label=LuxR/HSL promoter 3211..3216 /label=-35 promoter 3233..3238 /label=-10 misc_feature 3244..3244 /label=start misc_feature 3192..3246 /label=BBa_R0062 promoter 3192..3246 /label=BBa_R0062 misc_feature 2089..3246 /label=BBa_F2621 misc_feature 3259..3262 /label=conserved RBS 3255..3266 /label=BBa_B0034 misc_feature 3852..3883 /label=LVA CDS 3273..3851 /label=luxI misc_feature 3273..3890 /label=BBa_C0061 misc_feature 3891..3915 /label=Help:Barcodes CDS 3273..3890 /label=BBa_C0061 misc_feature 3255..3915 /label=BBa_C0261 stem_loop 3935..3978 /label=stem_loop misc_feature 3924..4003 /label=BBa_B0010 terminator 3924..4003 /label=BBa_B0010 stem_loop 4019..4038 /label=T7 TE misc_feature 4045..4045 /label=stop polyA_site 4039..4052 /label=polya misc_feature 4012..4052 /label=BBa_B0012 terminator 4012..4052 /label=BBa_B0012 terminator 3924..4052 /label=BBa_B0015 misc_feature 3255..4052 /label=BBa_K082014 misc_feature 2089..4052 /label=BBa_K546000 ORIGIN 1 acctgtacga tcctacaggt gcttatgtta agtaattgta ttcccagcga tacaatagtg 61 tgacaaaaat ccaatttatt agaatcaaat gtcaatccat taccgtttta atgatatata 121 acacgcaaaa cttgcgacaa acaataggta atactagaga aagaggagaa atactagatg 181 aaaaacataa atgccgacga cacatacaga ataattaata aaattaaagc ttgtagaagc 241 aataatgata ttaatcaatg cttatctgat atgactaaaa tggtacattg tgaatattat 301 ttactcgcga tcatttatcc tcattctatg gttaaatctg atatttcaat cctagataat 361 taccctaaaa aatggaggca atattatgat gacgctaatt taataaaata tgatcctata 421 gtagattatt ctaactccaa tcattcacca attaattgga atatatttga aaacaatgct 481 gtaaataaaa aatctccaaa tgtaattaaa gaagcgaaaa catcaggtct tatcactggg 541 tttagtttcc ctattcatac ggctaacaat ggcttcggaa tgcttagttt tgcacattca 601 gaaaaagaca actatataga tagtttattt ttacatgcgt gtatgaacat accattaatt 661 gttccttctc tagttgataa ttatcgaaaa ataaatatag caaataataa atcaaacaac 721 gatttaacca aaagagaaaa agaatgttta gcgtgggcat gcgaaggaaa aagctcttgg 781 gatatttcaa aaatattagg ttgcagtgag cgtactgtca ctttccattt aaccaatgcg 841 caaatgaaac tcaatacaac aaaccgctgc caaagtattt ctaaagcaat tttaacagga 901 gcaattgatt gcccatactt taaaaattaa taacactgat agtgctagtg tagatcacta 961 ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta 1021 tctgttgttt gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc 1081 ctttctgcgt ttatatacta gagacctgta ggatcgtaca ggtttacgca agaaaatggt 1141 ttgttatagt cgaataaata ctagagaaag aggagaaata ctagatgcgt aaaggagaag 1201 aacttttcac tggagttgtc ccaattcttg ttgaattaga tggtgatgtt aatgggcaca 1261 aattttctgt cagtggagag ggtgaaggtg atgcaacata cggaaaactt acccttaaat 1321 ttatttgcac tactggaaaa ctacctgttc catggccaac acttgtcact actttcggtt 1381 atggtgttca atgctttgcg agatacccag atcatatgaa acagcatgac tttttcaaga 1441 gtgccatgcc cgaaggttat gtacaggaaa gaactatatt tttcaaagat gacgggaact 1501 acaagacacg tgctgaagtc aagtttgaag gtgataccct tgttaataga atcgagttaa 1561 aaggtattga ttttaaagaa gatggaaaca ttcttggaca caaattggaa tacaactata 1621 actcacacaa tgtatacatc atggcagaca aacaaaagaa tggaatcaaa gttaacttca 1681 aaattagaca caacattgaa gatggaagcg ttcaactagc agaccattat caacaaaata 1741 ctccaattgg cgatggccct gtccttttac cagacaacca ttacctgtcc acacaatctg 1801 ccctttcgaa agatcccaac gaaaagagag accacatggt ccttcttgag tttgtaacag 1861 ctgctgggat tacacatggc atggatgaac tatacaaaag gcctgctgca aacgacgaaa 1921 actacgcttt agtagcttaa taatactaga gccaggcatc aaataaaacg aaaggctcag 1981 tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct ctactagagt 2041 cacactggct caccttcggg tgggcctttc tgcgtttata tactagagac ctgtacgatc 2101 ctacaggtgc ttatgttaag taattgtatt cccagcgata caatagtgtg acaaaaatcc 2161 aatttattag aatcaaatgt caatccatta ccgttttaat gatatataac acgcaaaact 2221 tgcgacaaac aataggtaat actagagaaa gaggagaaat actagatgaa aaacataaat 2281 gccgacgaca catacagaat aattaataaa attaaagctt gtagaagcaa taatgatatt 2341 aatcaatgct tatctgatat gactaaaatg gtacattgtg aatattattt actcgcgatc 2401 atttatcctc attctatggt taaatctgat atttcaatcc tagataatta ccctaaaaaa 2461 tggaggcaat attatgatga cgctaattta ataaaatatg atcctatagt agattattct 2521 aactccaatc attcaccaat taattggaat atatttgaaa acaatgctgt aaataaaaaa 2581 tctccaaatg taattaaaga agcgaaaaca tcaggtctta tcactgggtt tagtttccct 2641 attcatacgg ctaacaatgg cttcggaatg cttagttttg cacattcaga aaaagacaac 2701 tatatagata gtttattttt acatgcgtgt atgaacatac cattaattgt tccttctcta 2761 gttgataatt atcgaaaaat aaatatagca aataataaat caaacaacga tttaaccaaa 2821 agagaaaaag aatgtttagc gtgggcatgc gaaggaaaaa gctcttggga tatttcaaaa 2881 atattaggtt gcagtgagcg tactgtcact ttccatttaa ccaatgcgca aatgaaactc 2941 aatacaacaa accgctgcca aagtatttct aaagcaattt taacaggagc aattgattgc 3001 ccatacttta aaaattaata acactgatag tgctagtgta gatcactact agagccaggc 3061 atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt 3121 cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct ttctgcgttt 3181 atatactaga gacctgtagg atcgtacagg tttacgcaag aaaatggttt gttatagtcg 3241 aataaatact agagaaagag gagaaatact agatgactat aatgataaaa aaatcggatt 3301 ttttggcaat tccatcggag gagtataaag gtattctaag tcttcgttat caagtgttta 3361 agcaaagact tgagtgggac ttagttgtag aaaataacct tgaatcagat gagtatgata 3421 actcaaatgc agaatatatt tatgcttgtg atgatactga aaatgtaagt ggatgctggc 3481 gtttattacc tacaacaggt gattatatgc tgaaaagtgt ttttcctgaa ttgcttggtc 3541 aacagagtgc tcccaaagat cctaatatag tcgaattaag tcgttttgct gtaggtaaaa 3601 atagctcaaa gataaataac tctgctagtg aaattacaat gaaactattt gaagctatat 3661 ataaacacgc tgttagtcaa ggtattacag aatatgtaac agtaacatca acagcaatag 3721 agcgattttt aaagcgtatt aaagttcctt gtcatcgtat tggagacaaa gaaattcatg 3781 tattaggtga tactaaatcg gttgtattgt ctatgcctat taatgaacag tttaaaaaag 3841 cagtcttaaa tgctgcaaac gacgaaaact acgctttagt agcttaataa ctctgatagt 3901 gctagtgtag atctctacta gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga 3961 ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctctactaga gtcacactgg 4021 ctcaccttcg ggtgggcctt tctgcgttta ta //