##gff-version 3
##sequence-region BBa_K1036003 1 4052
BBa_K1036003	.	engineered_region	1	1158	.	+	0	ID=BBa_F2621;Name=BBa_F2621
BBa_K1036003	.	promoter	1104	1158	.	+	0	ID=BBa_R0062;Name=BBa_R0062;Parent=BBa_F2621
BBa_K1036003	.	non_covalent_binding_site	1104	1123	.	+	0	ID=annotation2045;Name=LuxR/HSL;Parent=BBa_R0062
BBa_K1036003	.	promoter	1145	1150	.	+	0	ID=annotation2047;Name=-10;Parent=BBa_R0062
BBa_K1036003	.	engineered_region	1104	1158	.	+	0	ID=annotation7070;Name=BBa_R0062;Parent=BBa_R0062
BBa_K1036003	.	start_codon	1156	1156	.	+	0	ID=annotation2048;Name=start;Parent=BBa_R0062
BBa_K1036003	.	promoter	1123	1128	.	+	0	ID=annotation2046;Name=-35;Parent=BBa_R0062
BBa_K1036003	.	CDS	178	933	.	+	0	ID=BBa_C0062;Name=BBa_C0062;Parent=BBa_F2621
BBa_K1036003	.	CDS	178	927	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_K1036003	.	engineered_region	178	933	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_K1036003	.	engineered_tag	934	958	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_K1036003	.	point_mutation	351	351	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_K1036003	.	engineered_region	178	179	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_K1036003	.	point_mutation	669	669	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_K1036003	.	terminator	1055	1095	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_F2621
BBa_K1036003	.	engineered_region	1055	1095	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1036003	.	stop_codon	1088	1088	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1036003	.	stem_loop	1062	1081	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1036003	.	polyA_site	1082	1095	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1036003	.	promoter	1	151	.	+	0	ID=BBa_R0063;Name=BBa_R0063;Parent=BBa_F2621
BBa_K1036003	.	engineered_region	1	151	.	+	0	ID=annotation7071;Name=BBa_R0063;Parent=BBa_R0063
BBa_K1036003	.	start_codon	128	128	.	+	0	ID=annotation2054;Name=start;Parent=BBa_R0063
BBa_K1036003	.	non_covalent_binding_site	1	20	.	+	0	ID=annotation2051;Name=LuxR/HSL;Parent=BBa_R0063
BBa_K1036003	.	non_covalent_binding_site	130	149	.	+	0	ID=annotation2055;Name=Putative LuxR/HSL;Parent=BBa_R0063
BBa_K1036003	.	promoter	89	94	.	+	0	ID=annotation2053;Name=-35;Parent=BBa_R0063
BBa_K1036003	.	promoter	115	122	.	+	0	ID=annotation2052;Name=-10;Parent=BBa_R0063
BBa_K1036003	.	terminator	967	1046	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_F2621
BBa_K1036003	.	stem_loop	978	1021	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1036003	.	engineered_region	967	1046	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1036003	.	ribosome_entry_site	160	171	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_F2621
BBa_K1036003	.	conserved_region	164	167	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1036003	.	engineered_region	2089	4052	.	+	0	ID=BBa_K546000;Name=BBa_K546000
BBa_K1036003	.	engineered_region	3255	4052	.	+	0	ID=BBa_K082014;Name=BBa_K082014;Parent=BBa_K546000
BBa_K1036003	.	terminator	3924	4052	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K082014
BBa_K1036003	.	terminator	3924	4003	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K1036003	.	stem_loop	3935	3978	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1036003	.	engineered_region	3924	4003	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1036003	.	terminator	4012	4052	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K1036003	.	engineered_region	4012	4052	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1036003	.	stop_codon	4045	4045	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1036003	.	stem_loop	4019	4038	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1036003	.	polyA_site	4039	4052	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1036003	.	engineered_region	3255	3915	.	+	0	ID=BBa_C0261;Name=BBa_C0261;Parent=BBa_K082014
BBa_K1036003	.	CDS	3273	3890	.	+	0	ID=BBa_C0061;Name=BBa_C0061;Parent=BBa_C0261
BBa_K1036003	.	engineered_region	3273	3890	.	+	0	ID=annotation7038;Name=BBa_C0061;Parent=BBa_C0061
BBa_K1036003	.	tag	3852	3883	.	+	0	ID=annotation1760;Name=LVA;Parent=BBa_C0061
BBa_K1036003	.	engineered_tag	3891	3915	.	+	0	ID=annotation2213985;Name=Help:Barcodes;Parent=BBa_C0061
BBa_K1036003	.	CDS	3273	3851	.	+	0	ID=annotation1761;Name=luxI;Parent=BBa_C0061
BBa_K1036003	.	ribosome_entry_site	3255	3266	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_C0261
BBa_K1036003	.	conserved_region	3259	3262	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1036003	.	engineered_region	2089	3246	.	+	0	ID=BBa_F2621;Name=BBa_F2621;Parent=BBa_K546000
BBa_K1036003	.	promoter	3192	3246	.	+	0	ID=BBa_R0062;Name=BBa_R0062;Parent=BBa_F2621
BBa_K1036003	.	non_covalent_binding_site	3192	3211	.	+	0	ID=annotation2045;Name=LuxR/HSL;Parent=BBa_R0062
BBa_K1036003	.	promoter	3233	3238	.	+	0	ID=annotation2047;Name=-10;Parent=BBa_R0062
BBa_K1036003	.	engineered_region	3192	3246	.	+	0	ID=annotation7070;Name=BBa_R0062;Parent=BBa_R0062
BBa_K1036003	.	start_codon	3244	3244	.	+	0	ID=annotation2048;Name=start;Parent=BBa_R0062
BBa_K1036003	.	promoter	3211	3216	.	+	0	ID=annotation2046;Name=-35;Parent=BBa_R0062
BBa_K1036003	.	CDS	2266	3021	.	+	0	ID=BBa_C0062;Name=BBa_C0062;Parent=BBa_F2621
BBa_K1036003	.	CDS	2266	3015	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_K1036003	.	engineered_region	2266	3021	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_K1036003	.	engineered_tag	3022	3046	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_K1036003	.	point_mutation	2439	2439	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_K1036003	.	engineered_region	2266	2267	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_K1036003	.	point_mutation	2757	2757	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_K1036003	.	terminator	3143	3183	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_F2621
BBa_K1036003	.	engineered_region	3143	3183	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1036003	.	stop_codon	3176	3176	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1036003	.	stem_loop	3150	3169	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1036003	.	polyA_site	3170	3183	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1036003	.	promoter	2089	2239	.	+	0	ID=BBa_R0063;Name=BBa_R0063;Parent=BBa_F2621
BBa_K1036003	.	engineered_region	2089	2239	.	+	0	ID=annotation7071;Name=BBa_R0063;Parent=BBa_R0063
BBa_K1036003	.	start_codon	2216	2216	.	+	0	ID=annotation2054;Name=start;Parent=BBa_R0063
BBa_K1036003	.	non_covalent_binding_site	2089	2108	.	+	0	ID=annotation2051;Name=LuxR/HSL;Parent=BBa_R0063
BBa_K1036003	.	non_covalent_binding_site	2218	2237	.	+	0	ID=annotation2055;Name=Putative LuxR/HSL;Parent=BBa_R0063
BBa_K1036003	.	promoter	2177	2182	.	+	0	ID=annotation2053;Name=-35;Parent=BBa_R0063
BBa_K1036003	.	promoter	2203	2210	.	+	0	ID=annotation2052;Name=-10;Parent=BBa_R0063
BBa_K1036003	.	terminator	3055	3134	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_F2621
BBa_K1036003	.	stem_loop	3066	3109	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1036003	.	engineered_region	3055	3134	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1036003	.	ribosome_entry_site	2248	2259	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_F2621
BBa_K1036003	.	conserved_region	2252	2255	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K1036003	.	engineered_region	1167	2080	.	+	0	ID=BBa_I763020;Name=BBa_I763020
BBa_K1036003	.	terminator	1952	2031	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_I763020
BBa_K1036003	.	stem_loop	1963	2006	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K1036003	.	engineered_region	1952	2031	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K1036003	.	terminator	2040	2080	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_I763020
BBa_K1036003	.	engineered_region	2040	2080	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K1036003	.	stop_codon	2073	2073	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K1036003	.	stem_loop	2047	2066	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K1036003	.	polyA_site	2067	2080	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K1036003	.	engineered_region	1185	1943	.	+	0	ID=BBa_J04031;Name=BBa_J04031;Parent=BBa_I763020
BBa_K1036003	.	engineered_region	1185	1904	.	+	0	ID=annotation1507202;Name=BBa_E0040;Parent=BBa_J04031
BBa_K1036003	.	tag	1899	1937	.	+	0	ID=annotation1507235;Name=LVA;Parent=BBa_J04031
BBa_K1036003	.	stop_codon	1938	1943	.	+	0	ID=annotation1507242;Name=Double Stop;Parent=BBa_J04031
BBa_K1036003	.	start_codon	1185	1187	.	+	0	ID=annotation1507246;Name=Start;Parent=BBa_J04031
BBa_K1036003	.	ribosome_entry_site	1167	1178	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I763020
BBa_K1036003	.	conserved_region	1171	1174	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
>BBa_K1036003
acctgtacgatcctacaggtgcttatgttaagtaattgtattcccagcgatacaatagtgtgacaaaaatccaatttat
tagaatcaaatgtcaatccattaccgttttaatgatatataacacgcaaaacttgcgacaaacaataggtaatactaga
gaaagaggagaaatactagatgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtaga
agcaataatgatattaatcaatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcattt
atcctcattctatggttaaatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgc
taatttaataaaatatgatcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaac
aatgctgtaaataaaaaatctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttcccta
ttcatacggctaacaatggcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttattttt
acatgcgtgtatgaacataccattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaa
tcaaacaacgatttaaccaaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaa
aaatattaggttgcagtgagcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctg
ccaaagtatttctaaagcaattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctag
tgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttg
tttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagacc
tgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaataaatactagagaaagaggagaaatactaga
tgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaatt
ttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaacta
cctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaac
agcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaacta
caagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaa
gatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaaga
atggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaa
tactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatccc
aacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactataca
aaaggcctgctgcaaacgacgaaaactacgctttagtagcttaataatactagagccaggcatcaaataaaacgaaagg
ctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcacc
ttcgggtgggcctttctgcgtttatatactagagacctgtacgatcctacaggtgcttatgttaagtaattgtattccc
agcgatacaatagtgtgacaaaaatccaatttattagaatcaaatgtcaatccattaccgttttaatgatatataacac
gcaaaacttgcgacaaacaataggtaatactagagaaagaggagaaatactagatgaaaaacataaatgccgacgacac
atacagaataattaataaaattaaagcttgtagaagcaataatgatattaatcaatgcttatctgatatgactaaaatg
gtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaaatctgatatttcaatcctagataatt
accctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcctatagtagattattctaactccaa
tcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatctccaaatgtaattaaagaagcgaaa
acatcaggtcttatcactgggtttagtttccctattcatacggctaacaatggcttcggaatgcttagttttgcacatt
cagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacataccattaattgttccttctctagttga
taattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaatgtttagcgtgg
gcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgtactgtcactttccatttaacca
atgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaattttaacaggagcaattgattgccc
atactttaaaaattaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctc
agtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttc
gggtgggcctttctgcgtttatatactagagacctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtc
gaataaatactagagaaagaggagaaatactagatgactataatgataaaaaaatcggattttttggcaattccatcgg
aggagtataaaggtattctaagtcttcgttatcaagtgtttaagcaaagacttgagtgggacttagttgtagaaaataa
ccttgaatcagatgagtatgataactcaaatgcagaatatatttatgcttgtgatgatactgaaaatgtaagtggatgc
tggcgtttattacctacaacaggtgattatatgctgaaaagtgtttttcctgaattgcttggtcaacagagtgctccca
aagatcctaatatagtcgaattaagtcgttttgctgtaggtaaaaatagctcaaagataaataactctgctagtgaaat
tacaatgaaactatttgaagctatatataaacacgctgttagtcaaggtattacagaatatgtaacagtaacatcaaca
gcaatagagcgatttttaaagcgtattaaagttccttgtcatcgtattggagacaaagaaattcatgtattaggtgata
ctaaatcggttgtattgtctatgcctattaatgaacagtttaaaaaagcagtcttaaatgctgcaaacgacgaaaacta
cgctttagtagcttaataactctgatagtgctagtgtagatctctactagagccaggcatcaaataaaacgaaaggctc
agtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttc
gggtgggcctttctgcgtttata